- Improved tolerance of recombinant Chlamydomonas rainhardtii with putative 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase from Pyropia yezoensis to nitrogen starvation
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Seo-jeong Park , Joon Woo Ahn , Jong-il Choi
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J. Microbiol. 2022;60(1):63-69. Published online December 29, 2021
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DOI: https://doi.org/10.1007/s12275-022-1491-7
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 Abstract
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In a previous study, a putative 2-amino-3-carboxymuconate-
6-semialdehyde decarboxylase (ACMSD) was highly expressed
in a mutant strain of Pyropia yezoensis, which exhibited an
improved growth rate compared to its wild strain. To investigate
the functional role of the putative ACMSD (Pyacmsd)
of P. yezoensis, the putative Pyacmsd was cloned and expressed
in Chlamydomonas reinhardtii. Recombinant C. reinhardtii
cells with Pyacmsd (Cr_Pyacmsd) exhibited enhanced tolerance
compared to control C. reinhardtii cells (Cr_control)
under nitrogen starvation. Notably, Cr_Pyacmsd cells showed
accumulation of lipids in nitrogen-enriched conditions. These
results
demonstrate the role of Pyacmsd in the generation of
acetyl-coenzyme A. Thus, it can be used to enhance the production
of biofuel using microalgae such as C. reinhardtii and
increase the tolerance of other biological systems to nitrogendeficient
conditions.
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Citations
Citations to this article as recorded by  - Characteristics of Recombinant Chlamydomonas reinhardtii Expressing Putative Germin-Like Protein from Neopyropia yezoensis
Jiae Kim, Jong-il Choi
Journal of Microbiology and Biotechnology.2024; 34(10): 2132. CrossRef - Overexpression of putative glutathione peroxidase from Neopyropia-associated microorganisms in Chlamydomonas to respond to abiotic stress
Jeong Hyeon Kim, Eun-Jeong Park, Jong-il Choi
Archives of Microbiology.2023;[Epub] CrossRef - Overexpression of S-Adenosylmethionine Synthetase in Recombinant Chlamydomonas for Enhanced Lipid Production
Jeong Hyeon Kim, Joon Woo Ahn, Eun-Jeong Park, Jong-il Choi
Journal of Microbiology and Biotechnology.2023; 33(3): 310. CrossRef
- Improved tolerance of Escherichia coli to oxidative stress by expressing putative response regulator homologs from Antarctic bacteria
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Seo-jeong Park , Sangyong Lim , Jong-il Choi
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J. Microbiol. 2020;58(2):131-141. Published online December 23, 2019
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DOI: https://doi.org/10.1007/s12275-020-9290-5
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Abstract
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Response regulator (RR) is known a protein that mediates
cell’s response to environmental changes. The effect of RR
from extremophiles was still under investigation. In this study,
response regulator homologs were mined from NGS data
of Antarctic bacteria and overexpressed in Escherichia coli.
Sixteen amino acid sequences were annotated corresponding
to response regulators related to the two-component regulatory
systems; of these, 3 amino acid sequences (DRH632,
DRH1601 and DRH577) with high homology were selected.
These genes were cloned in pRadGro and expressed in E. coli.
The transformant strains were subjected to various abiotic
stresses including oxidative, osmotic, thermal stress, and acidic
stress. There was found that the robustness of E. coli to
abiotic stress was increased in the presence of these response
regulator homologs. Especially, recombinant E. coli overexpressing
drh632 had the highest survival rate in oxidative,
hypothermic, osmotic, and acidic conditions. Recombinant E.
coli overexpressing drh1601 showed the highest tolerance level
to osmotic stress. These results will be applicable for development
of recombinant strains with high tolerance to abiotic
stress.
