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Woojun Park 16 Articles
The β‑Lactamase Activity at the Community Level Confers β‑Lactam Resistance to Bloom‑Forming Microcystis aeruginosa Ce
Yerim Park , Wonjae Kim , Minkyung Kim , Woojun Park
J. Microbiol. 2023;61(9):807-820.   Published online October 18, 2023
DOI: https://doi.org/10.1007/s12275-023-00082-0
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  • 9 Web of Science
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AbstractAbstract PDF
Many freshwater cyanobacteria, including Microcystis aeruginosa, lack several known antibiotic resistance genes; however, both axenic and xenic M. aeruginosa strains exhibited high antibiotic resistance against many antibiotics under our tested concentrations, including colistin, trimethoprim, and kanamycin. Interestingly, axenic PCC7806, although not the xenic NIBR18 and NIBR452 strains, displayed susceptibility to ampicillin and amoxicillin, indicating that the associated bacteria in the phycosphere could confer such antibiotic resistance to xenic strains. Fluorescence and scanning electron microscopic observations revealed their tight association, leading to possible community-level β-lactamase activity. Combinatory treatment of ampicillin with a β-lactamase inhibitor, sulbactam, abolished the ampicillin resistance in the xenic stains. The nitrocefin-based assay confirmed the presence of significant community-level β-lactamase activity. Our tested low ampicillin concentration and high β-lactamase activity could potentially balance the competitive advantage of these dominant species and provide opportunities for the less competitive species, thereby resulting in higher bacterial diversity under ampicillin treatment conditions. Non-PCR-based metagenome data from xenic NIBR18 cultures revealed the dominance of blaOXArelated antibiotic resistance genes followed by other class A β-lactamase genes (AST-1 and FAR-1). Alleviation of ampicillin toxicity could be observed only in axenic PCC7806, which had been cocultured with β-lactamase from other freshwater bacteria. Our study suggested M. aeruginosa develops resistance to old-class β-lactam antibiotics through altruism, where associated bacteria protect axenic M. aeruginosa cells.

Citations

Citations to this article as recorded by  
  • Public goods-mediated bacterial interplay in aquatic ecosystems
    Yerim Park, Wonjae Kim, Jihye Bae, Woojun Park
    Water Research.2025; 287: 124310.     CrossRef
  • Selective cyanobactericidal activity of Nocardioides convexus MS16 against Microcystis aeruginosa, mediated by direct attack
    Seonah Jeong, Ve Van Le, So-Ra Ko, Mingyeong Kang, Min-Seong Kim, Zhun Li, Chi-Yong Ahn
    Algal Research.2025; 90: 104165.     CrossRef
  • Sustainable control of Microcystis aeruginosa, a harmful cyanobacterium, using Selaginella tamariscina extracts
    Wonjae Kim, Yerim Park, Minkyung Kim, Yeji Cha, Jaejoon Jung, Che Ok Jeon, Woojun Park
    Ecotoxicology and Environmental Safety.2024; 277: 116375.     CrossRef
  • Microcystis abundance is predictable through ambient bacterial communities: A data-oriented approach
    Mingyeong Kang, Dong-Kyun Kim, Ve Van Le, So-Ra Ko, Jay Jung Lee, In-Chan Choi, Yuna Shin, Kyunghyun Kim, Chi-Yong Ahn
    Journal of Environmental Management.2024; 368: 122128.     CrossRef
  • Enhanced mechanical properties of living and regenerative building materials by filamentous Leptolyngbya boryana
    Yongjun Son, Jihyeon Min, Indong Jang, Jiyoon Park, Chongku Yi, Woojun Park
    Cell Reports Physical Science.2024; 5(8): 102098.     CrossRef
  • Food Webs and Feedbacks: The Untold Ecological Relevance of Antimicrobial Resistance as Seen in Harmful Algal Blooms
    Aabir Banerji, Nichole E. Brinkman, Benjamin Davis, Alison Franklin, Michael Jahne, Scott P. Keely
    Microorganisms.2024; 12(11): 2121.     CrossRef
  • Extensive Genomic Rearrangement of Catalase-Less Cyanobloom-Forming Microcystis aeruginosa in Freshwater Ecosystems
    Minkyung Kim, Jaejoon Jung, Wonjae Kim, Yerim Park, Che Ok Jeon, Woojun Park
    Journal of Microbiology.2024; 62(11): 933.     CrossRef
  • Biological and Chemical Approaches for Controlling Harmful Microcystis Blooms
    Wonjae Kim, Yerim Park, Jaejoon Jung, Che Ok Jeon, Masanori Toyofuku, Jiyoung Lee, Woojun Park
    Journal of Microbiology.2024; 62(3): 249.     CrossRef
  • Alleviation of H2O2 toxicity by extracellular catalases in the phycosphere of Microcystis aeruginosa
    Yerim Park, Wonjae Kim, Yeji Cha, Minkyung Kim, Woojun Park
    Harmful Algae.2024; 137: 102680.     CrossRef
[MINIREVIEW]Gain and loss of antibiotic resistant genes in multidrug resistant bacteria: One Health perspective
Misung Kim , Jaeeun Park , Mingyeong Kang , Jihye Yang , Woojun Park
J. Microbiol. 2021;59(6):535-545.   Published online April 20, 2021
DOI: https://doi.org/10.1007/s12275-021-1085-9
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AbstractAbstract PDF
The emergence of multidrug resistance (MDR) has become a global health threat due to the increasing unnecessary use of antibiotics. Multidrug resistant bacteria occur mainly by accumulating resistance genes on mobile genetic elements (MGEs), made possible by horizontal gene transfer (HGT). Humans and animal guts along with natural and engineered environments such as wastewater treatment plants and manured soils have proven to be the major reservoirs and hotspots of spreading antibiotic resistance genes (ARGs). As those environments support the dissemination of MGEs through the complex interactions that take place at the human-animalenvironment interfaces, a growing One Health challenge is for multiple sectors to communicate and work together to prevent the emergence and spread of MDR bacteria. However, maintenance of ARGs in a bacterial chromosome and/or plasmids in the environments might place energy burdens on bacterial fitness in the absence of antibiotics, and those unnecessary ARGs could eventually be lost. This review highlights and summarizes the current investigations into the gain and loss of ARG genes in MDR bacteria among human-animal- environment interfaces. We also suggest alternative treatments such as combinatory therapies or sequential use of different classes of antibiotics/adjuvants, treatment with enzymeinhibitors, and phage therapy with antibiotics to solve the MDR problem from the perspective of One Health issues.

