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Volume 50(5); October 2012
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Review
REVIEW] The Role of Type III Secretion System 2 in Vibrio parahaemolyticus Pathogenicity
Hyeilin Ham , Kim Orth
J. Microbiol. 2012;50(5):719-725.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2550-2
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AbstractAbstract
Vibrio parahaemolyticus, a Gram-negative marine bacterial pathogen, is emerging as a major cause of food-borne illnesses worldwide due to the consumption of raw seafood leading to diseases including gastroenteritis, wound infection, and septicemia. The bacteria utilize toxins and type III secretion system (T3SS) to trigger virulence. T3SS is a multi-subunit needle-like apparatus used to deliver bacterial proteins, termed effectors, into the host cytoplasm which then target various eukaryotic signaling pathways. V. parahaemolyticus carries two T3SSs in each of its two chromosomes, named T3SS1 and T3SS2, both of which play crucial yet distinct roles during infection: T3SS1 causes cytotoxicity whereas T3SS2 is mainly associated with enterotoxicity. Each T3SS secretes a unique set of effectors that contribute to virulence by acting on different host targets and serving different functions. Emerging studies on T3SS2 of V. parahaemolyticus, reveal its regulation, translocation, discovery, characterization of its effectors, and development of animal models to understand the enterotoxicity. This review on recent findings for T3SS2 of V. parahaemolyticus highlights a novel mechanism of invasion that appears to be conserved by other marine bacteria.

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Research Support, Non-U.S. Gov'ts
Reliability of Non-Culturable Virus Monitoring by PCR-Based Detection Methods in Environmental Waters Containing Various Concentrations of Target RNA
Eung Seo Koo , Chang-Hoon Yoo , Youjin Na , Soo Young Park , Hey Rhyoung Lyoo , Yong Seok Jeong
J. Microbiol. 2012;50(5):726-734.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2279-y
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AbstractAbstract
Owing to the lack of practical cell culture system for human noroviruses (HuNoV), various detection methods based on conventional reverse transcription-PCR (RT-PCR) and the quantitative real-time PCR have been major tools for monitoring environmental water safety. In this study, we showed that the proportion of water sample concentrates used for one-step RT-PCR significantly influences false-negative findings of the non-culturable viruses. In total, 59 archived samples of previously analyzed water concentrates were reexamined for HuNoV RNA by the one-step RT-PCR and semi-nested PCR. Using new aliquots for RNA extraction for every trial, up to 20 PCR trials were performed for each archive to determine whether the crosscheck results supported the previous determinations. We reconfirmed that 27.6% (8/29) of the samples were HuNoV-positive samples: 6.7% (1/15) from groundwater, 33.3% (3/9) from river water, and 80% (4/5) from treated sewage effluent (TSE). These results corresponded to the ratio of previously negative HuNoV samples now identified as positive (8/30): 6.7% (1/15) from groundwater, 20% (1/5) from river water, and 60% (6/10) from TSE. To elucidate the cause of these results, 16 different concentrations of murine norovirus (MNV) RNA (from 2×102 to 8×103 copies, divided into 10 tubes for each concentration) were subjected to one-step RT-PCR. The detection frequency and reproducibility decreased sharply when the number of MNV RNA copies fell below threshold levels. These observations suggest that the proportion of water concentrate used for PCR-based detection should be considered carefully when deciding viral presence in certain types of environmental water, particularly in regard with legal controls.
Pyrosequencing Analysis of the Bacterial Communities in the Guts of Honey Bees Apis cerana and Apis mellifera in Korea
Jae-Hyung Ahn , In-Pyo Hong , Jeung-Im Bok , Byung-Yong Kim , Jaekyeong Song , Hang-Yeon Weon
J. Microbiol. 2012;50(5):735-745.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2188-0
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AbstractAbstract
The bacterial communities in the guts of the adults and larvae of the Asian honey bee Apis cerana and the European honey bee Apis mellifera were surveyed by pyrosequencing the 16S rRNA genes. Most of the gut bacterial 16S rRNA gene sequences were highly similar to the known honey bee-specific ones and affiliated with Pasteurellaceae or lactic acid bacteria (LAB). The numbers of operational taxonomic units (OTUs, defined at 97% similarity) were lower in the larval guts (6 or 9) than in the adult guts (18 or 20), and the frequencies of Pasteurellaceae-related OTUs were higher in the larval guts while those of LAB-related OTUs in the adult guts. The frequencies of Lactococcus, Bartonella, Spiroplasma, Enterobacteriaceae, and Flavobacteriaceae-related OTUs were much higher in A. cerana guts while Bifidobacterium and Lachnospiraceae-related OTUs were more abundant in A. mellfera guts. The bacterial community structures in the midguts and hindguts of the adult honey bees were not different for A. cerana, but significantly different for A. mellifera. The above results substantiated the previous observation that honey bee guts are dominated by several specific bacterial groups, and also showed that the relative abundances of OTUs could be markedly changed depending on the developmental stage, the location within the gut, and the honey bee species. The possibility of using the gut bacterial community as an indicator of honey bee health was discussed.

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Journal Article
Comparative Study of Olive Oil Mill Wastewater Treatment Using Free and Immobilized Coriolopsis polyzona and Pycnoporus coccineus
Mohamed Neifar , Atef Jaouani , María Jesús Martínez , Michel J. Penninckx
J. Microbiol. 2012;50(5):746-753.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2079-4
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AbstractAbstract
The efficiency of the two white-rot fungi Pycnoporus coccineus and Coriolopsis polyzona in the Olive Oil Mill Wastewater (OOMW) treatment was investigated. Both fungi were active in the decolourisation and COD removal of OOMW at 50 g/L COD, but only the first fungus remains effective on the crude effluent (COD=100 g/L). Moreover P. coccineus was less affected by oxygen supplementation and exhibited a high tolerance to agitation in comparison to C. polyzona. However, it required a nitrogen supplementation to obtain faster and higher COD removal. To overcome the negative effect of agitation on fungi growth and efficiency, immobilisation of C. polyzona and P. coccineus in polyurethane foam was applied. The immobilized system showed better COD decreases during three consecutive batches without remarkable loss of performances. The results obtained in this study suggested that immobilized C. polyzona and especially immobilized P. coccineus might be applicable to a large scale for the removal colour and COD of OOMW.

