- Characterization of a Salmonella Enteritidis bacteriophage showing broad lytic activity against Gram-negative enteric bacteria
-
Shukho Kim , Sung-Hun Kim , Marzia Rahman , Jungmin Kim
-
J. Microbiol. 2018;56(12):917-925. Published online October 25, 2018
-
DOI: https://doi.org/10.1007/s12275-018-8310-1
-
-
82
View
-
0
Download
-
24
Crossref
-
 Abstract
-
In this study, we sought to isolate Salmonella Enteritidis-specific
lytic bacteriophages (phages), and we found a lytic phage
that could lyse not only S. Enteritidis but also other Gramnegative
foodborne pathogens. This lytic phage, SS3e, could
lyse almost all tested Salmonella enterica serovars as well as
other enteric pathogenic bacteria including Escherichia coli,
Shigella sonnei, Enterobacter cloacae, and Serratia marcescens.
This SS3e phage has an icosahedral head and a long tail, indicating
belong to the Siphoviridae. The genome was 40,793
base pairs, containing 58 theoretically determined open reading
frames (ORFs). Among the 58 ORFs, ORF49, and ORF25
showed high sequence similarity with tail spike protein and
lysozyme-like protein of Salmonella phage SE2, respectively,
which are critical proteins recognizing and lysing host bacteria.
Unlike SE2 phage whose host restricted to Salmonella
enterica serovars Enteritidis and Gallinarum, SS3e showed
broader host specificity against Gram-negative enteric bacteria;
thus, it could be a promising candidate for the phage
utilization against various Gram-negative bacterial infection
including foodborne pathogens.
-
Citations
Citations to this article as recorded by  - Isolation and characterization of Salmonella enteritidis bacteriophage Salmp-p7 isolated from slaughterhouse effluent and its application in food
Mengge Chen, Tong Yu, Xiangyu Cao, Jiaqi Pu, Deshu Wang, Hongkuan Deng
Archives of Microbiology.2025;[Epub] CrossRef - Can natural preservatives serve as a new line of protective technology against bacterial pathogens in meat and meat products?
Changyong Cheng, Lingli Jiang, Xiaoliang Li, Houhui Song, Weihuan Fang
Food Quality and Safety.2024;[Epub] CrossRef - Bacteriophage as a novel therapeutic approach for killing multidrug-resistant Escherichia coli ST131 clone
Md Shamsuzzaman, Shukho Kim, Jungmin Kim
Frontiers in Microbiology.2024;[Epub] CrossRef - Characterization of two virulent Salmonella phages and transient application in egg, meat and lettuce safety
XiaoWen Sun, Fan Xue, Cong Cong, Bilal Murtaza, LiLi Wang, XiaoYu Li, ShuYing Li, YongPing Xu
Food Research International.2024; 190: 114607. CrossRef - Advanced strategies to overcome the challenges of bacteriophage-based antimicrobial treatments in food and agricultural systems
Shanshan Liu, Siew-Young Quek, Kang Huang
Critical Reviews in Food Science and Nutrition.2024; 64(33): 12574. CrossRef - Review of phage display: A jack-of-all-trades and master of most biomolecule display
Brenda Pei Chui Song, Angela Chiew Wen Ch'ng, Theam Soon Lim
International Journal of Biological Macromolecules.2024; 256: 128455. CrossRef - Application of the lytic bacteriophage Rostam to control Salmonella enteritidis in eggs
Rahim Azari, Mohammad Hashem Yousefi, Zohreh Taghipour, Jeroen Wagemans, Rob Lavigne, Saeid Hosseinzadeh, Seyed Mohammad Mazloomi, Marta Vallino, Sepideh Khalatbari-Limaki, Enayat Berizi
International Journal of Food Microbiology.2023; 389: 110097. CrossRef - Isolation and genomic characterization of Vmp-1 using Vibrio mimicus as the host: A novel virulent bacteriophage capable of cross-species lysis against three Vibrio spp.