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    Bioresource Technology.2025; 415: 131704.     CrossRef
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    You Wang, Quanchao Cui, Yuliang Hou, Shunfu He, Wenxin Zhao, Zhuoma Lancuo, Kirill Sharshov, Wen Wang
    Veterinary Sciences.2025; 12(4): 297.     CrossRef
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    Microbiology Spectrum.2025;[Epub]     CrossRef
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    YanXing XU, Hao CHENG, XiaoJie HU, Chao QIN, YanZheng GAO
    SCIENTIA SINICA Technologica.2025; 55(4): 662.     CrossRef
  • Rapid Simultaneous Detection of the Clinically Relevant Carbapenemase Resistance Genes blaKPC, blaOXA48, blaVIM and blaNDM with the Newly Developed Ready-to-Use qPCR CarbaScan LyoBead
    Martin Reinicke, Celia Diezel, Salma Teimoori, Bernd Haase, Stefan Monecke, Ralf Ehricht, Sascha D. Braun
    International Journal of Molecular Sciences.2025; 26(3): 1218.     CrossRef
  • Mobile Genomic Island GEI-FN1A in Aeromonas salmonicida FN1 Contributes to the Spread of Antibiotic-Resistance Genes
    Xinru Xie, Zhaorui Ren, Rong Wang, Kanglu Tian, Xindi Huang, Yang Lyu, Guangxiang Cao, Jiafang Fu
    Current Microbiology.2025;[Epub]     CrossRef
  • Pathogens and drug resistance or virulence genes from animals and surrounding environment in Shenzhen, 2023 using targeted next-generation sequencing
    Dandan Niu, Yankun Ke, Qiuying Lv, Zhen Zhang, Tengyingzi Liu, Zhongyao Xu, Tong Li, Yingluan Zhang, Renli Zhang, Xiaomin Zhang, Shunping Cai, Xiaonan You, Yuqing Chen, Hanguang Wu, Yingxin Li, Chan Yin, Danzhu Wang, Xinhua Duan, Yongchao Guo, Rongqi Liu,
    Infection, Genetics and Evolution.2025; 131: 105755.     CrossRef
  • Bacterial cell wall synthesis and recycling: new antimicrobial targets and vaccine development
    Jihyeon Min, Bitnara Kim, Yerim Park, Yongjun Son, Woojun Park
    Critical Reviews in Microbiology.2025; 51(6): 1364.     CrossRef
  • Effects of antibiotics and heavy metals on antibiotic resistance genes and mobile gene elements in agricultural activity
    Xuanzi Guo, Xingxing Long, Jing Li, Jiangyan Wu, Xuran Zhu, Yanrong Zhu, Mingyang Weng, Yao Zhang
    Environmental Sciences Europe.2025;[Epub]     CrossRef
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    Gustavo A. Niño-Vega, Jorge A. Ortiz-Ramírez, Everardo López-Romero
    Antibiotics.2025; 14(4): 404.     CrossRef
  • Interactions between antibiotic resistance genes and soil environmental factors: Coupling, antagonism, and synergism
    Ling Wang, Xuefan Gu, Kunlong Hui, Tingqiao Yu, Ying Yuan, Gang Chen, Wenbing Tan
    Emerging Contaminants.2025; 11(4): 100578.     CrossRef
  • Antibiotic resistance genes in multi-matrices of Chaohu Lake: Spatiotemporal variation and correlation with pesticides and PPCPs
    Hao Yang, Hongwei Fu, Yutong Zhao, Run Li, Man Cao, Yi Liu, Yachao Ni, Wei Song, Yangyang Liang, Panyuan Cai, Wei Chang, Fangbiao Tao, Hua Wang, Kaiyong Liu
    Journal of Hazardous Materials.2025; 497: 139604.     CrossRef
  • Biofilm and Wastewater Dynamics in an activated-sludge process in the UK: Insights into microbiome composition, metabolic activity, and antimicrobial resistance using 16S rRNA sequencing
    Deborah Reid, John Craft, Ania Escudero, Colin Hunter, Janice Spencer
    Total Environment Microbiology.2025; 1(4): 100046.     CrossRef
  • Biodegradable microplastics amplify antibiotic resistance in aquaculture: A potential One Health crisis from environment to seafood
    Shiyu Xie, Licheng Peng, Zhenchao Zhou, Nuohan Xu, Siqi Li, Yujie Feng
    Journal of Hazardous Materials.2025; 498: 139932.     CrossRef
  • Investigation of the antimicrobial susceptibility patterns of marine cyanobacteria in Bohai Bay: Cyanobacteria may be important hosts of antibiotic resistance genes in marine environment
    Rui Xin, Ying Zhang, Kai Zhang, Yichen Yang, Yongzheng Ma, Zhiguang Niu
    Science of The Total Environment.2024; 909: 168516.     CrossRef
  • Hospital and municipal wastewater as a source of carbapenem-resistant Acinetobacter baumannii and Pseudomonas aeruginosa in the environment: a review
    Magdalena Męcik, Kornelia Stefaniak, Monika Harnisz, Ewa Korzeniewska
    Environmental Science and Pollution Research.2024; 31(36): 48813.     CrossRef
  • Unexpected vulnerability of Enterococcus faecium to polymyxin B under anaerobic condition
    Yongjun Son, Bitnara Kim, Pureun Kim, Jihyeon Min, Yerim Park, Jihye Yang, Wonjae Kim, Masanori Toyofuku, Woojun Park
    Gut Microbes.2024;[Epub]     CrossRef
  • Antimicrobial Resistance in the Context of Animal Production and Meat Products in Poland—A Critical Review and Future Perspective
    Patryk Wiśniewski, Miłosz Trymers, Wioleta Chajęcka-Wierzchowska, Katarzyna Tkacz, Anna Zadernowska, Monika Modzelewska-Kapituła
    Pathogens.2024; 13(12): 1123.     CrossRef
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    Pavithra Anantharaman Sudhakari, Bhaskar Chandra Mohan Ramisetty
    Microbial Drug Resistance.2024; 30(1): 37.     CrossRef
  • Transfer dynamics of antimicrobial resistance among gram-negative bacteria
    Bangjuan Wang, Muhammad Haris Raza Farhan, Linlin Yuan, Yuxin Sui, Jinhua Chu, Xiaohan Yang, Yuxin Li, Lingli Huang, Guyue Cheng
    Science of The Total Environment.2024; 954: 176347.     CrossRef
  • Pedobacter faecalis sp. nov., isolated from the faeces of eland, Taurotragus oryx
    Yerim Park, Jihyeon Min, Bitnara Kim, Woojun Park
    International Journal of Systematic and Evolutionary Microbiology .2024;[Epub]     CrossRef
  • Co-selection mechanism for bacterial resistance to major chemical pollutants in the environment
    Meixia Huo, Xiangyue Xu, Kun Mi, Wenjin Ma, Qin Zhou, Xudong Lin, Guyue Cheng, Lingli Huang
    Science of The Total Environment.2024; 912: 169223.     CrossRef
  • Repeated Exposure of Vancomycin to Vancomycin-Susceptible Staphylococcus aureus (VSSA) Parent Emerged VISA and VRSA Strains with Enhanced Virulence Potentials
    An Nguyen, J. Jean Sophy Roy, Ji-Hoon Kim, Kyung-Hee Yun, Wonsik Lee, Kyeong Kyu Kim, Truc Kim, Akhilesh Kumar Chaurasia
    Journal of Microbiology.2024; 62(7): 535.     CrossRef
  • Human Disturbance Increases Health Risks to Golden Snub-Nosed Monkeys and the Transfer Risk of Pathogenic Antibiotic-Resistant Bacteria from Golden Snub-Nosed Monkeys to Humans
    Shuzhen Zou, Tingting Yuan, Tan Lu, Jiayu Yan, Di Kang, Dayong Li
    Animals.2023; 13(19): 3083.     CrossRef
  • Longitudinal study of the short- and long-term effects of hospitalisation and oral trimethoprim-sulfadiazine administration on the equine faecal microbiome and resistome
    Mathijs J. P. Theelen, Roosmarijn E. C. Luiken, Jaap A. Wagenaar, Marianne M. Sloet van Oldruitenborgh-Oosterbaan, John W. A. Rossen, Femke J. W. C. Schaafstra, David A. van Doorn, Aldert L. Zomer
    Microbiome.2023;[Epub]     CrossRef
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    Danny Fuentes-Castillo, Daniela Castro-Tardón, Fernanda Esposito, Ingrith Neves, Larissa Rodrigues, Herrison Fontana, Bruna Fuga, José L. Catão-Dias, Nilton Lincopan
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    Pharmaceutics.2023; 15(7): 1782.     CrossRef
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  • Blue Light Sensing BlsA-Mediated Modulation of Meropenem Resistance and Biofilm Formation in Acinetobacter baumannii
    Jihye Yang, Sohyeon Yun, Woojun Park, Mark J. Mandel
    mSystems.2023;[Epub]     CrossRef
  • AamA-mediated epigenetic control of genome-wide gene expression and phenotypic traits in Acinetobacter baumannii ATCC 17978
    Jihye Yang, Yongjun Son, Mingyeong Kang, Woojun Park
    Microbial Genomics .2023;[Epub]     CrossRef
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  • The β-Lactamase Activity at the Community Level Confers β-Lactam Resistance to Bloom-Forming Microcystis aeruginosa Cells
    Yerim Park, Wonjae Kim, Minkyung Kim, Woojun Park
    Journal of Microbiology.2023; 61(9): 807.     CrossRef
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    Science of The Total Environment.2022; 811: 152331.     CrossRef
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    Karolina Jeżak, Anna Kozajda
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    Microbiology .2022;[Epub]     CrossRef
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Raman spectroscopy reveals alteration of spore compositions under different nutritional conditions in Lysinibacillus boronitolerans YS11
Youngung Ryu , Minyoung Hong , Soo Bin Kim , Tae Kwon Lee , Woojun Park
J. Microbiol. 2021;59(5):491-499.   Published online March 29, 2021
DOI: https://doi.org/10.1007/s12275-021-0679-6
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AbstractAbstract PDF
Little is known about final spores components when bacteria undergo sporulation under different nutrient conditions. Different degrees of resistance and germination rates were observed in the three types of spores of Lysinibacillus boronitolerans YS11 (SD, Spores formed in Difco sporulation mediumTM; SC and SF, Spores formed in an agricultural byproduct medium with 10 mM CaCl2 and with 10 mM FeSO4, respectively). Stronger UV resistance was recorded for SF with 1.8–2.3-fold greater survival than SC and SD under UV treatment. The three spore types showed similar heat resistances at 80°C, but survival rates of SC and SD were much higher (~1,000 times) than those of SF at 90°C. However, germination capacity of SF was 20% higher than those of SD and SC on Luria-Bertani agar plates for 24 h. SF germinated more rapidly in a liquid medium with high NaCl concentrations than SC and SD, but became slower under alkaline conditions. Raman spectroscopy was used to analyze the heterogeneities in the three types of vegetative cells and their spores under different nutritional conditions. Exponentially grown-each vegetative cells had different overall Raman peak values. Raman peaks of SC, SD, and SF also showed differences in adenine and amide III compositions and nucleic acid contents. Our data along with Raman spectroscopy provided the evidence that spores formed under under different growth conditions possess very different cellular components, which affected their survival and germination rates.