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Research Support, Non-U.S. Gov'ts
Characterization of the Bacterial and Archaeal Communities in Rice Field Soils Subjected to Long-Term Fertilization Practices
Jae-Hyung Ahn , Jaekyeong Song , Byung-Yong Kim , Myung-Sook Kim , Jae-Ho Joa , Hang-Yeon Weon
J. Microbiol. 2012;50(5):754-765.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2409-6
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  • 115 Scopus
AbstractAbstract
The bacterial and archaeal communities in rice field soils subjected to different fertilization regimes for 57 years were investigated in two different seasons, a non-planted, drained season (April) and a rice-growing, flooded season (August), by performing soil dehydrogenase assay, real-time PCR assay and pyrosequencing analysis. All fertilization regimes increased the soil dehydrogenase activity while the abundances of bacteria and archaea increased in the plots receiving inorganic fertilizers plus compost and not in those receiving inorganic fertilizers only. Rice-growing and flooding decreased the soil dehydrogenase activity while they increased the bacterial diversity in rice field soils. The bacterial communities were dominated by Chloroflexi, Proteobacteria, and Actinobacteria and the archaeal communities by Crenarchaeota at the phylum level. In principal coordinates analysis based on the weighted Fast UniFrac metric, the bacterial and archaeal communities were separated primarily by season, and generally distributed along with soil pH, the variation of which had been caused by long-term fertilization. Variations in the relative abundance according to the season or soil pH were observed for many bacterial and archaeal groups. In conclusion, the microbial activity, prokaryotic abundance and diversity, and prokaryotic community structure in the rice field soils were changed by season and long-term fertilization.
PyroTrimmer: a Software with GUI for Pre-Processing 454 Amplicon Sequences
Jeongsu Oh , Byung Kwon Kim , Wan-Sup Cho , Soon Gyu Hong , Kyung Mo Kim
J. Microbiol. 2012;50(5):766-769.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2494-6
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AbstractAbstract
The ultimate goal of metagenome research projects is to understand the ecological roles and physiological functions of the microbial communities in a given natural environment. The 454 pyrosequencing platform produces the longest reads among the most widely used next generation sequencing platforms. Since the relatively longer reads of the 454 platform provide more information for identification of microbial sequences, this platform is dedicated to microbial community and population studies. In order to accurately perform the downstream analysis of the 454 multiplex datasets, it is necessary to remove artificially designed sequences located at either ends of individual reads and to correct lowquality sequences. We have developed a program called Pyro-Trimmer that removes the barcodes, linkers, and primers, trims sequence regions with low quality scores, and filters out low-quality sequence reads. Although these functions have previously been implemented in other programs as well, PyroTrimmer has novelty in terms of the following features: i) more sensitive primer detection using Levenstein distance and global pairwise alignment, ii) the first stand-alone software with a graphic user interface, and iii) various options for trimming and filtering out the low-quality sequence reads. PyroTrimmer, written in JAVA, is compatible with multiple operating systems and can be downloaded free at http://pyrotrimmer.kobic.re.kr.

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    Yung Mi Lee, Doshik Hahm, You-Jung Jung, Sung Hyun Park, Jongsik Chun, Soon Gyu Hong
    Polar Biology.2014; 37(4): 587.     CrossRef
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    Ok-Hwa Hwang, Sebastian Raveendar, Young-Ju Kim, Ji-Hun Kim, Tae-Hun Kim, Dong-Yoon Choi, Che Ok Jeon, Sung-Back Cho, Kyung-Tai Lee
    Journal of Microbiology.2014; 52(11): 918.     CrossRef
  • Effect of long-term different fertilization on bacterial community structures and diversity in citrus orchard soil of volcanic ash
    Jae Ho Joa, Hang Yeon Weon, Hae Nam Hyun, Young Chull Jeun, Sang Wook Koh
    Journal of Microbiology.2014; 52(12): 995.     CrossRef
  • A metagenomics‐based approach to the top‐down effect on the detritivore food web: a salamanders influence on fungal communities within a deciduous forest
    Donald M. Walker, Brandy R. Lawrence, Dakota Esterline, Sean P. Graham, Michael A. Edelbrock, Jessica A. Wooten
    Ecology and Evolution.2014; 4(21): 4106.     CrossRef
  • Differences in Gastric Mucosal Microbiota Profiling in Patients with Chronic Gastritis, Intestinal Metaplasia, and Gastric Cancer Using Pyrosequencing Methods
    Chang Soo Eun, Byung Kwon Kim, Dong Soo Han, Seon Young Kim, Kyung Mo Kim, Bo Youl Choi, Kyu Sang Song, Yong Sung Kim, Jihyun F. Kim
    Helicobacter.2014; 19(6): 407.     CrossRef
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    Ok-Sun Kim, Namyi Chae, Hyun Soo Lim, Ahnna Cho, Jeong Hoon Kim, Soon Gyu Hong, Jeongsu Oh
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Functional Characterization of the Genes tauO, tauK, and tauI in the Biosynthesis of Tautomycetin
Fen Wang , Rixiang Kong , Bo Liu , Jing Zhao , Rongguo Qiu , Li Tang
J. Microbiol. 2012;50(5):770-776.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2154-x
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AbstractAbstract
Tautomycetin is a specific protein phosphatase I inhibitor. In an effort to elucidate the biosynthetic mechanism of tautomycetin, we inactivated genes of the tautomycetin biosynthetic gene cluster, tauI, tauO, and tauK, which encode for putative P450 oxidase, citryl-CoA lyase, and esterase enzymes, respectively. The mutant STQ0606 (ΔtauO) did not produce any detectable amount of tautomycetin intermediates but could convert dialkylmaleic anhydride to tautomycetin, strongly indicating that TauO was involved in dialkylmaleic anhydride biosynthesis. STQ1211 (ΔtauK) accumulated dialkylmaleic anhydride, whereas the cofermentation of STQ1211 (ΔtauK) and STQ0606 (ΔtauO) restored the production of tautomycetin. Together, these results suggest that TauK was responsible for the conjugation of dialkylmaleic anhydride and the polyketide moiety in tautomycetin biosynthesis. The disruption of tauI resulted in the accumulation of 5-des-ketotautomycetin, revealing that TauI was responsible for the oxidation at C5 as the last step. Although the shunt pathways were involved in the biosynthesis of tautomycetin, the main post-polyketide synthase tailoring steps were dehydration, decarboxylation and oxidation, taking place consecutively. This study allowed us to predict the biosynthesis of tautomycetin more accurately and provided novel insights into the mechanism of the biosynthesis of tautomycetin.

