- Volume 46(6); December 2008
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Journal Articles
- Molecular Identification of Fecal Pollution Sources in Water Supplies by Host-Specific Fecal DNA Markers and Terminal Restriction Fragment Length Polymorphism Profiles of 16S rRNA Gene
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Ju-Yong Jeong , Kyung-Ik Gil , Kyong-Hee Lee , Jong-Ok Ka
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J. Microbiol. 2008;46(6):599-607. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0174-3
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Specific fecal DNA markers were investigated for major pollution sources, cow, human, and pig, and occurrence of the identified markers was analyzed in river waters using Terminal Restriction Fragment Length Polymorphism (T-RFLP) techniques and sequencing of 16S rDNA of Bacteroides-Prevotella. The unique and specific DNA markers for cow and human were identified as a 222 bp and 60 bp peak in HaeIII T-RFLP profiles, respectively, and the pig-specific marker was not identified but the unique T-RFLP profile of pig could be used as a substitution. Human-specific marker was detected in most of the river waters tested (92.1%) and T-RFLP profiles of river waters were shown to be similar to those of human feces. Cluster analysis of T-RFLP data showed that the fecal sources were multiple (human plus cow and human plus dairy cow) in most of the river waters. The phylogenetic analysis for the clones recovered from the fecal and water samples showed that the clones from cow formed a discreet cluster from those of other sources. The other clones from human, pig, and river water formed two groups all together. The results of this study could be used to identify and control the fecal pollution source in the bodies of water in Korea.
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- Microbial community analysis and identification of alternative host-specific fecal indicators in fecal and river water samples using pyrosequencing
Ju-Yong Jeong, Hee-Deung Park, Kyong-Hee Lee, Hang-Yeon Weon, Jong-Ok Ka
The Journal of Microbiology.2011; 49(4): 585. CrossRef - Application of an Integrated Community Analysis Approach for Microbial Source Tracking in a Coastal Creek
Yiping Cao, Laurie C. Van De Werfhorst, Bram Sercu, Jill L. S. Murray, Patricia A. Holden
Environmental Science & Technology.2011; 45(17): 7195. CrossRef
- Targeting the rpoB Gene Using Nested PCR-Restriction Fragment Length Polymorphism for Identification of Nontuberculous Mycobacteria in Hospital Tap Water
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Ji-Hyun Shin , Hae-Kyung Lee , Eun-Jin Cho , Jae-Yon Yu , Yeon-Ho Kang
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J. Microbiol. 2008;46(6):608-614. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0102-6
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262
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Nontuberculous mycobacteria (NTM) are ubiquitous in the environment and can cause nosocomial infections in immunocompromised patients. Recently the presence of NTM in public drinking water and hospital water distribution systems has been reported. Their ability to form biofilms and their resistance to chlorine both contribute to their survival and colonization in water distribution systems. Here we analyzed thirty-two hospital tap water samples that were collected from different locations in three hospitals so as to evaluate the prevalence of NTM species. The water samples were concentrated by membrane filtration and then eluted with sterilized water following sonication. Two-step direct PCR targeting the rpoB gene, restriction fragment length polymorphism (RFLP) using the MspI restriction enzyme, and sequence analysis were performed for identification of NTM to the species level. The sequences of each PCR product were analyzed using BLASTN. Seven samples (7/32, 21.9%) were positive for NTM as determined by nested-PCR. The PCR-RFLP results indicated five different patterns among the seven positive PCR samples. The waterborn NTM were identified, including M. peregrinum, M. chelonae (2 cases), M. abscessus, M. gordonae (2 cases), and Mycobacterium sp. JLS. The direct two-step PCR-RFLP method targeting the rpoB gene was effective for the detection and the differentiation of NTM species from hospital tap water.
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Citations
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- Occurrence of opportunistic pathogens in private wells after major flooding events: A four state molecular survey
Kris Mapili, William J. Rhoads, Mary Coughter, Kelsey J. Pieper, Marc A. Edwards, Amy Pruden
Science of The Total Environment.2022; 826: 153901. CrossRef - Omadacycline for management of Mycobacterium abscessus infections: a review of its effectiveness, place in therapy, and considerations for use
Ashley R. Rizzo, Nader H. Moniri
BMC Infectious Diseases.2022;[Epub] CrossRef - Threat in Water for Drinking and Domestic Use
Dursun Atik, Sukrü Oksuz, Elif Ozturk, Emel Çaliskan, Nida Akar, Mehmet Ali Sungur
The International Journal of Mycobacteriology.2021; 10(2): 188. CrossRef - Prevalence of Non-Tuberculous Mycobacteria in Hospital Waters of Major Cities of Khuzestan Province, Iran
Azar Dokht Khosravi, Abdolrazagh Hashemi Shahraki, Mohammad Hashemzadeh, Rasa Sheini Mehrabzadeh, Ali Teimoori
Frontiers in Cellular and Infection Microbiology.2016;[Epub] CrossRef - Nosocomial pseudo-outbreak of Mycobacterium gordonae associated with a hospital's water supply contamination: a case series of 135 patients
Marija Zlojtro, Mateja Jankovic, Miroslav Samarzija, Ljiljana Zmak, Vera Katalinic Jankovic, Mihaela Obrovac, Igor Zlojtro, Marko Jakopovic
Journal of Water and Health.2015; 13(1): 125. CrossRef - Endosymbiotic Mycobacterium chelonae in a Vermamoeba vermiformis strain isolated from the nasal mucosa of an HIV patient in Lima, Peru
Alfonso Martín Cabello-Vílchez, Rosmery Mena, Johanna Zuñiga, Pablo Cermeño, Carmen Ma Martín-Navarro, Ana C. González, Atteneri López-Arencibia, María Reyes-Batlle, José E. Piñero, Basilio Valladares, Jacob Lorenzo-Morales
Experimental Parasitology.2014; 145: S127. CrossRef - Sacroiliitis secondary to catheter-related bacteremia due to Mycobacterium abscessus (sensu stricto)
Chrislène Laurens, Geneviève Héry-Arnaud, Raphael Chiron, Eric Oziol, Hélène Jean-Pierre, Nicolas Bouzinbi, Philippe Vande Perre, Anne-Laure Bañuls, Sylvain Godreuil
Annals of Clinical Microbiology and Antimicrobials.2014;[Epub] CrossRef - Mycobacterium abscessus Induces a Limited Pattern of Neutrophil Activation That Promotes Pathogen Survival
Kenneth C. Malcolm, E. Michelle Nichols, Silvia M. Caceres, Jennifer E. Kret, Stacey L. Martiniano, Scott D. Sagel, Edward D. Chan, Lindsay Caverly, George M. Solomon, Paul Reynolds, Donna L. Bratton, Jennifer L. Taylor-Cousar, David P. Nichols, Milene T.