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    Harmful Algae.2024; 137: 102680.     CrossRef
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Optimization of bacterial sporulation using economic nutrient for self-healing concrete
Youngung Ryu , Ki-Eun Lee , In-Tae Cha , Woojun Park
J. Microbiol. 2020;58(4):288-296.   Published online February 27, 2020
DOI: https://doi.org/10.1007/s12275-020-9580-y
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AbstractAbstract PDF
The use of heat- and alkali-resistant bacteria is essential for the biological repair of damaged concrete. Lysinibacillus boronitolerans YS11 was isolated from the rhizosphere of Miscanthus sacchariflorus. The increased pH in the urea-minus condition during the growth of the YS11 strain promoted calcium carbonate (CaCO3) formation. To identify the optimum medium that promoted the growth of the YS11 strain, a Plackett- Burman design was conducted for the screening process. Consequently, malt powder, rice bran, (NH4)2SO4, and corn syrup were chosen to enhance YS11 growth. The optimization of these four useful factors was carried out using a central composite design. To obtain higher survivability in mortar, the sporulation process is essential, and additional factors such as Mn2+, Fe2+, and Ca2+ were found to contribute to sporulation. A mixture of L. boronitolerans YS11 spore powder, cement, paste, sand, yeast extract, calcium lactate, and water showed a healing effect on a 0.3 mm mortar crack in 7 days. Furthermore, calcium carbonate precipitation was observed over the crack surface. Thus, we confirmed that mortar treated with YS11 spore powder was effective in healing micro-cracks in concrete.

