Journal of Microbiology

Yong Heon Lee

2 Articles

Impact of small RNA RaoN on nitrosative-oxidative stress resistance and virulence of Salmonella enterica serovar Typhimurium: Sinyeon Kim , Yong Heon Lee; J. Microbiol. 2020;58(6):499-506. Published online April 11, 2020; DOI: https://doi.org/10.1007/s12275-020-0027-2

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RaoN is a Salmonella-specific small RNA that is encoded in the cspH-envE intergenic region on Salmonella pathogenicity island-11. We previously reported that RaoN is induced under conditions of acid and oxidative stress combined with nutrient limitation, contributing to the intramacrophage growth of Salmonella enterica serovar Typhimurium. However, the role of RaoN in nitrosative stress response and virulence has not yet been elucidated. Here we show that the raoN mutant strain has increased susceptibility to nitrosative stress by using a nitric oxide generating acidified nitrite. Extending previous research on the role of RaoN in oxidative stress resistance, we found that NADPH oxidase inhibition restores the growth of the raoN mutant in LPS-treated J774A.1 macrophages. Flow cytometry analysis further revealed that the inactivation of raoN leads to an increase in the intracellular level of reactive oxygen species (ROS) in Salmonella-infected macrophages, suggesting that RaoN is involved in the inhibition of NADPH oxidase-mediated ROS production by mechanisms not yet resolved. Moreover, we evaluated the effect of raoN mutation on the virulence in murine systemic infection and determined that the raoN mutant is less virulent than the wild-type strain following oral inoculation. In
conclusion
, small regulatory RNA RaoN controls nitrosativeoxidative stress resistance and is required for virulence of Salmonella in mice.

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The Salmonella enterica EnvE is an Outer Membrane Lipoprotein and Its Gene Expression Leads to Transcriptional Repression of the Virulence Gene msgA
Sinyeon Kim, Yong Heon Lee
Journal of Microbiology.2024; 62(11): 1013. CrossRef
Functions of Small Non-Coding RNAs in Salmonella–Host Interactions
Xia Meng, Mengping He, Pengpeng Xia, Jinqiu Wang, Heng Wang, Guoqiang Zhu
Biology.2022; 11(9): 1283. CrossRef
Detoxification Response of Pseudomonas fluorescens MFAF76a to Gaseous Pollutants NO2 and NO
Thibault Chautrand, Ségolène Depayras, Djouhar Souak, Mathilde Bouteiller, Tatiana Kondakova, Magalie Barreau, Mohamed Amine Ben Mlouka, Julie Hardouin, Yoan Konto-Ghiorghi, Sylvie Chevalier, Annabelle Merieau, Nicole Orange, Cécile Duclairoir-Poc
Microorganisms.2022; 10(8): 1576. CrossRef
Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Microbial Pathogenesis.2022; 165: 105460. CrossRef
Gaseous NO2 induces various envelope alterations in Pseudomonas fluorescens MFAF76a
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Scientific Reports.2022;[Epub] CrossRef
Current challenges facing one-step production of l-ascorbic acid
Panpan Wang, Weizhu Zeng, Sha Xu, Guocheng Du, Jingwen Zhou, Jian Chen
Biotechnology Advances.2018; 36(7): 1882. CrossRef

Direct interaction between the transcription factors CadC and OmpR involved in the acid stress response of Salmonella enterica: Yong Heon Lee , Ji Hye Kim; J. Microbiol. 2017;55(12):966-972. Published online December 7, 2017; DOI: https://doi.org/10.1007/s12275-017-7410-7

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Abstract

In Salmonella enterica serovar Typhimurium, the acid-sensing regulator CadC activates transcription of the cadBA operon which contributes to the acid tolerance response. The DNA-binding response regulator OmpR in two-component regulatory system with EnvZ binds to its own promoter for autoinduction. We previously reported that CadC exerts a negative influence on ompR transcription during acid adaptation. However, its underlying mechanisms remain to be elucidated. Here we show that the level of OmpR protein is gradually reduced by a gradual increase in the CadC level using an arabinose-inducible expression system, indicating there exists a negative correlation between the expression levels of two transcription factors. To explore the molecular basis for OmpR repression by CadC, we performed in vitro binding assays and determined that CadC directly interacts with OmpR. We further show that inactivation of cadC inhibits transcription of the fliC gene, which encodes the major flagellar subunit,
result
ing in impaired flagellar motility under acid-adaptation conditions. Together, our findings suggest that CadC may repress autoinduction of the OmpR response regulator through their direct interaction.