Bin Yang, Yang Wang, Lu Gao, Sheng-qi Rao, Wen-yuan Zhou, Zhen-quan Yang, Xin-an Jiao, Benjamin Kumah Mintah, Mokhtar Dabbour
Microbial Pathogenesis.2023; 174: 105948. CrossRef - A Review on the Antimicrobial Effect of Honey on Salmonella and Listeria monocytogenes: Recent Studies
Fatih Ramazan İSTANBULLUGİL, Nuri TAŞ, Ulaş ACARÖZ, Damla ARSLAN-ACAROZ, Ömer ÇAKMAK, Sezen EVRENKAYA, Zeki GÜRLER
Manas Journal of Agriculture Veterinary and Life Sciences.2023; 13(2): 210. CrossRef - Characterization of a Diverse Collection of Salmonella Phages Isolated from Tennessee Wastewater
Daniel W. Bryan, Lauren K. Hudson, Jia Wang, Thomas G. Denes
PHAGE.2023; 4(2): 90. CrossRef - Newly Isolated Virulent Salmophages for Biocontrol of Multidrug-Resistant Salmonella in Ready-to-Eat Plant-Based Food
Michał Wójcicki, Olga Świder, Paulina Średnicka, Dziyana Shymialevich, Tomasz Ilczuk, Łukasz Koperski, Hanna Cieślak, Barbara Sokołowska, Edyta Juszczuk-Kubiak
International Journal of Molecular Sciences.2023; 24(12): 10134. CrossRef - Prevalence of Indigenous Antibiotic-Resistant Salmonella Isolates and Their Application to Explore a Lytic Phage vB_SalS_KFSSM with an Intra-Broad Specificity
Jaein Choe, Su-Hyeon Kim, Ji Min Han, Jong-Hoon Kim, Mi-Sun Kwak, Do-Won Jeong, Mi-Kyung Park
Journal of Microbiology.2023; 61(12): 1063. CrossRef - Statistical optimization of a podoviral anti-MRSA phage CCASU-L10 generated from an under sampled repository: Chicken rinse
Israa M. Abd-Allah, Ghadir S. El-Housseiny, Mohamed H. Al-Agamy, Hesham H. Radwan, Khaled M. Aboshanab, Nadia A. Hassouna
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef - Characterization and Genome Study of a Newly Isolated Temperate Phage Belonging to a New Genus Targeting Alicyclobacillus acidoterrestris
Dziyana Shymialevich, Michał Wójcicki, Olga Świder, Paulina Średnicka, Barbara Sokołowska
Genes.2023; 14(6): 1303. CrossRef - An Anti-MRSA Phage From Raw Fish Rinse: Stability Evaluation and Production Optimization
Israa M. Abd-Allah, Ghadir S. El-Housseiny, Mohammad Y. Alshahrani, Samar S. El-Masry, Khaled M. Aboshanab, Nadia A. Hassouna
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef - Anti-Salmonella polyvinyl alcohol coating containing a virulent phage PBSE191 and its application on chicken eggshell
Sangbin Kim, Yoonjee Chang
Food Research International.2022; 162: 111971. CrossRef - Applications of bacteriophages against intracellular bacteria
Paulina Śliwka, Marta Ochocka, Aneta Skaradzińska
Critical Reviews in Microbiology.2022; 48(2): 222. CrossRef - In Vitro and In Vivo Gastrointestinal Survival of Non-Encapsulated and Microencapsulated Salmonella Bacteriophages: Implications for Bacteriophage Therapy in Poultry
Laura Lorenzo-Rebenaque, Danish J. Malik, Pablo Catalá-Gregori, Clara Marin, Sandra Sevilla-Navarro
Pharmaceuticals.2021; 14(5): 434. CrossRef - How Broad Is Enough: The Host Range of Bacteriophages and Its Impact on the Agri-Food Sector
Karen Fong, Catherine W.Y. Wong, Siyun Wang, Pascal Delaquis