PLoS ONE.2013; 8(2): e57402. CrossRef - Standardization of multilocus sequence typing scheme for Mycobacterium abscessus and Mycobacterium massiliense
Song Yee Kim, Young Ae Kang, Il Kwon Bae, Jae-Joon Yim, Moo Suk Park, Young Sam Kim, Se Kyu Kim, Joon Chang, Seok Hoon Jeong
Diagnostic Microbiology and Infectious Disease.2013; 77(2): 143. CrossRef - Mycobacterium abscessus multispacer sequence typing
Mohamed Sassi, Imen Ben Kahla, Michel Drancourt
BMC Microbiology.2013;[Epub] CrossRef - Diversity, Community Composition, and Dynamics of Nonpigmented and Late-Pigmenting Rapidly Growing Mycobacteria in an Urban Tap Water Production and Distribution System
S. Dubrou, J. Konjek, E. Macheras, B. Welté, L. Guidicelli, E. Chignon, M. Joyeux, J. L. Gaillard, B. Heym, T. Tully, G. Sapriel
Applied and Environmental Microbiology.2013; 79(18): 5498. CrossRef - Isolation of nontuberculous mycobacteria from hospital waters in Turkey
Gonca Erkose Genc, Elvira Richter, Zayre Erturan
APMIS.2013; 121(12): 1192. CrossRef - Mycobacteria in water, soil, plants and air: a review
K. Hruska, M. Kaevska
Veterinární medicína.2012; 57(12): 623. CrossRef - Molecular Survey of the Occurrence of Legionella spp., Mycobacterium spp., Pseudomonas aeruginosa, and Amoeba Hosts in Two Chloraminated Drinking Water Distribution Systems
Hong Wang, Marc Edwards, Joseph O. Falkinham, Amy Pruden
Applied and Environmental Microbiology.2012; 78(17): 6285. CrossRef -
Multilocus Sequence Analysis and
rpoB
Sequencing of Mycobacterium abscessus (Sensu Lato) Strains
Edouard Macheras, Anne-Laure Roux, Sylvaine Bastian, Sylvia Cardoso Leão, Moises Palaci, Valérie Sivadon-Tardy, Cristina Gutierrez, Elvira Richter, Sabine Rüsch-Gerdes, Gaby Pfyffer, Thomas Bodmer, Emmanuelle Cambau, Jean-Louis Gaillard, Beate Heym
Journal of Clinical Microbiology.2011; 49(2): 491. CrossRef
- Monitoring Nutrient Impact on Bacterial Community Composition during Bioremediation of Anoxic PAH-Contaminated Sediment
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Myungsu Kim , Seung Seob Bae , Mijin Seol , Jung-Hyun Lee , Young-Sook Oh
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J. Microbiol. 2008;46(6):615-623. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0097-z
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335
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Marine harbor sediments are frequently polluted with significant amount of polycyclic aromatic hydrocarbons (PAHs) some of which are naturally toxic, recalcitrant, mutagenic, and carcinogenic. To stimulate biodegradation of PAHs in PAH-contaminated sediments collected from near Gwangyang Bay, Korea, lactate was chosen as a supplementary carbonaceous substrate. Sediment packed into 600 ml air-tight jar was either under no treatment condition or lactate amended condition (1%, w/v). Microbial community composition was monitored by bacteria-specific and archaea-specific PCR-terminal restriction fragment length polymorphism (T-RFLP), in addition to measuring the residual PAH concentration. Results showed that lactate amendment enhanced biodegradation rate of PAHs in the sediment by 4 to 8 times, and caused a significant shift in archaebacterial community in terms of structure and diversity with time. Phylogenetic analysis of 23 archaeal clones with distinctive RFLP patterns among 288 archaeal clones indicated that majority of the archaeal members were closest to unculturable environmental rDNA clones from hydrocarbon-contaminated and/or methanogenesis-bearing sediments. Lactate amendment led to the enrichment of some clones that were most closely related to PAH-degrading Methanosarcina species. These results suggest a possible contribution of methanogenic community to PAH degradation and give us more insights on how to effectively remediate PAH-contaminated sediments.
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Qingguo Chen, Zhenzhen Li, Yu Chen, Mei Liu, Qiao Yang, Baikang Zhu, Jun Mu, Lijuan Feng, Zhi Chen
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Microbial Ecology.2011; 61(4): 871. CrossRef - Revealing archaeal diversity patterns and methane fluxes in Admiralty Bay, King George Island, and their association to Brazilian Antarctic Station activities
C.R. Nakayama, E. Kuhn, A.C.V. Araújo, P.C. Alvalá, W.J. Ferreira, R.F. Vazoller, V.H. Pellizari
Deep Sea Research Part II: Topical Studies in Oceanography.2011; 58(1-2): 128. CrossRef - Bioremediation of marine sediments contaminated by hydrocarbons: Experimental analysis and kinetic modeling
Francesca Beolchini, Laura Rocchetti, Francesco Regoli, Antonio Dell’Anno
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Research Support, Non-U.S. Gov'ts
- Dark Septate Endophyte (DSE) Fungi Isolated from Metal Polluted Soils: Their Taxonomic Position, Tolerance, and Accumulation of Heavy Metals In Vitro
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Yujie Zhang , Yan Zhang , Maojun Liu , Xiaodong Shi , Zhiwei Zhao
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J. Microbiol. 2008;46(6):624-632. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0163-6
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247
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To understand the possible role of the plant root associated fungi on metal tolerance, their role in the uptake of heavy metals and the potential transfer of these metal ions to the plant, three strains of dark septate endophytic (DSE) fungi were isolated from a waste smelter site in southwest China, and one strain was isolated from a non-contaminated site. According to molecular phylogenetic analysis of the ITS 1-5.8S rDNA-ITS 2 gene regions and morphological characteristics, one is identified as Exophiala pisciphila, and the other three are non-sporulating fungi under the experiment condition with the nearest phylogenetic affinities to the Thysanorea papuana strain EU041814. Tolerance and accumulation abilities of the three DSE strains for metals were investigated in liquid culture. Minimum inhibitory concentrations (MIC) of Pb, Zn, and Cd were determined. It was demonstrated that the tolerance of the DSE strains varied between metal species and strains. The E. pisciphila strain is able to accumulate lead and cadmium over 20% and 5% of dry weight of biomass, respectively. Partial of the sequestrated metals can be washed with CaCl2. Morphological and enzyme activity changes taking place in the presence of excessive Pb, Cd, and/or Zn also indicate that the mechanism of heavy metal tolerance and accumulation of the DSE strains would be a complex process. The findings indicated promising tolerance and accumulation of the DSE strains with potential values in metal cycling and restoration of soil and water system.
