- Characterization of transcriptional activities at a divergent promoter of the type VI secretion system in enterohemorrhagic Escherichia coli O157:H7
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Se Kye Kim , Jun Bong Lee , Jang Won Yoon
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J. Microbiol. 2022;60(9):928-934. Published online August 19, 2022
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DOI: https://doi.org/10.1007/s12275-022-2109-9
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 Abstract
 PDF
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The type VI secretion system (T6SS) is a novel secretion system
found in many Gram-negative bacteria that plays a role
in bacterial competition, virulence, and host immune evasion.
The enterohemorrhagic Escherichia coli (EHEC) O157:H7
strain EDL933 has a single functional T6SS gene cluster. In
this study, we attempted to characterize the transcriptional
pattern of the T6SS effector gene Z0264 in EDL933. Transcriptional
analyses showed that Z0264 and other T6SS genes
were transcribed in vitro in a growth-phase-dependent manner,
but Z0264 was not secreted in the rich medium. Using
adapter- and radioactivity-free transcription start site analysis,
we identified an overlapping divergent promoter between
Z0264 and Z0265. A β-galactosidase assay with truncated promoter
regions showed that the divergent promoter is functional.
In addition, we demonstrated the role of H-NS as a
repressor in the transcription of Z0264. Notably, the cDNA
PCR assay showed that the mRNA transcript from the Z0264
promoter did not include the entire main T6SS cluster, suggesting
segmented gene expression by multiple promoters in
the T6SS cluster. In conclusion, we identified a divergent promoter
for Z0264 located in the T6SS cluster of EDL933 and
characterized its in vitro transcriptional activity during growth.
Our findings provide insights and a preliminary understanding
of the regulatory mechanisms underlying T6SS transcription.
- MINIREVIEW] Antisense peptide nucleic acids as a potential anti-infective agent
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Hyung Tae Lee , Se Kye Kim , Jang Won Yoon
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J. Microbiol. 2019;57(6):423-430. Published online May 27, 2019
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DOI: https://doi.org/10.1007/s12275-019-8635-4
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Abstract
PDF
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Antibiotics have long been used for anti-infective control of
bacterial infections, growth promotion in husbandry, and
prophylactic protection against plant pathogens. However,
their inappropriate use results in the emergence and spread
of multiple drug resistance (MDR) especially among various
bacterial populations, which limits further administration
of conventional antibiotics. Therefore, the demand for novel
anti-infective approaches against MDR diseases becomes
increasing in recent years. The peptide nucleic acid (PNA)-
based technology has been proposed as one of novel antiinfective
and/or therapeutic strategies. By definition, PNA
is an artificially synthesized nucleic acid mimic structurally
similar to DNA or RNA in nature and linked one another via
an unnatural pseudo-peptide backbone, rendering to its stability
in diverse host conditions. It can bind DNA or RNA
strands complimentarily with high affinity and sequence specificity,
which induces the target-specific gene silencing by
inhibiting transcription and/or translation. Based on these
unique properties, PNA has been widely applied for molecular
diagnosis as well as considered as a potential anti-infective
agent. In this review, we discuss the general features
of PNAs and their application to various bacterial pathogens
as new anti-infective or antimicrobial agents.
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Citations
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