- Characterization of components of a reducing system for SoxR in the cytoplasmic membrane of Escherichia coli
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Kang-Lok Lee , Kyung-Chang Lee , Joon-Hee Lee , Jung-Hye Roe
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J. Microbiol. 2022;60(4):387-394. Published online March 28, 2022
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DOI: https://doi.org/10.1007/s12275-022-1667-1
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82
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 Abstract
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A reducing system of SoxR, a regulator of redox-active molecules,
was identified as rsxABCDGE gene products and RseC
in Escherichia coli through genetic studies. We found that
ApbE was an additional component of the reducer system.
Bacterial two hybrid analysis revealed that these proteins indeed
had multiple interactions among themselves. RseC and
RsxB formed the core of the complex, interacting with more
than five other components. RsxC, the only cytoplasmic component
of the system, interacted with SoxR. It might be linked
with the rest of the complex via RsxB. Membrane fractions
containing the wild type complex but not the mutant complex
reduced purified SoxR using NADH as an electron source.
These results suggest that Rsx genes, RseC, and ApbE can
form a complex using NAD(P)H to reduce SoxR.
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Citations
Citations to this article as recorded by  - AcrAB-TolC efflux pump overexpression and tet(A) gene mutation increase tigecycline resistance in Klebsiella pneumoniae
Zhaoxin Xia, Jing zhou, Nana Gao, Ge Li, Runde Liu, Guoping Lu, Jilu Shen
World Journal of Microbiology and Biotechnology.2024;[Epub] CrossRef - The Na+-translocating NADH:quinone oxidoreductase (Na+-NQR): Physiological role, structure and function of a redox-driven, molecular machine
Julia Steuber, Günter Fritz
Biochimica et Biophysica Acta (BBA) - Bioenergetics.2024; 1865(4): 149485. CrossRef - Functional analysis of bacterial genes accidentally packaged in rhizospheric phageome of the wild plant species Abutilon fruticosum
Ruba Abdulrahman Ashy
Saudi Journal of Biological Sciences.2023; 30(10): 103789. CrossRef
- Regulation of iron homeostasis by peroxide-sensitive CatR, a Fur-family regulator in Streptomyces coelicolor
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Yeonbum Kim , Jung-Hye Roe , Joo-Hong Park , Yong-Joon Cho , Kang-Lok Lee
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J. Microbiol. 2021;59(12):1083-1091. Published online December 4, 2021
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DOI: https://doi.org/10.1007/s12275-021-1457-1
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83
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5
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5
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Abstract
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CatR, a peroxide-sensing transcriptional repressor of Fur
family, can de-repress the transcription of the catA gene encoding
catalase upon peroxide stress in Streptomyces coelicolor.
Since CatR-regulated genes other than catA and its own
gene catR have not been identified in detail, the understanding
of the role of CatR regulon is very limited. In this study,
we performed transcriptomic analysis to identify genes influenced
by both atR mutation and hydrogen peroxide treatment.
Through ChIP-qPCR and other analyses, a new consensus
sequence was found in CatR-responsive promoter region
of catR gene and catA operon for direct regulation. In
addition, vtlA (SCO2027) and SCO4983 were identified as new
members of the CatR regulon. Expression levels of iron uptake
genes were reduced by hydrogen peroxide and a DmdR1 binding
sequence was identified in promoters of these genes. The
increase in free iron by hydrogen peroxide was thought to
suppress the iron import system by DmdR1. A putative exporter
protein VtlA regulated by CatR appeared to reduce intracellular
iron to prevent oxidative stress. The name vtlA
(VIT1-like transporter) was proposed for iron homeostasis
related gene SCO2027.