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to Metal Stress: Functional Importance and Relation to Metal Tolerance
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B. Shankar Naik
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Gordana Gajić, Lola Djurdjević, Olga Kostić, SneŽana Jarić, Miroslava Mitrović, Pavle Pavlović
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Theodora L. Nikolakopoulou , Eleni P. Giannoutsou , Adamandia A. Karabatsou , Amalia D. Karagouni
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J. Microbiol. 2008;46(6):633-640. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0080-8
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The presence of selected tetracycline resistance (TcR) genes was studied in different Greek seawater habitats, originated from wastewater treatment facilities, fishfarm, and coastal environments. The methods employed included assessment of the presence of twelve gene clusters by PCR, followed by hybridization with specific probes, in habitat extracted DNA, TcR bacteria, and exogenous isolated plasmids conferring TcR. The direct DNA-based analysis showed that tet(Α) and tet(K) genes were detected in all habitats, whilst tet(C) and tet(E) were present in fishfarm and wastewater effluent samples and tet(M) was detected in fishfarm and coastal samples. Resistance genes tet(A), tet(C), tet(K), and tet(M) were detected in 60 of the 89 isolates screened. These isolates were identified by fatty acid methyl ester analysis (FAME) as Stenotrophomonas, Acinetobacter, Pseudomonas, Bacillus, and Staphylococcus strains. The presence of the TcR genes in 15% of the bacterial isolates coincided with the presence of IncP plasmids. A habitat-specific dissemination of IncP alpha plasmids in wastewater effluent isolates and of IncP beta plasmids in fishfarm isolates was observed. Exogenous isolation demonstrated the presence of plasmids harbouring TcR genes in all the habitats tested. Plasmids were shown to carry tet(A), tet(C), tet(E), and tet(K) genes. It is concluded that TcR genes are widespread in the seawater habitats studied and often occur on broad host range plasmids that seem to be well disseminated in the bacterial communities.
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DOI: https://doi.org/10.1007/s12275-008-0159-2
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191
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A greenhouse test was carried out to examine the effects on tomato growth of application of purple nonsulfur bacterium Rhodopseudomonas sp. which had enhanced germination and growth of tomato seed under axenic conditions. The shoot length of tomato plant inoculated by Rhodopseudomonas sp. KL9 increased by 34.6% compared to that of control in 8 weeks of cultivation. During the same period, this strain increased 120.6 and 78.6% of dry weight of shoot and root of tomato plants, respectively. The formation ratio of tomato fruit from flower was also raised by inoculation of KL9. In addition, Rhodopseudomonas sp. KL9 treatment enhanced the fresh weight and lycopene content in the harvested tomato fruits by 98.3 and 48.3%, respectively compared to those of the uninoculated control. When the effect on the indigenous bacterial community and fate of the inoculated Rhodopseudomonas sp. KL9 were monitored by denaturing gradient gel electrophoresis analysis, its application did not affect the native bacterial community in tomato rhizosphere soil, but should be repeated to maintain its population size. This bacterial capability may be applied as an environment-friendly biofertilizer to cultivation of high quality tomato and other crops including lycopene-containing vegetables and fruits.
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Tomorn Nunkaew, Duangporn Kantachote, Teruhiko Nitoda, Hiroshi Kanzaki, Raymond J. Ritchie
Carbohydrate Polymers.2015; 115: 334. CrossRef - Endophytic bacteria in plant tissue culture: differences between easy- and difficult-to-propagate Prunus avium genotypes
M. Quambusch, A. M. Pirttila, M. V. Tejesvi, T. Winkelmann, M. Bartsch
Tree Physiology.2014; 34(5): 524. CrossRef - Characteristics of purple nonsulfur bacteria grown under Stevia
residue extractions
J. Xu, Y. Feng, Y. Wang, X. Lin
Letters in Applied Microbiology.2013; 57(5): 420. CrossRef - Purple Phototrophic Bacterium Enhances Stevioside Yield by Stevia rebaudiana Bertoni via Foliar Spray and Rhizosphere Irrigation
Jing Wu, Yiming Wang, Xiangui Lin, Gustavo Bonaventure
PLoS ONE.2013; 8(6): e67644. CrossRef - Trichoderma-Enriched Biofertilizer Enhances Production and Nutritional Quality of Tomato (Lycopersicon esculentum Mill.) and Minimizes NPK Fertilizer Use
Abul Hossain Molla, Md. Manjurul Haque, Md. Amdadul Haque, G. N. M. Ilias
Agricultural Research.2012; 1(3): 265. CrossRef - Exopolysaccharide-Producing Plant Growth-Promoting Rhizobacteria Under Salinity Condition
S.K. UPADHYAY, J.S. SINGH, D.P. SINGH
Pedosphere.2011; 21(2): 214. CrossRef - Elevated Ground-Level O3 Changes the Diversity of Anoxygenic Purple Phototrophic Bacteria in Paddy Field
Youzhi Feng, Xiangui Lin, Yongchang Yu, Jianguo Zhu
Microbial Ecology.2011; 62(4): 789. CrossRef - Field Evidence for the Potential of Rhodobacter capsulatus as Biofertilizer for Flooded Rice
Hosny Gamal-Eldin, Khaled Elbanna
Current Microbiology.2011; 62(2): 391. CrossRef
- Diversity of Bacterial Community in Freshwater of Woopo Wetland
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Keun Sik Baik , Seong Chan Park , Eun Mi Kim , Kyung Sook Bae , Jae-Hyung Ahn , Jong-Ok Ka , Jongsik Chun , Chi Nam Seong
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J. Microbiol. 2008;46(6):647-655. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0135-x
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182
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Diversity of bacterial community in water layer of Woopo wetland was investigated. Cultivable bacterial strains were isolated by the standard dilution plating technique and culture-independent 16S rRNA gene clones were obtained directly from DNA extracts of a water sample. Amplified rDNA restriction analysis (ARDRA) was applied onto both of the isolates and 16S rRNA gene clones. Rarefaction curves, coverage rate and diversity indices of ARDRA patterns were calculated. Representative isolates and clones of all the single isolate/clone phylotype were partially sequenced and analyzed phylogenetically. Sixty-four and 125 phylotypes were obtained from 203 bacterial isolates and 235 culture-independent 16S rRNA gene clones, respectively. Bacterial isolates were composed of 4 phyla, of which Firmicutes (49.8%) and Actinobacteria (32.0%) were predominant. Isolates were affiliated with 58 species. Culture-independent 16S rRNA gene clones were composed of 8 phyla, of which Proteobacteria (62.2%), Actinobacteria (15.5%), and Bacteroidetes (13.7%) were predominant. Diversity of 16S rRNA gene clones originated from cultivation-independent DNA extracts was higher than that of isolated bacteria.