PHAGE.2021; 2(2): 83. CrossRef - Characterization and Application of a Lytic Phage D10 against Multidrug-Resistant Salmonella
Zhiwei Li, Wanning Li, Wenjuan Ma, Yifeng Ding, Yu Zhang, Qile Yang, Jia Wang, Xiaohong Wang
Viruses.2021; 13(8): 1626. CrossRef - Antimicrobial activity of LysSS, a novel phage endolysin, against Acinetobacter baumannii and Pseudomonas aeruginosa
Shukho Kim, Da-Won Lee, Jong-Sook Jin, Jungmin Kim
Journal of Global Antimicrobial Resistance.2020; 22: 32. CrossRef - Characterization of the narrow-spectrum bacteriophage LSE7621 towards Salmonella Enteritidis and its biocontrol potential on lettuce and tofu
Aiping Liu, Yilin Liu, Lin Peng, Xingzhe Cai, Li Shen, Maoping Duan, Yu Ning, Shuliang Liu, Chunyan Li, Yuntao Liu, Hong Chen, Wenjuan Wu, Xiaohong Wang, Bin Hu, Cheng Li
LWT.2020; 118: 108791. CrossRef - State of the Art in the Culture of the Human Microbiota: New Interests and Strategies
Maryam Tidjani Alou, Sabrina Naud, Saber Khelaifia, Marion Bonnet, Jean-Christophe Lagier, Didier Raoult
Clinical Microbiology Reviews.2020;[Epub] CrossRef - Lytic KFS-SE2 phage as a novel bio-receptor for Salmonella Enteritidis detection
In Young Choi, Cheonghoon Lee, Won Keun Song, Sung Jae Jang, Mi-Kyung Park
Journal of Microbiology.2019; 57(2): 170. CrossRef
- Inverse PCR for subtyping of Acinetobacter baumannii carrying ISAba1
-
Shukho Kim , Yun-Ju Park , Jungmin Kim
-
J. Microbiol. 2016;54(5):376-380. Published online April 20, 2016
-
DOI: https://doi.org/10.1007/s12275-016-6038-3
-
-
72
View
-
0
Download
-
3
Crossref
-
Abstract
-
Acinetobacter baumannii has been prevalent in nosocomial
infections, often causing outbreaks in intensive care units.
ISAba1 is an insertion sequence that has been identified only
in A. baumannii and its copy number varies among strains.
It has been reported that ISAba1 provides a promoter for
blaOXA-51-like, blaOXA-23-like, and blaampC, which are associated
with the resistance of A. baumannii to carbapenems and cephalosporins.
The main purpose of this study was to develop
a novel inverse PCR method capable of typing A. baumannii
strains. The method involves three major steps: cutting of genomic
DNA with a restriction enzyme, ligation, and PCR.
In the first step, bacterial genomic DNA was digested with
DpnI. In the second step, the digested genomic DNAs were
ligated to form intramolecular circular DNAs. In the last step,
the ligated circular DNAs were amplified by PCR with primers
specific for ISAba1 and the amplified PCR products
were electrophoresed. Twenty-two clinical isolates of A. baumannii
were used for the evaluation of the inverse PCR (iPCR)
typing method. Dendrogram analysis revealed two major clusters,
similar to pulsed-field gel electrophoresis (PFGE) results.
Three ISAba1-associated genes – blaampC, blaOXA-66-like, and
csuD – were amplified and detected in the clinical isolates.
This novel iPCR typing method is comparable to PFGE in its
ability to discriminate A. baumannii strains, and is a promising
molecular epidemiological tool for investigating A.
baumannii carrying ISAba1.