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Citations
Citations to this article as recorded by - Structure, Function, and Biosynthesis of Siderophores Produced by Streptomyces Species
Mingxuan Wang, Honglin Li
Journal of Agricultural and Food Chemistry.2025; 73(8): 4425. CrossRef - Autonomous Defense Based on Biogenic Nanoparticle Formation in Daunomycin-Producing Streptomyces
Karel Beneš, Vladislav Čurn, Baveesh Pudhuvai, Jaroslav Motis, Zuzana Michalcová, Andrea Bohatá, Jana Lencová, Jan Bárta, Michael Rost, Andreas Vilcinskas, Vladimír Maťha
Microorganisms.2025; 13(1): 107. CrossRef -
Functional versatility of Zur in metal homeostasis, motility, biofilm formation, and stress resistance in
Yersinia pseudotuberculosis
Yanchao Gu, Yongde Liu, Wei Mao, Ying Peng, Xiaoru Han, Han Jin, Jingling Xu, Liyang Chang, Yixin Hou, Xihui Shen, Xingyu Liu, Yantao Yang, G. Marcela Rodriguez
Microbiology Spectrum.2024;[Epub] CrossRef -
Multi-integrated approach for unraveling small open reading frames potentially associated with secondary metabolism in
Streptomyces
Si-Min Fan, Ze-Qi Li, Shi-Zhe Zhang, Liang-Yu Chen, Xi-Ying Wei, Jian Liang, Xin-Qing Zhao, Chun Su, Xiao-Hua Zhang
mSystems.2023;[Epub] CrossRef - The regulatory role of Fur-encoding SCLAV_3199 in iron homeostasis in Streptomyces clavuligerus
Büşra Abanoz-Seçgin, Çiğdem Otur, Sezer Okay, Aslıhan Kurt-Kızıldoğan
Gene.2023; 878: 147594. CrossRef
- Non-mitochondrial aconitase regulates the expression of iron-uptake genes by controlling the RNA turnover process in fission yeast
-
Soo-Yeon Cho , Soo-Jin Jung , Kyoung-Dong Kim , Jung-Hye Roe
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J. Microbiol. 2021;59(12):1075-1082. Published online October 26, 2021
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DOI: https://doi.org/10.1007/s12275-021-1438-4
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75
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5
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5
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Abstract
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Aconitase, a highly conserved protein across all domains of
life, functions in converting citrate to isocitrate in the tricarboxylic
acid cycle. Cytosolic aconitase is also known to act as
an iron regulatory protein in mammals, binding to the RNA
hairpin structures known as iron-responsive elements within
the untranslated regions of specific RNAs. Aconitase-2 (Aco2)
in fission yeast is a fusion protein consisting of an aconitase
and a mitochondrial ribosomal protein, bL21, residing not
only in mitochondria but also in cytosol and the nucleus. To
investigate the role of Aco2 in the nucleus and cytoplasm of
fission yeast, we analyzed the transcriptome of aco2ΔN mutant
that is deleted of nuclear localization signal (NLS). RNA
sequencing revealed that the aco2ΔN mutation caused increase
in mRNAs encoding iron uptake transporters, such as
Str1, Str3, and Shu1. The half-lives of mRNAs for these genes
were found to be significantly longer in the aco2ΔN mutant
than the wild-type strain, suggesting the role of Aco2 in mRNA
turnover. The three conserved cysteines required for the catalytic
activity of aconitase were not necessary for this role.
The UV cross-linking RNA immunoprecipitation analysis
revealed that Aco2 directly bound to the mRNAs of iron uptake
transporters. Aco2-mediated degradation of iron-uptake
mRNAs appears to utilize exoribonuclease pathway that involves
Rrp6 as evidenced by genetic interactions. These results
reveal a novel role of non-mitochondrial aconitase protein
in the mRNA turnover in fission yeast to fine-tune iron
homeostasis, independent of regulation by transcriptional
repressor Fep1.