Journal Article
- Biologically Active and C-Amidated HinnavinII-38-Asn Produced from a Trx Fusion Construct in Escherichia coli
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Chang Soo Kang , Seung-Yeol Son , In Seok Bang
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J. Microbiol. 2008;46(6):656-661. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0214-z
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189
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9
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The cabbage butterfly (Artogeia rapae) antimicrobial peptide hinnavinII as a member of cecropin family is synthesized as 37 residues in size with an amidated lysine at C-terminus and shows the humoral immune response to a bacterial invasion. In this work, a synthetic gene for hinnavinII-38-Asn (HIN) with an additional amino acid asparagine residue containing amide group at C-terminus was cloned into pET-32a(+) vector to allow expression of HIN as a Trx fusion protein in Escherichia coli strain BL21 (DE3) pLysS. The resulting expression level of the fusion protein Trx-HIN could reach 15~20% of the total cell proteins and more than 70% of the target proteins were in soluble form. The fusion protein could be purified successfully by HiTrap Chelating HP column and a high yield of 15 mg purified fusion protein was obtained from 80 ml E. coli culture. Recombinant HIN was readily obtained by enterokinase cleavage of the fusion protein followed by FPLC chromatography, and 3.18 mg pure active recombinant HIN was obtained from 80 ml culture. The molecular mass of recombinant HIN determined by MALDI-TOF mass spectrometer is 4252.084 Da which matches the theoretical mass (4252.0 Da) of HIN. Comparing the antimicrobial activities of the recombinant hinnavinII with C-amidated terminus to that without an amidated C-terminus, we found that the amide of asparagine at C-terminus of hinnavinII improved its potency on certain microorganism such as E. coli, Enterobacter cloacae, Bacillus megaterium, and Staphylococcus aureus.
Research Support, Non-U.S. Gov'ts
- Stable Expression and Secretion of Polyhydroxybutyrate Depolymerase of Paucimonas lemoignei in Escherichia coli
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Se Whan Park , Moon Gyu Chung , Hwa Young Lee , Jeong Yoon Kim , Young Ha Rhee
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J. Microbiol. 2008;46(6):662-669. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0283-z
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213
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1
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An efficient strategy for the expression and secretion of extracellular polyhydroxybutyrate depolymerase (PhaZ1) of Paucimonas lemoignei in Escherichia coli was developed by employing the signal peptide of PhaZ1 and a truncated ice nucleation protein anchoring motif (INPNC). Directly synthesized mature form of PhaZ1 was present in the cytoplasm of host cells as inclusion bodies, while a construct containing PhaZ1 and its own N-terminal signal peptide (PrePhaZ1) enabled the secretion of active PhaZ1 into the extracellular medium. However, the PrePhaZ1 construct was harmful to the host cell and resulted in atypical growth and instability of the plasmid during the cultivation. In contrast, INPNC-PhaZ1 and INPNCPrePhaZ1 fusion constructs did not affect growth of host cells. INPNC-PhaZ1 was successfully displayed on the cell surface with its fusion form, but did not retain PhaZ1 activity. In the case of INPNC-PrePhaZ1, the initially synthesized fusion form was separated by precise cleavage of the signal peptide, and active PhaZ1 was consequently released into the culture medium. The amount of PhaZ1 derived from E. coli (INPNC-PrePhaZ1) was almost twice as great as that directly expressed from E. coli (PrePhaZ1), and was predominantly (approximately 85%) located in the periplasm when cultivated at 22°C but was efficiently secreted into the extracellular medium when cultivated at 37°C.
- Cys-92, Cys-95, and the C-Terminal 12 Residues of the Vibrio harveyi Ferric Uptake Regulator (Fur) are Functionally Inessential
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Kun Sun , Shuang Cheng , Min Zhang , Fang Wang , Li Sun
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J. Microbiol. 2008;46(6):670-680. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0113-3
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261
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10
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Ferric uptake regulator (Fur) is a global regulator involved in multiple aspects of bacterial life. The gene encoding the Vibrio harveyi Fur (FurVh) was cloned from a pathogenic V. harveyi strain isolated from diseased fish. FurVh shares 77% overall sequence identity with the Escherichia coli Fur (FurEc) and could complement a mutant of FurEc. Like FurEc, FurVh possesses two cysteine residues at positions 92 and 95, yet unlike FurEc, in which these cysteine residues constitute part of the metal ion coordination site and hence are vital to the repressor activity, C92 and C95 of FurVh proved to be functionally inessential. Further study identified a Vibrio Fur signature sequence, which is preserved in all the ten Vibrio Fur proteins that have been discovered to date but in none of the non-vibrio Fur proteins. Site-directed and random mutation analyses of the signature residues, the cysteine residues, and seven highly charged amino acid residues indicated that D9, H32, C137, and K138 of FurVh are functionally important but D9, C137, and K138 can be replaced by more than one functional substitutes. Systematic deletion analysis demonstrated that the C-terminal 12 residues of FurVh are functionally inessential. These results (i) indicated that the activation mechanism, or certain aspects of which, of FurVh is possibly different from that of FurEc; and (ii) suggested that it is not very likely that the C-terminal 12 residues play any significant role in the activation or stability of FurVh; and (iii) provided insights into the potential function of the local structure involving C137 and K138.