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Sterilization efficiency of pathogen-contaminated cottons in a laundry machine
Yoonjae Shin , Jungha Park , Woojun Park
J. Microbiol. 2020;58(1):30-38.   Published online November 25, 2019
DOI: https://doi.org/10.1007/s12275-020-9391-1
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AbstractAbstract PDF
Pathogenic bacteria on abiotic surfaces such as fabrics, bedding, patient wears, and surgical tools are known to increase the risk of bacterial diseases in infants and the elderly. The desiccation tolerance of bacteria affects their viability in cotton. Thus, washing and drying machines are required to use conditions that ensure the sterilization of bacteria in cotton. The objective of this study is to determine the effects of various sterilization conditions of washing and drying machines on the survival of three pathogenic bacteria (Acinetobacter baumannii, Pseudomonas aeruginosa, and Staphylococcus aureus) commonly presented in contaminated cotton and two non-pathogenic bacteria (Bacillus subtilis and Escherichia coli) in cotton. High survival rates of A. baumannii and S. aureus in desiccated cotton were observed based on scanning electron microscope and replicate organism direct agar contact assay. The survival rates of A. baumannii and S. aureus exposed in desiccated cotton for 8 h were higher (14.4 and 5.0%, respectively) than those of other bacteria (< 0.5%). All tested bacteria were eradicated at low-temperature (< 40°C) washing with activated oxygen bleach (AOB). However, bacterial viability was shown in low temperature washing without AOB. High-temperature (> 60°C) washing was required to achieve 99.9% of the sterilization rate in washing without AOB. The sterilization rate was 93.2% using a drying machine at 60°C for 4 h. This level of sterilization was insufficient in terms of time and energy efficiency. High sterilization efficiency (> 99.9%) at 75°C for 3 h using a drying machine was confirmed. This study suggests standard conditions of drying machines to remove bacterial contamination in cotton by providing practical data.

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EDITORIAL] Gut microbiomes and their metabolites shape human and animal health
Woojun Park
J. Microbiol. 2018;56(3):151-153.
DOI: https://doi.org/10.1007/s12275-018-0577-8
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AbstractAbstract PDF
The host genetic background, complex surrounding environments, and gut microbiome are very closely linked to human and animal health and disease. Although significant correlations between gut microbiota and human and animal health have been revealed, the specific roles of each gut bacterium in shaping human and animal health and disease remain unclear. However, recent omics-based studies using experimental animals and surveys of gut microbiota from unhealthy humans have provided insights into the relationships among microbial community, their metabolites, and human and animal health. This editorial introduces six review papers that provide new discoveries of disease-associated microbiomes and suggest possible microbiome-based therapeutic approaches to human disease.

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[Minireview] Antibiotic resistance of pathogenic Acinetobacter species and emerging combination therapy
Bora Shin , Woojun Park
J. Microbiol. 2017;55(11):837-849.   Published online October 27, 2017
DOI: https://doi.org/10.1007/s12275-017-7288-4
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AbstractAbstract PDF
The increasing antibiotic resistance of Acinetobacter species in both natural and hospital environments has become a serious problem worldwide in recent decades. Because of both intrinsic and acquired antimicrobial resistance (AMR) against last-resort antibiotics such as carbapenems, novel therapeutics are urgently required to treat Acinetobacter-associated infectious diseases. Among the many pathogenic Acinetobacter species, A. baumannii has been reported to be resistant to all classes of antibiotics and contains many AMR genes, such as blaADC (Acinetobacter-derived cephalosporinase). The AMR of pathogenic Acinetobacter species is the result of several different mechanisms, including active efflux pumps, mutations in antibiotic targets, antibiotic modification, and low antibiotic membrane permeability. To overcome the limitations of existing drugs, combination theraphy that can increase the activity of antibiotics should be considered in the treatment of Acinetobacter infections. Understanding the molecular mechanisms behind Acinetobacter AMR resistance will provide vital information for drug development and therapeutic strategies using combination treatment. Here, we summarize the classic mechanisms of Acinetobacter AMR, along with newly-discovered genetic AMR factors and currently available antimicrobial adjuvants that can enhance drug efficacy in the treatment of A. baumannii infections.

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Non-ureolytic calcium carbonate precipitation by Lysinibacillus sp. YS11 isolated from the rhizosphere of Miscanthus sacchariflorus
Yun Suk Lee , Hyun Jung Kim , Woojun Park
J. Microbiol. 2017;55(6):440-447.   Published online May 28, 2017
DOI: https://doi.org/10.1007/s12275-017-7086-z
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AbstractAbstract PDF
Although microbially induced calcium carbonate precipita-tion (MICP) through ureolysis has been widely studied in en-vironmental engineering fields, urea utilization might cause environmental problems as a result of ammonia and nitrate production. In this study, many non-ureolytic calcium car-bonate-precipitating bacteria that induced an alkaline envi-ronment were isolated from the rhizosphere of Miscanthus sacchariflorus near an artificial stream and their ability to pre-cipitate calcium carbonate minerals with the absence of urea was investigated. MICP was observed using a phase-contrast microscope and ion-selective electrode. Only Lysinibacillus sp. YS11 showed MICP in aerobic conditions. Energy disper-sive X-ray spectrometry and X-ray diffraction confirmed the presence of calcium carbonate. Field emission scanning elec-tron microscopy analysis indicated the formation of morpho-logically distinct minerals around cells under these condi-tions. Monitoring of bacterial growth, pH changes, and Ca2+ concentrations under aerobic, hypoxia, and anaerobic con-ditions suggested that strain YS11 could induce alkaline con-ditions up to a pH of 8.9 and utilize 95% of free Ca2+ only under aerobic conditions. Unusual Ca2+ binding and its re-lease from cells were observed under hypoxia conditions. Bio-film and extracellular polymeric substances (EPS) formation were enhanced during MICP. Strain YS11 has resistance at high pH and in high salt concentrations, as well as its spore- forming ability, which supports its potential application for self-healing concrete.

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MINIREVIEW] Indole: a signaling molecule or a mere metabolic byproduct that alters bacterial physiology at a high concentration?
Jisun Kim , Woojun Park
J. Microbiol. 2015;53(7):421-428.   Published online June 27, 2015
DOI: https://doi.org/10.1007/s12275-015-5273-3
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AbstractAbstract
Indole is an organic compound that is widespread in microbial communities inhabiting diverse habitats, like the soil environment and human intestines. Measurement of indole production is a traditional method for the identification of microbial species. Escherichia coli can produce millimolar concentrations of indole in the stationary growth phase under nutrient-rich conditions. Indole has received considerable attention because of its remarkable effects on various biological functions of the microbial communities, for example, biofilm formation, motility, virulence, plasmid stability, and antibiotic resistance. Indole may function as an intercellular signaling molecule, like a quorum-sensing signal. Nevertheless, a receptor system for indole and the function of this compound in coordinated behavior of a microbial population (which are requirements for a true signaling molecule) have not yet been confirmed. Recent findings suggest that a long-known quorum-sensing regulator, E. coli’s SdiA, cannot recognize indole and that this compound may simply cause membrane disruption and energy reduction, which can lead to various changes in bacterial physiology including unstable folding of a quorum-sensing regulator. Indole appears to be responsible for acquisition of antibiotic resistance via the formation of persister cells and activation of an exporter. This review highlights and summarizes the current knowledge about indole as a multitrophic molecule among bacteria, together with recently identified new avenues of research.