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  • Biocatalytic role of cytochrome P450s to produce antibiotics: A review
    Anup Adhikari, Sajan Shakya, Shreesti Shrestha, Dipa Aryal, Kavi Prasad Timalsina, Dipesh Dhakal, Yogan Khatri, Niranjan Parajuli
    Biotechnology and Bioengineering.2023; 120(12): 3465.     CrossRef
  • Biosynthesis, regulation, and engineering of a linear polyketide tautomycetin: a novel immunosuppressant in Streptomyces sp. CK4412
    Si-Sun Choi, Hee-Ju Nah, Hye-rim Pyeon, Eung-Soo Kim
    Journal of Industrial Microbiology and Biotechnology.2017; 44(4-5): 555.     CrossRef
  • Optimized transformation of Streptomyces sp. ATCC 39366 producing leptomycin by electroporation
    Yong-Qiang Fan, Hong-Jian Liu, Li Yan, Yu-Shi Luan, Hai-Meng Zhou, Jun-Mo Yang, Shang-Jun Yin, Yu-Long Wang
    Journal of Microbiology.2013; 51(3): 318.     CrossRef
  • A Novel Alkaloid from Marine-Derived Actinomycete Streptomyces xinghaiensis with Broad-Spectrum Antibacterial and Cytotoxic Activities
    Wence Jiao, Fenghua Zhang, Xinqing Zhao, Jiehan Hu, Joo-Won Suh, Marie-Joelle Virolle
    PLoS ONE.2013; 8(10): e75994.     CrossRef
Identification and Characterization of an Autolysin Gene, atlA, from Streptococcus criceti
Haruki Tamura , Arisa Yamada , Hirohisa Kato
J. Microbiol. 2012;50(5):777-784.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2187-1
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AbstractAbstract
AtlA of Streptococcus mutans is a major autolysin and belongs to glycoside hydrolase family 25 with cellosyl of Streptomyces coelicolor. The autolysin gene (atlA) encoding AtlA was identified from S. criceti. AtlA of S. criceti comprises the signal sequence in the N-terminus, the putative cell-wallbinding domain in the middle, and the catalytic domain in the C-terminus. Homology modeling analysis of the catalytic domain of AtlA showed the resemblance of the spatial arrangement of five amino acids around the predicted catalytic cavity to that of cellosyl. Recombinant AtlA and its four point mutants, D655A, D747A, W831A, and D849A, were evaluated on zymogram of S. criceti cells. Lytic activity was destroyed in the mutants D655A and D747A and diminished in the mutants W831A and D849A. These results suggest that Asp655 and Asp747 residues are critical for lytic activity and Trp831 and Asp849 residues are also associated with enzymatic activity.
Genome-Wide Enrichment Screening Reveals Multiple Targets and Resistance Genes for Triclosan in Escherichia coli
Byung Jo Yu , Jung Ae Kim , Hyun Mok Ju , Soo-Kyung Choi , Seung Jin Hwang , Sungyoo Park , EuiJoong Kim , Jae-Gu Pan
J. Microbiol. 2012;50(5):785-791.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2439-0
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AbstractAbstract
Triclosan is a widely used biocide effective against different microorganisms. At bactericidal concentrations, triclosan appears to affect multiple targets, while at bacteriostatic concentrations, triclosan targets FabI. The site-specific antibioticlike mode-of-action and a widespread use of triclosan in household products claimed to possibly induce cross-resistance to other antibiotics. Thus, we set out to define more systematically the genes conferring resistance to triclosan; A genomic library of Escherichia coli strain W3110 was constructed and enriched in a selective medium containing a lethal concentration of triclosan. The genes enabling growth in the presence of triclosan were identified by using a DNA microarray and confirmed consequently by ASKA clones overexpressing the selected 62 candidate genes. Among these, forty-seven genes were further confirmed to enhance the resistance to triclosan; these genes, including the FabI target, were involved in inner or outer membrane synthesis, cellsurface material synthesis, transcriptional activation, sugar phosphotransferase (PTS) systems, various transporter systems, cell division, and ATPase and reductase/dehydrogenase reactions. In particular, overexpression of pgsA, rcsA, or gapC conferred to E. coli cells a similar level of triclosan resistance induced by fabI overexpression. These results indicate that triclosan may have multiple targets other than well-known FabI and that there are several undefined novel mechanisms for the resistance development to triclosan, thus probably inducing cross antibiotic resistance.

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  • Family Sphingomonadaceae as the key executor of triclosan degradation in both nitrification and denitrification systems
    Huihui Dai, Jingfeng Gao, Dingchang Li, Zhiqi Wang, Yingchao Cui, Yifan Zhao
    Chemical Engineering Journal.2022; 442: 136202.     CrossRef
  • Metagenomics combined with DNA-based stable isotope probing provide comprehensive insights of active triclosan-degrading bacteria in wastewater treatment
    Huihui Dai, Jingfeng Gao, Dingchang Li, Zhiqi Wang, Wanjun Duan
    Journal of Hazardous Materials.2021; 404: 124192.     CrossRef
  • The food-grade antimicrobial xanthorrhizol targets the enoyl-ACP reductase (FabI) in Escherichia coli
    Yogiara, Elena A. Mordukhova, Dooil Kim, Won-Gon Kim, Jae-Kwan Hwang, Jae-Gu Pan
    Bioorganic & Medicinal Chemistry Letters.2020; 30(24): 127651.     CrossRef
  • The key active degrader, metabolic pathway and microbial ecology of triclosan biodegradation in an anoxic/oxic system
    Huihui Dai, Jingfeng Gao, Shijie Wang, Dingchang Li, Zhiqi Wang
    Bioresource Technology.2020; 317: 124014.     CrossRef
  • Biocide Exposure Induces Changes in Susceptibility, Pathogenicity, and Biofilm Formation in Uropathogenic Escherichia coli
    E. L. Henly, J. A. R. Dowling, J. B. Maingay, M. M. Lacey, T. J. Smith, S. Forbes
    Antimicrobial Agents and Chemotherapy.2019;[Epub]     CrossRef
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    Carl J. Balibar, Terry Roemer
    Briefings in Functional Genomics.2016; 15(2): 147.     CrossRef
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    Rebecca Wesgate, Pierre Grasha, Jean-Yves Maillard
    American Journal of Infection Control.2016; 44(4): 458.     CrossRef
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    Tânia Curiao, Emmanuela Marchi, Denis Grandgirard, Ricardo León-Sampedro, Carlo Viti, Stephen L. Leib, Fernando Baquero, Marco R. Oggioni, José Luis Martinez, Teresa M. Coque
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    Malathy Krishnamurthy, Richard T. Moore, Sathish Rajamani, Rekha G. Panchal
    BMC Microbiology.2016;[Epub]     CrossRef
  • Escherichia coli ASKA Clone Library Harboring tRNA-Specific Adenosine Deaminase (tadA) Reveals Resistance towards Xanthorrhizol
    Yogiara, Dooil Kim, Jae-Kwan Hwang, Jae-Gu Pan
    Molecules.2015; 20(9): 16290.     CrossRef
  • Development of a Protocol for Predicting Bacterial Resistance to Microbicides
    Laura Knapp, Alejandro Amézquita, Peter McClure, Sara Stewart, Jean-Yves Maillard, G. T. Macfarlane
    Applied and Environmental Microbiology.2015; 81(8): 2652.     CrossRef
  • Mutations upstream of fabI in triclosan resistant Staphylococcus aureus strains are associated with elevated fabI gene expression
    Denis Grandgirard, Leonardo Furi, Maria Laura Ciusa, Lucilla Baldassarri, Daniel R Knight, Ian Morrissey, Carlo R Largiadèr, Stephen L Leib, Marco R Oggioni
    BMC Genomics.2015;[Epub]     CrossRef
  • Polymorphic Variation in Susceptibility and Metabolism of Triclosan-Resistant Mutants of Escherichia coli and Klebsiella pneumoniae Clinical Strains Obtained after Exposure to Biocides and Antibiotics
    Tânia Curiao, Emmanuela Marchi, Carlo Viti, Marco R. Oggioni, Fernando Baquero, José Luis Martinez, Teresa M. Coque
    Antimicrobial Agents and Chemotherapy.2015; 59(6): 3413.     CrossRef
  • The impact of triclosan on the spread of antibiotic resistance in the environment
    Daniel E. Carey, Patrick J. McNamara
    Frontiers in Microbiology.2015;[Epub]     CrossRef
  • Triclosan-Induced Aminoglycoside-Tolerant Listeria monocytogenes Isolates Can Appear as Small-Colony Variants
    Vicky G. Kastbjerg, Line Hein-Kristensen, Lone Gram
    Antimicrobial Agents and Chemotherapy.2014; 58(6): 3124.     CrossRef
  • Recent advances in the potential interconnection between antimicrobial resistance to biocides and antibiotics
    Marco R Oggioni, Leonardo Furi, Joana R Coelho, Jean-Yves Maillard, José L Martínez
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Characterization of Thermostable Deblocking Aminopeptidases of Archaeon Thermococcus onnurineus NA1 by Proteomic and Biochemical Approaches
Yeol Gyun Lee , Sun-Hee Leem , Young-Ho Chung , Seung Il Kim
J. Microbiol. 2012;50(5):792-797.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2461-2
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AbstractAbstract
Thermococcus onnurineus NA1 is a hyperthermophilic archaeon that grows optimally at >80°C. The deblocking aminopeptidase (DAP) (TNA1-DAP1) encoded in Ton_1032 of T. onnurineus NA1 is considered a major DAP. However, four genes encoding putative DAP have been identified from a genomic analysis of T. onnurineus NA1. A proteomic analysis revealed that all four DAPs were differentially induced in YPS culture medium and, particularly, two DAPs (TNA1-DAP1 and TNA1-DAP2) were dominantly expressed in T. onnurineus NA1. The biochemical properties and enzyme activity of DAPs induced in an E. coli expression system suggested that the two major DAPs play complementary roles in T. onnurineus NA1.