-
Citations
Citations to this article as recorded by - Iron-mediated post-transcriptional regulation in Toxoplasma gondii
Megan A. Sloan, Adam Scott, Dana Aghabi, Lucia Mrvova, Clare R. Harding, Dominique Soldati-Favre
PLOS Pathogens.2025; 21(2): e1012857. CrossRef - The Key Enzymes of Carbon Metabolism and the Glutathione Antioxidant System Protect Yarrowia lipolytica Yeast Against pH-Induced Stress
Tatyana I. Rakhmanova, Natalia N. Gessler, Elena P. Isakova, Olga I. Klein, Yulia I. Deryabina, Tatyana N. Popova
Journal of Fungi.2024; 10(11): 747. CrossRef - The intricate link between iron, mitochondria and azoles in Candida species
Wouter Van Genechten, Rudy Vergauwen, Patrick Van Dijck
The FEBS Journal.2024; 291(16): 3568. CrossRef - Non-Mitochondrial Aconitase-2 Mediates the Transcription of Nuclear-Encoded Electron Transport Chain Genes in Fission Yeast
Ho-Jung Kim, Soo-Yeon Cho, Soo-Jin Jung, Yong-Jun Cho, Jung-Hye Roe, Kyoung-Dong Kim
Journal of Microbiology.2024; 62(8): 639. CrossRef - Kinetic and Regulatory Properties of Yarrowia lipolytica Aconitate Hydratase as a Model-Indicator of Cell Redox State under pH Stress
Tatyana I. Rakhmanova, Varvara Yu. Sekova, Natalya N. Gessler, Elena P. Isakova, Yulia I. Deryabina, Tatyana N. Popova, Yevgeniya I. Shurubor, Boris F. Krasnikov
International Journal of Molecular Sciences.2023; 24(8): 7670. CrossRef
- Growth and differentiation properties of pikromycin-producing Streptomyces venezuelae ATCC15439
-
Ji-Eun Kim , Joon-Sun Choi , Jung-Hye Roe
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J. Microbiol. 2019;57(5):388-395. Published online February 5, 2019
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DOI: https://doi.org/10.1007/s12275-019-8539-3
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69
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6
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Abstract
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Streptomycetes naturally produce a variety of secondary
metabolites, in the process of physiological differentiation.
Streptomyces venezuelae differentiates into spores in liquid
media, serving as a good model system for differentiation and
a host for exogenous gene expression. Here, we report the
growth and differentiation properties of S. venezuelae ATCC-
15439 in liquid medium, which produces pikromycin, along
with genome-wide gene expression profile. Comparison of
growth properties on two media (SPA, MYM) revealed that
the stationary phase cell viability rapidly decreased in SPA.
Submerged spores showed partial resistance to lysozyme and
heat, similar to what has been observed for better-characterized
S. venezuelae ATCC10712, a chloramphenicol producer.
TEM revealed that the differentiated cells in the submerged
culture showed larger cell size, thinner cell wall than
the aerial spores. We analyzed transcriptome profiles of cells
grown in liquid MYM at various growth phases. During
transition and/or stationary phases, many differentiationrelated
genes were well expressed as judged by RNA level,
except some genes forming hydrophobic coats in aerial mycelium.
Since submerged spores showed thin cell wall and
partial resistance to stresses, we examined cellular expression
of MreB protein, an actin-like protein known to be required
for spore wall synthesis in Streptomycetes. In contrast to
aerial spores where MreB was localized in septa and spore
cell wall, submerged spores showed no detectable signal.
Therefore, even though the mreB transcripts are abundant in
liquid medium, its protein level and/or its interaction with
spore wall synthetic complex appear impaired, causing thinner-
walled and less sturdy spores in liquid culture.
-
Citations
Citations to this article as recorded by - Biosynthesis of Arcyriaflavin F from Streptomyces venezuelae ATCC 10712
Hung‐En Lai, Agata Kennedy, Lewis Tanner, Emma A. Bartram, Soo Mei Chee, Paul S. Freemont, Simon J. Moore
ChemBioChem.2024;[Epub] CrossRef - Functional analysis of the whole CYPome and Fdxome of Streptomyces venezuelae ATCC 15439
Shuai Li, Zhong Li, Guoqiang Zhang, Vlada B. Urlacher, Li Ma, Shengying Li
Engineering Microbiology.2024; 4(4): 100166. CrossRef - Glucose-1-phosphate thymidylyltransferase promotes the production of 3-O-α-mycarosylerythronolide B in Streptomyces coelicolor
Hong Gao, Swen Langer, Tony Larson, Matthew A Gregory, Margaret C M Smith
