- Volume 51(6); December 2013
-
Reviews
- MINIREVIEW] Overview: Replication of Porcine Reproductive and Respiratory Syndrome Virus
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Sang-Im Yun , Young-Min Lee
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J. Microbiol. 2013;51(6):711-723. Published online December 19, 2013
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DOI: https://doi.org/10.1007/s12275-013-3431-z
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337
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Abstract
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Porcine reproductive and respiratory syndrome virus (PRRSV),
an arterivirus that causes significant losses in the pig industry,
is one of the most important animal pathogens of global
significance. Since the discovery of the virus, significant progress
has been made in understanding its epidemiology and
transmission, but no adequate control measures are yet available
to eliminate infection with this pathogen. The genome
replication of PRRSV is required to reproduce, within a few
hours of infection, the millions of progeny virions that establish,
disseminate, and maintain infection. Replication of the
viral RNA genome is a multistep process involving a replication
complex that is formed not only from components
of viral and cellular origin but also from the viral genomic
RNA template; this replication complex is embedded within
particular virus-induced membrane vesicles. PRRSV RNA
replication is directed by at least 14 replicase proteins that
have both common enzymatic activities, including viral RNA
polymerase, and also unusual and poorly understood RNAprocessing
functions. In this review, we summarize our
current understanding of PRRSV replication, which is important
for developing a successful strategy for the prevention
and control of this pathogen.
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Citations
Citations to this article as recorded by

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- MINIREVIEW] Shiga Toxins Expressed by Human Pathogenic Bacteria Induce Immune Responses in Host Cells
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Moo-Seung Lee , Myung Hee Kim , Vernon L. Tesh
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J. Microbiol. 2013;51(6):724-730. Published online December 19, 2013
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DOI: https://doi.org/10.1007/s12275-013-3429-6
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341
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Abstract
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Shiga toxins are a family of genetically and structurally related
toxins that are the primary virulence factors produced
by the bacterial pathogens Shigella dysenteriae serotype 1
and certain Escherichia coli strains. The toxins are multifunctional
proteins inducing protein biosynthesis inhibition,
ribotoxic and ER stress responses, apoptosis, autophagy, and
inflammatory cytokine and chemokine production. The regulated
induction of inflammatory responses is key to minimizing
damage upon injury or pathogen-mediated infections,
requiring the concerted activation of multiple signaling pathways
to control cytokine/chemokine expression. Activation
of host cell signaling cascades is essential for Shiga toxinmediated
proinflammatory responses and the contribution
of the toxins to virulence. Many studies have been reported
defining the inflammatory response to Shiga toxins in vivo
and in vitro, including production and secretion of tumor
necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), macrophage
inflammatory protein-1α/β (MIP-1α/β), macrophage
chemoattractant monocyte chemoattractant protein
1 (MCP-1), interleukin 8 (IL-8), interleukin 6 (IL-6), and
Groβ. These cytokines and chemokines may contribute to
damage in the colon and development of life threatening
conditions such as acute renal failure (hemolytic uremic
syndrome) and neurological abnormalities. In this review,
we summarize recent findings in Shiga toxin-mediated inflammatory
responses by different types of cells in vitro and
in animal models. Signaling pathways involved in the inflammatory
responses are briefly reviewed.
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Citations
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Research Support, Non-U.S. Gov'ts
- Niabella terrae sp. nov. Isolated from Greenhouse Soil
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Jae-Hyung Ahn , Eun-Hye Jo , Byung-Yong Kim , Jaekyeong Song , Soon-Wo Kwon , Hang-Yeon Weon
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J. Microbiol. 2013;51(6):731-735. Published online December 19, 2013
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DOI: https://doi.org/10.1007/s12275-013-3507-9
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352
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6
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Abstract
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An orange-colored bacterial strain, ICM 1-15T, was isolated
from greenhouse soil. The 16S rRNA gene sequence of this
strain showed the highest sequence similarity with Niabella
ginsengisoli GR10-1T (95.2%) and Niabella yanshanensis
CCBAU 05354T (95.0%) among the type strains. The strain
ICM 1-15T was a strictly aerobic, Gram-negative, non-sporeforming,
non-motile, flexirubin pigment-producing, short
rod-shaped bacterium. The strain grew at 15–35°C (optimum,
25°C), at a pH of 5.0–8.5 (optimum, pH 6.5), and in
the presence of 0–3% NaCl (optimum, 1%). The DNA G+C
content of strain ICM 1-15T was 43.6 mol%. It contained
MK-7 as the major isoprenoid quinone and iso-C15:0 (38.9%),
iso-C15:1 G (20.3%), and iso-C17:0 3-OH (12.9%) as the major
fatty acids. On the basis of evidence from our polyphasic
taxonomic study, we concluded that strain ICM 1-15T should
be classified within a novel species of the genus Niabella,
for which the name Niabella terrae sp. nov. is proposed.
The type strain is ICM 1-15T (=KACC 17443T =JCM 19502T).
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Citations
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Niabella defluvii sp. nov., isolated from influent water of a wastewater treatment plant
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- Aestuariibaculum scopimerae sp. nov., Isolated from the Globular Ghost Crab, Scopimera globosa
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Jae-Bong Lee , Byung-Chun Kim , Hyangmi Kim , Kyung Sook Bae , Jae-Hyeong Yang , Young-Yull Chun , Seong-Joon Park , Doo-Sang Park
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J. Microbiol. 2013;51(6):736-740. Published online December 19, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3499-5
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352
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0
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4
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Abstract
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A Gram-staining-negative, non-motile, catalase- and oxidasepositive
bacterium, designated strain I-15T, was isolated
from a crab of the Yellow Sea, Korea. On the basis of a 16S
rRNA gene sequence analysis, strain I-15T was shown to
belong to Bacteroidetes, related to the genus Aestuariibaculum.
Sequence similarity between strain I-15T and the only type
strain of the genus Aestuariibaculum, Aestuariibaculum suncheonense
SC17T, was 96.7%. Strain I-15T grew at 0.5–6.0%
(w/v) NaCl, at 10–42°C and at pH 4.5–8.0. It could hydrolyze
starch and Tweens 80. Menaquinone-6 was the only respiratory
quinone, and summed features 3 (C16:1 ω7c/C16:1 ω6c)
(16.4%), iso-C15:0 (15.6%), and iso-C15:1 G (12.6%) were the
major cellular fatty acids. The major polar lipids were phosphatidylethanolamine,
two unidentified aminolipids and two
unidentified lipids. The DNA G+C content was 39.0 mol%.
Polyphasic data allowed genotypic and phenotypic distinction
of strain I-15T from the only validly published Aestuariibaculum
species. Therefore, the organism is considered a
novel species of the genus Aestuariibaculum, for which the
name Aestuariibaculum scopimerae sp. nov. is proposed.
The type strain is I-15T (=KCTC 32459T =JCM 19486T).
-
Citations
Citations to this article as recorded by

- Aestuariibaculum lutulentum sp. nov., a marine bacterium isolated from coastal sediment in Beihai
Jia-Wei Gao, Dong-Yan He, Wen-Wu Zhang, Yu-Ruo Wang, Yue Su, Jun-Jie Ying, Zhi-Cheng Wu, Wu Qu, Lin Xu, Cong Sun
Archives of Microbiology.2023;[Epub] CrossRef - Complete genome sequence of carotenoid-producing Aestuariibaculum lutulentum L182T isolated from the tidal sediment
Wen-Jia Liu, Jia-Wei Gao, Yu Zhang, Cong Sun, Lin Xu
Marine Genomics.2023; 72: 101074. CrossRef - Aestuariibaculum sediminum sp. nov., a marine bacterium isolated from a tidal flat in Zhoushan
Zhi-Cheng Wu, Xin-Yin Zhang, Cong Sun, Lin Xu, Ge-Yi Fu, Xue-Wei Xu
Archives of Microbiology.2021; 203(6): 2953. CrossRef - List of new names and new combinations that have appeared in effective publications outside of the IJSEM and are submitted for valid publication
Aharon Oren, George M. Garrity
International Journal of Systematic and Evolutionary Microbiology
.2019;[Epub] CrossRef
- Sunxiuqinia dokdonensis sp. nov., Isolated from Deep Sub-Seafloor Sediment
-
Dong-Ho Chang , Jae-Bong Lee , Geun-Hye Lee , Moon-Soo Rhee , Haewon Lee , Kyung Sook Bae , Doo-Sang Park , Byoung-Chan Kim
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J. Microbiol. 2013;51(6):741-746. Published online December 19, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3492-z
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456
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0
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10
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Abstract
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A novel facultatively anaerobic strain DH1T was isolated
from deep sub-seafloor sediment at a depth of 900 m below
the seafloor off Seo-do (the west part of Dokdo Island) in
the East Sea of the Republic of Korea. The new strain was
characterized using polyphasic approaches. The isolate was
Gram-stain-negative, motile by gliding, non-spore-forming
rods, oxidase-negative, and catalase-positive; and formed
colonies of orange-red color. The NaCl range for growth
was 0.5–7.0% (w/v) and no growth was observed in the absence
of NaCl. The isolate grew optimally at 30°C, with 2%
(w/v) NaCl and at pH 7. The cell-wall hydrolysates contained
ribose as a major sugar. The DNA G+C content was 40.8
mol%. The closest related strains are Sunxiuqinia faeciviva
JAM-BA0302T and Sunxiuqinia elliptica DQHS-4T (97.9 and
96.3% sequence similarity, respectively). The level of DNADNA
relatedness between strain DH1T and S. faeciviva JAMBA0302T
was around 41% (but only 6% between DH1T and
S. elliptica DQHS-4T). The major cellular fatty acids of the
isolate were contained iso-C15:0 (25.9%), anteiso-C15:0 (16.7%),
and summed feature 9 (comprising C16:0 3-OH and/or unknown
fatty acid of dimethylacetal ECL 17.157; 13.2%).