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Effects of Nutritional Input and Diesel Contamination on Soil Enzyme Activities and Microbial Communities in Antarctic Soils
Jiwon Han , Jaejoon Jung , Seunghun Hyun , Hyun Park , Woojun Park
J. Microbiol. 2012;50(6):916-924.   Published online December 30, 2012
DOI: https://doi.org/10.1007/s12275-012-2636-x
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Pollution of Antarctic soils may be attributable to increased nutritional input and diesel contamination via anthropogenic activities. To investigate the effect of these environmental changes on the Antarctic terrestrial ecosystem, soil enzyme activities and microbial communities in 3 types of Antarctic soils were evaluated. The activities of alkaline phosphomonoesterase and dehydrogenase were dramatically increased, whereas the activities of β-glucosidase, urease, arylsulfatase, and fluorescein diacetate hydrolysis were negligible. Alkaline phosphomonoesterase and dehydrogenase activities in the 3 types of soils increased 3- to 10-fold in response to nutritional input, but did not increase in the presence of diesel contamination. Consistent with the enzymatic activity data, increased copy numbers of the phoA gene, encoding an alkaline phosphomonoesterase, and the 16S rRNA gene were verified using quantitative real-time polymerase chain reaction. Interestingly, dehydrogenase activity and 16S rRNA gene copy number increased slightly after 30 days, even under diesel contamination, probably because of adaptation of the bacterial population. Intact Antarctic soils showed a predominance of Actinobacteria phylum (mostly Pseudonorcarida species) and other phyla such as Proteobacteria, Chloroflexi, Planctomycetes, Firmicutes, and Verrucomicrobia were present in successively lower proportions. Nutrient addition might act as a selective pressure on the bacterial community, resulting in the prevalence of Actinobacteria phylum (mostly Arthrobacter species). Soils contaminated by diesel showed a predominance of Proteobacteria phylum (mostly Phyllobacterium species), and other phyla such as Actinobacteria, Bacteroidetes, Planctomycetes, and Gemmatimonadetes were present in successively lower proportions. Our data reveal that nutritional input has a dramatic impact on bacterial communities in Antarctic soils and that diesel contamination is likely toxic to enzymes in this population.
NOTE] Pedobacter jeongneungensis sp. nov., Isolated from Forest Soil
Jaejoon Jung , Woojun Park
J. Microbiol. 2012;50(4):660-664.   Published online July 21, 2012
DOI: https://doi.org/10.1007/s12275-012-1629-0
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AbstractAbstract PDF
Strain BH45T was isolated from forest soil of Mt. Bukhan in Jeongneung, Seoul, Korea. The Gram-staining-negative strain BH45T grows at 4–30°C (optimum of 25–30°C) and between pH 5–8 (optimum of pH 6–8). Its major cellular fatty acids are C18:3 ω6c (6,9,12) and C10:0. The G+C content of genomic DNA was 40.2 mol%. The major respiratory quinone system in strain BH45T is menaquinone-7. Phylogenetic analysis based on 16S rRNA gene sequences indicates that strain BH45T is closely related to the genus Pedobacter. Sequence similarities with P. terrae KCTC 12762T, P. suwonensis KACC 11317T, P. soli KACC 14939T, P. alluvionis DSM 19624T, P. roseus KCCM 42272T, P. yonginense KCTC 22721T were 97.5, 97.1, 97.0, 97.0, 97.0, and 96.0%, respectively. DNA-DNA hybridization results distinguish strain BH45T from two Pedobacter species with high 16S rRNA gene sequence similarities. According to the phenotypic and molecular data, the strain BH45T clearly represents a novel species within the genus Pedobacter; thus, the name Pedobacter jeongneungensis sp. nov. is proposed for this strain. The type strain is BH45T (=KACC 15514T =JCM 17626T).

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Seasonal Changes in Nitrogen-Cycle Gene Abundances and in Bacterial Communities in Acidic Forest Soils
Jaejoon Jung , Jinki Yeom , Jiwon Han , Jisun Kim , Woojun Park
J. Microbiol. 2012;50(3):365-373.   Published online June 30, 2012
DOI: https://doi.org/10.1007/s12275-012-1465-2
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AbstractAbstract PDF
The abundance of genes related to the nitrogen biogeochemical cycle and the microbial community in forest soils (bacteria, archaea, fungi) were quantitatively analyzed via real-time PCR using 11 sets of specific primers amplifying nifH, bacterial amoA, archaeal amoA, narG, nirS, nirK, norB, nosZ, bacterial 16S rRNA gene, archaeal 16S rRNA gene, and the ITS sequence of fungi. Soils were sampled from Bukhan Mountain from September of 2010 to July of 2011 (7 times). Bacteria were the predominant microbial community in all samples. However, the abundance of archaeal amoA was greater than bacterial amoA throughout the year. The abundances of nifH, nirS, nirK, and norB genes changed in a similar pattern, while narG and nosZ appeared in sensitive to the environmental changes. Clone libraries of bacterial 16S rRNA genes were constructed from summer and winter soil samples and these revealed that Acidobacteria was the most predominant phylum in acidic forest soil environments in both samples. Although a specific correlation of environmental factor and gene abundance was not verified by principle component analysis, our data suggested that the combination of biological, physical, and chemical characteristics of forest soils created distinct conditions favoring the nitrogen biogeochemical cycle and that bacterial communities in undisturbed acidic forest soils were quite stable during seasonal change.

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Physiological and Metabolic Responses for Hexadecane Degradation in Acinetobacter oleivorans DR1
Jaejoon Jung , Jaemin Noh , Woojun Park
J. Microbiol. 2011;49(2):208-215.   Published online May 3, 2011
DOI: https://doi.org/10.1007/s12275-011-0395-8
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AbstractAbstract PDF
The hexadecane degradation of Acinetobacter oleivorans DR1 was evaluated with changes in temperature and ionic salt contents. Hexadecane degradation of strain DR1 was reduced markedly by the presence of sodium chloride (but not potassium chloride). High temperature (37°C) was also shown to inhibit the motility, biofilm formation, and hexadecane biodegradation. The biofilm formation of strain DR1 on the oil-water interface might prove to be a critical physiological feature for the degradation of hexadecane. The positive relationship between biofilm formation and hexadecane degradation could be observed at 30°C, but not at low temperatures (25°C). Alterations in cell hydrophobicity and EPS production by temperature and salts were not correlated with biofilm formation and hexadecane degradation. Our proteomic analyses have demonstrated that metabolic changes through the glyoxylate pathway are important for efficient degradation of hexadecane. Proteins involved in fatty acid metabolism, gluconeogenesis, and oxidative stress defense proteins appear to be highly expressed during biodegradation of hexadecane. These results suggested that biofilm formation and oxidative stress defense are important physiological responses for hexadecane degradation along with metabolic switch to glyoxylate pathway in strain DR1.
Acinetobacter oleivorans sp. nov. Is Capable of Adhering to and Growing on Diesel-Oil
Yoon-Suk Kang , Jaejoon Jung , Che Ok Jeon , Woojun Park
J. Microbiol. 2011;49(1):29-34.   Published online March 3, 2011
DOI: https://doi.org/10.1007/s12275-011-0315-y
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AbstractAbstract PDF
A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1T, was isolated from a rice paddy in Deok-So, South Korea. The strain DR1T cells were Gram-negative, aerobic coccobacilli, and grew at 20-37°C with the optimal temperature of 30°C, and an optimal pH of 6-8. Interestingly, strain DR1T was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 41.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1T and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1T in the genus Acinetobacter as a novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1T (=KCTC 23045T =JCM 16667T).