Journal of Applied Microbiology.2024;[Epub] CrossRef - Comparative Metagenomics Reveals Microbial Signatures of Sugarcane Phyllosphere in Organic Management
Ahmad Nuruddin Khoiri, Supapon Cheevadhanarak, Jiraporn Jirakkakul, Sudarat Dulsawat, Peerada Prommeenate, Anuwat Tachaleat, Kanthida Kusonmano, Songsak Wattanachaisaereekul, Sawannee Sutheeworapong
Frontiers in Microbiology.2021;[Epub] CrossRef - Lysine acetylation of the housekeeping sigma factor enhances the activity of the RNA polymerase holoenzyme
Ji-Eun Kim, Joon-Sun Choi, Jong-Seo Kim, You-Hee Cho, Jung-Hye Roe
Nucleic Acids Research.2020; 48(5): 2401. CrossRef - Characterization of Actinomycetes Strains Isolated from the Intestinal Tract and Feces of the Larvae of the Longhorn Beetle Cerambyx welensii
Ramón I. Santamaría, Ana Martínez-Carrasco, Ricardo Sánchez de la Nieta, Luis M. Torres-Vila, Raúl Bonal, Jesús Martín, Rubén Tormo, Fernando Reyes, Olga Genilloud, Margarita Díaz
Microorganisms.2020; 8(12): 2013. CrossRef
- The Role and Regulation of Trx1, a Cytosolic Thioredoxin in Schizosaccharomyces pombe
-
Ji-Yoon Song , Jung-Hye Roe
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J. Microbiol. 2008;46(4):408-414. Published online August 31, 2008
-
DOI: https://doi.org/10.1007/s12275-008-0076-4
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71
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25
Crossref
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Abstract
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The genome of fission yeast Schizosaccharomyces pombe harbors two genes for thioredoxins, trx1+ and trx2+, which encode cytosolic and mitochondrial thioredoxins, respectively. The Δtrx1 mutant was found sensitive to diverse external stressors such as various oxidants, heat, and salt, whereas Δtrx2 mutant was not sensitive except to paraquat, a superoxide generator. Both Δtrx1 and Δtrx2 mutants were more resistant to diamide, a thiol-specific oxidant, than the wild type. The trx1+ gene expression was induced by H2O2 and menadione, being mediated through a stress-responsive transcription factor Pap1. In Δtrx1 cells, the basal expression of Pap1-regulated genes were elevated, suggesting a role for Trx1 as a reducer for oxidized (activated) Pap1. The Δtrx1 mutant exhibited cysteine auxotrophy, which can be overcome by adding sulfite. This suggests that Trx1 serves as a primary electron donor for 3’-phosphoadenosine-5’-phosphosulfate (PAPS) reductase and thus is an essential protein for sulfur assimilation in S. pombe. These results suggest that, in contrast to Trx2 whose role is more confined to mitochondrial functions, Trx1 plays a major role in protecting S. pombe against various stressful conditions and enables proper sulfur metabolism.
-
Citations
Citations to this article as recorded by - In fission yeast, 65 non-essential mitochondrial proteins related to respiration and stress become essential in low-glucose conditions
Ayaka Mori, Lisa Uehara, Yusuke Toyoda, Fumie Masuda, Saeko Soejima, Shigeaki Saitoh, Mitsuhiro Yanagida
Royal Society Open Science.2023;[Epub] CrossRef - Improvement of Root Yield and Ion Content of Carrot with Exogenous Application Calcium Under Salinity
Ahmet Turhan, Hayrettin Kuscu
Gesunde Pflanzen.2023; 75(4): 947. CrossRef - Pharmacologic approaches to amino acid depletion for cancer therapy
Carly S. Wilder, Zhao Chen, John DiGiovanni
Molecular Carcinogenesis.2022; 61(2): 127. CrossRef - Human Sulfotransferase Assays With PAPS Production in situ
Yanan Sun, Lukas Corbinian Harps, Matthias Bureik, Maria Kristina Parr
Frontiers in Molecular Biosciences.2022;[Epub] CrossRef - Response to sulfur in Schizosaccharomyces pombe
Hokuto Ohtsuka, Takafumi Shimasaki, Hirofumi Aiba
FEMS Yeast Research.2021;[Epub] CrossRef - Improvement in salt tolerance of Iris pseudacorus L. in constructed wetland by exogenous application of salicylic acid and calcium chloride
Yuanyuan Liu, Min Xi, Yue Li, Ziwei Cheng, Sen Wang, Fanlong Kong
Journal of Environmental Management.2021; 300: 113703. CrossRef - The Efficiency of Different Priming Agents for Improving Germination and Early Seedling Growth of Local Tunisian Barley under Salinity Stress
Rim Ben Youssef, Nahida Jelali, Nadia Boukari, Alfonso Albacete, Cristina Martinez, Francisco Perez Alfocea, Chedly Abdelly
Plants.2021; 10(11): 2264. CrossRef - Salicylic Acid and Calcium Signaling Induce Physiological and Phytochemical Changes to Improve Salinity Tolerance in Red Amaranth (Amaranthus tricolor L.)