The predominant menaquinone was MK-7. On the basis of
polyphasic evidence from this study, the isolate was considered
to represent a novel species of the genus Sunxiuqinia,
for which the name Sunxiuqinia dokdonensis sp. nov. is
proposed; the type strain is DH1T (=KCTC 32503T =CGMCC
1.12676T =JCM 19380T).
-
Citations
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Gaoshiqia hydrogeniformans sp. nov., a novel hydrogen-producing bacterium isolated from a deep diatomaceous shale formation
Akio Ueno, Kiyoshi Sato, Shuji Tamamura, Takuma Murakami, Hidenori Inomata, Satoshi Tamazawa, Yuki Amano, Kazuya Miyakawa, Takeshi Naganuma, Toshifumi Igarashi
International Journal of Systematic and Evolutionary Microbiology
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Nicole Adam, Yuchen Han, Katja Laufer-Meiser, Rebecca Bährle, Ulrich Schwarz-Schampera, Axel Schippers, Mirjam Perner
Frontiers in Microbiology.2021;[Epub] CrossRef -
Sunxiuqinia indica sp. nov., isolated from deep sea
Jianyang Li, Mingming Qi, Qiliang Lai, Guangyi Wang, Zongze Shao
International Journal of Systematic and Evolutionary Microbiology
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Zhaobin Huang, Yuzhong Hu, Qiliang Lai, Yu Guo
Systematic and Applied Microbiology.2020; 43(4): 126099. CrossRef - Simulated reactive zone with emulsified vegetable oil for the long-term remediation of Cr(VI)-contaminated aquifer: dynamic evolution of geological parameters and groundwater microbial community
Jun Dong, Jinqiu Yu, Qiburi Bao
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Sooyeon Lim, Dong-Ho Chang, Byoung-Chan Kim
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Y. Dai, Z. Yan, L. Jia, S. Zhang, L. Gao, X. Wei, Z. Mei, X. Liu
Journal of Applied Microbiology.2016; 121(1): 163. CrossRef -
Roseimarinus sediminis gen. nov., sp. nov., a facultatively anaerobic bacterium isolated from coastal sediment
Wen-Jie Wu, Qian-Qian Liu, Guan-Jun Chen, Zong-Jun Du
International Journal of Systematic and Evolutionary Microbiology
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Byung-Ho Bang, Moon-Soo Rhee, Dong-Ho Chang, Doo-Sang Park, Byoung-Chan Kim
Antonie van Leeuwenhoek.2015; 107(2): 443. CrossRef - Bacterial colonization of a fumigated alkaline saline soil
Juan M. Bello-López, Cristina A. Domínguez-Mendoza, Arit S. de León-Lorenzana, Laura Delgado-Balbuena, Yendi E. Navarro-Noya, Selene Gómez-Acata, Analine Rodríguez-Valentín, Victor M. Ruíz-Valdiviezo, Marco Luna-Guido, Nele Verhulst, Bram Govaerts, Luc De
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- Diversity of the Bacterial Community in the Rice Rhizosphere Managed Under Conventional and No-tillage Practices
-
Zubair Aslam , Muhammad Yasir , Hwan Sik Yoon , Che Ok Jeon , Young Ryun Chung
-
J. Microbiol. 2013;51(6):747-756. Published online December 19, 2013
-
DOI: https://doi.org/10.1007/s12275-013-2528-8
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368
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0
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27
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Abstract
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Bacterial diversity in the rice rhizosphere at different rice
growth stages, managed under conventional and no-tillage
practices, was explored using a culture-based approach.
Actinobacteria are among the bacterial phyla abundant in
the rice rhizosphere. Their diversity was further examined by
constructing metagenomic libraries based on the 16S rRNA
gene, using actinobacterial- and streptomycete-specific polymerase
chain reaction (PCR) primers. The study included
132 culturable strains and 125 clones from the 16S rRNA gene
libraries. In conventional tillage, there were 38% Proteobacteria,
22% Actinobacteria, 33% Firmicutes, 5% Bacteroidetes,
and 2% Acidobacteria, whereas with no-tillage management
there were 63% Proteobacteria, 24% Actinobacteria, 6% Firmicutes,
and 8% Bacteroidetes as estimated using the culturedependent
method
during the four stages of rice cultivation.
Principal coordinates analysis was used to cluster the bacterial
communities along axes of maximal variance. The different
growth stages of rice appeared to influence the rhizosphere
bacterial profile for both cultivation practices. Novel
clones with low similarities (89–97%) to Actinobacteria and
Streptomyces were retrieved from both rice fields by screening
the 16S rRNA gene libraries using actinobacterial- and
streptomycete-specific primers. By comparing the actinobacterial
community retrieved by culture-dependent and
molecular methods, it was clear that a more comprehensive
assessment of microbial diversity in the rice rhizosphere can
be obtained using a combination of both techniques than
by using either method alone. We also succeeded in culturing
a number of bacteria that were previously described as
unculturable. These were in a phylogenetically deep lineage when compared with related cultivable genera.
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Agriculture.2025; 15(11): 1145. CrossRef - Unusual Morphological Changes of a Novel Wrinkled Bacterium Isolated from the Rice Rhizosphere Under Nutrient Stress
Young Ryun Chung, Jung Eun Lee, Zubair Aslam, Eu Jin Chung, Kwang Hee Lee, Byung Ho Kang, Ajmal Khan, Sarbjeet Niraula, Woo-Suk Chang
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Chemical and Biological Technologies in Agriculture.2024;[Epub] CrossRef - Soil and Phytomicrobiome for Plant Disease Suppression and Management under Climate Change: A Review
Wen Chen, Dixi Modi, Adeline Picot
Plants.2023; 12(14): 2736. CrossRef - Microbial Community Shifts with Soil Properties and Enzyme Activities in Inter-/Mono-Cropping Systems in Response to Tillage
Peina Lu, Cai Zhao, Wen Yin, Falong Hu, Zhilong Fan, Aizhong Yu, Hong Fan
Agronomy.2023; 13(11): 2707. CrossRef - Long-term agro-management strategies shape soil bacterial community structure in dryland wheat systems
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Scientific Reports.2023;[Epub] CrossRef - Microbial Inoculations Promoted the Rice Plant Growth by Regulating the Root-Zone Bacterial Community Composition and Potential Function
Yifeng Gui, Caihong Gu, Xian Xiao, Yuexiang Gao, Yuan Zhao
Journal of Soil Science and Plant Nutrition.2023; 23(4): 5222. CrossRef - Study of Rhizosphere Microbial Community Structures of Asian Wild and Cultivated Rice Showed That Cultivated Rice Had Decreased and Enriched Some Functional Microorganisms in the Process of Domestication
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- Diversity of Cyanobacterial Species and Phylotypes in Biofilms from the Littoral Zone of Lake Baikal
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Ekaterina G. Sorokovikova , Olga I. Belykh , Anna S. Gladkikh , Oleg V. Kotsar , Irina V. Tikhonova , Oleg A. Timoshkin , Valentina V. Parfenova
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J. Microbiol. 2013;51(6):757-765. Published online December 19, 2013
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DOI: https://doi.org/10.1007/s12275-013-3240-4
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328
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12
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Abstract
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The majority of naturally occurring biofilms contain numerous
microorganisms that have not yet been cultured.
