- Flaviflexus equikiangi sp. nov. isolated from faeces of Equus kiang (Tibetan wild ass) and carrying a class 1 integron gene cassette in its genome
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Caixin Yang , Xingxing Lian , Yanpeng Cheng , Yifan Jiao , Jing Yang , Kui Dong , Shan Lu , Xin-He Lai , Dong Jin , Han Zheng , Ji Pu , Suping Wang , Liyun Liu , Jianguo Xu
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J. Microbiol. 2022;60(6):585-593. Published online April 18, 2022
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DOI: https://doi.org/10.1007/s12275-022-1673-3
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 Abstract
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Two Gram-stain-positive, catalase-negative, non-spore-forming,
cocci-shaped strains (dk850T and JY899) were isolated
from the feces of Equus kiang in the Qinghai-Tibet Plateau of
China. 16S rRNA gene sequence-based phylogenetic analyses
showed that strains dk850T and JY899 belong to the genus
Flaviflexus, closest to F. salsibiostraticola KCTC 33148T, F. ciconiae
KCTC 49253T and F. huanghaiensis H5T. The DNA
G + C content of strain dk850T was 62.9%. The digital DNADNA
hybridization values of strain dk850T with the closely related
species were below the 70% threshold for species demarcation.
The two strains grew best at 28°C on brain heart infusion
(BHI) agar with 5% sheep blood. All strains had C18:1ω9c
and C16:0 as the major cellular fatty acids. MK-9(H4) was the
major menaquinone in strain dk850T. The major polar lipids
included diphosphatidylglycerol and an unidentified phospholipid.
Strains dk850T and JY899 were identified as carrying
a class 1 integron containing the aminoglycoside resistance
gene aadA11, both strains were resistant to spectinomycin
and streptomycin. Based on several lines of evidence
from phenotypic and phylogenetic analyses, strains dk850T
and JY899 represent a novel species of the genus Flaviflexus,
for which the name Flaviflexus equikiangi sp. nov. is proposed.
The type strain is dk850T (= CGMCC 1.16593T = JCM 33598T).
- Changpingibacter yushuensis gen. nov., sp. nov., isolated from fluvial sediment in Qinghai Tibet Plateau of China
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Yifan Jiao , Sihui Zhang , Jing Yang , Xin-He Lai , Kui Dong , Yanpeng Cheng , Mingchao Xu , Wentao Zhu , Shan Lu , Dong Jin , Ji Pu , Ying Huang , Liyun Liu , Suping Wang , Jianguo Xu
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J. Microbiol. 2022;60(2):147-155. Published online January 7, 2022
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DOI: https://doi.org/10.1007/s12275-022-1199-8
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3
Web of Science
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Abstract
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Two facultatively anaerobic, short rod-shaped, non-motile,
Gram-stain-positive, unknown bacterial strains (JY-X040T
and JY-X174) were isolated from fluvial sediments of Tongtian
River in Yushu Tibetan Autonomous Prefecture, Qinghai
province, China. Cells formed translucent, gray, round and
convex colonies, with a diameter of less than 0.5 mm after 5
days of incubation at 30°C on brain heart infusion-5% sheep
blood agar. The 16S rRNA gene sequence similarity between
strain JY-X040T and Fudania jinshanensis 313T is 93.87%.
In the four phylogenetic trees constructed based on the 16S
rRNA gene and 423 core genes, the two isolates form an independent
branch, phylogenetically closest to F. jinshanensis
313T, but could not be classified as a member of the genus
Fudania or any other genus of the family Arcanobacteriaceae.
The DNA G + C content of strain JY-X040T was 57.8%. Calculation
results
of average nucleotide identity, digital DNADNA
hybridization value and amino acid identity between
strain JY-X040T and F. jinshanensis 313T are 69.9%, 22.9%,
and 64.1%. The major cellular fatty acids were C16:0 (23%)
and C18:1ω9c (22%). The cell-wall peptidoglycan type was A5α
(L-Lys-L-Ala-L-Lys-D-Glu). The polar lipids comprised diphosphatidylglycerol,
phosphatidylglycerol, phosphatidylinositol,
phosphatidylinositol mannoside and four unidentified components.
The whole-cell sugars contained rhamnose and ribose.
MK-10(H4) was the sole respiratory quinone. The minimum
inhibitory concentration of streptomycin was 32 μg/ml. All
physiological, biochemical, chemotaxonomic and genomic
characteristics support that strains JY-X040T and JY-X174
represent members of a novel species in a new genus, Changpingibacter
yushuensis gen. nov., sp. nov. The type strain is
JY-X040T (GDMCC 1.1996T = KCTC 49514T).