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  • Proteomic Insights into Sulfur Metabolism in the Hydrogen-Producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1
    Yoon-Jung Moon, Joseph Kwon, Sung-Ho Yun, Hye Lim, Jonghyun Kim, Soo Kim, Sung Kang, Jung-Hyun Lee, Seung Kim, Young-Ho Chung
    International Journal of Molecular Sciences.2015; 16(5): 9167.     CrossRef
  • Characterization of Streptococcus pneumoniae N-acetylglucosamine-6-phosphate deacetylase as a novel diagnostic marker
    Chi-Won Choi, Hee-Young An, Yong Ju Lee, Yeol Gyun Lee, Sung Ho Yun, Edmond Changkyun Park, Yeonhee Hong, Gun-Hwa Kim, Jae-Eun Park, Sun Jong Baek, Hyun Sik Kim, Seung Il Kim
    Journal of Microbiology.2013; 51(5): 659.     CrossRef
Selection of a Streptomyces Strain Able to Produce Cell Wall Degrading Enzymes and Active against Sclerotinia sclerotiorum
Adriana Fróes , Andrew Macrae , Juliana Rosa , Marcella Franco , Rodrigo Souza , Rosângela Soares , Rosalie Coelho
J. Microbiol. 2012;50(5):798-806.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2060-2
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AbstractAbstract
Control of plant pathogen Sclerotinia sclerotiorum is an ongoing challenge because of its wide host range and the persistence of its sclerotia in soil. Fungicides are the most commonly used method to control this fungus but these can have ecotoxicity impacts. Chitinolytic Streptomyces strains isolated from Brazilian tropical soils were capable of inhibiting S. sclerotiorum growth in vitro, offering new possibilities for integrated pest management and biocontrol, with a new approach to dealing with an old problem. Strain Streptomyces sp. 80 was capable of irreversibly inhibiting fungal growth. Compared to other strains, its crude enzymes had the highest chitinolytic levels when measured at 25°C and strongly inhibited sclerotia from S. sclerotiorum. It produced four hydrolytic enzymes involved in fungal cell wall degradation when cultured in presence of the fungal mycelium. The best production, obtained after three days, was 0.75 U/ml for exochitinase, 0.9 U/ml for endochitinase, 0.16 U/ml for glucanase, and 1.78 U/ml for peptidase. Zymogram analysis confirmed two hydrolytic bands of chitinolytic activity with apparent molecular masses of 45.8 and 206.8 kDa. One glucanase activity with an apparent molecular mass of 55 kDa was also recorded, as well as seven bands of peptidase activity with apparent molecular masses ranging from 15.5 to 108.4 kDa. Differential interference contrast microscopy also showed alterations of hyphal morphology after co-culture. Streptomyces sp. 80 seems to be promising as a biocontrol agent against S. sclerotiorum, contributing to the development of new methods for controlling plant diseases and reducing the negative impact of using fungicides.

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    Applied Microbiology and Biotechnology.2024;[Epub]     CrossRef
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    Talwinder Kaur, Kanika Khanna, Sonika Sharma, Rajesh K. Manhas
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  • Exploring the Potentiality of Native Actinobacteria to Combat the Chilli Fruit Rot Pathogens under Post-Harvest Pathosystem
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    Zineb Faiza Boukhatem, Chahinez Merabet, Hassini Tsaki
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    Masafumi Shimizu, Hushna Ara Naznin, Ayaka Hieno
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    New Journal of Chemistry.2022; 46(18): 8592.     CrossRef
  • A Rhizobacterium, Streptomyces albulus Z1-04-02, Displays Antifungal Activity against Sclerotium Rot in Mungbean
    On-Uma Ruangwong, Kaewalin Kunasakdakul, Sompong Chankaew, Kitsada Pitija, Anurag Sunpapao
    Plants.2022; 11(19): 2607.     CrossRef
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    Cathryn A. O’Sullivan, Katharina Belt, Louise F. Thatcher
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  • Overexpression of OsPGIP2 confers Sclerotinia sclerotiorum resistance in Brassica napus through increased activation of defense mechanisms
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    Sasikumar Arunachalam Palaniyandi, Seung Hwan Yang, Lixin Zhang, Joo-Won Suh
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In Vitro Development and Transfer of Resistance to Chlortetracycline in Bacillus subtilis
Menghong Dai , Junjie Lu , Yulian Wang , Zhenli Liu , Zonghui Yuan
J. Microbiol. 2012;50(5):807-812.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-1454-5
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AbstractAbstract
The present criteria and rules controlling the approval of the use of probiotics are limited to antibiotic resistance patterns and the presence of antibiotic resistance genes in bacteria. There is little information available in the literature regarding the risk of the usage of probiotics in the presence of antibiotic pressure. In this study we investigated the development and transfer of antibiotic resistance in Bacillus subtilis selected in vitro by chlortetracycline in a stepwise manner. Bacillus subtilis was exposed to increasing concentrations of chlortetracyclineto induce in vitro resistance to chlortetracycline, and the minimal inhibitory concentrations were determinedfor the mutants. Resistant B. subtilis were conjugated with Escherichia coli NK5449 and Enterococcus faecalis JH2-2 using the filter mating. Three B. subtilis tetracycline resistant mutants (namely, BS-1, BS-2, and BS-3) were derived in vitro. A tetracycline resistant gene, tet (K), was found in the plasmids of BS-1 and BS-2. Three conjugates (BS-1N, BS-2N, and BS-3N) were obtained when the resistant B. subtilis was conjugated with E. coli NK5449. The conjugation frequencies for the BS-1N, BS-2N, and BS-3N conjugates were 4.57×10-7, 1.4×10-7, and 1.3×10-8, respectively. The tet(K) gene was found only in the plasmids of BS-1N. These results indicate that long-term use of probiotics under antibiotic selection pressure could cause antibiotic resistance, and the resistance gene could be transferred to other bacteria. The risk arising from the use of probiotics under antibiotic pressure should be considered in the criteria and rules for the safety assessment of probiotics.