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Citations
Citations to this article as recorded by

- Comparative analysis of the survival and gene expression of pathogenic strainsVibrio harveyiafter starvation
Jingjing Sun, Xiaojian Gao, Jiang Qun, Xuedi Du, Keran Bi, Xiaojun Zhang, Li Lin, Alejandra Bravo
FEMS Microbiology Letters.2016; 363(22): fnw250. CrossRef - The FUR (ferric uptake regulator) superfamily: Diversity and versatility of key transcriptional regulators
María F. Fillat
Archives of Biochemistry and Biophysics.2014; 546: 41. CrossRef - DNA adenine methylase is involved in the pathogenesis of Edwardsiella tarda
Kun Sun, Xu-dong Jiao, Min Zhang, Li Sun
Veterinary Microbiology.2010; 141(1-2): 149. CrossRef - Experimental and computational characterization of the ferric uptake regulator from Aliivibrio salmonicida (Vibrio salmonicida)
Hege Lynum Pedersen, Rafi Ahmad, Ellen Kristin Riise, Hanna-Kirsti Schrøder Leiros, Stefan Hauglid, Sigrun Espelid, Bjøn Olav Brandsdal, Ingar Leiros, Nils-Peder Willassen, Peik Haugen
The Journal of Microbiology.2010; 48(2): 174. CrossRef - Helicobacter pylori apo-Fur regulation appears unconserved across species
Shana Miles, Beth M. Carpenter, Hanan Gancz, D. Scott Merrell
The Journal of Microbiology.2010; 48(3): 378. CrossRef - Construction of an attenuated Pseudomonas fluorescens strain and evaluation of its potential as a cross-protective vaccine
Huan-ran Wang, Yong-hua Hu, Wei-wei Zhang, Li Sun
Vaccine.2009; 27(30): 4047. CrossRef - A ZnS4 Structural Zinc Site in the Helicobacter pylori Ferric Uptake Regulator
Sylvia Vitale, Caroline Fauquant, David Lascoux, Kristine Schauer, Christine Saint-Pierre, Isabelle Michaud-Soret
Biochemistry.2009; 48(24): 5582. CrossRef - Crystal structure of the Vibrio cholerae ferric uptake regulator (Fur) reveals insights into metal co‐ordination
Md. Arif Sheikh, Garry L. Taylor
Molecular Microbiology.2009; 72(5): 1208. CrossRef - Attenuation ofEdwardsiella tardaVirulence by Small Peptides That Interfere with LuxS/Autoinducer Type 2 Quorum Sensing
Min Zhang, Xu-dong Jiao, Yong-hua Hu, Li Sun
Applied and Environmental Microbiology.2009; 75(12): 3882. CrossRef - Domain analysis of the Edwardsiella tarda ferric uptake regulator
Kun Sun, Shuang Cheng, Fang Wang, Li Sun
The Journal of General and Applied Microbiology.2009; 55(5): 351. CrossRef
- Genus-Specific Distribution and Pathovar-Specific Variation of the Glycinecin R Gene Homologs in Xanthomonas genomes
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Eunjung Roh , Sunggi Heu , Eunpyo Moon
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J. Microbiol. 2008;46(6):681-686. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0209-9
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196
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Xanthomonas axonopodis pv. glycines produces bacteriocins called glycinecin, and two glycinecin genes, glyA and glyR, were reported previously. In this paper, we describe genomic distribution and variation of the glyR gene revealed by extensive Southern hybridization analysis. In contrast to the glyA gene present only in X. axonopodis pv. glycines, the glyR gene was found to be distributed widely in all the pathovars of Xanthomas genus. It was also found that the glyR gene is a multigene family while the glyA is a single copy gene. Moreover, the copy number and the variation of the glyR multigene are unique to each pathovar of Xanthomonas. The uniqueness can be easily detected by the patterns resulted from Southern hybridization using the genomic digests. Thus, we suggest the glyR gene can serve as a useful genus-specific and pathovarspecific DNA marker for Xanthomonas. One of the glyR homologs was further isolated from X. axonopodis pv. glycines, and analyzed to be functional with strong inhibitory activity against several members of Xanthomonas.
- Detection of Pseudomonas aeruginosa Carried a New Array of Gene Cassettes within Class 1 Integron Isolated from a Teaching Hospital in Nanjing, China
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Yuan Wu† , Hui Li† , Jun Li , Zu Hu Huang
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J. Microbiol. 2008;46(6):687-691. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0021-6
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262
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We report here novel array of gene cassettes found in single variable region of class 1 integron disseminated in Pseudomonas aeruginosa isolated from a teaching hospital in Nanjing, Jiangsu Province, China. 29 of 47 (61%) P. aeruginosa strains were confirmed haboured class 1 integron, and all the positive strains have the same variable region confirmed by PCR and RFLP methods. The variable region contained an unreported order of four gene cassettes aac(6’)-II-aadA13-cmlA8-oxa 10. Of those, cmlA8 gene was a variant of cmlA5 encoding non-enzymatic protein which putatively confer resistance to chloramphenicol. Susceptibility testing revealed multidrug-resistant mechanisms were involved in the class 1 integron positive clinical isolates. And the class 1 integron located on an about 15 kb transferable plasmid was certified by conjugation experiment and plasmid DNA analysis. The macro restriction profile indicated those clinical strains were clonally related.
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Journal Article
- Carbon Source Dependent Dynamics of the Ccr4-Not Complex in Saccharomyces cerevisiae
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Joakim Norbeck
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J. Microbiol. 2008;46(6):692-696. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0122-2
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256
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3
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We have investigated the composition of the conserved Ccr4-Not complex during different physiological states of Saccharomyces cerevisiae. Major changes were found, most notably in the expression of the central scaffold protein Not1p, which was strongly reduced in the absence of glucose. The low expression of Not1p was also evident from the inability of Pop2p to co-purify Not1p in cells from cultures lacking glucose. However, Not1p was still essential under conditions of low expression. The downregulation of Not1p indicates that many of the Ccr4-Not complex components are likely to have roles outside of the complex. We suggest that the use of different carbon sources will be a good starting point to unravel these functions.