-
Citations
Citations to this article as recorded by - DNA sonication inverse PCR for genome scale analysis of uncharacterized flanking sequences
David E. Alquezar‐Planas, Ulrike Löber, Pin Cui, Claudia Quedenau, Wei Chen, Alex D. Greenwood, Susan Johnston
Methods in Ecology and Evolution.2021; 12(1): 182. CrossRef -
Update on the Epidemiological Typing Methods for
Acinetobacter Baumannii
Rayane Rafei, Marwan Osman, Fouad Dabboussi, Monzer Hamze
Future Microbiology.2019; 14(12): 1065. CrossRef - Identification and characterization of a novel cold-tolerant extracellular protease from Planococcus sp. CGMCC 8088
Kun Chen, Qingshan Mo, Huan Liu, Feiyan Yuan, Haonan Chai, Fuping Lu, Huitu Zhang
Extremophiles.2018; 22(3): 473. CrossRef
- Antibacterial Efficacy of Lytic Pseudomonas Bacteriophage in Normal and Neutropenic Mice Models
-
Birendra R. Tiwari , Shukho Kim , Marzia Rahman , Jungmin Kim
-
J. Microbiol. 2011;49(6):994-999. Published online December 28, 2011
-
DOI: https://doi.org/10.1007/s12275-011-1512-4
-
-
43
View
-
0
Download
-
61
Scopus
-
Abstract
-
Recently, lytic bacteriophages (phages) have been focused on treating bacterial infectious diseases. We investigated the protective efficacy of a novel Pseudomonas aeruginosa phage, PA1Ø, in normal and neutropenic mice. A lethal dose of P. aeruginosa PAO1 was administered via the intraperitoneal route and a single
dose of PA1Ø with different multiplicities of infection (MOI) was treated into infected mice. Immunocompetent mice infected with P. aeruginosa PAO1 were successfully protected by PA1Ø of 1 MOI, 10 MOI or 100 MOI with 80% to 100% survival rate. No viable bacteria were found in organ samples after 48 h of the phage treatment. Phage clearing patterns were different in the presence or absence of host bacteria but PA1Ø disappeared from all organs after 72 h except spleen in the presence of host bacteria. On the contrary, PA1Ø treatment could not protect neutropenic mice infected with P. aeruginosa PAO1 even though could extend their lives for a short time. In in vitro phage-neutrophil bactericidal test, a stronger bactericidal effect was observed in phage-neutrophil co-treatment than in phage single treatment without neutrophils, suggesting phage-neutrophil co-work is essential for the efficient killing of bacteria in the mouse model. In conclusion, PA1Ø can be possibly utilized in future phage therapy endeavors since it exhibited strong protective effects against virulent P. aeruginosa infection.
- Interaction of Acinetobacter baumannii 19606 and 1656-2 with Acanthamoeba castellanii
-
Migma Dorji Tamang , Shukho Kim , Sung-Min Kim , Hyun-Hee Kong , Jungmin Kim
-
J. Microbiol. 2011;49(5):841-846. Published online November 9, 2011
-
DOI: https://doi.org/10.1007/s12275-011-1063-8
-
-
44
View
-
0
Download
-
9
Scopus
-
Abstract
-
Acinetobacter baumannii is virtually avirulent for healthy people but maintains a high virulence among critically ill patients or immuno-compromised individuals. The ability of A. baumannii to adhere to cells and persist on surfaces as biofilms could be central to its pathogenicity. In the present study, we compared the virulence of the A. baumannii 1656-2 clinical strain, which is able to form a thick biofilm, with the virulence of the A. baumannii type strain (ATCC 19606T). Acanthamoeba castellanii, a single-celled organism, was used as the host model system to study the virulence of A. baumannii. Compared to A. baumannii ATCC 19606T, A. baumannii 1656-2 exhibited a higher ability to adhere and invade A. castellanii cells and had a higher killing rate of A. castellanii cells. Furthermore, co-incubation of the amoeba cells and the cell-free supernatant of A. baumannii resulted in the cell death of the amoebae. Heat inactivation or proteinase K treatment of the supernatant did not eliminate its cytotoxicity, suggesting heat stable non-protein factors are responsible for its cytotoxicity to A. castellanii cells. In conclusion, this study for the first time has revealed the capacity of the A. baumannii strain and/or its metabolic products to induce cytotoxicity in A. castellanii cells.