Additionally, there is little information available regarding
the genetic structure and species diversity of these communities.
Therefore, we characterised the species diversity, structure
and metagenome of biofilms grown on stones and
steel plates in the littoral zone of Lake Baikal (East Siberia,
Russia) by applying three different approaches. First, light
microscopy enabled identification of the species diversity of
biofilm-forming cyanobacteria on different substrates with
the dominance of Rivularia rufescens, Tolypothrix limbata,
Chamaesiphon fuscus, Сh. subglobosus, and Heteroleibleinia
pusilla. Additionally, scanning electron microscopy was used
to show the spatial structure of biofilms. Finally, sequence
analysis of 30,660 16S rRNA clones indicated a high diversity
within the biofilm communities, with the majority of the
microbes being closely related to Cyanobacteria (8–46% sequences),
Proteobacteria (14–43%), and Bacteroidetes (10–
41%). Rivularia sp., Pseudanabaena sp., and Chamaesiphon
spp. were the dominant cyanobacterial phylotypes.
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- Genomic Insights into the Bactericidal and Fungicidal Potential of Bacillus mycoides b12.3 Isolated in the Soil of Olkhon Island in Lake Baikal, Russia
Maria N. Romanenko, Anton E. Shikov, Iuliia A. Savina, Fedor M. Shmatov, Anton A. Nizhnikov, Kirill S. Antonets
Microorganisms.2024; 12(12): 2450. CrossRef - Time-series metagenomics reveals changing protistan ecology of a temperate dimictic lake
Arianna I. Krinos, Robert M. Bowers, Robin R. Rohwer, Katherine D. McMahon, Tanja Woyke, Frederik Schulz
Microbiome.2024;[Epub] CrossRef - Impact of meltwater flow intensity on the spatiotemporal heterogeneity of microbial mats in the McMurdo Dry Valleys, Antarctica
A Zoumplis, B Kolody, D Kaul, H Zheng, P Venepally, D M McKnight, C Takacs-Vesbach, A DeVries, A E Allen
ISME Communications.2023;[Epub] CrossRef - Water Quality, Toxicity and Diversity of Planktonic and Benthic Cyanobacteria in Pristine Ancient Lake Khubsugul (Hövsgöl), Mongolia
Olga I. Belykh, Ekaterina G. Sorokovikova, Irina V. Tomberg, Galina A. Fedorova, Anton V. Kuzmin, Andrey Yu. Krasnopeev, Maria Yu. Suslova, Sergey A. Potapov, Tatiana I. Belykh, Jadambaa Norovsuren, Agnia D. Galachyants, Irina V. Tikhonova
Toxins.2023; 15(3): 213. CrossRef - Diversity of Aerobic Anoxygenic Phototrophs and Rhodopsin-Containing Bacteria in the Surface Microlayer, Water Column and Epilithic Biofilms of Lake Baikal
Agnia Dmitrievna Galachyants, Andrey Yurjevich Krasnopeev, Galina Vladimirovna Podlesnaya, Sergey Anatoljevich Potapov, Elena Viktorovna Sukhanova, Irina Vasiljevna Tikhonova, Ekaterina Andreevna Zimens, Marsel Rasimovich Kabilov, Natalia Albertovna Zhuch
Microorganisms.2021; 9(4): 842. CrossRef - First data on cyanobacterial biodiversity in benthic biofilms during mass mortality of endemic sponges in Lake Baikal
Ekaterina Sorokovikova, Olga Belykh, Andrey Krasnopeev, Sergey Potapov, Irina Tikhonova, Igor Khanaev, Marsel Kabilov, Olga Baturina, Galina Podlesnaya, Oleg Timoshkin
Journal of Great Lakes Research.2020; 46(1): 75. CrossRef - Microbial bloom formation in a high pH spent nuclear fuel pond
Lynn Foster, Christopher Boothman, Sharon Ruiz-Lopez, Genevieve Boshoff, Peter Jenkinson, David Sigee, Jon K. Pittman, Katherine Morris, Jonathan R. Lloyd
Science of The Total Environment.2020; 720: 137515. CrossRef - Variation of sponge-inhabiting infauna with the state of health of the sponge Lubomirskia baikalensis (Pallas, 1776) in Lake Baikal
Yulia Zvereva, Olga Medvezhonkova, Tatyana Naumova, Natalia Sheveleva, Anton Lukhnev, Ekaterina Sorokovikova, Taisia Evstigneeva, Oleg Timoshkin
Limnology.2019; 20(3): 267. CrossRef - Diazotrophic cyanobacteria signatures and their relationship to hydrographic conditions in the Gulf of Gabes, Tunisia
Zaher Drira, Dorra Chaari, Asma Hamza, Malika Bel Hassen, Marc Pagano, Habib Ayadi
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David J. Van Horn, Caitlin R. Wolf, Daniel R. Colman, Xiaoben Jiang, Tyler J. Kohler, Diane M. McKnight, Lee F. Stanish, Terrill Yazzie, Cristina D. Takacs-Vesbach, Max Häggblom
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Sudipta Kumar Das
Feddes Repertorium.2015; 126(1-2): 22. CrossRef - Distribution and Ecology of Cyanobacteria in the Rocky Littoral of an English Lake District Water Body, Devoke Water
Allan Pentecost
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- The Proportion of Non-Aflatoxigenic Strains of the Aspergillus flavus/oryzae Complex from Meju by Analyses of the Aflatoxin Biosynthetic Genes
-
Seung-Beom Hong , Mina Lee , Dae-Ho Kim , Soo-Hyun Chung , Hyeon-Dong Shin , Robert A. Samson
-
J. Microbiol. 2013;51(6):766-772. Published online December 19, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3128-3
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339
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14
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Abstract
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Strains of the Aspergillus flavus/oryzae complex are frequently
isolated from meju, a fermented soybean product, that is used
as the starting material for ganjang (soy sauce) and doenjang
(soybean paste) production. In this study, we examined the
aflatoxin producing capacity of A. flavus/oryzae strains isolated
from meju. 192 strains of A. flavus/oryzae were isolated
from more than 100 meju samples collected from diverse
regions of Korea from 2008 to 2011, and the norB-cypA,
omtA, and aflR genes in the aflatoxin biosynthesis gene
cluster were analyzed. We found that 178 strains (92.7%)
belonged to non-aflatoxigenic group (Type I of norB-cypA,
IB-L-B-, IC-AO, or IA-L-B- of omtA, and AO type of aflR),
and 14 strains (7.3%) belonged to aflatoxin-producible group
(Type II of norB-cypA, IC-L-B+/B- or IC-L-B+ of omtA, and
AF type of aflR). Only 7 strains (3.6%) in the aflatoxin-producible
group produced aflatoxins on Czapek yeast-extract
medium. The aflatoxin-producing capability of A. flavus/
oryzae strains from other sources in Korea were also investigated,
and 92.9% (52/56) strains from air, 93.9% (31/33)
strains from rice straw, 91.7% (11/12) strains from soybean,
81.3% (13/16) strains from corn, 82% (41/50) strains from
peanut, and 73.2% (41/56) strains from arable soil were included
in the non-aflatoxigenic group. The proportion of
non-aflatoxigenicity of meju strains was similar to that of
strains from soybean, air and rice straw, all of which have
an effect on the fermentation of meju. The data suggest that
meju does not have a preference for non-aflatoxigenic or aflatoxin-producible strains of A. flavus/oryzae from the
environment of meju. The non-aflatoxigenic meju strains
are proposed to be named A. oryzae, while the meju strains
that can produce aflatoxins should be referred to A. flavus
in this study.