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Citations
Citations to this article as recorded by

- HIPK family kinases bind and regulate the function of the CCR4-NOT complex
Alfonso Rodriguez-Gil, Olesja Ritter, Juliane Hornung, Hilda Stekman, Marcus Krüger, Thomas Braun, Elisabeth Kremmer, Michael Kracht, M. Lienhard Schmitz, Anne Spang
Molecular Biology of the Cell.2016; 27(12): 1969. CrossRef - Ccr4-Not complex: the control freak of eukaryotic cells
Jason E. Miller, Joseph C. Reese
Critical Reviews in Biochemistry and Molecular Biology.2012; 47(4): 315. CrossRef - The Ccr4–Not complex
Martine A. Collart, Olesya O. Panasenko
Gene.2012; 492(1): 42. CrossRef
Research Support, Non-U.S. Gov'ts
- Organization of Three rRNA (rrn) Operons from Sphingobium chungbukense DJ77
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Sun-Mi Yeon , Beom-Soon Choi , Young-Chang Kim
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J. Microbiol. 2008;46(6):697-703. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0193-0
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211
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4
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The nucleotide sequences of all three rRNA operons (rrnA, rrnB, and rrnC) of Sphingobium chungbukense DJ77 were determined. The three rrn operons have the same gene order (16S rRNA-tRNAIle-tRNAAla-23S rRNA-5S rRNA-tRNAfMet). The nucleotide sequences were identical over a 5,468 bp region spanning the 16S rRNA gene to the 5S rRNA gene. Variability was observed in the 5S rRNA-tRNAfMet spacer sequence of rrnB. The tRNAfMet gene sequences were identical except for two bases (T5794 and A5871 in rrnB, T5942 and A5956 in rrnA, but C5942 and G5956 in rrnC). Comparative sequence analyses of ribosomal RNA operons from DJ77 with those of the class Alphaproteobacteria, to which the genus Sphingobium belongs, reveal close evolutionary relationships with other members of the order Sphingomonadales.
- Molecular and Phylogenetic Characterization of Spodoptera litura Granulovirus
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Yong Wang , Jae Young Choi , Jong Yul Roh , Soo Dong Woo , Byung Rae Jin , Yeon Ho Je
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J. Microbiol. 2008;46(6):704-708. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0133-z
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248
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24
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Baculovirus, Spodoptera litura granulovirus (SlGV) was isolated from the infected S. litura larvae, and was characterized. The granule of SlGV was ovoidal shape with an approximate size of 240~340 nm×140~180 nm. Each granule contained one single rod-shape virion with a mean size of 180~200 nm×20~40 nm. Restriction endonuclease fragment analysis estimated that the total genome size of SlGV is about 115 kb. Necleotide sequence analysis of the granulin gene showed that the gene encodes 249 amino acids with a predicted molecular mass of 29 kDa. When the phylogenic relationship was analyzed using the nucleotide sequence of the granulin gene, SlGV was most closely related to Trichoplusia ni granulovirus (TnGV) and Xestia c-nigrum granulovirus (XcGV) which belong to Type I granulovirus.
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- The Individual and Combined Entomopathogenic Activity of a Spodoptera frugiperda Multiple Nucleopolyhedrovirus and a Type I Spodoptera frugiperda Granulovirus on S. frugiperda Larvae
Magali Ordóñez-García, Juan Carlos Bustillos-Rodríguez, José de Jesús Ornelas-Paz, Miguel Ángel Salas-Marina, Octavio Jhonathan Cambero-Campos, Carlos Horacio Acosta-Muñiz, David Ignacio Berlanga-Reyes, Claudio Rios-Velasco
Viruses.2025; 17(5): 674. CrossRef - Biological and molecular characterization of Spodoptera littoralis granulovirus with insecticidal activity against the cotton leafworm, Spodoptera littoralis (Boisd.)
Wael Elmenofy, Norhan Yasser, Lamis Gomaa, Hossam S. El-Beltagi, Ahmed Mahmoud Ismail
BioControl.2025;[Epub] CrossRef - Tracking arboviruses, their transmission vectors and potential hosts by nanopore sequencing of mosquitoes
Jeremy D. Mirza, Lilian de Oliveira Guimarães, Sam Wilkinson, Esmenia C. Rocha, Mayara Bertanhe, Vanessa Christe Helfstein, Juliana Telles de-Deus, Ingra M. Claro, Nicola Cumley, Joshua Quick, Nuno R. Faria, Ester C. Sabino, Karin Kirchgatter, Nicholas J.
Microbial Genomics
.2024;[Epub] CrossRef - Morphological, Biological, and Molecular Characterization of Type I Granuloviruses of Spodoptera frugiperda
Magali Ordóñez-García, Juan Carlos Bustillos-Rodríguez, José de Jesús Ornelas-Paz, Carlos Horacio Acosta-Muñiz, Miguel Ángel Salas-Marina, Octavio Jhonathan Cambero-Campos, Mario Orlando Estrada-Virgen, Mario Alberto Morales-Ovando, Claudio Rios-Velasco
Neotropical Entomology.2024; 53(4): 917. CrossRef - Regulatory Mechanisms, Protein Expression and Biological Activity of Photolyase Gene from Spodoptera littoralis Granulovirus Genome
Wael Elmenofy, Lamiaa El-Gaied, Reda Salem, Lamis Gomaa, Alshimaa Mahmoud, Aml Magdy, Ismail Mohamed
Molecular Biotechnology.2023; 65(3): 433. CrossRef - Genome sequence analysis and organization of the Hyphantria cunea granulovirus (HycuGV-Hc1) from Turkey
Donus Gencer, Zeynep Bayramoglu, Remziye Nalcacioglu, Zihni Demirbag, Ismail Demir
Genomics.2020; 112(1): 459. CrossRef - BmNPV infection correlates with the enhancement of the resistance of Bombyx mori cells to UV radiation
Qi Tang, Fangying Chen, Peng Wu, Lipeng Qiu, Huiqing Chen, Keping Chen, Guohui Li
Archives of Insect Biochemistry and Physiology.2019;[Epub] CrossRef - Evidence for the role of BmNPV Bm65 protein in the repair of ultraviolet-induced DNA damage