- Providencia Isolates Carrying blaPER-1 and blaVIM-2 Genes: Biofilm-Forming Capacity and Biofilm Inhibitory Concentrations for Carbapenem Antibiotics
-
Jungmin Kim , Shukho Kim , Hee Woo Lee , Sung Min Kim , Sung Yong Seol
-
J. Microbiol. 2011;49(3):512-515. Published online June 30, 2011
-
DOI: https://doi.org/10.1007/s12275-011-1221-z
-
-
Abstract
-
Multidrug-resistant clinical isolates of Providencia carrying blaPER-1 and blaVIM-2 were evaluated for the abilities to form biofilm and high biofilm forming capacity was demonstrated in them. Minimum biofilm inhibitory concentrations (MBICs), minimum biofilm eradication concentrations (MBECs), and minimum inhibitory concentrations (MICs) for imipenem and meropenem were also determined. In all tested strains, the MBICs were higher than the MICs for both drugs. Interestingly, the MBICs and the MBEC50 for meropenem were lower than those for imipenem in the isolates producing high amounts of biofilm, suggesting that meropenem is superior to imipenem in the growth inhibition and eradication of biofilm forming Providencia strains.
- A Simple Colorimetric Method for Testing Antimicrobial Susceptibility of Biofilmed Bacteria
-
Shukho Kim , Mi Jin Kim , Hee Young Kang , Sung Yong Seol , Dong Taek Cho , Jungmin Kim
-
J. Microbiol. 2010;48(5):709-711. Published online November 3, 2010
-
DOI: https://doi.org/10.1007/s12275-010-0299-z
-
-
43
View
-
0
Download
-
21
Crossref
-
Abstract
-
This study introduces a simple colorimetric method which can measure the antimicrobial susceptibility of bacteria in biofilms using trimethyl tetrazolium chloride (TTC) as an indicator of viable bacteria. The new method was utilized for the evaluation of antibiotic susceptibility of Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus biofilms.
-
Citations
Citations to this article as recorded by - Antibiofilm and Antimicrobial Potentials of Novel Synthesized Sulfur Camphor Derivatives
Anna Duda-Madej, Szymon Viscardi, Katarzyna Pacyga, Robert Kupczyński, Wanda Mączka, Małgorzata Grabarczyk, Paweł Pacyga, Ewa Topola, Michał Ostrówka, Jacek Bania, Antoni Szumny, Katarzyna Wińska
International Journal of Molecular Sciences.2024; 25(20): 10895. CrossRef - Bioadhesives with Antimicrobial Properties
Mustafa Nakipoglu, Ayşen Tezcaner, Christopher H. Contag, Nasim Annabi, Nureddin Ashammakhi
Advanced Materials.2023;[Epub] CrossRef - The impact of biosynthesized ZnO nanoparticles from Olea europaea (Common Olive) on Pseudomonas aeruginosa growth and biofilm formation
Hafez Al-Momani, Dua’a Al Balawi, Saja Hamed, Borhan Aldeen Albiss, Muna Almasri, Hadeel AlGhawrie, Lujain Ibrahim, Hadeel Al Balawi, Sameer Al Haj Mahmoud, Jeffrey Pearson, Christopher Ward
Scientific Reports.2023;[Epub] CrossRef - Does Secondary Plant Metabolite Ursolic Acid Exhibit Antibacterial Activity against Uropathogenic Escherichia coli Living in Single- and Multispecies Biofilms?
Zuzanna Sycz, Dorota Wojnicz, Dorota Tichaczek-Goska
Pharmaceutics.2022; 14(8): 1691. CrossRef - Are Uropathogenic Bacteria Living in Multispecies Biofilm Susceptible to Active Plant Ingredient—Asiatic Acid?