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Dong-Hyun Kim, Dong-Chan Kim, Donggun Seo, Ki-Tae Kim, Sang-Han Lee, Seung-Beom Hong
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Jong-Gyu Kim, Jeong-Yeong Park
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Tadahiro Suzuki, Yumiko Iwahashi
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Hyeonheui Ham, Sosoo Kim, Min-Hee Kim, Soohyung Lee, Sung Kee Hong, Jae-Gee Ryu, Theresa Lee
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The Korean Journal of Mycology.2015;[Epub] CrossRef - High sequence variations in the region containing genes encoding a cellular morphogenesis protein and the repressor of sexual development help to reveal origins of Aspergillus oryzae
Perng-Kuang Chang, Leslie L. Scharfenstein, Cesar D. Solorzano, Hamed K. Abbas, Sui-Sheng T. Hua, Walker A. Jones, Robert M. Zablotowicz
International Journal of Food Microbiology.2015; 200: 66. CrossRef - The potential hazards of Aspergillus sp. in foods and feeds, and the role of biological treatment: A review
Sheikh Imranudin Sheikh-Ali, Akil Ahmad, Siti-Hamidah Mohd-Setapar, Zainul Akmal Zakaria, Norfahana Abdul-Talib, Aidee Kamal Khamis, Md Enamul Hoque
Journal of Microbiology.2014; 52(10): 807. CrossRef - Diversity, Saccharification Capacity, and Toxigenicity Analyses of Fungal Isolates in Nuruk
Min Sik Kim, Sinil Kim, Byeong-Seok Ha, Hye-Young Park, Seong-Yeol BaeK, Soo-Hwan Yeo, Hyeon-Su Ro
The Korean Journal of Mycology.2014; 42(3): 191. CrossRef
- Enhanced Production of Biomass and Lipids by Supplying CO2 in Marine Microalga Dunaliella sp.
-
Hancheol Jeon , Yew Lee , Kwang Suk Chang , Choul-Gyun Lee , EonSeon Jin
-
J. Microbiol. 2013;51(6):773-776. Published online December 19, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3256-9
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321
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7
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Abstract
PDF
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Non-food-based biofuel feedstocks are in high demand
worldwide. Among the various feedstocks, microalgae are
the most promising feedstock for mitigating atmospheric
CO2 and producing biodiesel. In this study, various concentrations
of CO2, from 0.03 to 12%, were used to investigate
their effect on the cell growth, biomass and lipid production
and fatty acid composition of Dunaliella sp. in a
closed photobioreactor. The results showed that the highest
biomass and total lipids, 521 mg/L/d and 40 mg/L/d, respectively,
were produced with 5% CO2 aeration during the
logarithmic growth phase. The oleic acid (18:1n9c) and elaidic
acid (18:1n9t) contents were increased approximately
two fold. The physiological responses of Dunaliella sp. at
10% CO2 were similar to those at 5% CO2. Therefore, the
present results suggest that 5–10% is a suitable CO2 concentration
range for Dunaliella sp. growth to mitigate atmospheric
CO2 and increase biofuel production.
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Citations
Citations to this article as recorded by

- Synergistic impact of elevated CO2 and photo-bioreactor illumination surface area on marine microalgal biomass and bio-chemicals production
Venkatesan Ajithkumar, V. Isaimozhi, Bhavika Mehta, Kirti Singhal, Shreya Sadukha, Koustav Biswas, Arup Ghosh, Ramalingam Dineshkumar
Algal Research.2025; 91: 104156. CrossRef - Stabilized and Immobilized Carbonic Anhydrase on Electrospun Nanofibers for Enzymatic CO2 Conversion and Utilization in Expedited Microalgal Growth
Seung-Hyun Jun, Jusang Yang, Hancheol Jeon, Han Sol Kim, Seung Pil Pack, EonSeon Jin, Jungbae Kim
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Roya Moghimifam, Vahid Niknam, Hassan Ebrahimzadeh, Mohammad Amin Hejazi
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S. A. Razzak
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Eun Jae Kim, Woongsic Jung, Suyoun Lim, Sanghee Kim, Han-Gu Choi, Se Jong Han
Applied Biochemistry and Biotechnology.2019; 188(2): 326. CrossRef - Microalgal Cultivation in Secondary Effluent: Recent Developments and Future Work
Junping Lv, Jia Feng, Qi Liu, Shulian Xie
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Elisa Luengo, Santiago Condón-Abanto, Ignacio Álvarez, Javier Raso
The Journal of Membrane Biology.2014; 247(12): 1269. CrossRef
- Lactobacillus paracasei subsp. paracasei LC01 Positively Modulates Intestinal Microflora in Healthy Young Adults
-
Hao Zhang , Jing Sun , Xianting Liu , Chuan Hong , Yuanbo Zhu , Aiping Liu , Siqi Li , Huiyuan Guo , Fazheng Ren
-
J. Microbiol. 2013;51(6):777-782. Published online December 19, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3279-2
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279
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21
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Abstract
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Lactobacillus paracasei subsp. paracasei LC01 (LC01) can
tolerate intestinal stresses and has antioxidant activity. To
evaluate the effect of the bacterium on human intestinal microflora,
a randomized, double-blind, placebo-controlled
human trial was carried out. Fifty-two healthy adult volunteers
were randomized equally to two groups. One group
consumed 12% (wt/vol) skimmed milk supplemented with
1010 CFU of LC01 each day for the 4-week treatment period,
and then consumed placebo in the next treatment period,
separated by a 2-week washout. The other group followed
the reverse order. Group-specific real-time PCR and biochemical
analyses was used to determine the intestinal bacterial
composition of fecal samples collected at the end of
every period, and the concentration of short-chain fatty acids
and ammonia. A significant inhibition in fecal Escherichia
coli and increase in Lactobacillus, Bifidobacterium, and Roseburia
intestinalis were observed after consumption of LC01.
Acetic acid and butyric acid were significantly higher in the
probiotic stage and fecal ammonia was significantly lower.
The results indicated a modulation effect of LC01 on the
intestinal microflora of young adults, suggesting a beneficial
effect on bowel health. LC01 may have potential value as a
probiotic.
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Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef - Lactobacillus casei LC01 Regulates Intestinal Epithelial Permeability through miR-144 Targeting of OCLN and ZO1
Qiuke Hou, Yongquan Huang, Yan Wang, Liu Liao, Zhaoyang Zhu, Wenjie Zhang, Yongshang Liu, Peiwu Li, Xinlin Chen, Fengbin Liu
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Roseburia
Spp.: A Marker of Health?
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Ruby Yadav, Pratyoosh Shukla
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Junpei Nakase, Yuuichi Ukawa, Syoji Takemoto, Takayoshi Kubo, Yuko M Sagesaka, Ayako Aoki-Yoshida, Mamoru Totsuka
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Esther Nova, Fátima Pérez de Heredia, Sonia Gómez‐Martínez, Ascensión Marcos
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Gregor Reid
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Muriel Derrien, Johan E.T. van Hylckama Vlieg
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Joo Yeon Kang, Do Kyung Lee, Nam Joo Ha, Hea Soon Shin
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- Influence of Acetobacter pasteurianus SKU1108 aspS Gene Expression on Escherichia coli Morphology
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Kannipa Tasanapak , Uraiwan Masud-Tippayasak , Kazunobu Matsushita , Wichien Yongmanitchai , Gunjana Theeragool
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J. Microbiol. 2013;51(6):783-790. Published online December 19, 2013
-
DOI: https://doi.org/10.1007/s12275-013-2619-6
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301
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3
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Abstract
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The aspS gene encoding Aspartyl-tRNA synthetase (AspRS)
from a thermotolerant acetic acid bacterium, Acetobacter
pasteurianus SKU1108, has been cloned and characterized.
The open reading frame (ORF) of the aspS gene consists of
1,788 bp, encoding 595 amino acid residues. The highly
conserved Gly-Val-Asp-Arg ATP binding motif (motif 3) is
located at the position 537-540 in the C-terminus. Deletion
analysis of the aspS gene upstream region suggested that
the promoter is around 173 bp upstream from the ATG initiation
codon. Interestingly, transformation with the plasmids
pGEM-T138, pUC138, and pCM138 synthesizing 138
amino acid C-terminal fragments of AspRS, that carry the
ATP binding domain, caused E. coli cell lengthening at 37 and
42°C. Moreover, E. coli harboring pUC595 (synthesizing all
595 amino acids) and a disordered aspS gene in pGEM-T138
had normal rod shapes. The normal rod shape was observed
in E. coli harboring pD539V following site-directed mutagenesis
of the ATP binding domain. We propose that overproduction
of truncated C-terminal peptides of AspRS may
cause sequestration of intracellular ATP in E. coli, leaving
less ATP for cell division or shaping cell morphology.