Hai Ly Hoang, Constancio C. de Guzman, Nina M. Cadiz, Thi Thai Hoa Hoang, Dang Hoa Tran, H. Rehman
Journal of Soil Science and Plant Nutrition.2020; 20(4): 1759. CrossRef - Treatment of nitric oxide supplemented with nitrogen and sulfur regulates photosynthetic performance and stomatal behavior in mustard under salt stress
Badar Jahan, Mohamed F. AlAjmi, Md Tabish Rehman, Nafees A. Khan
Physiologia Plantarum.2020; 168(2): 490. CrossRef - Hemolymph transcriptome analysis of Chinese mitten crab (Eriocheir sinensis) with intact, left cheliped autotomy and bilateral eyestalk ablation
Cong Zhang, Yangyang Pang, Qian Zhang, Genyong Huang, Minjie Xu, Boping Tang, Yongxu Cheng, Xiaozhen Yang
Fish & Shellfish Immunology.2018; 81: 266. CrossRef - Lack of a peroxiredoxin suppresses the lethality of cells devoid of electron donors by channelling electrons to oxidized ribonucleotide reductase
Susanna Boronat, Alba Domènech, Mercè Carmona, Sarela García-Santamarina, M. Carmen Bañó, José Ayté, Elena Hidalgo, Julian E. Sale
PLOS Genetics.2017; 13(6): e1006858. CrossRef - Ethephon increases photosynthetic-nitrogen use efficiency, proline and antioxidant metabolism to alleviate decrease in photosynthesis under salinity stress in mustard
Noushina Iqbal, Shahid Umar, Tasir S. Per, Nafees A. Khan
Plant Signaling & Behavior.2017; 12(5): e1297000. CrossRef - Thioredoxins are involved in the activation of the PMK1 MAP kinase pathway during appressorium penetration and invasive growth in Magnaporthe oryzae
Shijie Zhang, Cong Jiang, Qiang Zhang, Linlu Qi, Chaohui Li, Jin‐Rong Xu
Environmental Microbiology.2016; 18(11): 3768. CrossRef - l‐Cysteine metabolism and its nutritional implications
Jie Yin, Wenkai Ren, Guan Yang, Jielin Duan, Xingguo Huang, Rejun Fang, Chongyong Li, Tiejun Li, Yulong Yin, Yongqing Hou, Sung Woo Kim, Guoyao Wu
Molecular Nutrition & Food Research.2016; 60(1): 134. CrossRef - Diverse fission yeast genes required for responding to oxidative and metal stress: Comparative analysis of glutathione‐related and other defense gene deletions
Tomáš Pluskal, Kenichi Sajiki, Joanne Becker, Kojiro Takeda, Mitsuhiro Yanagida
Genes to Cells.2016; 21(6): 530. CrossRef - Nitrogen availability regulates proline and ethylene production and alleviates salinity stress in mustard (Brassica juncea)
Noushina Iqbal, Shahid Umar, Nafees A. Khan
Journal of Plant Physiology.2015; 178: 84. CrossRef - Subcellular localization of six thioredoxins and their antioxidant activity and contributions to biological control potential in Beauveria bassiana
Long-Bin Zhang, Li Tang, Sheng-Hua Ying, Ming-Guang Feng
Fungal Genetics and Biology.2015; 76: 1. CrossRef - Simultaneous application of salicylic acid and calcium improves salt tolerance in two contrasting tomato (Solanum lycopersicum) cultivars
A. Manaa, E. Gharbi, H. Mimouni, S. Wasti, S. Aschi-Smiti, S. Lutts, H. Ben Ahmed
South African Journal of Botany.2014; 95: 32. CrossRef - The Oxidative Stress Responsive Transcription Factor Pap1 Confers DNA Damage Resistance on Checkpoint-Deficient Fission Yeast Cells
Carrie Belfield, Craig Queenan, Hui Rao, Kenji Kitamura, Nancy C. Walworth, Deanna M. Koepp
PLoS ONE.2014; 9(2): e89936. CrossRef - Possible Involvement of Nitric Oxide and Reactive Oxygen Species in Glucose Deprivation-Induced Activation of Transcription Factor Rst2
Toshiaki Kato, Xin Zhou, Yan Ma, Reiko Sugiura
PLoS ONE.2013; 8(10): e78012. CrossRef - Effect of Salinity and Calcium on Tomato Fruit Proteome
Arafet Manaa, Mireille Faurobert, Benoît Valot, Jean-Paul Bouchet, Dominique Grasselly, Mathilde Causse, Hela Ben Ahmed
OMICS: A Journal of Integrative Biology.2013; 17(6): 338. CrossRef - The transcription factors Pap1 and Prr1 collaborate to activate antioxidant, but not drug tolerance, genes in response to H 2 O 2