Zuzanna Sycz, Dorota Tichaczek-Goska, Anna Jezierska-Domaradzka, Dorota Wojnicz
Biomolecules.2021; 11(12): 1754. CrossRef - Antibacterial activities of and biofilm removal by Ablysin, an endogenous lysozyme-like protein originated from Acinetobacter baumannii 1656-2
Shukho Kim, Jong-Sook Jin, Da-Won Lee, Jungmin Kim
Journal of Global Antimicrobial Resistance.2020; 23: 297. CrossRef - Antibacterial action of nitric oxide-releasing hyperbranched polymers against ex vivo dental biofilms
Lei Yang, Flavia Teles, Weida Gong, Shawn A. Dua, Lynn Martin, Mark H. Schoenfisch
Dental Materials.2020; 36(5): 635. CrossRef - Susceptibility to biofilm formation on 3D-printed titanium fixation plates used in the mandible: a preliminary study
Lukasz Palka, Justyna Mazurek-Popczyk, Katarzyna Arkusz, Katarzyna Baldy-Chudzik
Journal of Oral Microbiology.2020; 12(1): 1838164. CrossRef - Anti-biofilm effects and characterisation of the hydrogen peroxide activity of a range of Western Australian honeys compared to Manuka and multifloral honeys
Azhar Sindi, Moses Van Bawi Chawn, Magda Escorcia Hernandez, Kathryn Green, Md Khairul Islam, Cornelia Locher, Katherine Hammer
Scientific Reports.2019;[Epub] CrossRef - Functional assessment of microbial superoxide dismutase isozymes suggests a differential role for each isozyme
Hastyar Najmuldeen, Rashed Alghamdi, Fayez Alghofaili, Hasan Yesilkaya
Free Radical Biology and Medicine.2019; 134: 215. CrossRef - Polymicrobial Biofilm Inhibition Effects of Acetate‐Buffered Chitosan Sponge Delivery Device
Jessica Amber Jennings, Karen E. Beenken, Ashley C. Parker, James Keaton Smith, Harry S. Courtney, Mark S. Smeltzer, Warren O. Haggard
Macromolecular Bioscience.2016; 16(4): 591. CrossRef - Chronic Wound Biofilms: Pathogenesis and Potential Therapies
Allie Clinton, Tammy Carter
Laboratory Medicine.2015; 46(4): 277. CrossRef - Isolation and characterization of an Enterococcus faecalis bacteriophage
Hee-Young Kang, Shukho Kim, Jungmin Kim
The Korean Journal of Microbiology.2015; 51(3): 194. CrossRef - Effects of Azithromycin, Metronidazole, Amoxicillin, and Metronidazole plus Amoxicillin on anIn VitroPolymicrobial Subgingival Biofilm Model
Geisla M. S. Soares, Flavia Teles, Jacqueline R. Starr, Magda Feres, Michele Patel, Lynn Martin, Ricardo Teles
Antimicrobial Agents and Chemotherapy.2015; 59(5): 2791. CrossRef - Pyocyanin Stimulates Quorum Sensing-Mediated Tolerance to Oxidative Stress and Increases Persister Cell Populations in Acinetobacter baumannii
Nidhi Bhargava, Prince Sharma, Neena Capalash, B. A. McCormick
Infection and Immunity.2014; 82(8): 3417. CrossRef - Optimization of tetrazolium salt assay for Pseudomonas aeruginosa biofilm using microtiter plate method
Parastoo Sabaeifard, Ahya Abdi-Ali, Mohammad Reza Soudi, Rasoul Dinarvand
Journal of Microbiological Methods.2014; 105: 134. CrossRef - Study of 2,3,5-Triphenyltetrazolium Chloride for Detection of Pathogenic Microorganisms
Jung Wook Kang, Jun Tae Bae, Jae Young Yeon, Young Ho Kim, Jin Hwa Kim, Geun Soo Lee, Hyeong Bae Pyo
Journal of the Society of Cosmetic Scientists of Korea.2014; 40(3): 307. CrossRef - Pseudomonas aeruginosa Bacteriophage PA1Ø Requires Type IV Pili for Infection and Shows Broad Bactericidal and Biofilm Removal Activities
Shukho Kim, Marzia Rahman, Sung Yong Seol, Sang Sun Yoon, Jungmin Kim