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Citations
Citations to this article as recorded by

- Response of the microbiome–gut–brain axis in Drosophila to amino acid deficit
Boram Kim, Makoto I. Kanai, Yangkyun Oh, Minsoo Kyung, Eun-Kyoung Kim, In-Hwan Jang, Ji-Hoon Lee, Sang-Gyu Kim, Greg S. B. Suh, Won-Jae Lee
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De-Quan Zhu, Fei Liu, Yu Sun, Li-Mei Yang, Li Xin, Xiang-Chen Meng, Yung-Fu Chang
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Natsaran Saichana, Kazunobu Matsushita, Osao Adachi, Ivo Frébort, Jitka Frebortova
Biotechnology Advances.2015; 33(6): 1260. CrossRef
- Trans-Membrane Transport of n-Octadecane by Pseudomonas sp. DG17
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Fei Hua , Hong Qi Wang , Yi Li , Yi Cun Zhao
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J. Microbiol. 2013;51(6):791-799. Published online December 19, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3259-6
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275
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17
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Abstract
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The trans-membrane transport of hydrocarbons is an important
and complex aspect of the process of biodegradation
of hydrocarbons by microorganisms. The mechanism of
transport of 14C n-octadecane by Pseudomonas sp. DG17,
an alkane-degrading bacterium, was studied by the addition
of ATP inhibitors and different substrate concentrations.
When the concentration of n-octadecane was higher than
4.54 μmol/L, the transport of 14C n-octadecane was driven
by a facilitated passive mechanism following the intra/extra
substrate concentration gradient. However, when the cells
were grown with a low concentration of the substrate, the
cellular accumulation of n-octadecane, an energy-dependent
process, was dramatically decreased by the presence of ATP
inhibitors, and n-octadecane accumulation continually increased
against its concentration gradient. Furthermore, the
presence of non-labeled alkanes blocked 14C n-octadecane
transport only in the induced cells, and the trans-membrane
transport of n-octadecane was specific with an apparent
dissociation constant Kt of 11.27 μmol/L and Vmax of 0.96
μmol/min/mg protein. The results indicated that the transmembrane
transport of n-octadecane by Pseudomonas sp.
DG17 was related to the substrate concentration and ATP.
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Citations
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Journal of Hazardous Materials.2023; 441: 129963. CrossRef - Bioaugmentation of diesel-contaminated soil with Pseudomonas sp. DTF1
H. Yang, G. Kim, K.-S. Cho
International Journal of Environmental Science and Technology.2023; 20(11): 12499. CrossRef - Potential Egyptian bacterial consortium for oil spill treatment: A laboratory simulation
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- A Putative APSES Transcription Factor Is Necessary for Normal Growth and Development of Aspergillus nidulans
-
Ji-Yeon Lee , Lee-Han Kim , Ha-Eun Kim , Jae-Sin Park , Kap-Hoon Han , Dong-Min Han
-
J. Microbiol. 2013;51(6):800-806. Published online December 19, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3100-2
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312
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14
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Abstract
PDF
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The nsdD gene encoding a GATA type transcription factor
positively controls sexual development in Aspergillus nidulans.
According to microarray data, 20 genes that were upregulated
by deleting nsdD during various life cycle stages were
randomly selected and deleted for functional analysis. None
of the mutants showed apparent changes in growth or development
compared with those of the wild-type except the
AN3154 gene that encodes a putative APSES transcription
factor and is an ortholog of Saccharomyces cerevisiae swi4.
Deleting AN3154 resulted in retarded growth and development,
and the gene was named rgdA (retared growth and
development). The rgdA deletion mutant developed a reduced
number of conidia even under favorable conditions for asexual
development. The retarded growth and development was
partially suppressed by the veA1 mutation. The conidial heads
of the mutant aborted, showing reduced and irregular shaped
phialides. Fruiting body development was delayed compared
with that in the wild-type. The mutant did not respond to
various nutritional or environmental factors that affected the
development patterns. The rgdA gene was expressed at low
levels throughout the life cycle and was not significantly affected
by several regulators of sexual and asexual development
such as nsdD, veA, stuA, or brlA. However, the rgdA gene
affected brlA and abaA expression, which function as key
regulators of asexual sporulation, suggesting that rgdA functions
upstream of those genes.
-
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Xiao Li, Qianqian Zhang, Qili Liu, Xiaobin Xu, Jinzhu Li, Dandan Zhu, Yuanyuan Zong, Huali Xue, Yang Bi
Postharvest Biology and Technology.2025; 227: 113628. CrossRef - Putative APSES family transcription factor mbp1 plays an essential role in regulating cell wall synthesis in the agaricomycete Pleurotus ostreatus
Hayase Kojima, Moriyuki Kawauchi, Yuitsu Otsuka, Kim Schiphof, Kenya Tsuji, Akira Yoshimi, Chihiro Tanaka, Shigekazu Yano, Takehito Nakazawa, Yoichi Honda
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Ye-Eun Son, Jae-Hyuk Yu, Hee-Soo Park
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Yong-Ho Choi, Sang-Cheol Jun, Min-Woo Lee, Jae-Hyuk Yu, Kwang-Soo Shin
International Journal of Molecular Sciences.2021; 22(7): 3777. CrossRef - The Putative APSES Transcription Factor RgdA Governs Growth, Development, Toxigenesis, and Virulence in Aspergillus fumigatus
Sang-Cheol Jun, Yong-Ho Choi, Min-Woo Lee, Jae-Hyuk Yu, Kwang-Soo Shin, Aaron P. Mitchell
mSphere.2020;[Epub] CrossRef - Analogous and Diverse Functions of APSES-Type Transcription Factors in the Morphogenesis of the Entomopathogenic Fungus Metarhizium rileyi
Caiyan Xin, Jinping Zhang, Siji Nian, Guangxi Wang, Zhongkang Wang, Zhangyong Song, Guangwei Ren, Ning-Yi Zhou
Applied and Environmental Microbiology.2020;[Epub] CrossRef - Distribution, evolution and expression ofGATA-TFsprovide new insights into their functions in light response and fruiting body development ofTolypocladium guangdongense
Chenghua Zhang, Gangzheng Wang, Wangqiu Deng, Taihui Li
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Larissa V G Longo, Stephanie C Ray, Rosana Puccia, Chad A Rappleye
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Guangshan Yao, Feng Zhang, Xinyi Nie, Xiuna Wang, Jun Yuan, Zhenhong Zhuang, Shihua Wang
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Lucas Nojosa Oliveira, Luciana Casaletti, Sônia Nair Báo, Clayton Luiz Borges, Patrícia de Sousa Lima, Célia Maria de Almeida Soares
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Mohammed A. Abdo Elgabbar, Kap-Hoon Han
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Eun-Hye Kang, Eun-Jung Song, Jun Ho Kook, Hwan-Hee Lee, Bo-Ri Jeong, Hee-Moon Park
Mycobiology.2015; 43(1): 31. CrossRef - FgFlbD regulates hyphal differentiation required for sexual and asexual reproduction in the ascomycete fungus Fusarium graminearum
Hokyoung Son, Myung-Gu Kim, Suhn-Kee Chae, Yin-Won Lee
Journal of Microbiology.2014; 52(11): 930. CrossRef - Transcriptional regulation of fksA, a β-1,3-glucan synthase gene, by the APSES protein StuA during Aspergillus nidulans development
Bum-Chan Park, Yun-Hee Park, Soohyun Yi, Yu Kyung Choi, Eun-Hye Kang, Hee-Moon Park
Journal of Microbiology.2014; 52(11): 940. CrossRef
- Isolation and Functional Characterization of a Delta 6-Desaturase Gene from the Pike Eel (Muraenesox cinereus)
-
Sun Hee Kim , Kyung Hee Roh , Jung-Bong Kim , Kwang-Soo Kim , Nam Shin Kim , Hyun Uk Kim , Kyeong-Ryeol Lee , Jong-Sug Park , Jong-Bum Kim
-
J. Microbiol. 2013;51(6):807-813. Published online October 5, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3144-3
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319
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9
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Abstract
PDF
-
Stearidonic acid (STA; 18:4n-3) and γ-linolenic acid (GLA;
18:3n-6) are significant intermediates in the biosynthetic pathway
for the very-long-chain polyunsaturated fatty acids of
eicosapentaenoic acid (EPA; 20:5n-3) and arachidonic acid
(ARA; 20:4n-6), respectively. To develop a sustainable system
for the production of dietary polyunsaturated fatty acids,
we focused on the action of the enzyme delta 6-desaturase
(D6DES) on the essential acids, linoleic acid (LA; 18:2n-6)
and α-linolenic acid (ALA; 18:3n-3). A 1,335-bp full-length
cDNA encoding D6DES (McD6DES) was cloned from Muraenesox
cinereus using degenerate PCR and RACE-PCR
methods
. To investigate the enzymatic activity of McD6DES
in the production of n-6 and n-3 fatty acids, a recombinant
plasmid expressing McD6DES (pYES-McD6DES) was transformed
into and expressed in Saccharomyces cerevisiae. The
exogenously expressed McD6DES produced GLA and STA
at conversion rates of 14.2% and 45.9%, respectively, from
the exogenous LA and ALA substrates. These results indicate
that McD6DES is essentially a delta 6-desaturase involved
in very-long-chain polyunsaturated fatty acid synthesis.