Qi Tang, Peng Wu, Zhaoyang Hu, Yanhua Yang, Lipeng Qiu, Hanqing Liu, Shanying Zhu, Zhongjian Guo, Hengchuan Xia, Keping Chen, Guohui Li
Journal of Invertebrate Pathology.2017; 149: 82. CrossRef - Molecular and in Silico Characterization of Achaea janata Granulovirus Granulin Gene
Pola Naveen Kumar, Yenumula Gerard Prasad, Mathyam Prabhakar, Arun K. Shanker, Divya Bhanu
Interdisciplinary Sciences: Computational Life Sciences.2017; 9(4): 528. CrossRef - Genome of Cnaphalocrocis medinalis Granulovirus, the First Crambidae-Infecting Betabaculovirus Isolated from Rice Leaffolder to Sequenced
Guangjie Han, Jian Xu, Qin Liu, Chuanming Li, Hongxing Xu, Zhongxian Lu, Qianjun Li
PLOS ONE.2016; 11(2): e0147882. CrossRef - Reaching the melting point: Degradative enzymes and protease inhibitors involved in baculovirus infection and dissemination
Egide Ishimwe, Jeffrey J. Hodgson, Rollie J. Clem, A. Lorena Passarelli
Virology.2015; 479-480: 637. CrossRef - The Complete Sequence of the First Spodoptera frugiperda Betabaculovirus Genome: A Natural Multiple Recombinant Virus
Paola Cuartas, Gloria Barrera, Mariano Belaich, Emiliano Barreto, Pablo Ghiringhelli, Laura Villamizar
Viruses.2015; 7(1): 394. CrossRef - The genome sequence of Pseudoplusia includens single nucleopolyhedrovirus and an analysis of p26 gene evolution in the baculoviruses
Saluana R Craveiro, Peter W Inglis, Roberto C Togawa, Priscila Grynberg, Fernando L Melo, Zilda Maria A Ribeiro, Bergmann M Ribeiro, Sônia N Báo, Maria Elita B Castro
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Paola Cuartas, Gloria Barrera, Emiliano Barreto, Laura Villamizar
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Gloria Patricia Barrera Cubillos, Juliana Gómez, Paola Cuartas, Guillermo León, Laura Fernanda Villamizar Rivero
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Saluana R. Craveiro, Fernando L. Melo, Zilda Maria A. Ribeiro, Bergmann M. Ribeiro, Sônia Nair Báo, Peter W. Inglis, Maria Elita B. Castro
Journal of Invertebrate Pathology.2013; 114(3): 258. CrossRef - Molecular and immunohistochemical characterization of granulin gene encoded in Pieris rapae granulovirus genome
Seunghan Oh, Dong Hyun Kim, Bharat Bhusan Patnaik, Yong Hun Jo, Mi Young Noh, Hyo Jeong Lee, Kwang Ho Lee, Kwang Ho Yoon, Wan-Jong Kim, Ju Young Noh, Heon Cheon Jeong, Yong Seok Lee, Chuan-Xi Zhang, Iksoo Kim, Yeon Soo Han
Journal of Invertebrate Pathology.2013; 113(1): 7. CrossRef - Genome of Epinotia aporema granulovirus (EpapGV), a polyorganotropic fast killing betabaculovirus with a novel thymidylate kinase gene
María Leticia Ferrelli, Ricardo Salvador, Marina Elizabeth Biedma, Marcelo Facundo Berretta, Santiago Haase, Alicia Sciocco-Cap, Pablo Daniel Ghiringhelli, Víctor Romanowski
BMC Genomics.2012;[Epub] CrossRef - Effects of the Spodoptera littoralis granulovirus on the development and reproduction of cotton leafworm S. littoralis
Adel El-Sayed Hatem, Hani Kasim Aldebis, Enrique Vargas Osuna
Biological Control.2011; 59(2): 192. CrossRef - Genomic Sequence Analysis of Granulovirus Isolated from the Tobacco Cutworm, Spodoptera litura
Yong Wang, Jae Young Choi, Jong Yul Roh, Qin Liu, Xue Ying Tao, Jong Bin Park, Jae Su Kim, Yeon Ho Je, Darren P. Martin
PLoS ONE.2011; 6(11): e28163. CrossRef - Baculovirus: Molecular Insights on Their Diversity and Conservation
Solange Ana Belen Miele, Matías Javier Garavaglia, Mariano Nicolás Belaich, Pablo Daniel Ghiringhelli
International Journal of Evolutionary Biology.2011; 2011: 1. CrossRef - Baculovirus cyclobutane pyrimidine dimer photolyases show a close relationship with lepidopteran host homologues
M. A. Biernat, V. I. D. Ros, J. M. Vlak, M. M. van Oers
Insect Molecular Biology.2011; 20(4): 457. CrossRef - Complete Sequence, Analysis and Organization of the Orgyia leucostigma Nucleopolyhedrovirus Genome
David K. Thumbi, Robert J. M. Eveleigh, Christopher J. Lucarotti, Renée Lapointe, Robert I. Graham, Lillian Pavlik, Hilary A. M. Lauzon, Basil M. Arif
Viruses.2011; 3(11): 2301. CrossRef - DNA photolyases of Chrysodeixis chalcites nucleopolyhedrovirus are targeted to the nucleus and interact with chromosomes and mitotic spindle structures
F. Xu, J. M. Vlak, A. P. M. Eker, M. M. van Oers
Journal of General Virology.2010; 91(4): 907. CrossRef
- Molecular Characterization of ORFs 2 to 7 of Korean Porcine Reproductive and Respiratory Syndrome Virus (CA) and Its Protein Expression by Recombinant Baculoviruses
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Hyun Na Koo , Jeong Mi Oh , Jae Kyung Lee , Jae Young Choi , Kwang Sik Lee , Jong Yul Roh , Yeon Ho Je , Byung Rae Jin , Sung Sik Yoo , Jae Su Kim , Young In Kim , In Joong Yoon , Soo Dong Woo
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J. Microbiol. 2008;46(6):709-719. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0224-x
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To determine the characteristics of the Korean porcine reproductive and respiratory syndrome virus (PRRSV), CA, which was isolated from the serum of an infected pig in 2006, we investigated the nucleotide sequence and expression of the structural ORFs (ORFs 2 to 7) using the bApGOZA system. We found that the structural ORFs 2 to 7 of CA consisted of 3188 nucleotides that were the same as those formed from VR-2332. Comparison of the CA with the other strains revealed nucleotide sequence identity ranging from 89.8 to 99.5%. To better understand the genetic relationships between other strains, phylogenetic analyses were performed. The CA strain was closely related to the other North American genotype strains but formed a distinct branch with high bootstrap support. Additionally, expression levels of the PRRSV proteins in insect cells were strong or partially weak. The results of this study have implications for both the taxonomy of PRRSV and vaccine development.
