- Reversible function of RapA with the C-terminus of RapC in Dictyostelium
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Dongju Kim , Wonbum Kim , Taeck Joong Jeon
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J. Microbiol. 2021;59(9):853-848.
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DOI: https://doi.org/10.1007/s12275-021-1400-5
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Rap small GTPases are involved in diverse signaling pathways
associated with cell growth, proliferation, and cell migration.
There are three Rap proteins in Dictyostelium, RapA, RapB,
and RapC. RapA is a key regulator in the control of cell adhesion
and migration. Recently RapA and RapC have been
reported to have opposite functions in the regulation of cellular
processes. In this study, we demonstrate that the C-terminus
of RapC, which is not found in RapA, is essential for
the opposite functions of RapC and is able to reverse the functions
of RapA when fused to the tail of RapA. Cells lacking
RapC displayed several defective phenotypes, including spread
morphology, strong adhesion, and decreased cell migration
compared to wild-type cells. These phenotypes were rescued
by full-length RapC, but not by RapC missing the C-terminus.
Furthermore, recombinant RapA fused with the C-terminus
of RapC completely recovered the phenotypes of rapC
null cells, indicating that the functions of RapA were modified
to become similar to those of RapC by the C-terminus of
RapC with respect to cell morphology, cell adhesion and migration,
cytokinesis, and development. These results suggest
that the C-terminal residues of RapC are able to suppress and
change the functions of other Ras proteins in Ras oncogenic
signaling pathways.
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Citations
Citations to this article as recorded by  -
Nhe1 is required for directional sensing in vegetative
Dictyostelium
cell migration
Uri Han, Nara Han, Taeck Joong Jeon
Cell Adhesion & Migration.2025;[Epub] CrossRef - RapB Regulates Cell Adhesion and Migration in Dictyostelium, Similar to RapA
Uri Han, Nara Han, Byeonggyu Park, Taeck Joong Jeon
Journal of Microbiology.2024; 62(8): 627. CrossRef - Adhesion of Dictyostelium Amoebae to Surfaces: A Brief History of Attachments
Lucija Mijanović, Igor Weber
Frontiers in Cell and Developmental Biology.2022;[Epub] CrossRef
- WasC, a WASP family protein, is involved in cell adhesion and migration through regulation of F-actin polymerization in Dictyostelium
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Pyeonghwa Jeon , Taeck Joong Jeon
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J. Microbiol. 2020;58(8):696-702. Published online June 10, 2020
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DOI: https://doi.org/10.1007/s12275-020-0138-9
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372
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The actin cytoskeleton is involved in the regulation of cell
morphology and migration. Wiskott-Aldrich Syndrome proteins
(WASPs) play an important role in controlling actin
polymerization by activating the Arp2/3 complex. The present
study investigated the roles of WasC, one of the 3 WASPs
in Dictyostelium, in cellular processes. Cells lacking WasC
displayed strong cell adhesion and approximately 1.5-fold
increase in F-actin levels as compared to the wild-type cells.
Loss of wasC caused defects in phagocytosis and decreased
the migration speed in chemoattractant-mediated cell migration
but did not affect directionality. WasC was localized to the
protruding region in migrating cells and, transiently and rapidly
translocated to the cell cortex in response to chemoattractant
stimulation, in an F-actin dependent manner. Our
results
suggest that WasC is involved in cell adhesion and
migration by regulating F-actin polymerization at the leading
edge of migrating cells, probably as a negative regulator.
The increased strength of adhesion in wasC null cells is likely
to decrease the migration speed but not the directionality.
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Citations
Citations to this article as recorded by - Wiskott-Aldrich syndrome gene as a prognostic biomarker correlated with immune infiltrates in clear cell renal cell carcinoma
Guixin Ding, Tianqi Wang, Shangjing Liu, Zhongbao Zhou, Jian Ma, Jitao Wu
Frontiers in Immunology.2023;[Epub] CrossRef - Dual regulation of the actin cytoskeleton by CARMIL-GAP
Goeh Jung, Miao Pan, Christopher J. Alexander, Tian Jin, John A. Hammer
Journal of Cell Science.2022;[Epub] CrossRef - Regulation of the Actin Cytoskeleton via Rho GTPase Signalling in Dictyostelium and Mammalian Cells: A Parallel Slalom
Vedrana Filić, Lucija Mijanović, Darija Putar, Antea Talajić, Helena Ćetković, Igor Weber
Cells.2021; 10(7): 1592. CrossRef
- Loss of FrmB results in increased size of developmental structures during the multicellular development of Dictyostelium cells
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Hyeseon Kim , Mi-Rae Lee , Taeck Joong Jeon
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J. Microbiol. 2017;55(9):730-736. Published online September 2, 2017
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DOI: https://doi.org/10.1007/s12275-017-7221-x
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308
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FERM domain-containing proteins are involved in diverse
biological and pathological processes, including cell-substrate
adhesion, cell-cell adhesion, multicellular development,
and cancer metastasis. In this study, we determined the functions
of FrmB, a FERM domain-containing protein, in the
cell morphology, cell adhesion, and multicellular development
of Dictyostelium cells. Our results show that FrmB appears
to play an important role in regulating the size of developmental
structures. frmB null cells showed prolonged aggregation
during development, resulting in increased size of developmental
structures, such as mounds and fruiting bodies,
compared to those of wild-type cells, whereas FrmB overexpressing
cells exhibited decreased size of developmental
structures. These results suggest that FrmB may be necessary
for limiting the sizes of developmental structures. Loss of
FrmB also resulted in decreased cell-substrate adhesion and
slightly increased cell area, suggesting that FrmB had important
roles in the regulation of cell adhesion and cell morphology.
These studies would contribute to our understanding
of the intertwined and overlapped functions of FERM
domain-containing proteins.
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Citations
Citations to this article as recorded by - The IQGAP-related RasGAP IqgC regulates cell–substratum adhesion in Dictyostelium discoideum
Lucija Mijanović, Darija Putar, Lucija Mimica, Sabina Klajn, Vedrana Filić, Igor Weber
Cellular & Molecular Biology Letters.2025;[Epub] CrossRef - Adhesion of Dictyostelium Amoebae to Surfaces: A Brief History of Attachments
Lucija Mijanović, Igor Weber
Frontiers in Cell and Developmental Biology.2022;[Epub] CrossRef - Opposite functions of RapA and RapC in cell adhesion and migration in Dictyostelium
Jihyeon Jeon, Dongju Kim, Taeck Joong Jeon
Animal Cells and Systems.2021; 25(4): 203. CrossRef
- Minimal amino acids in the I/LWEQ domain required for anterior/posterior localization in Dictyostelium
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Hyeseon Kim , Dong-Yeop Shin , Taeck Joong Jeon
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J. Microbiol. 2017;55(5):366-372. Published online January 26, 2017
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DOI: https://doi.org/10.1007/s12275-017-6550-0
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Establishment of cell polarity is mediated by a series of signal-ing molecules that are asymmetrically activated or localized in the cell upon extracellular stimulation. To understand the mechanism that mediates anterior/posterior asymmetric localization of RapGAP3 during migration, we determined the minimally required amino acids in the I/LWEQ domain that cause posterior localization and found that the minimal region of the F-actin binding domain for posterior localiza-tion could, with some additional deletion at the C-terminal, localize to the anterior. Analysis of the localization and trans-location kinetics to the cell cortex of the truncated proteins suggests that the required regions for anterior/posterior lo-calization might have a preferential binding affinity to pre- existing F-actins at the rear and lateral sides of the cell or newly formed F-actins at the front of the cell, leading to dis-tinct differential sites of the cell.
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