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Research Support, U.S. Gov't, Non-P.H.S.
- Structure and Function of the Mating-type Locus in the Homothallic Ascomycete, Didymella zeae-maydis
-
Sung-Hwan Yun , Olen C. Yoder , B. Gillian Turgeon
-
J. Microbiol. 2013;51(6):814-820. Published online December 19, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3465-2
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290
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12
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Abstract
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Homothallic Didymella zeae-maydis undergoes sexual reproduction
by selfing. Sequence analysis of the mating type
(MAT) locus from this fungus revealed that MAT carries
both MAT1-1-1 and MAT1-2-1 genes found in heterothallic
Dothideomycetes, separated by ~1.0 kb of noncoding DNA.
To understand the mechanistic basis of homothallism in D.
zeae-maydis, each of the MAT genes was deleted and the effects
on selfing and on ability to cross in a heterothallic manner
were determined. The strain carrying an intact MAT1-1-1
but defective MAT1-2-1 gene (MAT1-1-1;ΔMAT1-2-1) was
self-sterile, however strains carrying an intact MAT1-2-1 but
defective MAT1-1-1 gene (ΔMAT1-1-1;MAT1-2-1), when
selfed, showed delayed production of a few ascospores.
Attempts to cross the two MAT deletion strains yielded fewer
ΔMAT1-1-1;MAT1-2-1 than MAT1-1-1;ΔMAT1-2-1 progeny
and very few ascospores overall compared to WT selfs. This
study demonstrates that, as in the other homothallic Dothideomycetes,
both MAT genes are required for full fertility,
but that, in contrast to other cases, the presence of a single
MAT1-2-1 gene can induce homothallism, albeit inefficiently,
in D. zeae-maydis.
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Citations
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- Structure and number of mating pheromone genes is closely linked to sexual reproductive strategy in Huntiella
Andi M. Wilson, Michael J. Wingfield, Brenda D. Wingfield
BMC Genomics.2023;[Epub] CrossRef - The Role of Chromatin and Transcriptional Control in the Formation of Sexual Fruiting Bodies in Fungi
Minou Nowrousian
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Lucia Ramos Romero, Dagmar Tacke, Birger Koopmann, Andreas von Tiedemann
Pathogens.2021; 10(9): 1216. CrossRef - Integrative Activity of Mating Loci, Environmentally Responsive Genes, and Secondary Metabolism Pathways during Sexual Development of Chaetomium globosum
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Fungal Genetics and Biology.2017; 109: 16. CrossRef - Genetic Dissection of Sexual Reproduction in a Primary Homothallic Basidiomycete
Márcia David-Palma, José Paulo Sampaio, Paula Gonçalves, Joseph Heitman
PLOS Genetics.2016; 12(6): e1006110. CrossRef - Mating-Type Gene Structure and Spatial Distribution ofDidymella tanacetiin Pyrethrum Fields
Tamieka L. Pearce, Jason B. Scott, Frank S. Hay, Sarah J. Pethybridge
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Fungal Sex: The
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Richard J. Bennett, B. Gillian Turgeon, Joseph Heitman
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Research Support, Non-U.S. Gov'ts
- Azole-synergistic Anti-Candidal Activity of Altenusin, a Biphenyl Metabolite of the Endophytic Fungus Alternaria alternata Isolated from Terminalia chebula Retz.
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Jatuporn Phaopongthai , Suthep Wiyakrutta , Duangdeun Meksuriyen , Nongluksna Sriubolmas , Khanit Suwanborirux
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J. Microbiol. 2013;51(6):821-828. Published online December 19, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3189-3
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281
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0
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23
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Abstract
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In this study, a tropical endophytic fungus, Alternaria alternata
Tche-153 was isolated from a Thai medicinal plant
Terminalia chebula Rezt. The ethyl acetate extract prepared
from the fermentation broth exhibited significant ketoconazole-
synergistic activity against Candida albicans. Bioassaydirected
fractionation of the ethyl acetate extract led to the
isolation of altenusin (1), isoochracinic acid (2), and altenuic
acid (3) together with 2,5-dimethyl-7-hydroxychromone
(4). Using the disc diffusion method and the microdilution
chequerboard technique, only altenusin (1) in combination
with each of three azole drugs, ketoconazole, fluconazole or
itraconazole at their low sub-inhibitory concentrations exhibited
potent synergistic activity against C. albicans with the
fractional inhibitory concentration index range of 0.078 to
0.188. This first discovery of altenusin (1) as a new azole-synergistic
prototype possessing a biphenyl structure is of significance
for further development of new azole-synergists
to treat invasive candidiasis.
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- Reduction of Organic and Inorganic Selenium Compounds by the Edible Medicinal Basidiomycete Lentinula edodes and the Accumulation of Elemental Selenium Nanoparticles in Its Mycelium
-
Elena Vetchinkina , Ekaterina Loshchinina , Viktor Kursky , Valentina Nikitina
-
J. Microbiol. 2013;51(6):829-835. Published online December 19, 2013
-
DOI: https://doi.org/10.1007/s12275-013-2689-5
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354
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59
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Abstract
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We report for the first time that the medicinal basidiomycete
Lentinula edodes can reduce selenium from inorganic sodium
selenite (SeIV) and the organoselenium compound 1,5-
diphenyl-3-selenopentanedione-1,5 (DAPS-25) to the elemental
state, forming spherical nanoparticles. Submerged
cultivation of the fungus with sodium selenite or with DAPS-
25 produced an intense red coloration of L. edodes mycelial
hyphae, indicating accumulation of elemental selenium (Se0)
in a red modification. Several methods, including transmission
electron microscopy (TEM), electron energy loss
spectroscopy (EELS), and X-ray fluorescence, were used to
show that red Se0 accumulated intracellularly in the fungal
hyphae as electron-dense nanoparticles with a diameter of
180.51±16.82 nm. Under designated cultivation conditions,
shiitake did not reduce selenium from sodium selenate
(SeVI).
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- Cyclic Dipeptides from Lactic Acid Bacteria Inhibit Proliferation of the Influenza A Virus
-
Min-Kyu Kwak , Rui Liu , Jun-Oh Kwon , Min-Kyu Kim , Andrew HyoungJin Kim , Sa-Ouk Kang
-
J. Microbiol. 2013;51(6):836-843. Published online December 19, 2013
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DOI: https://doi.org/10.1007/s12275-013-3521-y
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Abstract
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We isolated Lactobacillus plantarum LBP-K10 from the traditional Korean fermented food kimchi. When organic acids were removed, the culture filtrate of this isolate showed high antiviral activity (measured using a plaque-forming assay) against the influenza A (H3N2) virus. Two fractions that were active against influenza A virus were purified from the culture filtrate using a C18 column with high-performance liquid chromatography. These active fractions were crystallized and identified to be the cyclic dipeptides cis-cyclo (L-Leu-L-Pro) and cis-cyclo(L-Phe-L-Pro) using gas chromatography-mass spectrometry; this identification was confirmed by X-ray crystallography. These cyclic dipeptides were identified in the culture filtrate of other lactic acid bacteria, including Lactobacillus spp., Leuconostoc spp., Weissella spp.,
and Lactococcus lactis.
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- Inhibitory Effect of Chlorophyllin on the Propionibacterium acnes-Induced Chemokine Expression
-
Mi-Sun Kang , Jin-Hee Kim , Boo-Ahn Shin , Hyun-Chul Lee , Youn-Shin Kim , Hae-Soon Lim , Jong-Suk Oh
-
J. Microbiol. 2013;51(6):844-849. Published online December 19, 2013
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DOI: https://doi.org/10.1007/s12275-013-3015-y
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282
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0
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10
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Abstract
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Chlorophyllin (CHL), a chlorophyll-derivative, exhibits several
beneficial properties, including antibacterial, antioxidant,
and anticancer activities. However, its antibacterial and
anti-inflammatory activities against Propionibacterium acnes
have not been described. The antibacterial activity of this compound
was evaluated in vitro using the broth microdilution
method
. CHL had an inhibitory effect on the growth of P.
acnes (MIC = 100 μM). In a real-time reverse transcriptionpolymerase
chain reaction and an enzyme-linked immunosorbent
assay, CHL significantly decreased interleukin-8
(IL-8) and monocyte chemoattractant protein-1 (MCP-1)
production in a dose-dependent manner, decreasing both
mRNA and protein levels for these chemokines in THP-1
cells indicating the anti-inflammatory effects of it. To investigate
the molecular mechanisms underlying the anti-inflammatory
properties of CHL in THP-1 cells stimulated
by P. acnes, we used western blotting to analyze the effect of
CHL on activation of the nuclear factor (NF)-κB. CHL inhibited
P. acnes-induced IL-8 and MCP-1 production via
blockade of NF-κB activation in THP-1 cells. Therefore,
based on these results, we suggest that CHL is a useful agent
to control the growth of P. acnes involved in acne inflammation
and prevent acne.
