- Descr!ption of Ornithinimicrobium cryptoxanthini sp. nov., a Novel Actinomycete Producing β‑cryptoxanthin Isolated from the Tongtian River Sediments
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Yuyuan Huang , Yifan Jiao , Sihui Zhang , Yuanmeihui Tao , Suping Zhang , Dong Jin , Ji Pu , Liyun Liu , Jing Yang , Shan Lu
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J. Microbiol. 2023;61(4):379-388. Published online March 16, 2023
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DOI: https://doi.org/10.1007/s12275-023-00029-5
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 Abstract
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Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and
JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37′13″N, 96°05′37″E)
in July 2019. Both strains were shown to grow at 15–35 °C and pH 7.0–10.0, and in the presence of 0–6.0% (w/v) NaCl.
The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH
30183
T (98.6–98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T
(98.5–98.6%) and O. murale 01-Gi-040T
(98.3–98.5%). The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively,
revealed that the two strains formed a distinct cluster with the above three species. The digital DNA-DNA hybridization
(dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other
Ornithinimicrobium species were within the ranges of 19.0–23.9% and 70.8–80.4%, respectively, all below the respective
recommended 70.0% and 95–96% cutoff point. Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and
JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9. Strain JY.X270T contained MK-8(H4) and ornithine as the predominant
menaquinone and diagnostic diamino acid component within the cell wall teichoic acids. β-cryptoxanthin (
C40H56O) can
be extracted from strain JY.X270T, and its content is 6.3 μg/ml. Based on results from the phylogenetic, chemotaxonomic,
and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which
the name Ornithinimicrobium cryptoxanthini sp. nov. is proposed (type strain JY.X270T = CGMCC 1.19147T = JCM 34882T).
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Citations
Citations to this article as recorded by  - Screening, identification, and characterization of high potential bacteria for ꞵ-cryptoxanthin production from natural sources
Sopida Korkerd, Savitri Vatanyoopaisarn, Wonnop Visessaguan, Benjawan Thumthanarak, Dudsadee Uttapap, Solange I. Mussatto, Vilai Rungsardthong
Biocatalysis and Agricultural Biotechnology.2024; 57: 103089. CrossRef
- Changpingibacter yushuensis gen. nov., sp. nov., isolated from fluvial sediment in Qinghai Tibet Plateau of China
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Yifan Jiao , Sihui Zhang , Jing Yang , Xin-He Lai , Kui Dong , Yanpeng Cheng , Mingchao Xu , Wentao Zhu , Shan Lu , Dong Jin , Ji Pu , Ying Huang , Liyun Liu , Suping Wang , Jianguo Xu
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J. Microbiol. 2022;60(2):147-155. Published online January 7, 2022
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DOI: https://doi.org/10.1007/s12275-022-1199-8
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Abstract
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Two facultatively anaerobic, short rod-shaped, non-motile,
Gram-stain-positive, unknown bacterial strains (JY-X040T
and JY-X174) were isolated from fluvial sediments of Tongtian
River in Yushu Tibetan Autonomous Prefecture, Qinghai
province, China. Cells formed translucent, gray, round and
convex colonies, with a diameter of less than 0.5 mm after 5
days of incubation at 30°C on brain heart infusion-5% sheep
blood agar. The 16S rRNA gene sequence similarity between
strain JY-X040T and Fudania jinshanensis 313T is 93.87%.
In the four phylogenetic trees constructed based on the 16S
rRNA gene and 423 core genes, the two isolates form an independent
branch, phylogenetically closest to F. jinshanensis
313T, but could not be classified as a member of the genus
Fudania or any other genus of the family Arcanobacteriaceae.
The DNA G + C content of strain JY-X040T was 57.8%. Calculation
results
of average nucleotide identity, digital DNADNA
hybridization value and amino acid identity between
strain JY-X040T and F. jinshanensis 313T are 69.9%, 22.9%,
and 64.1%. The major cellular fatty acids were C16:0 (23%)
and C18:1ω9c (22%). The cell-wall peptidoglycan type was A5α
(L-Lys-L-Ala-L-Lys-D-Glu). The polar lipids comprised diphosphatidylglycerol,
phosphatidylglycerol, phosphatidylinositol,
phosphatidylinositol mannoside and four unidentified components.
The whole-cell sugars contained rhamnose and ribose.
MK-10(H4) was the sole respiratory quinone. The minimum
inhibitory concentration of streptomycin was 32 μg/ml. All
physiological, biochemical, chemotaxonomic and genomic
characteristics support that strains JY-X040T and JY-X174
represent members of a novel species in a new genus, Changpingibacter
yushuensis gen. nov., sp. nov. The type strain is
JY-X040T (GDMCC 1.1996T = KCTC 49514T).
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Citations
Citations to this article as recorded by - Metagenomic and Culturomics Analysis of Microbial Communities within Surface Sediments and the Prevalence of Antibiotic Resistance Genes in a Pristine River: The Zaqu River in the Lancang River Source Region, China
Yi Yan, Jialiang Xu, Wenmin Huang, Yufeng Fan, Zhenpeng Li, Mingkai Tian, Jinsheng Ma, Xin Lu, Jian Liang
Microorganisms.2024; 12(5): 911. CrossRef - Bacterial diversity in arboreal ant nesting spaces is linked to colony developmental stage
Maximilian Nepel, Veronika E. Mayer, Veronica Barrajon-Santos, Dagmar Woebken
Communications Biology.2023;[Epub] CrossRef
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