Isabel A. Calvo, Patricia García, José Ayté, Elena Hidalgo
Nucleic Acids Research.2012; 40(11): 4816. CrossRef - Txl1 and Txc1 Are Co-Factors of the 26S Proteasome in Fission Yeast
Katrine M. Andersen, Camilla Jensen, Franziska Kriegenburg, Anne-Marie B. Lauridsen, Colin Gordon, Rasmus Hartmann-Petersen
Antioxidants & Redox Signaling.2011; 14(9): 1601. CrossRef - Salt-Stress Induced Physiological and Proteomic Changes in Tomato (Solanum lycopersicum) Seedlings
Arafet Manaa, Hela Ben Ahmed, Samira Smiti, Mireille Faurobert
OMICS: A Journal of Integrative Biology.2011; 15(11): 801. CrossRef -
Thiol-Independent Action of Mitochondrial Thioredoxin To Support the Urea Cycle of Arginine Biosynthesis in
Schizosaccharomyces pombe
Ji-Yoon Song, Kyoung-Dong Kim, Jung-Hye Roe
Eukaryotic Cell.2008; 7(12): 2160. CrossRef
- Partial Purification of Factors for Differential Transcription of the rrnD Promoters for Ribosomal RNA Synthesis in Streptomyces coelicolor
-
Mi-Young Hahn , Jung-Hye Roe
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J. Microbiol. 2007;45(6):534-540.
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DOI: https://doi.org/2612 [pii]
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Abstract
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The Streptomyces coelicolor A3(2) genome contains six operons (rrnA to F) for ribosomal RNA synthesis. Transcription from rrnD occurs from four promoters (p1 to p4). We found that transcripts from the p1 and p3 promoters were most abundant in vivo in the early exponential phase. However, at later phases of exponential and stationary growth, transcripts from the p1 promoter decreased drastically, with the p3 and p4 transcripts constituting the major forms. Partially purified RNA polymerase supported transcription from the p3 and p4 promoters, whereas pure reconstituted RNA polymerase with core enzyme (E) and the major vegetative sigma factor sigmaHrdB (E.sigmaHrdB) did not. In order to assess any potential requirement for additional factor(s) that allow transcription from the p3 and p4 promoters, we fractionated a partially purified RNA polymerase preparation by denaturing gel filtration chromatography. We found that transcription from the p3 and p4 promoters required factor(s) of about 30-35 kDa in addition to RNAP holoenzyme (E.sigmaHrdB). Therefore, transcription from the p3 and p4 promoters, which contain a consensus -10 region but no -35 for sigmaHrdB recognition, are likely to be regulated by transcription factor(s) that modulate RNA polymerase holoenzyme activity in S. coelicolor.
- Identification of Genes for Mycothiol Biosynthesis in Streptomyces coelicolor A3(2)
-
Joo-Hong Park , Chang-Jun Cha , Jung-Hye Roe
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J. Microbiol. 2006;44(1):121-125.
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DOI: https://doi.org/2327 [pii]
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Abstract
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Mycothiol is a low molecular weight thiol compound produced by a number of actinomycetes, and
has been suggested to serve both anti-oxidative and detoxifying roles. To investigate the metabolism
and the role of mycothiol in Streptomyces coelicolor, the biosynthetic genes (mshA, B, C, and
D) were predicted based on sequence homology with the mycobacterial genes and confirmed
experimentally. Disruption of the mshA, C, and D genes by PCR targeting mutagenesis resulted
in no synthesis of mycothiol, whereas the mshB mutation reduced its level to about 10% of the
wild type. The results indicate that the mshA, C, and D genes encode non-redundant biosynthetic
enzymes, whereas the enzymatic activity of MshB (acetylase) is shared by at least one other gene
product, most likely the mca gene product (amidase).