-
Citations
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- Endophytic Fungal Pre-treatments of Seeds Alleviates Salinity Stress ffects in Soybean Plants
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Ramalingam Radhakrishnan , Abdul Latif Khan , In-Jung Lee
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J. Microbiol. 2013;51(6):850-857. Published online December 19, 2013
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DOI: https://doi.org/10.1007/s12275-013-3168-8
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315
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Abstract
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In the present study, four endophytic fungi (GM-1, GM-2,
GM-3, and GM-4) were tested for their ability to improve
soybean plant growth under salinity stress conditions. The
seed germination and plant growth were higher in seeds pretreated
with endophytic fungal cultures than their controls.
The positive influence of fungi on plant growth was supported
by gibberellins analysis of culture filtrate (CF), which
showed wide diversity and various concentrations of GAs.
Specifically, GA4, GA7, GA8, GA9, GA12, and GA20 were
found in fungal CFs. Under salinity stress conditions, GM-1
significantly enhanced the length and fresh weight of soybean
plants relative to other fungal treatments. GM-1 effectively
mitigated the adverse effects of salinity by limiting
lipid peroxidation and accumulating protein content. GM-2,
GM-3, and GM-4 also counteracted the salinity induced
oxidative stress in soybean plants through reduction of lipid
peroxidation and enhancement of protein content, maintaining
the length and fresh weight of shoots. The activities
of the antioxidant enzymes catalase, superoxide dismutase
and peroxidase were inhibited in salinity exposed plants,
while GM-1 significantly enhanced these antioxidant enzyme
activities in plants under salt stress. GM-1 treatment also
showed lower levels of abscisic acid and elevated levels of
salicylic acid in plants under salinity stress. Hence, GM-1 was
identified as Fusarium verticillioides (teleomorph Gibberella
moniliformis) isolate RK01 based on its DNA sequence homology.
These results suggest that endophytic fungal (F.
verticillioides) pre-treatment of soybean seeds would be an
effective method to promote soybean plant growth under
salinity stress conditions.
-
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- Evaluation of Bakanae Disease Progression Caused by Fusarium fujikuroi in Oryza sativa L.
-
In Sun Hwang , Woo-Ri Kang , Duk-Ju Hwang , Shin-Chul Bae , Sung-Hwan Yun , Il-Pyung Ahn
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J. Microbiol. 2013;51(6):858-865. Published online December 19, 2013
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DOI: https://doi.org/10.1007/s12275-013-3472-3
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338
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Abstract
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Bakanae disease caused by Fusarium fujikuroi is an important
fungal disease in rice. Among the seven strains isolated from
symptomatic rice grains in this study, one strain, FfB14,
triggered severe root growth inhibition and decay in the
crown and root of rice seedlings. The remaining six strains
caused typical Bakanae symptoms such as etiolation and
abnormal succulent rice growth. To reveal the relationship
between mycelial growth in the infected tissues and Bakanae
disease progression, we have established a reliable quantification
method
using real time PCR that employs a primer
pair and dual-labeled probe specific to a unigene encoding
F. fujikuroi PNG1 (FfPNG1), which is located upstream of
the fumonisin biosynthesis gene cluster. Plotting the crossing
point (CP) values from the infected tissue DNAs on a standard
curve revealed the active fungal growth of FfB14 in
the root and crown of rice seedlings, while the growth rate
of FfB20 in rice was more than 4 times lower than FfB14.
Massive infective mycelial growth of FfB14 was evident in
rice stems and crown; however, FfB20 did not exhibit vigorous
growth. Our quantitative evaluation system is applicable
for the identification of fungal virulence factors other
than gibberellin.
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Citations
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Istiaq Ahmed, Gareth J. Norton, Stephen Woodward
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Elena A. Tsavkelova
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Yeon-Jae Hur, Sais Beul Lee, Tae Heon Kim, Tackmin Kwon, Jong-Hee Lee, Dong-Jin Shin, Soo-Kwon Park, Un-Ha Hwang, Jun Hyeon Cho, Young-Nam Yoon, Un-Sang Yeo, You-Chun Song, Do-Yeon Kwak, Min-Hee Nam, Dong-Soo Park
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- DBA/2 Mouse as an Animal Model for Anti-influenza Drug Efficacy Evaluation
-
Jin Il Kim , Sehee Park , Sangmoo Lee , Ilseob Lee , Jun Heo , Min-Woong Hwang , Joon-Yong Bae , Donghwan Kim , Seok-Il Jang , Mee Sook Park , Man-Seong Park
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J. Microbiol. 2013;51(6):866-871. Published online December 19, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3428-7
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330
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15
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Abstract
PDF
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Influenza viruses are seasonally recurring human pathogens.
Vaccines and antiviral drugs are available for influenza.
However, the viruses, which often change themselves via
antigenic drift and shift, demand constant efforts to update
vaccine antigens every year and develop new agents with
broad-spectrum antiviral efficacy. An animal model is critical
for such efforts. While most human influenza viruses are
unable to kill BALB/c mice, some strains have been shown
to kill DBA/2 mice without prior adaptation. Therefore, in
this study, we explored the feasibility of employing DBA/2
mice as a model in the development of anti-influenza drugs.
Unlike the BALB/c strain, DBA/2 mice were highly susceptible
and could be killed with a relatively low titer (50%
DBA/2 lethal dose = 102.83 plaque-forming units) of the A/
Korea/01/2009 virus (2009 pandemic H1N1 virus). When
treated with a neuraminidase inhibitor, oseltamivir phosphate,
infected DBA/2 mice survived until 14 days postinfection.
The reduced morbidity of the infected DBA/2
mice was also consistent with the oseltamivir treatment.
Taking these data into consideration, we propose that the
DBA/2 mouse is an excellent animal model to evaluate antiviral
efficacy against influenza infection and can be further
utilized for combination therapies or bioactivity models of
existing and newly developed anti-influenza drugs.
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- NOTE] Lysinibacillus jejuensis sp. nov., Isolated from Swinery Waste
-
Ji-Young Kim , So-Hyun Park , Duck-Chul Oh , Young-Ju Kim
-
J. Microbiol. 2013;51(6):872-876. Published online December 19, 2013
-
DOI: https://doi.org/10.1007/s12275-013-2500-7
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278
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8
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Abstract
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A Gram-positive, endospore-forming, rod-shaped bacterium, designated strain N2-5T, was isolated from swinery waste collected in Jeju, Republic of Korea. A phylogenetic analysis based on 16S rRNA gene sequences demonstrated
that strain N2-5T formed a phyletic group within the phylum
Firmicutes with less than 97.0% similarities to members of
the genus Lysinibacillus, its nearest phylogenetic neighbors.
The highest levels of sequence similarity to the isolate were
observed against Lysinibacillus xylanilyticus XDB9T (96.8%),
Lysinibacillus macroides LMG 18474T (95.6%), and Lysinibacillus
parviboronicapiens BAM-582 T (95.6%). The organism
grew optimally at 30°C and pH 7 and in the presence of 1–3%
(w/v) NaCl. Strain N2-5T was chemotaxonomically characterized
by possessing menaquinone-7 (MK-7) as the major
menaquinone, and iso-C15:0 (54.9%), iso-C17:1ω10c (12.0%),
and C16:1ω7c alcohol (11.8%) as the predominant fatty acids.
The genomic DNA G+C content of the novel strain was
43.3 mol% and the cell-wall peptidoglycan was type A4α.
The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol,
and phosphatidylethanolamine. Based on
its phenotypic properties and phylogenetic data, strain N2-5T
(=DSM 28310T =KCTC13837 T) represents a novel species in
the genus Lysinibacillus, for which the name Lysinibacillus
jejuensis sp. nov. is proposed.