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    Water.2018; 10(4): 436.     CrossRef
  • Construction and Evaluation of a Korean Native Microbial Consortium for the Bioremediation of Diesel Fuel-Contaminated Soil in Korea
    Yunho Lee, Sang Eun Jeong, Moonsuk Hur, Sunghwan Ko, Che Ok Jeon
    Frontiers in Microbiology.2018;[Epub]     CrossRef
  • The natural environment as a reservoir of pathogenic and non-pathogenic Acinetobacter species
    Mary A. Adewoyin, Anthony I. Okoh
    Reviews on Environmental Health.2018; 33(3): 265.     CrossRef
  • Metabolic and stress responses of Acinetobacter oleivorans DR1 during long‐chain alkane degradation
    Chulwoo Park, Bora Shin, Jaejoon Jung, Yunho Lee, Woojun Park
    Microbial Biotechnology.2017; 10(6): 1809.     CrossRef
  • Acinetobacter halotolerans sp. nov., a novel halotolerant, alkalitolerant, and hydrocarbon degrading bacterium, isolated from soil
    Ram Hari Dahal, Dhiraj Kumar Chaudhary, Jaisoo Kim
    Archives of Microbiology.2017; 199(5): 701.     CrossRef
  • Phylogenetic signal in phenotypic traits related to carbon source assimilation and chemical sensitivity in Acinetobacter species
    Ado Van Assche, Sergio Álvarez-Pérez, Anna de Breij, Joseph De Brabanter, Kris A. Willems, Lenie Dijkshoorn, Bart Lievens
    Applied Microbiology and Biotechnology.2017; 101(1): 367.     CrossRef
  • Screening and In Situ Monitoring of Potential Petroleum Hydrocarbon Degraders in Contaminated Surface Water
    Bulent Icgen, Fadime Yilmaz
    CLEAN – Soil, Air, Water.2017;[Epub]     CrossRef
  • Inhibitory Effect of Taurine on Biofilm Formation During Alkane Degradation in Acinetobacter oleivorans DR1
    Hyo Jung Eom, Woojun Park
    Microbial Ecology.2017; 74(4): 821.     CrossRef
  • Resistance to Antibiotics, Biocides, Preservatives and Metals in Bacteria Isolated from Seafoods: Co-Selection of Strains Resistant or Tolerant to Different Classes of Compounds
    José L. Romero, María J. Grande Burgos, Rubén Pérez-Pulido, Antonio Gálvez, Rosario Lucas
    Frontiers in Microbiology.2017;[Epub]     CrossRef
  • Quorum sensing network in clinical strains of A. baumannii: AidA is a new quorum quenching enzyme
    María López, Celia Mayer, Laura Fernández-García, Lucía Blasco, Andrea Muras, Federico Martín Ruiz, German Bou, Ana Otero, María Tomás, Feng Gao
    PLOS ONE.2017; 12(3): e0174454.     CrossRef
  • Hydrocarbon-Degrading Bacteria Exhibit a Species-Specific Response to Dispersed Oil while Moderating Ecotoxicity
    Will A. Overholt, Kala P. Marks, Isabel C. Romero, David J. Hollander, Terry W. Snell, Joel E. Kostka, G. Voordouw
    Applied and Environmental Microbiology.2016; 82(2): 518.     CrossRef
  • Acinetobacter lactucae sp. nov., isolated from iceberg lettuce (Asteraceae: Lactuca sativa)
    Alejandro P. Rooney, Christopher A. Dunlap, Lina B. Flor-Weiler
    International Journal of Systematic and Evolutionary Microbiology.2016; 66(9): 3566.     CrossRef
  • Reservoirs of Non-baumannii Acinetobacter Species
    Ahmad Al Atrouni, Marie-Laure Joly-Guillou, Monzer Hamze, Marie Kempf
    Frontiers in Microbiology.2016;[Epub]     CrossRef
  • Community Analysis and Recovery of Phenol-degrading Bacteria from Drinking Water Biofilters
    Qihui Gu, Qingping Wu, Jumei Zhang, Weipeng Guo, Huiqing Wu, Ming Sun
    Frontiers in Microbiology.2016;[Epub]     CrossRef
  • Acinetobacter species as model microorganisms in environmental microbiology: current state and perspectives
    Jaejoon Jung, Woojun Park
    Applied Microbiology and Biotechnology.2015; 99(6): 2533.     CrossRef
  • Molecular Mechanisms of Enhanced Bacterial Growth on Hexadecane with Red Clay
    Jaejoon Jung, In-ae Jang, Sungeun Ahn, Bora Shin, Jisun Kim, Chulwoo Park, Seung Cheol Jee, Jung-Suk Sung, Woojun Park
    Microbial Ecology.2015; 70(4): 912.     CrossRef
  • Diesel Oil Degradation Potential of a Bacterium Inhabiting Petroleum Hydrocarbon Contaminated Surface Waters and Characterization of Its Emulsification Ability
    Gozde Onur, Fadime Yilmaz, Bulent Icgen
    Journal of Surfactants and Detergents.2015; 18(4): 707.     CrossRef
  • Role of quorum sensing in bacterial infections
    Israel Castillo-Juárez, Toshinari Maeda, Edna Ayerim Mandujano-Tinoco, María Tomás, Berenice Pérez-Eretza, Silvia Julieta García-Contreras, Thomas K Wood, Rodolfo García-Contreras
    World Journal of Clinical Cases.2015; 3(7): 575.     CrossRef
  • TetR repressor-based bioreporters for the detection of doxycycline using Escherichia coli and Acinetobacter oleivorans
    Hyerim Hong, Woojun Park
    Applied Microbiology and Biotechnology.2014; 98(11): 5039.     CrossRef
  • Plasmid-Encoded Tetracycline Efflux Pump Protein Alters Bacterial Stress Responses and Ecological Fitness of Acinetobacter oleivorans
    Hyerim Hong, Jaejoon Jung, Woojun Park, Nancy E. Freitag
    PLoS ONE.2014; 9(9): e107716.     CrossRef
  • Acinetobacter nectaris sp. nov. and Acinetobacter boissieri sp. nov., isolated from floral nectar of wild Mediterranean insect-pollinated plants
    Sergio Álvarez-Pérez, Bart Lievens, Hans Jacquemyn, Carlos M. Herrera
    International Journal of Systematic and Evolutionary Microbiology.2013; 63(Pt_4): 1532.     CrossRef
  • The genome sequence of the hydrocarbon-degrading Acinetobacter venetianus VE-C3
    Marco Fondi, Ermanno Rizzi, Giovanni Emiliani, Valerio Orlandini, Luisa Berna, Maria Cristiana Papaleo, Elena Perrin, Isabel Maida, Giorgio Corti, Gianluca De Bellis, Franco Baldi, Lenie Dijkshoorn, Mario Vaneechoutte, Renato Fani
    Research in Microbiology.2013; 164(5): 439.     CrossRef
  • Evolution of a Pathogen: A Comparative Genomics Analysis Identifies a Genetic Pathway to Pathogenesis in Acinetobacter
    Jason W. Sahl, John D. Gillece, James M. Schupp, Victor G. Waddell, Elizabeth M. Driebe, David M. Engelthaler, Paul Keim, J. Ross Fitzgerald
    PLoS ONE.2013; 8(1): e54287.     CrossRef
  • Characterization of novel diesel-degrading strains Acinetobacter haemolyticus MJ01 and Acinetobacter johnsonii MJ4 isolated from oil-contaminated soil
    Myungjin Lee, Sung-Geun Woo, Leonid N. Ten
    World Journal of Microbiology and Biotechnology.2012; 28(5): 2057.     CrossRef
  • Diverse responses to UV light exposure in Acinetobacter include the capacity for DNA damage-induced mutagenesis in the opportunistic pathogens Acinetobacter baumannii and Acinetobacter ursingii
    Janelle M. Hare, James A. Bradley, Ching-li Lin, Tyler J. Elam
    Microbiology.2012; 158(3): 601.     CrossRef
  • Complexity of cell–cell interactions between Pseudomonas sp. AS1 and Acinetobacter oleivorans DR1: metabolic commensalism, biofilm formation and quorum quenching
    Hyoju Seo, Jisun Kim, Jaejoon Jung, Hyun Mi Jin, Che Ok Jeon, Woojun Park
    Research in Microbiology.2012; 163(3): 173.     CrossRef
  • Acinetobacter: A potential reservoir and dispenser for β-lactamases
    Wei-Hua Zhao, Zhi-Qing Hu
    Critical Reviews in Microbiology.2012; 38(1): 30.     CrossRef
  • Physiological and metabolic responses for hexadecane degradation in Acinetobacter oleivorans DR1
    Jaejoon Jung, Jaemin Noh, Woojun Park
    The Journal of Microbiology.2011; 49(2): 208.     CrossRef
  • Comparative Genomic Analysis of Acinetobacter oleivorans DR1 To Determine Strain-Specific Genomic Regions and Gentisate Biodegradation
    Jaejoon Jung, Eugene L. Madsen, Che Ok Jeon, Woojun Park
    Applied and Environmental Microbiology.2011; 77(20): 7418.     CrossRef
NtrC-Sensed Nitrogen Availability Is Important for Oxidative Stress Defense in Pseudomonas putida KT2440
Sujin Yeom , Jinki Yeom , Woojun Park
J. Microbiol. 2010;48(2):153-159.   Published online May 1, 2010
DOI: https://doi.org/10.1007/s12275-010-0075-0
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  • 15 Scopus
AbstractAbstract PDF
The zwf, which encodes glucose-6-phosphate dehydrogenase, is repressed by NtrC under nitrogen-limited condition. Previously, we demonstrated that induction of zwf-1 is required for protecting Pseudomonas putida cells under oxidative stress, which could be possible probably because of derepression of HexR on the zwf-1 gene under oxidative stress. These findings led us investigate that NtrC still represses the zwf-1 under nitrogen-limited oxidative stress condition, which makes cells more sensitive under such condition. Interestingly, deletion of the ntrC gene significantly reduces growth rate, but renders cells more resistant to oxidative stress, under nitrogen limited condition in P. putida. More vitality of the ntrC mutant under oxidative stress condition was also confirmed by the fluorogenic redox dye using flow cytometry. The results of transcriptome analysis demonstrated that the derepression of several oxidative stress genes along with the zwf-1 gene might confer high resistance to oxidative stress in the ntrC mutant. Here, we presented the data for the first time, showing that different sets of genes are involved in nitrogen-rich and nitrogen-limited oxidative stress conditions and NtrC-sensed nitrogen availability is one of the most important prerequisite for full cellular defense against oxidative stress in P. putida.
Analysis of Microbial Communities Using Culture-dependent and Culture-independent Approaches in an Anaerobic/Aerobic SBR Reactor
Shipeng Lu , Minjeong Park , Hyeon-Su Ro , Dae Sung Lee , Woojun Park , Che Ok Jeon
J. Microbiol. 2006;44(2):155-161.
DOI: https://doi.org/2370 [pii]
  • 235 View
  • 2 Download
AbstractAbstract PDF
Comparative analysis of microbial communities in a sequencing batch reactor which performed enhanced biological phosphorus removal (EBPR) was carried out using a cultivation-based technique and 16S rRNA gene clone libraries. A standard PCR protocol and a modified PCR protocol with low PCR cycle was applied to the two clone libraries of the 16S rRNA gene sequences obtained from EBPR sludge, respectively, and the resulting 424 clones were analyzed using restriction fragment length polymorphisms (RFLPs) on 16S rRNA gene inserts. Comparison of two clone libraries showed that the modified PCR protocol decreased the incidence of distinct fragment patterns from about 63% (137 of 217) in the standard PCR method to about 34% (70 of 207) under the modified protocol, suggesting that just a low level of PCR cycling (5 cycles after 15 cycles) can significantly reduce the formation of chimeric DNA in the final PCR products. Phylogenetic analysis of 81 groups with distinct RFLP patterns that were obtained using the modified PCR method revealed that the clones were affiliated with at least 11 phyla or classes of the domain Bacteria. However, the analyses of 327 colonies, which were grouped into just 41 distinct types by RFLP analysis, showed that they could be classified into five major bacterial lineages: α, β, γ- Proteobacteria, Actinobacteria, and the phylum Bacteroidetes, which indicated that the microbial community yielded from the cultivationbased method was still much simpler than that yielded from the PCR-based molecular method. In this study, the discrepancy observed between the communities obtained from PCR-based and cultivation-based methods seems to result from low culturabilities of bacteria or PCR bias even though modified culture and PCR methods were used. Therefore, continuous development of PCR protocol and cultivation techniques is needed to reduce this discrepancy.
Woojun Park 2 Articles
Extensive Genomic Rearrangement of Catalase-Less Cyanobloom-Forming Microcystis aeruginosa in Freshwater Ecosystems
Minkyung Kim, Jaejoon Jung, Wonjae Kim, Yerim Park, Che Ok Jeon, Woojun Park
J. Microbiol. 2024;62(11):933-950.   Published online October 8, 2024
DOI: https://doi.org/10.1007/s12275-024-00172-7
  • 495 View
  • 10 Download
  • 3 Web of Science
  • 3 Crossref
AbstractAbstract PDF
Many of the world's freshwater ecosystems suffer from cyanobacteria-mediated blooms and their toxins. However, a mechanistic understanding of why and how Microcystis aeruginosa dominates over other freshwater cyanobacteria during warmer summers is lacking. This paper utilizes comparative genomics with other cyanobacteria and literature reviews to predict the gene functions and genomic architectures of M. aeruginosa based on complete genomes. The primary aim is to understand this species' survival and competitive strategies in warmer freshwater environments. M. aeruginosa strains exhibiting a high proportion of insertion sequences (~ 11%) possess genomic structures with low synteny across different strains. This indicates the occurrence of extensive genomic rearrangements and the presence of many possible diverse genotypes that result in greater population heterogeneities than those in other cyanobacteria in order to increase survivability during rapidly changing and threatening environmental challenges. Catalase-less M. aeruginosa strains are even vulnerable to low light intensity in freshwater environments with strong ultraviolet radiation. However, they can continuously grow with the help of various defense genes (e.g., egtBD, cruA, and mysABCD) and associated bacteria. The strong defense strategies against biological threats (e.g., antagonistic bacteria, protozoa, and cyanophages) are attributed to dense exopolysaccharide (EPS)-mediated aggregate formation with efficient buoyancy and the secondary metabolites of M. aeruginosa cells. Our review with extensive genome analysis suggests that the ecological vulnerability of M. aeruginosa cells can be overcome by diverse genotypes, secondary defense metabolites, reinforced EPS, and associated bacteria.