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Isolation and characterization of resistant variants of Salmonella Typhimurium after sequential exposure to plasma activated water (PAW)
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Innovative Food Science & Emerging Technologies.2024; 93: 103633. CrossRef
A current insight into Salmonella’s inducible acid resistance
Alkmini Gavriil, Ilias Giannenas, Panagiotis N. Skandamis
Critical Reviews in Food Science and Nutrition.2024; : 1. CrossRef
Molecular mechanism of proteolytic cleavage-dependent activation of CadC-mediated response to acid in E. coli
Min Chen, Ye Shang, Wenhao Cui, Xiaomeng Wang, Jiakun Zhu, Hongjie Dong, Hongwei Wang, Tiantian Su, Weiwei Wang, Kundi Zhang, Bingqing Li, Sujuan Xu, Wei Hu, Fengyu Zhang, Lichuan Gu
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Transmembrane Transcription Regulators Are Widespread in Bacteria and Archaea
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Microbiology Spectrum.2023;[Epub] CrossRef
Host acid signal controls Salmonella flagella biogenesis through CadC-YdiV axis
Weiwei Wang, Yingying Yue, Min Zhang, Nannan Song, Haihong Jia, Yuanji Dai, Fengyu Zhang, Cuiling Li, Bingqing Li
Gut Microbes.2022;[Epub] CrossRef
Recent advances in understanding the effect of acid-adaptation on the cross-protection to food-related stress of common foodborne pathogens
Ricardo A. Wu, Hyun-Gyun Yuk, Donghong Liu, Tian Ding
Critical Reviews in Food Science and Nutrition.2022; 62(26): 7336. CrossRef
Metabolic adaptability shifts of cell membrane fatty acids of Komagataeibacter hansenii HDM1-3 improve acid stress resistance and survival in acidic environments
Yuanjing Li, Pengfei Yan, Qingyun Lei, Bingyu Li, Yue Sun, Shuangfei Li, Hong Lei, Ning Xie
Journal of Industrial Microbiology and Biotechnology.2019; 46(11): 1491. CrossRef

Yong Heon Lee

1 Article

The Salmonella enterica EnvE is an Outer Membrane Lipoprotein and Its Gene Expression Leads to Transcriptional Repression of the Virulence Gene msgA: Sinyeon Kim, Yong Heon Lee; J. Microbiol. 2024;62(11):1013-1022. Published online November 15, 2024; DOI: https://doi.org/10.1007/s12275-024-00183-4

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Abstract: The envE gene of Salmonella enterica serovar Typhimurium is encoded within Salmonella Pathogenicity Island-11 (SPI-11) and is located immediately upstream of the virulence gene msgA (macrophage survival gene A) in the same transcriptional orientation. To date, the characteristics and roles of envE remain largely unexplored. In this study, we show that EnvE, a predicted lipoprotein, is localized on the outer membrane using sucrose gradient ultracentrifugation. Under oxidative stress conditions, envE transcription is suppressed, while msgA transcription is induced, indicating an inverse correlation between the mRNA levels of the two neighboring genes. Importantly, inactivation of envE leads to constitutive transcription of msgA regardless of the presence of oxidative stress. Moreover, trans-complementation of the envE mutant with a plasmid-borne envE fails to prevent the induction of msgA transcription, suggesting that envE functions as a cis-regulatory element rather than a trans-acting factor. We further show that both inactivation and complementation of envE confer wild-type levels of resistance to oxidative stress by ensuring the expression of msgA. Our data suggest that the S. enterica envE gene encodes an outer membrane lipoprotein, and its transcription represses msgA expression in a cis-acting manner, probably by transcriptional interference, although the exact molecular details are yet unclear.

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