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- Geographic distribution and molecular analysis of porcine reproductive and respiratory syndrome viruses circulating in swine farms in the Republic of Korea between 2013 and 2016
Hyeonjeong Kang, Ji Eun Yu, Ji-Eun Shin, Areum Kang, Won-Il Kim, Changhee Lee, Jienny Lee, In-Soo Cho, Se-Eun Choe, Sang-Ho Cha
BMC Veterinary Research.2018;[Epub] CrossRef - Enhanced expression of the structural protein of porcine reproductive and respiratory syndrome virus (PRRSV) by SUMO fusion
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Journal Article
- Proteomic Analysis of Outer Membrane Proteins from Acinetobacter baumannii DU202 in Tetracycline Stress Condition
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Sung-Ho Yun , Chi-Won Choi , Soon-Ho Park , Je Chul Lee , Sun-Hee Leem , Jong-Soon Choi , Soohyun Kim , Seung Il Kim
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J. Microbiol. 2008;46(6):720-727. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0202-3
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Abstract
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Acinetobacter baumannii readily developed antimicrobial resistance to clinically available antibiotics. A. baumannii DU202 is a multi-drug resistant strain, and is highly resistant to tetracycline (MIC>1,024μg/ml). The surface proteome of A. baumannii DU202 in response to the sub-minimal inhibitory concentration (subMIC) of tetracycline was analyzed by 2-DE/MS-MS and 1-DE/LC/MS-MS to understand the pathways that form barriers for tetracycline. Membrane expression of major outer membrane proteins (Omps) was significantly decreased in response to the subMIC of tetracycline. These Omps with sizes of 38, 32, 28, and 21 kDa were identified as OmpA38, OmpA32, CarO, and OmpW, respectively. However, transcription level of these Omps was not significantly changed. 1-DE/LC/MS-MS analysis of secreted proteins showed that OmpA38, CarO, OmpW, and other Omps were increasingly secreted at tetracycline condition. This result suggests that A. baumannii actively regulates the membrane expression and the secretion of Omps to overcome antibiotic stress condition.
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- Enhancement of Immunotherapeutic Effects of HPV16E7 on Cervical Cancer by Fusion with CTLA4 Extracellular Region
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Yi Zheng , Yijuan Zhang , Jun Wan , Chaofan Shi , Laiqiang Huang
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J. Microbiol. 2008;46(6):728-736. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0087-1
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Cervical cancer is caused by infection by high-risk human papillomavirus (HPV), especially HPV16. Limitations in current treatments of cervical cancers call for the development of new and improved immunotherapies. This study aims at investigating the efficacy of a novel vaccine consisting of modified HPV 16E7 fused with human cytotoxic T-lymphocyte antigen 4 (CTLA4). The regions in HPV16 E7 gene associated with its transformation and CTL-enhanced response were modified; the resultant HPV16mE7 was fused with extracellular region of CTLA4 to generate HPVm16E7-eCTLA4 fusion protein. Binding of this fusion protein to B7 molecules expressed on antigen presenting-cells (APCs) was demonstrated. C57BL/6 (H-2b) mice immunized with low dose of the fusion protein (10 μg) produced higher titer antibody and stronger specific CTL response, and expressed higher levels of IFN-γ and IL-12, compared with those immunized with HPVm16E7 only or admixture of HPVm16E7 and CTLA4, or PBS; and were protected from lethal dose tumor challenge. Tumor growth was retarded and survival prolonged in mouse models with the fusion protein treatment. Our results demonstrate that fusion of HPV16 E7 with eCTLA4 targeting APCs resulted in enhanced immunity, and that this fusion protein may be useful for improving the efficacy of immunotherapeutic treatments of cervical cancer and other HPV16 infection-associated tumors.
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Jong Ro Kim , Dool-Ri Oh , Mi Hye Cha , Byoung Sik Pyo , Joon Haeng Rhee , Hyon E. Choy , Won Keun Oh , Young Ran Kim
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J. Microbiol. 2008;46(6):737-743. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0232-x
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Vibrio vulnificus, a good model organism of bacterial septicemia, causes fatal septicemia manifesting a fulminating course and a high mortality rate within days. In order to identify new natural substances preventing V. vulnificus infection, a plant library was screened for inhibiting cytotoxicity to host cells by using Trypan blue staining and LDH assay. We found that Polygoni Cuspidati Radix potently suppressed the acute death of HeLa and RAW264.7 cells in a dose dependent manner. Further studies revealed that Polygoni Cuspidati Radix inhibited V. vulnificus growth and survival in HI broth and seawater, respectively. We confirmed that Polygoni Cuspidati Radix contained high level of emodin by thin layer chromatography (TLC). Emodin showed direct antibacterial activity against V. vulnificus. In addition, emodin prevented the morphologic damages and acute death of HeLa cells caused from V. vulnificus. The safety of Polygoni Cuspidati Radix and emodin to host cells was confirmed by MTT assay. Polygoni Cuspidati Radix and emodin protected mice from V. vulnificus infection.
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Journal Article
- Inhibitory Effect of Methyl Gallate and Gallic Acid on Oral Bacteria
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Mi-Sun Kang , Jong-Suk Oh , In-Chol Kang , Suk-Jin Hong , Choong-Ho Choi
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J. Microbiol. 2008;46(6):744-750. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0235-7
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Abstract
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This study examined the ability of methyl gallate (MG) and gallic acid (GA), the main compounds of gallotannins in Galla Rhois, to inhibit the proliferation of oral bacterial and the in vitro formation of Streptococcus mutans biofilms. The antimicrobial activities of these compounds were evaluated in vitro using the broth microdilution method and a beaker-wire test. Both MG and GA had inhibitory effects on the growth of cariogenic (MIC<8 mg/ml) and periodontopathic bacteria (MIC=1 mg/ml). Moreover, these compounds significantly inhibited the in vitro formation of S. mutans biofilms (MG, 1 mg/ml; GA, 4 mg/ml; P<0.05). MG was more effective in inhibiting bacterial growth and the formation of S. mutans biofilm than GA. In conclusion, MG and GA can inhibit the growth of oral pathogens and S. mutans biofilm formation, and may be used to prevent the formation of oral biofilms.
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