- Identification of [sigma]^B-Dependent Promoters Using Consensus-Directed Search of Streptomyces coelicolor Genome
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Eun-Jin Lee , You-Hee Cho , Hyo-Sub Kim , Jung-Hye Roe
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J. Microbiol. 2004;42(2):147-151.
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DOI: https://doi.org/2030 [pii]
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Abstract
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[sigma]^B plays an important role in both osmoprotection and proper differentiation in Streptomyces coelicolor A3(2). We searched for candidate members of the [sigma]^B regulon from the genome database, using the consensus promoter sequence (GNNTN_14-16GGGTAC/T). The list consists of 115 genes, and includes all the known [sigma]^B target genes and many other genes whose functions are related to stress protection and differentiation.
- Regulation of the sufABCDSE Operon by Fur
-
Joon-Hee Lee , Won-Sik Yeo , Jung-Hye Roe
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J. Microbiol. 2003;41(2):109-114.
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Abstract
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A promoter that is inducible by paraquat and menadione, the superoxide generators, independently of soxRS has been found in front of the sufABCDSE operon in Escherichia coli. Based on the observation that SufA is a holomog of IscA that functions in the assembly of iron sulfur cluster and the sufA promoter (sufAp) contains a putative Fur-binding consensus, we investigated whether this gene is regulated by Fur, a ferric uptake regulator. When examined in several sufAp-lacZ chromosomal fusion strains, sufAp was induced by EDTA, an iron chelator and a well-known Fur-inducer. The basal level of sufA expression increased dramatically in fur mutant, suggesting repression of sufAp by Fur. The derepression in fur mutant and EDTA-induction of sufA expression required nucleotides up to -61, where a putative Fur box is located. Purified Fur protein bound to the DNA fragment containing the putative Fur box between -35 and -10 promoter elements. The regulation by Fur and menadione induction of sufAp acted independently. The rpoS mutation increased sufA induction by menadione, suggesting that the stationary sigma factor RpoS acts negatively on sufA induction.
- Isolation and Characterization of the sod2+ Gene Encoding a Putative Mitochondrial Manganese Superoxide Dismutase in Schizosaccharomyces pombe
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Jae-Hoon Jeong , Eun-Soo Kwon , Jung-Hye Roe
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J. Microbiol. 2001;39(1):37-41.
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Abstract
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The fission yeast Schizosaccharomyces pombe contains two distinct superoxide dismutase (SOD) activities, one in the cytosol encoded by the sod1+ gene and the other in mitochondria. The sod2+ gene encoding putative mitochondrial manganese superoxide dismutase (MnSOD) was isolated from the S. pombe genomic library using a PCR fragment as the probe. The nucleotide sequence of the sod2+ gene and its flanking region (4051 bp HindIII fragment) was determined. An intron of 123 nt in size was predicted and confirmed by sequencing the cDNA following reverse transcription PCR. The predicted Sod2p consists of 218 amino acid residues with a molecular mass of 24,346 Da. The deduced amino acid sequence showed a high degree of homology with other MnSODs, especially in the metal binding residues at the active site and their relative positions. The transcriptional start site was mapped by primer extension at 231 nt upstream from the ATG codon. A putative TATA box (TATAAAA) was located 58 nt upstream from the transcriptional start site and putative polyadenylation sites were located at 1000, 1062, and 1074 nt downstream from the ATG start codon.
- Analysis of the Dual Promoters and the H 2 O 2 -responsive Element of the catA Gene Encoding Catalase A in Streptomyces coelicolor
-
You-Hee Cho , Ji-Sook Hahn , Jung-Hye Roe
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J. Microbiol. 2000;38(4):239-244.