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    Klaudia Zawadzka, Karolina Oszust, Michał Pylak, Agata Gryta, Jacek Panek, Tomasz Lipa, Magdalena Frąc
    International Agrophysics.2025; 39(2): 145.     CrossRef
  • Antibiotic Resistance Gene Expression in Veterinary Probiotics: Two Sides of the Coin
    Ádám Kerek, István Román, Ábel Szabó, Nikolett Palkovicsné Pézsa, Ákos Jerzsele
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  • Probiyotiklerin Genel Özellikleri ve Sağlık Üzerine Etkileri
    Batuhan Horasan, Nevzat Atalay Çelikyürek
    Sağlık Akademisi Kastamonu.2024; 9(2): 345.     CrossRef
  • Tetracyclines resistance in Mycoplasma and Ureaplasma urogenital isolates derived from human: a systematic review and meta-analysis
    Xiaoyan Wen, Mojgan Sarabi Nobakht, Yue Yang, Ebrahim Kouhsari, Sara Hajilari, Matin Zafar Shakourzadeh, Khalil Azizian
    Annals of Clinical Microbiology and Antimicrobials.2023;[Epub]     CrossRef
  • Bacillus Species as Direct-Fed Microbial Antibiotic Alternatives for Monogastric Production
    Shifa A. Bahaddad, Meshal H. K. Almalki, Othman A. Alghamdi, Sayed S. Sohrab, Muhammad Yasir, Esam I. Azhar, Hichem Chouayekh
    Probiotics and Antimicrobial Proteins.2023; 15(1): 1.     CrossRef
  • Bacillusspore-forming probiotics: benefits with concerns?
    Svetoslav Dimitrov Todorov, Iskra Vitanova Ivanova, Igor Popov, Richard Weeks, Michael Leonidas Chikindas
    Critical Reviews in Microbiology.2022; 48(4): 513.     CrossRef
  • Exploring the persistence and spreading of antibiotic resistance from manure to biocompost, soils and vegetables
    Chengjun Pu, Yao Yu, Jianxiong Diao, Xiaoyan Gong, Ji Li, Ying Sun
    Science of The Total Environment.2019; 688: 262.     CrossRef
  • Bacillusprobiotics: an alternative to antibiotics for livestock production
    S. Mingmongkolchai, W. Panbangred
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  • Distinguishing Effects of Ultraviolet Exposure and Chlorination on the Horizontal Transfer of Antibiotic Resistance Genes in Municipal Wastewater
    Mei-Ting Guo, Qing-Bin Yuan, Jian Yang
    Environmental Science & Technology.2015; 49(9): 5771.     CrossRef
  • Probiotics: an update
    Yvan Vandenplas, Geert Huys, Georges Daube
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  • Probiotics: an update
    Yvan Vandenplas, Geert Huys, Georges Daube
    Jornal de Pediatria.2015; 91(1): 6.     CrossRef
  • Probiotics and Prebiotics in Infants and Children
    Y. Vandenplas, E. De Greef, T. Devreker, G. Veereman-Wauters, B. Hauser
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The Production and Immunogenicity of Human Papillomavirus Type 58 Virus-like Particles Produced in Saccharomyces cerevisiae
Hye-Lim Kwag , Hyoung Jin Kim , Don Yong Chang , Hong-Jin Kim
J. Microbiol. 2012;50(5):813-820.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2292-1
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AbstractAbstract
Human papillomavirus (HPV) is the cause of most cases of cervical cancer. HPV type 58 (HPV58) is the second most frequent cause of cervical cancer and high-grade squamous intraepithelial lesions (HSIL) in Asia and South / Central America, respectively. However, there is no vaccine against HPV58, although there are commercially available vaccines against HPV16 and 18. In this study, we produced HPV58 L1 protein from Saccharomyces cerevisiae, and investigated its immunogenicity. We first determined the optimum period of culture for obtaining HPV58 L1. We found that a considerable portion of the HPV58 L1 resulting from 48 h culture cannot be recovered by purification, while the HPV58 L1 resulting from 144 h culture is recovered efficiently: the yield of HPV58 L1 finally recovered from 144 h culture was 2.3 times higher than that from 48 h culture, although the production level of L1 protein from 144 h culture was lower than that from 48 h culture. These results indicate that the proportion of functional L1 protein from 144 h-cultured cells is significantly higher than that of 48 h-cultured cells. The HPV58 L1 purified from the 144 h culture was correctly assembled into structures similar to naturally occurring HPV virions. Immunization with the HPV58 L1 efficiently elicited anti-HPV58 neutralizing antibodies and antigen-specific CD4+ and CD8+ T cell proliferations, without the need for adjuvant. Our findings provide a convenient method for obtaining substantial amounts of highly immunogenic HPV58 L1 from S. cerevisiae.

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Establishment and Characterization of the Epithelioma Papulosum Cyprini (EPC) Cell Line Persistently Infected with Infectious Pancreatic Necrosis Virus (IPNV), an Aquabirnavirus
Hyoung Jun Kim , Jae-Kwon Cho , Hyung-Kyu Hwang , Myung-Joo Oh , Toyohiko Nishizawa
J. Microbiol. 2012;50(5):821-826.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2364-2
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AbstractAbstract
Infectious pancreatic necrosis virus (IPNV), a type species of aquabirnaviruses in the family Birnaviridae, is an etiological agent of infectious pancreatic necrosis and has been isolated from epizootics of cultured salmonids. In the present study, an epithelioma papulosum cyprini (EPC) cell line persistently infected with IPNV (PI-EPC) was experimentally established by subculturing EPC cells surviving IPNV infection, and was characterized. PI-EPC cells were morphologically indistinguishable from EPC, but continued to grow and yield IPNV. PI-EPC cells showed no cytopathic effect due to IPNV inoculation, and susceptibility of PI-EPC cells against heterologous viruses was not different from that of EPC cells. Only one cell of 103.5 PI-EPC cells produced IPNV at approximately 100.5 50% tissue culture infectious dose (TCID50)/cell/day, which was approximately 1,000 times lower than that of normal EPC cells. PI-EPC cells that did not yield IPNV (N-PI-EPC) were screened. The IPNV genome was detected from both PI-EPC and N-PI-EPC cells, and the IPNV VP2 structural protein was detected from both cell lines, but no other IPNV proteins were observed by Western blot analysis with anti-IPNV serum. Thus, multiplication of IPNV in PI-EPC cells was regulated by some host cell factors, except interferon.

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Journal Article
Possible Translocation of Periodontal Pathogens into the Lymph Nodes Draining the Oral Cavity
G. Amodini Rajakaruna , Makoto Umeda , Keisuke Uchida , Asuka Furukawa , Bae Yuan , Yoshimi Suzuki , Ebe Noriko , Yuichi Izumi , Yoshinobu Eishi
J. Microbiol. 2012;50(5):827-836.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2030-8
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AbstractAbstract
Numerous publications have reported the presence of periodontopathogenic bacteria in peripheral and central vascular lesions. However, it is unclear how this bacterial translocation occurs. The objective of this study was to investigate whether periodontopathic bacteria are translocated to lymph nodes proximal to the oral cavity. Obtaining lymph node samples is not ethically feasible unless they are excised as part of the surgical management of patients with cancer. This study analyzed formalin-fixed and paraffin-embedded lymph nodes, histologically negative for cancer cell invasion, that were excised from 66 patients with histories of head and neck cancer. Real-time PCR was performed to amplify the 16S ribosomal DNA fragments from Porphyromonas gingivalis, Treponema denticola, Aggregatibacter actinomycetemcomitans, Tannerella forsythia, and Prevotella intermedia. The relationship between bacterial detection and cancer severity, gender, and the use of anti-cancer therapy was examined by Fisher’s exact test. P. gingivalis, T. forsythia, and P. intermedia were present in 17%, 8%, and 8% of the samples of submandibular and submental lymph nodes, respectively. There were no significant relationships between bacterial detection and the cancer disease status, patient gender or use of anticancer therapy. According to these data, it appears that the translocation of periodontopathic bacteria may occur via lymphatic drainage, irrespective of the cancer disease status, gender or anticancer therapy.