Applied and Environmental Microbiology.2012; 78(17): 6380. CrossRef - The inhibitory effect of phloretin on the formation of Escherichia coli O157:H7 biofilm in a microfluidic system
Aeri Park, Heon-Ho Jeong, Jintae Lee, Chang-Soo Lee
BioChip Journal.2012; 6(3): 299. CrossRef - Characterization of inducedStaphylococcus aureusbacteriophage SAP-26 and its anti-biofilm activity with rifampicin
Marzia Rahman, Shukho Kim, Sung Min Kim, Sung Yong Seol, Jungmin Kim
Biofouling.2011; 27(10): 1087. CrossRef - Providencia isolates carrying bla PER-1 and bla VIM-2 genes: Biofilm-forming capacity and biofilm inhibitory concentrations for carbapenem antibiotics
Jungmin Kim, Shukho Kim, Hee Woo Lee, Sung Min Kim, Sung Yong Seol
The Journal of Microbiology.2011; 49(3): 512. CrossRef
- Phage Types and Pulsed-Field Gel Electrophoresis Patterns of Salmonella enterica serovar Enteritidis Isolated from Humans and Chickens
-
Sung Hun Kim , Shukho Kim , Sung Guen Chun , Mi-Sun Park , Jeong Hyun Park , Bok-Kwon Lee
-
J. Microbiol. 2008;46(2):209-213. Published online June 11, 2008
-
DOI: https://doi.org/10.1007/s12275-007-0197-1
-
-
55
View
-
0
Download
-
14
Scopus
-
Abstract
-
We analyzed 66 Salmonella Enteritidis isolates in 2002. Thirty isolates were obtained from human patients with diarrhea, and 36 were obtained from chickens. A total of ten phage types (PT) were identified in the human and chicken isolates. PT1 and PT21 were the predominant PTs in both the human (20% and 13%) and chicken (17% and 47%) isolates. Twelve pulsotypes were generated by PFGE and divided into two major groups. Most of the PFGE types were categorized into cluster group 1. Eighteen chicken isolates in cluster group 1 showed high-level genetic association (>95%) with 22 other human isolates. Additionally, six chicken
isolates from cluster group 2 showed fairly high-level genetic association (>95%) with the other seven human isolates. The highest levels of genetic association in humans and chickens were seen with A5-PT21 (11 isolates), A2-PT1 (7 isolates), and B1-PT4 (6 isolates). The Pulsed-Field Gel Electrophoresis (PFGE) and phage typing provided conclusive evidence that human Salmonella infections are attributable to the consumption of contaminated chicken.
- Genomic Relationship of Salmonella enterica Serovar Typhimurium DT104 Isolates from Korea and the United States
-
Shukho Kim , Sung Guen Chun , Ok Young Lim , Mi Sun Park , Yeon Ho Kang , Yong Ho Park , Bok Kwon Lee
-
J. Microbiol. 2004;42(1):14-19.
-
DOI: https://doi.org/2007 [pii]
-
-
Abstract
-
Salmonella enterica serovar Typhimurium DT104 (Salmonella Typhimurium DT104 or DT104) hasbeen emerging as a common pathogen for human in Korea since 1997. In order to compare the genomic relationship and to search for the dominant strains in Korea, we conducted pulsed-field gel electrophoresis (PFGE) and IS200 fingerprinting of 25 epidemiological unrelated isolates from human and animals from Korea and cattle from America. Two Salmonella Typhimurium DT104 isolates from human in Korea and all 8 isolates from American cattle had indistinguishable patterns from the PFGE and IS200 fingerprinting but multidrug-resistant Salmonella Typhimurium isolates, including DT104, from Korean animals had diverse genetic patterns. The data suggest that a dominant DT104 strain might have circulated between Korean and American cattle and that it had a high level of clonality.
|
MSK