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  • Cyanophage Infections in a Sponge Intracellular Cyanobacterial Symbiont
    Tzipora Peretz, Esther Cattan‐Tsaushu, Chiara Conti, Benyamin Rosental, Laura Steindler, Sarit Avrani
    Environmental Microbiology.2025;[Epub]     CrossRef
  • Public goods-mediated bacterial interplay in aquatic ecosystems
    Yerim Park, Wonjae Kim, Jihye Bae, Woojun Park
    Water Research.2025; 287: 124310.     CrossRef
  • Horizontal Gene Transfer and Recombination in Cyanobacteriota
    Devaki Bhaya, Gabriel Birzu, Eduardo P.C. Rocha
    Annual Review of Microbiology .2025; 79(1): 685.     CrossRef
Biological and Chemical Approaches for Controlling Harmful Microcystis Blooms
Wonjae Kim, Yerim Park, Jaejoon Jung, Che Ok Jeon, Masanori Toyofuku, Jiyoung Lee, Woojun Park
J. Microbiol. 2024;62(3):249-260.   Published online April 8, 2024
DOI: https://doi.org/10.1007/s12275-024-00115-2
  • 703 View
  • 21 Download
  • 14 Web of Science
  • 13 Crossref
AbstractAbstract PDF
The proliferation of harmful cyanobacterial blooms dominated by Microcystis aeruginosa has become an increasingly serious problem in freshwater ecosystems due to climate change and eutrophication. Microcystis-blooms in freshwater generate compounds with unpleasant odors, reduce the levels of dissolved O2, and excrete microcystins into aquatic ecosystems, potentially harming various organisms, including humans. Various chemical and biological approaches have thus been developed to mitigate the impact of the blooms, though issues such as secondary pollution and high economic costs have not been adequately addressed. Red clays and H2O2 are conventional treatment methods that have been employed worldwide for the mitigation of the blooms, while novel approaches, such as the use of plant or microbial metabolites and antagonistic bacteria, have also recently been proposed. Many of these methods rely on the generation of reactive oxygen species, the inhibition of photosynthesis, and/or the disruption of cellular membranes as their mechanisms of action, which may also negatively impact other freshwater microbiota. Nevertheless, the underlying molecular mechanisms of anticyanobacterial chemicals and antagonistic bacteria remain unclear. This review thus discusses both conventional and innovative approaches for the management of M. aeruginosa in freshwater bodies.