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Abstract
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The catA gene encodes the major catalase in Streptomyces coelicolor, whose production increases upon H_2O_2 treatment. Besides the previously identified primary promoter (catAp1), a minor promoter (catAp2) was newly assigned by S1 nuclease mapping. The catAp2 transcript was observed transiently upon entry into the stationary phase in liquid culture and upon differentiation on solid plates, whereas the level of catAp1 transcription did not change significantly during this growth transition. The catAp1 promoter was transcribed by the major vegetative RNA polymerase holoenzyme containing [sigma]^HrdB , whereas the catAp2 was transcribed in vitro by the holoenzyme containing [sigma]^R that is activated under oxidative conditions. The cis-element regulating the H_2 O_2 -inducibility of catAp1 was identified within the 23 bp inverted repeat sequence located between -65 and -43 of the catAp1 promoter. We named this sequence HRE (H_2O_2 -responsive element). The distal half of the inverted repeat was more crucial for H_2 O_2 ?pendent induction of the catAp1 transcript than the proximal half. HRE most likely serves as a binding site for the H_2 O_2 -responsive repressor CatR.
- Subcellular Localization of Catalase Encoded by the ctt1^+ Gene in Schizosaccharomyces pombe
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Sang-il Lee , Joon Lee , Jung-Hye Roe
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J. Microbiol. 2000;38(3):156-159.
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Abstract
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The ctt1^+ gene in Schizosaccharomyces pombe encodes a catalase responsible for H_2O_2 -resistance of this organism as judged by the H_2O_2 -sensitive phenotype of the ctt1[delta] mutant. In this study, we investigated the subcellular localization of the Ctt1 gene product. In wild type cells catalase activity was detected in the organelle fraction as well as in the cytosol. The ctt1[delta] mutant contained no catalase activity, indicating that both cytosolic and organellar catalases are the products of a single ctt1^+ gene. Western blot analysis revealed two catalase bands, both of which disappeared in the ctt1[delta] mutant. The major, faster-migrating band existed in the cytosol whereas the minor, slower-migrating band appeared to be located in organelles, most likely in peroxisomes. These results suggest that the ctt1^++ gene product targeted to the peroxisome is a modified form of the one in the cytosol.
- Isolation of the Regulator Gene Responsible for Overproduction of Catalase A in H 2 O 2 -resistant Mutant of Streptomyces coelicolor
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Ji-Sook Hahn , So-Young Oh , Keith F. Chater , Jung-Hye Roe
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J. Microbiol. 2000;38(1):18-23.
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Abstract
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Streptomyces coelicolor produces three kinds of catalases to cope with oxidative stress and to allow nor-mal differentiation. Catalase A is the major vegetative catalase which functions in removing hydrogen peroxide generated during the process of aerobic metabolism. To understand the regulatory mechanism of response against oxidative stress, hydrogen peroxide-resistant mutant (HR40) was isolated from S. coelicolor J1501 following UV mutagenesis. The mutant overproduced catalase A more than 50-fold compared with the wild type. The mutation locus catR1 was mapped closed to the mthB2 locus by genetic crossings. An ordered cosmid library of S. coelicolor encompassing the mthB2 locus was used to isolate the regulator gene (catR) which represses catalase overproduction when introduced into HR40. A candidate catR gene was found to encode a Fur-like protein of 138 amino acids (15319 Da).
- Function of mORF1 Protein as a Terminal Recognition Factor for the Linear Mitochondrial Plasmid pMLP1 from Pleurotus ostreatus
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Eun-Kyoung Kim , Jung-Hye Roe
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J. Microbiol. 1999;37(4):229-233.
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Abstract
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The mitochondrial plasmid pMLP1 from a white-rot fungus, Pleurotus ostreatus, is a double-stranded DNA containing 381 bp terminal inverted repeat (TIR) whose 5'-ends are covalently bound by terminal proteins. The plasmid contains two major open reading frames (ORFs), encoding putative DNA and RNA polymerases, and a minor ORF encoding a small, highly basic protein. To identify the DNA binding activity that recognizes the TIR region of pMLP1, gel retardation assays were performed with mitochondrial extracts. A specific protein binding to a region between 123 and 248 nt within TIR was observed. We examined whether the gene product of mORF1 bindes to this region specifically. E. coli cell extract which contains an overproduced mORF1 protein formed a complex specific to the region between 123 and 248 nt. Inclusion of mORF1 protein in the specific complex formed between P. ostreatus mitochondrial extract and TIR was confirmed by a supershift assay using polyclonal antibodies against the mORF1 protein. Our result suggest that the product of mORF1 may function as a terminal region recognition factor (TRF), recognizing an internal region in TIR.
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MSK