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Journal of Microbiology.2019; 57(7): 562. CrossRef -
Lysinibacillus alkalisoli sp. nov., isolated from saline–alkaline soil
Ji-Quan Sun, Lian Xu, Xiao-Lei Wu
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Lysinibacillus acetophenoni sp. nov., a solvent-tolerant bacterium isolated from acetophenone
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International Journal of Systematic and Evolutionary Microbiology
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Antonie van Leeuwenhoek.2015; 107(1): 157. CrossRef
- NOTE] Next-Generation Sequencing-Based Transcriptome Analysis of L-Lysine-Producing Corynebacterium glutamicum ATCC 21300 Strain
-
Hong-Il Kim , Jae-Young Nam , Jae-Yong Cho , Chang-Soo Lee , Young-Jin Park
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J. Microbiol. 2013;51(6):877-880. Published online December 19, 2013
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DOI: https://doi.org/10.1007/s12275-013-3236-0
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270
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Abstract
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In the present study, 151 genes showed a significant change
in their expression levels in Corynebacterium glutamicum
ATCC 21300 compared with those of C. glutamicum ATCC
13032. Of these 151 genes, 56 genes (2%) were up-regulated
and 95 genes (3%) were down-regulated. RNA sequencing
analysis also revealed that 11 genes, involved in the L-lysine
biosynthetic pathway of C. glutamicum, were up- or downregulated
compared with those of C. glutamicum ATCC
13032. Of the 151 genes, 10 genes were identified to have
mutations including SNP (9 genes) and InDel (1 gene). This
information will be useful for genome breeding of C. glutamicum
to develop an industrial amino acid-producing strain
with minimal mutation.
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Journal of Industrial Microbiology and Biotechnology.2017; 44(2): 181. CrossRef - Transcriptome and Gene Ontology (GO) Enrichment Analysis Reveals Genes Involved in Biotin Metabolism That Affect l-Lysine Production in Corynebacterium glutamicum
Hong-Il Kim, Jong-Hyeon Kim, Young-Jin Park
International Journal of Molecular Sciences.2016; 17(3): 353. CrossRef
- NOTE] Isolation and Characterization of Histamine-Producing Bacteria from Fermented Fish Products
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Jin Seok Moon , So-Young Kim , Kyung-Ju Cho , Seung-Joon Yang , Gun-Mook Yoon , Hyun-Ju Eom , Nam Soo Han
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J. Microbiol. 2013;51(6):881-885. Published online December 19, 2013
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DOI: https://doi.org/10.1007/s12275-013-3333-0
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563
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10
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Abstract
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Histamine is mainly produced by microorganisms that are
found in fermented foods, and is frequently involved in food
poisoning. Two histamine-producing bacteria were isolated
from fermented fish products, anchovy sauce, and sand lance
sauce by using a histidine decarboxylating medium. The
species were identified as Bacillus licheniformis A7 and B.
coagulans SL5. Multiplex PCR analysis showed the presence
of the conserved histidine decarboxylase (hdc) gene in the
chromosome of these bacteria. B. licheniformis A7 and B.
coagulans SL5 produced the maximum amount of histamine
(22.3±3.5 and 15.1±1.5 mg/L, respectively). As such, they
were determined to be potential histamine-producing bacteria
among the tested cultures.
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The Journal of General and Applied Microbiology.2022; 68(5): 213. CrossRef - Isolation and Identification of Aroma-producing Yeast from Mackerel Fermentation Broth and Its Fermentation Characteristics
Yu Wu, Xiao’e Chen, Xubo Fang, Lili Ji, Fang Tian, Hui Yu, Yan Chen
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Jianan Sun, Xiaohang Yu, Bohuan Fang, Lei Ma, Changhu Xue, Zhaohui Zhang, Xiangzhao Mao
International Journal of Food Science & Technology.2016; 51(1): 133. CrossRef - Characterization of Tryptamine-Producing Bacteria Isolated from Commercial Salted and Fermented Sand Lance Ammodytes personatus Sauces
In-Seon Um, Tae-Ok Kim, Hee-Dai Kim, Kwon-Sam Park
Korean Journal of Fisheries and Aquatic Sciences.2016; 49(6): 792. CrossRef - Relationship between chemical characteristics and bacterial community of a Korean salted-fermented anchovy sauce, Myeolchi-Aekjeot
Hae-Won Lee, Yun-Jeong Choi, In Min Hwang, Sung Wook Hong, Mi-Ai Lee
LWT.2016; 73: 251. CrossRef - Isolation and Characterization of Putrescine-producing Bacteria in Commercially Available Sauces Made from Salted and Fermented Sand Lance Ammodytes personatus
In-Seon Um, Tae-Ok Kim, Kwon-Sam Park
Korean Journal of Fisheries and Aquatic Sciences.2016; 49(5): 573. CrossRef
- Author’s Correction] Experimental Phasing Using Zinc and Sulfur Anomalous Signals Measured at the Zinc Absorption Peak
-
Sangmin Lee , Min-Kyu Kim , Chang-Jun Ji , Jin-Won Lee , Sun-Shin Cha
-
J. Microbiol. 2013;51(6):886-886.
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DOI: https://doi.org/10.1007/s12275-013-0725-5
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Abstract
PDF
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In the article by Lee et al. published in the Journal of Microbiology 2013; 51, 639-643. Acknowledgement should appear as
shown below.
This work was supported by the National Research Foundation of Korea Grant NRF-2012R1A2A2A02005978, the CAP
through Korea Research Council of Fundamental Science Technology (KRCF), Korea Institute of Science and Technology
(KIST), & Korea Institute of Ocean Science and Technology (KIOST), the Marine and Extreme Genome Research Center
program, and the Development of Biohydrogen Production Technology Using Hyperthermophilic Archaea program of MOF.
Works performed at Hanyang University were supported by Basic Science Research Program through the National Research
Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (MEST) (KRF-2008-313-C00774).
- Author’s Correction] Arenimonas aquaticum sp. nov., a Member of the Gammaproteobacterium, Isolated from a Freshwater Reservoir
-
A-Ram Kim , Siwon Lee , Kyudong Han , Tae-Young Ahn
-
J. Microbiol. 2013;51(6):887-887.
-
DOI: https://doi.org/10.1007/s12275-013-0728-x
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236
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1
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Abstract
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In the article by Kim et al. that appears in Journal of Microbiology 2012; 50, 354-358. The novel species name Arenimonas
aquaticum should be changed as Arenimonas aquatica because the genus name Arenimonas is of the feminine gender. The
adjective used as specific epithet must be in the feminine gender as well. In addition, type strain NA-09T =KACC14663T should
be changed as type strain NA-09T =KACC14662T.
-
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- Assessing the Ecotoxicity of Eight Widely Used Antibiotics on River Microbial Communities
María Rosa Pino-Otín, Guillermo Lorca, Elisa Langa, Francisco Roig, Eva M. Terrado, Diego Ballestero
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- Erratum] Bioactive Activities of Natural Products against Herpesvirus Infection
-
Myoungki Son , Minjung Lee , Gi-Ho Sung , Taeho Lee , Yu Su Shin , Hyosun Cho , Paul M. Lieberman , Hyojeung Kang
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J. Microbiol. 2013;51(6):888-888.
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DOI: https://doi.org/10.1007/s12275-013-0726-z
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Abstract
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In the article by Son et al. published in the Journal of Microbiology 2013; 51, 545-551.
- Acknowledgement should appear as shown below.
We gratefully acknowledge financial supports of Next Generation BioGreen 21 Program (Project No. PJ009508) and
Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ009241) from
Rural Development Administration, Republic of Korea.
- Co-corresponding author’s (Dr. Paul M. Lieberman) affiliation should appear as shown below.
6The Wistar Institute, Philadelphia, PA 19104, USA
- Erratum] Genetic Organization and Conjugal Plasmid DNA Transfer of pHP69, a Plasmid from a Korean Isolate of Helicobacter pylori
-
Jung-Soo Joo , Jae-Young Song , Seung-Chul Baik , Woo-Kon Lee , Myung-Je Cho , Kon-Ho Lee , Hee-Shang Youn , Ji-Hyun Seo , Kwang-Ho Rhee , Hyung-Lyun Kang
-
J. Microbiol. 2013;51(6):889-889.
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DOI: https://doi.org/10.1007/s12275-013-0727-y
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Abstract
PDF
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In the article by Joo et al. published in the Journal of Microbiology 2012; 50, 955-961. Dr. YoungAh Cho, one of coauthor’s
name was inadvertently missing. Complete authors information is as follow.
Jung-Soo Joo4, Jae-Young Song1,3, Seung-Chul Baik1,3, Woo-Kon Lee1,3, Myung-Je Cho1,3, Kon-Ho Lee1,3,
YoungAh Cho1, Hee-Shang Youn2, Ji-Hyun Seo2, Kwang-Ho Rhee1,3, and Hyung-Lyun Kang1,3*