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    Ecological Engineering.2025; 212: 107543.     CrossRef
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    Toxins.2025; 17(7): 354.     CrossRef
  • Public goods-mediated bacterial interplay in aquatic ecosystems
    Yerim Park, Wonjae Kim, Jihye Bae, Woojun Park
    Water Research.2025; 287: 124310.     CrossRef
  • Molecular mechanisms underlying the bloom-forming cyanobacterium Microcystis defence against predation by flagellates
    Yan Chen, Xiao Zhang, Xinyang Bai, Yonglan Xu, Hangzhou Xu, Li Li
    Journal of Water Process Engineering.2025; 77: 108611.     CrossRef
  • Utilizing allelopathy from the invasive plant Solidago canadensis to control Microcystis aeruginosa blooms: An integrated metabolomic and bioassay approach
    Miao Wu, Huiyuan Liu, Jiaxin Shen, Zhaohui Xie, Siyuan Yang, Jiahui Guo, Yijiang Liu, Huiting Lian, Dingli Wang
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    Sensors.2024; 24(13): 4350.     CrossRef
  • Alleviation of H2O2 toxicity by extracellular catalases in the phycosphere of Microcystis aeruginosa
    Yerim Park, Wonjae Kim, Yeji Cha, Minkyung Kim, Woojun Park
    Harmful Algae.2024; 137: 102680.     CrossRef
  • Extensive Genomic Rearrangement of Catalase-Less Cyanobloom-Forming Microcystis aeruginosa in Freshwater Ecosystems
    Minkyung Kim, Jaejoon Jung, Wonjae Kim, Yerim Park, Che Ok Jeon, Woojun Park
    Journal of Microbiology.2024; 62(11): 933.     CrossRef
  • Laboratory-Simulated Inhibitory Effects of the Floating-Bed Plants on Microcystis aeruginosa and Their Microbial Communities’ Responses to Microcystins
    Shuwen Zhang, Yuanpu Sha, Yuanyuan Tang, Longjie Li, Feihu Wang, Jing Dong, Xuejun Li, Yunni Gao, Xiaofei Gao, Huatao Yuan, Jingxiao Zhang
    Microorganisms.2024; 12(10): 2035.     CrossRef
  • Host-Associated Microbiome
    Woo Jun Sul
    Journal of Microbiology.2024; 62(3): 135.     CrossRef

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