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Citations
Citations to this article as recorded by  - Metagenomic and Culturomics Analysis of Microbial Communities within Surface Sediments and the Prevalence of Antibiotic Resistance Genes in a Pristine River: The Zaqu River in the Lancang River Source Region, China
Yi Yan, Jialiang Xu, Wenmin Huang, Yufeng Fan, Zhenpeng Li, Mingkai Tian, Jinsheng Ma, Xin Lu, Jian Liang
Microorganisms.2024; 12(5): 911. CrossRef - Bacterial diversity in arboreal ant nesting spaces is linked to colony developmental stage
Maximilian Nepel, Veronika E. Mayer, Veronica Barrajon-Santos, Dagmar Woebken
Communications Biology.2023;[Epub] CrossRef
- Agromyces laixinhei sp. nov. isolated from bat feces in China
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Yanpeng Cheng , Yibo Bai , Yuyuan Huang , Jing Yang , Shan Lu , Dong Jin , Ji Pu , Han Zheng , Junqin Li , Ying Huang , Suping Wang , Jianguo Xu
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J. Microbiol. 2021;59(5):467-475. Published online March 29, 2021
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DOI: https://doi.org/10.1007/s12275-021-0546-5
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74
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Abstract
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Three rod-shaped, Gram-stain-positive, and catalase-positive,
phenotypically closely related isolates (HY052T, HY050, and
HY045) were obtained from fecal samples collected from bats
in Guangxi province and Chongqing city of China. Circular,
smooth, light-yellow colonies appeared on brain heart infusion
plate after 24–48 h incubation at 28°C. The optimal pH
for growth was between 6.0 and 7.5. Based on 16S rRNA, the
three isolates were phylogenetically related to Agromyces terreus
DS-10T, Agromyces aureus AR33T, Agromyces salentinus
20-5T, Agromyces allii UMS-62T, Agromyces lapidis CD55T,
and Agromyces italicus CD1T. Moreover, based on 296 core
genes, the phylogenomic tree indicated that the three isolates
clustered together, closest to Agromyces cerinus VKM Ac-
1340T and Agromyces fucosus VKM Ac-1345T but separated
distantly from other Agromyces species. The average nucleotide
identity values between strain HY052T and other Agromyces
species ranged from 79.3% to 87.9%, lower than the
95–96% threshold. Furthermore, the genome of strain HY052T
contains a circular chromosome of 3,437,203 bp with G + C
content of 69.0 mol%. Main fatty acids were anteiso-C15:0
and anteiso-C17:0. The polar lipids comprised diphosphatidylglycerol,
phosphatidylglycerol, and unidentified glycolipids.
Rhamnose, ribose, and glucose were the primary cell wall sugars.
The major peptidoglycan amino acids included alanine,
glutamic acid, glycine, and 2,4-diaminobutyric acid. An additional
remarkable difference from other Agromyces species
is that MK-12 was the sole menaquinone in strain HY052T.
Based on results from the polyphasic characterizations performed
in this study, our isolates are proposed to be members
of a novel species in genus Agromyces, named Agromyces
laixinhei. The type strain is HY052T (= CGMCC 1.17175T =
JCM 33695T).
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Citations
Citations to this article as recorded by -
Agromyces archimandritae sp. nov., isolated from the cockroach Archimandrita tessellata
Juan Guzman, Maite Ortúzar, Anja Poehlein, Rolf Daniel, Martha E. Trujillo, Andreas Vilcinskas
International Journal of Systematic and Evolutionary Microbiology
.2022;[Epub] CrossRef - The Threat of Potentially Pathogenic Bacteria in the Feces of Bats
Yuyuan Huang, Yamin Sun, Qianni Huang, Xianglian Lv, Ji Pu, Wentao Zhu, Shan Lu, Dong Jin, Liyun Liu, Zhengli Shi, Jing Yang, Jianguo Xu, Biao He
Microbiology Spectrum.2022;[Epub] CrossRef - Morphological and genomic characteristics of two novel halotolerant actinomycetes, Tomitella gaofuii sp. nov. and Tomitella fengzijianii sp. nov. isolated from bat faeces
Yuyuan Huang, Jing Yang, Shan Lu, Xin-He Lai, Dong Jin, Juan Zhou, Sihui Zhang, Qianni Huang, Xianglian Lv, Wentao Zhu, Ji Pu, Ying Huang, Liyun Liu, Jianguo Xu
Systematic and Applied Microbiology.2022; 45(2): 126294. CrossRef - Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp. nov., a novel halotolerant actinomycete isolated from bat feces
Yuyuan Huang, Lingzhi Dong, Jian Gong, Jing Yang, Shan Lu, Xin-He Lai, Dong Jin, Qianni Huang, Ji Pu, Liyun Liu, Jianguo Xu
Journal of Microbiology.2022; 60(10): 977. CrossRef
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