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Research Support, Non-U.S. Gov'ts
Intestinal Intraepithelial TCRγδ+ T Cells are Activated by Normal Commensal Bacteria
Sang Phil Jeong , Jung-Ah Kang , Sung-Gyoo Park
J. Microbiol. 2012;50(5):837-841.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2468-8
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AbstractAbstract
TCRγδ+ T cells play a critical role in protecting the intestinal mucosa against pathogenic infection. In the absence of infection, TCRγδ+ T cell activation must be continuously regulated by T regulatory cells (Treg) to prevent the development of colitis. However, the activation of intestinal TCRγδ+ T cells under normal conditions has not been clearly resolved. In order to determine TCRγδ+ T cell activation in vivo, we designed an NF-κB based reporter system. Using the recombinant lentiviral method, we delivered the NF-κB reporter to isolated TCRγδ+ T cells, which were then adoptively transferred into normal mice. Our data indicate that the NF-κB activation level in TCRγδ+ T cells is higher in the intestinal intraepithelial layer than in the lamina propria region. In addition, the surface expression level of lymphocyte activation marker CD69 in TCRγδ+ T cells is also higher in the intestinal intraepithelial layer and this activation was reduced by Sulfatrim treatment which removes of commensal bacteria. Collectively, our data indicate that the TCRγδ+ T cell population attached to the intestinal lumen is constitutively activated even by normal commensal bacteria.

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    Laura M. Cox, Bruna K. Tatematsu, Lydia Guo, Danielle S. LeServe, Julia Mayrink, Marilia G. Oliveira, Dustin Donnelly, Roberta C. Fonseca, Luisa Lemos, Toby B. Lanser, Ana C. Rosa, Juliana R. Lopes, Luke A. Schwerdtfeger, Gabriela F.C. Ribeiro, Eduardo L.
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Antifungal Activity of Leuconostoc citreum and Weissella confusa in Rice Cakes
Eunjong Baek , Hyojin Kim , Hyejung Choi , Sun Yoon , Jeongho Kim
J. Microbiol. 2012;50(5):842-848.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2153-y
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AbstractAbstract
The antifungal activity of organic acids greatly improves the shelf life of bread and bakery products. However, little is known about the effect of lactic acid fermentation on fungal contamination in rice cakes. Here, we show that lactic acid fermentation in rice dough can greatly retard the growth of three fungal species when present in rice cakes, namely Cladosporium sp. YS1, Neurospora sp. YS3, and Penicillium crustosum YS2. The antifungal activity of the lactic acid bacteria against these fungi was much better than that of 0.3% calcium propionate. We found that organic acids including lactic and acetic acid, which are byproducts of lactic fermentation or can be artificially added, were the main antifungal substances. We also found that some Leuconostoc citreum and Weissella confusa strains could be good starter species for rice dough fermentation. These results imply that these lactic acid bacteria can be applicable to improve the preservation of rice cakes.

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    Milind H. Gajbhiye, Balu P. Kapadnis
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    Fabricio L Tulini, Nolwenn Hymery, Thomas Haertlé, Gwenaelle Le Blay, Elaine C P De Martinis
    Journal of Dairy Research.2016; 83(1): 115.     CrossRef
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    Rui Ying Zhang, Dan Dan Hu, Yong Yue Zhang, Paul H. Goodwin, Chen Yang Huang, Qiang Chen, Wei Gao, Xiang Li Wu, Ya Jie Zou, Ji Bin Qu, Jin Xia Zhang
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    Seol Hwa Lee, Hae Choon Chang
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    Sarah Crowley, Jennifer Mahony, Douwe van Sinderen
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    Sarah Crowley, Jennifer Mahony, John P. Morrissey, Douwe van Sinderen
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NOTE] Antifungal Activity of Extracellular Hydrolases Produced by Autolysing Aspergillus nidulans Cultures
Melinda Szilágyi , Fruzsina Anton , Katalin Forgács , Jae-Hyuk Yu , István Pócsi , Tamás Emri
J. Microbiol. 2012;50(5):849-854.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2001-0
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AbstractAbstract
Carbon-starving Aspergillus nidulans cultures produce high activities of versatile hydrolytic enzymes and, among these, ChiB endochitinase and EngA β-1,3-endoglucanase showed significant antifungal activity against various fungal species. Double deletion of engA and chiB diminished the antifungal activity of the fermentation broths and increased conidiogenesis and long-term viability of A. nidulans, but decreased the growth rate on culture media containing weak carbon sources. Production of ChiB and EngA can influence fungal communities either directly due to their antifungal properties or indirectly through their effects on vegetative growth. Our data suggest saprophytic fungi as promising future candidates to develop novel biocontrol technologies.

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    Melinda Szilágyi, Fruzsina Anton, István Pócsi, Tamás Emri
    Journal of Basic Microbiology.2018; 58(5): 440.     CrossRef
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    Soumya Mukherjee, Mathu Malar Chandrababunaidu, Arijit Panda, Suman Khowala, Sucheta Tripathy
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    Zsolt Spitzmüller, Nak-Jung Kwon, Melinda Szilágyi, Judit Keserű, Viktória Tóth, Jae-Hyuk Yu, István Pócsi, Tamás Emri
    Archives of Microbiology.2015; 197(2): 285.     CrossRef
  • Investigating Aspergillus nidulans secretome during colonisation of cork cell walls
    Isabel Martins, Helga Garcia, Adélia Varela, Oscar Núñez, Sébastien Planchon, Maria Teresa Galceran, Jenny Renaut, Luís P.N. Rebelo, Cristina Silva Pereira
    Journal of Proteomics.2014; 98: 175.     CrossRef
  • Transcriptome changes initiated by carbon starvation in Aspergillus nidulans
    Melinda Szilágyi, Márton Miskei, Zsolt Karányi, Béla Lenkey, István Pócsi, Tamás Emri
    Microbiology.2013; 159(Pt_1): 176.     CrossRef
  • Interactions between naturally occurring antifungal agents
    Viktória Tóth, Melinda Szilágyi, Fruzsina Anton, Éva Leiter, I. Pócsi, T. Emri
    Acta Biologica Hungarica.2013; 64(4): 510.     CrossRef
NOTE] Burkholderia denitrificans sp. nov., Isolated from the Soil of Dokdo Island, Korea
Chang-Muk Lee Lee , Hang-Yeon Weon , Sang-Hong Yoon , Soo-Jin Kim , Bon-Sung Koo , Soon-Wo Kwon
J. Microbiol. 2012;50(5):855-859.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-1554-2
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AbstractAbstract
A novel, Gram-negative, bacterial strain KIS30-44T was identified from wet forest soil collected on the Korean island of Dokdo. Growth of the strain was observed at 15–30°C, pH 5−9, 0–3% NaCl, and 950 mM KNO3. KIS30-44T reduced nitrate to nitrogen gas. Analysis of the 16S rRNA gene sequence showed that KIS30-44T was phylogenetically related to Burkholderia sacchari, Burkholderia mimosarum, and Burkholderia oxyphila (98.1%, 98.0%, and 98.0% sequence similarity, respectively). The genomic G+C content was 63.5 mol%. KIS30-44T exhibited less than 52% DNA-DNA relatedness with the type strains of 9 closely related Burkholderia species. The major isoprenoid quinone was Q-8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unknown aminolipids. The major fatty acids in KIS30-44T were C16:0, C18:1 ω7c and summed feature 3 (iso-C15:0 2-OH and C16:1 ω7c), and the strain contained half the amount of C17:0 cyclo found in the 9 closely related Burkholderia species. The results of these phenotypic, 16S rRNA gene sequence, DNA-DNA hybridization, and chemotaxonomic data indicate that KIS30-44T represents a novel species within the genus Burkholderia, for which the name Burkholderia denitrificans (Type strain KIS30-44T =KACC 12733T =DSM 24336T) is proposed.

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    Chrizelle W. Beukes, Stephanus N. Venter, Emma T. Steenkamp
    Grass and Forage Science.2021; 76(1): 10.     CrossRef
  • Paraburkholderia madseniana sp. nov., a phenolic acid-degrading bacterium isolated from acidic forest soil
    Roland C. Wilhelm, Sean J. L. Murphy, Nicole M. Feriancek, David C. Karasz, Christopher M. DeRito, Jeffrey D. Newman, Daniel H. Buckley
    International Journal of Systematic and Evolutionary Microbiology .2020; 70(3): 2137.     CrossRef
  • Paraburkholderia lycopersici sp. nov., a nitrogen-fixing species isolated from rhizoplane of Lycopersicon esculentum Mill. var. Saladette in Mexico
    Erika Yanet Tapia-García, Ivan Arroyo-Herrera, Fernando Uriel Rojas-Rojas, J. Antonio Ibarra, María Soledad Vásquez-Murrieta, Lourdes Martínez-Aguilar, Isabel M. López-Lara, William B. Whitman, Paulina Estrada de los Santos
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    Zhe Kong, Lu Li, Jiang Wu, Tao Zhang, Yu-You Li
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  • Paraburkholderia dokdonella sp. nov., isolated from a plant from the genus Campanula
    Man-Young Jung, Myung-Suk Kang, Ki-Eun Lee, Eun-Young Lee, Soo-Je Park
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    Seil Kim, Gyeongtaek Gong, Han Min Woo, Yunje Kim, Youngsoon Um
    International Journal of Systematic and Evolutionary Microbiology.2016; 66(3): 1260.     CrossRef
  • Burkholderia humisilvae sp. nov., Burkholderia solisilvae sp. nov. and Burkholderia rhizosphaerae sp. nov., isolated from forest soil and rhizosphere soil
    Jae-Chan Lee, Kyung-Sook Whang
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  • Molecular signatures and phylogenomic analysis of the genus Burkholderia: proposal for division of this genus into the emended genus Burkholderia containing pathogenic organisms and a new genus Paraburkholderia gen. nov. harboring environmental species
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  • List of new names and new combinations previously effectively, but not validly, published

    International Journal of Systematic and Evolutionary Microbiology .2013; 63(Pt_1): 1.     CrossRef
NOTE] Next-Generation Sequencing-Based Genome-Wide Mutation Analysis of L-Lysine-Producing Corynebacterium glutamicum ATCC 21300 Strain
Chang-Soo Lee , Jae-Young Nam , Eun-Suk Son , O-chul Kwon , Woorijarang Han , Jae-Yong Cho , Young-Jin Park
J. Microbiol. 2012;50(5):860-863.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2109-2
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AbstractAbstract
In order to identify single nucleotide polymorphism and insertion/deletion mutations, we performed whole-genome re-sequencing of the enhanced L-lysine-producing Corynebacterium glutamicum ATCC 21300 strain. In total, 142 single nucleotide polymorphisms and 477 insertion/deletion mutations were identified in the ATCC 21300 strain when compared to 3,434 predicted genes of the wild-type C. glutamicum ATCC 13032 strain. Among them, 110 transitions and 29 transversions of single nucleotide polymorphisms were found from genes of the ATCC 21300 strain. In addition, 11 genes, involved in the L-lysine biosynthetic pathway and central carbohydrate metabolism, contained mutations including single nucleotide polymorphisms and insertions/deletions. Interestingly, RT-PCR analysis of these 11 genes indicated that they were normally expressed in the ATCC 21300 strain. This information of genome-wide gene-associated variations will be useful for genome breeding of C. glutamicum in order to develop an industrial amino acidproducing strain with minimal mutation.

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  • Review of the Proteomics and Metabolic Properties of Corynebacterium glutamicum
    Juhwan Park, Sooa Lim
    Microorganisms.2024; 12(8): 1681.     CrossRef
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    Yusheng Wang, Yunlong Bai, Qi Zeng, Zeyuan Jiang, Yuzhe Liu, Xiyan Wang, Xiaoting Liu, Chunlei Liu, Weihong Min
    International Journal of Biological Macromolecules.2023; 253: 126916.     CrossRef
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    Ning Li, Weizhu Zeng, Sha Xu, Jingwen Zhou
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    Gabriela Bukovska, Jana Ugorcakova, Nora Halgasova, Lucia Bocanova, Adela Tkacova
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    Fernanda Karine do Carmo Félix, Luiz Alberto Junior Letti, Gilberto Vinícius de Melo Pereira, Pedro Gabriel Borges Bonfim, Vanete Thomaz Soccol, Carlos Ricardo Soccol
    Critical Reviews in Biotechnology.2019; 39(8): 1031.     CrossRef
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    Junjie Yang, Sheng Yang
    BMC Genomics.2017;[Epub]     CrossRef
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    S. Heux, I. Meynial-Salles, M.J. O'Donohue, C. Dumon
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    Makoto Uchida, Takashi Hirasawa, Masaaki Wachi
    Archives of Microbiology.2014; 196(12): 871.     CrossRef
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    Hong-Il Kim, Jae-Young Nam, Jae-Yong Cho, Chang-Soo Lee, Young-Jin Park
    Journal of Microbiology.2013; 51(6): 877.     CrossRef
NOTE] Caenimonas terrae sp. nov., Isolated from a Soil Sample in Korea, and Emended Description of the Genus Caenimonas Ryu et al. 2008
Soo-Jin Kim , Hang-Yeon Weon , Yi-Seul Kim , Ji Young Moon , Soon Ja Seok , Seung-Beom Hong , Soon-Wo Kwon
J. Microbiol. 2012;50(5):864-868.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-1587-6
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AbstractAbstract
A white-coloured bacterium, SGM1-15T, was isolated from a paddy soil sample from Suwon, Republic of Korea. The cells were strictly aerobic, Gram-negative and curved rodshaped. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SGM1-15T was closely related to Curvibacter delicatus LMG 4328T (97.6% similarity) and Caenimonas koreensis EMB320T (97.5% similarity). The major respiratory quinone system was Q-8 and the predominant cellular fatty acids were C16:0 (39.9%), summed feature 3 (C16:1 ω7c and/or iso-C15:0 2-OH; 24.3%) and C17:0 cyclo (22.7%). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major polyamines were 2-hydroxypurescine, purescine and spermidine. The DNA G+C content was 68.7 mol%. On the basis of the phylogenetic, physiologicl and chemotaxonomic data, stain SGM1-15T represents a novel species of the genus Caenimonas, for which the name Caenimonas terrae sp. nov. is proposed. The type strain of Caenimonas terrae is SGM1-15T (=KACC 13365T =NBRC 106341T).
NOTE] Envelope Diversity, Characteristics of V3 Region and Predicted Co-Receptor Usage of Human Immunodeficiency Viruses Infecting North Indians
Raiees Andrabi , Rajesh Kumar , Manju Bala , Ambili Nair , Prakash SS , Vandana Kushwaha , Kalpana Luthra
J. Microbiol. 2012;50(5):869-873.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2136-z
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AbstractAbstract
Subtypes of human immunodeficiency virus type 1 circulating in 21 north Indian patients were characterized based on the partial sequence of the gp120 envelope protein. A majority of viruses (85.7%, 18/21) were subtype C, while 14.3% (3/21) were subtype A. Sequence analysis revealed that the V3 region was highly conserved compared with V4 and V5. The predicted use of co-receptors indicated exclusive usage of R5, except for two subtype A viruses (AIIMS279 and AIIMS281). Our results demonstrate conservation within the V3 loop of subtype C viruses, and suggest the emergence of non-clade C viruses in the north Indian population.
NOTE] Construction and Characterisation of an Antifungal Recombinant Bacillus thuringiensis with an Expanded Host Spectrum
Qin Liu , Jong Yul Roh , Yong Wang , Jae Young Choi , Xue Ying Tao , Jae Su Kim , Yeon Ho Je
J. Microbiol. 2012;50(5):874-877.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2201-7
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AbstractAbstract
A novel antifungal Bacillus thuringiensis strain 19–22, ssp. kurstaki (H3a3b3c), was characterised. This strain included cry1Aa, cry1Ab, cry1Ac, and cry1D, which have high insecticidal activities against lepidopteran larvae other than Spodoptera exigua. To expand the host spectrum, a cry1E gene whose product is active against S. exigua was introduced into the isolate. The transformant successfully expressed the Cry1E protein without any loss of its original antifungal activities. These results indicate that this recombinant strain exhibits dual activities and may be used as an integrated control agent to control plant diseases and insect pests.
NOTE] Regulatory Characteristics of the Vibrio vulnificus rtxHCA Operon Encoding a MARTX Toxin
Jinseo Park , Seung Min Kim , Hee Gon Jeong , Sang Ho Choi
J. Microbiol. 2012;50(5):878-881.   Published online September 21, 2012
DOI: https://doi.org/10.1007/s12275-012-2479-5
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AbstractAbstract
Vibrio vulnificus MARTX encoded by rtxA, an open reading frame of the rtxHCA operon, is essential for virulence in vitro and in mice. In this study, a primer extension analysis revealed that transcription of the rtxHCA operon begins at a single site, and is under the direction of a single promoter, PrtxHCA. PrtxHCA activity appeared at the beginning of growth and reached a maximum in mid-exponential phase. PrtxHCA activity was induced by exposure to INT-407 cells, and the membrane fraction of INT-407 cells was the most effective for the induction.

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  • Rifampicin‐resistant RpoB S522L Vibrio vulnificus exhibits disturbed stress response and hypervirulence traits
    Laura Cutugno, Conor O'Byrne, Jan Pané‐Farré, Aoife Boyd
    MicrobiologyOpen.2023;[Epub]     CrossRef
  • Vibrio vulnificus PlpA facilitates necrotic host cell death induced by the pore forming MARTX toxin
    Changyi Cho, Sanghyeon Choi, Myung Hee Kim, Byoung Sik Kim
    Journal of Microbiology.2022; 60(2): 224.     CrossRef
  • A MARTX Toxin rtxA Gene Is Controlled by Host Environmental Signals through a CRP-Coordinated Regulatory Network in Vibrio vulnificus
    Zee-Won Lee, Seung-Ho Hwang, Garam Choi, Kyung Ku Jang, Tae Hee Lee, Kyung Min Chung, Byoung Sik Kim, Sang Ho Choi, Caroline S. Harwood
    mBio.2020;[Epub]     CrossRef
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    Byoung Sik Kim
    Toxins.2020; 12(9): 544.     CrossRef
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    Jong Gyu Lim, Jin Hwan Park, Sang Ho Choi
    Journal of Microbiology.2014; 52(5): 413.     CrossRef
  • VvpM, an extracellular metalloprotease of Vibrio vulnificus, induces apoptotic death of human cells
    Mi-Ae Lee, Jeong-A Kim, Yu Jin Yang, Mee-Young Shin, Soon-Jung Park, Kyu-Ho Lee
    Journal of Microbiology.2014; 52(12): 1036.     CrossRef
NOTE] Identification of Chaperones in Freeze Tolerance in Saccharomyces cerevisiae
Mahendran Chinnamara Naicker , I Seul Jo , Hana Im
J. Microbiol. 2012;50(5):882-887.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2411-z
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AbstractAbstract
Exposure to low temperatures reduces protein folding rates and induces the cold denaturation of proteins. Considering the roles played by chaperones in facilitating protein folding and preventing protein aggregation, chaperones must exist that confer tolerance to cold stress. Here, yeast strains lacking individual chaperones were screened for reduced freezing tolerance. In total, 19 of 82 chaperone-deleted strains tested were more sensitive to freeze-thaw treatment than wild-type cells. The reintroduction of the respective chaperone genes into the deletion mutants recovered the freeze tolerance. The freeze sensitivity of the chaperone-knockout strains was also retained in the presence of 20% glycerol.
Journal Article
NOTE] Winogradskyella jejuensis sp. nov., a Marine Bacterium Isolated from a Brown Alga Carpopeltis affinis
Ji-Young Kim , Duck-Chul Oh
J. Microbiol. 2012;50(5):888-892.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2447-0
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AbstractAbstract
A Gram-negative, orange-pigmented, rod-shaped bacterium, designated strain CP32T was isolated from a brown alga Carpopeltis affinis collected on the coast of Jeju Island, Republic of Korea. The isolate grew at 10–37°C (optimum 25°C) and at pH 6.5–9.5 (optimum pH 7.0). The 16S rRNA gene sequence of the isolate showed much similarity with the type strains of recognized species of the genus Winogradskyella (94.0–96.6%). The most closely related species were Winogradskyella echinorum KMM 6211T, Winogradskyella ulvae KMM 6390T, Winogradskyella thalassocola KMM 3907T, Winogradskyella poriferorum UST030701-295T, and Winogradskyella eximia KMM 3944T. The major respiratory quinone was menaquinone-6 (MK-6) and the predominant cellular fatty acids were iso-C15:1 G (24.8%), iso-C15:0 (23.4%), and iso-C17:0 3-OH (11.6 %). The DNA G+C content was 33.3 mol%. The polar lipid profile was composed of phosphatidylethanolamine, two aminolipids, and five unknown lipids. On the basis of phenotypic features, and the result of 16S rRNA gene sequence analysis, strain CP32T (=KCTC 23835T =JCM 18454T) represents a novel species of the genus Winogradskyella, for which the name Winogradskyella jejuensis sp. nov. is proposed.
Erratum] Phycicoccus ochangensis sp. nov., Isolated from Soil of a Potato Cultivation Field
Hyangmi Kim , Hyun-Woo Oh , Doo-Sang Park , Kang Hyun Lee , Sung Uk Kim , Hee-Moon Park , Kyung Sook Bae Bae
J. Microbiol. 2012;50(5):893-893.
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AbstractAbstract
In the article by Kim et al. that appears in the Journal of Microbiology 2012; 50, 349-353. KCTC number on page 349 (abstract line 19) and page 353 (left paragraph line 32) should read as KCTC 19695 not KCTC 19694, and on page 353 (left paragraph line 33) should read as KCTC 19694 not KCTC 19695.

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