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Citations
Citations to this article as recorded by -
Mechanistic and bibliometric insights into
RpoS
-mediated biofilm regulation and its strategic role in food safety applications
Shirin Akter, Md. Ashikur Rahman, Md. Ashrafudoulla, A.G.M.Sofi Uddin Mahamud, Md Anamul Hasan Chowdhury, Sang-Do Ha
Critical Reviews in Food Science and Nutrition.2025; : 1. CrossRef - Deionococcus proteotlycius Genomic Library Exploration Enhances Oxidative Stress Resistance and Poly-3-hydroxybutyrate Production in Recombinant Escherichia coli
Seul-Ki Yang, Soyoung Jeong, Inwoo Baek, Jong-il Choi, Sangyong Lim, Jong-Hyun Jung
Microorganisms.2023; 11(9): 2135. CrossRef - Bacterial redox response factors in the management of environmental oxidative stress
Sudharsan M, Rajendra Prasad N, Saravanan Rajendrasozhan
World Journal of Microbiology and Biotechnology.2023;[Epub] CrossRef - Bacteriophages as Antimicrobial Agents? Proteomic Insights on Three Novel Lytic Bacteriophages Infecting ESBL-Producing Escherichia coli
Sadika Dkhili, Miguel Ribeiro, Salma Ghariani, Houssem Ben Yahia, Mélanie Hillion, Patricia Poeta, Karim Ben Slama, Michel Hébraud, Gilberto Igrejas
OMICS: A Journal of Integrative Biology.2021; 25(10): 626. CrossRef - Regulator of ribonuclease activity modulates the pathogenicity of Vibrio vulnificus
Jaejin Lee, Eunkyoung Shin, Jaeyeong Park, Minho Lee, Kangseok Lee
Journal of Microbiology.2021; 59(12): 1133. CrossRef
- PprM is necessary for up-regulation of katE1, encoding the major catalase of Deinococcus radiodurans, under unstressed culture conditions
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Sun-Wook Jeong , Ho Seong Seo , Min-Kyu Kim , Jong-Il Choi , Heon-Man Lim , Sangyong Lim
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J. Microbiol. 2016;54(6):426-431. Published online May 27, 2016
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DOI: https://doi.org/10.1007/s12275-016-6175-8
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89
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Abstract
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Deinococcus radiodurans is a poly-extremophilic organism,
capable of tolerating a wide variety of different stresses, such
as gamma/ultraviolet radiation, desiccation, and oxidative
stress. PprM, a cold shock protein homolog, is involved in
the radiation resistance of D. radiodurans, but its role in the
oxidative stress response has not been investigated. In this
study, we investigated the effect of pprM mutation on catalase
gene expression. pprM disruption decreased the mRNA and
protein levels of KatE1, which is the major catalase in D. radiodurans,
under normal culture conditions. A pprM mutant
strain (pprMMT) exhibited decreased catalase activity, and its
resistance to hydrogen peroxide (H2O2) decreased accordingly
compared with that of the wild-type strain. We confirmed
that RecG helicase negatively regulates katE1 under normal
culture conditions. Among katE1 transcriptional regulators,
the positive regulator drRRA was not altered in pprM-, while
the negative regulators perR, dtxR, and recG were activated
more than 2.5-fold in pprMMT. These findings suggest that
PprM is necessary for KatE1 production under normal culture
conditions by down-regulation of katE1 negative regulators.
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Citations
Citations to this article as recorded by -
Antioxidant defense of
Deinococcus radiodurans
: how does it contribute to extreme radiation resistance?
Izabela Sadowska-Bartosz, Grzegorz Bartosz
International Journal of Radiation Biology.2023; 99(12): 1803. CrossRef - Development and Regulation of the Extreme Biofilm Formation of Deinococcus radiodurans R1 under Extreme Environmental Conditions
Qiannan Guo, Yuhua Zhan, Wei Zhang, Jin Wang, Yongliang Yan, Wenxiu Wang, Min Lin
International Journal of Molecular Sciences.2023; 25(1): 421. CrossRef - A small RNA regulates pprM, a modulator of pleiotropic proteins promoting DNA repair, in Deinococcus radiodurans under ionizing radiation
Jordan K. Villa, Runhua Han, Chen-Hsun Tsai, Angela Chen, Philip Sweet, Gabriela Franco, Respina Vaezian, Rok Tkavc, Michael J. Daly, Lydia M. Contreras
Scientific Reports.2021;[Epub] CrossRef - Lack of the Bacterial Phytochrome Protein Decreases Deinococcus radiodurans Resistance to Mitomycin C
Jong-Hyun Jung, Soyoung Jeong, Seonghun Im, Min-Kyu Kim, Ho Seong Seo, Sangyong Lim
Frontiers in Microbiology.2021;[Epub] CrossRef - Effects of Conserved Wedge Domain Residues on DNA Binding Activity of Deinococcus radiodurans RecG Helicase
Sun-Wook Jeong, Min-Kyu Kim, Lei Zhao, Seul-Ki Yang, Jong-Hyun Jung, Heon-Man Lim, Sangyong Lim
Frontiers in Genetics.2021;[Epub] CrossRef - The Novel ncRNA OsiR Positively Regulates Expression of katE2 and is Required for Oxidative Stress Tolerance in Deinococcus radiodurans
Lihua Gao, Xiaonan Chen, Ye Tian, Yongliang Yan, Yuhua Zhan, Zhengfu Zhou, Wei Zhang, Min Lin, Ming Chen
International Journal of Molecular Sciences.2020; 21(9): 3200. CrossRef - Conservation and diversity of radiation and oxidative stress resistance mechanisms inDeinococcusspecies
Sangyong Lim, Jong-Hyun Jung, Laurence Blanchard, Arjan de Groot
FEMS Microbiology Reviews.2019; 43(1): 19. CrossRef - Gene regulation for the extreme resistance to ionizing radiation of Deinococcus radiodurans
Wuzhou Wang, Yun Ma, Junyan He, Huizhou Qi, Fangzhu Xiao, Shuya He
Gene.2019; 715: 144008. CrossRef - PprM, a Cold Shock Domain-Containing Protein from Deinococcus radiodurans, Confers Oxidative Stress Tolerance to Escherichia coli
Sun-Ha Park, Harinder Singh, Deepti Appukuttan, Sunwook Jeong, Yong Jun Choi, Jong-Hyun Jung, Issay Narumi, Sangyong Lim
Frontiers in Microbiology.2017;[Epub] CrossRef - Knockout of pprM Decreases Resistance to Desiccation and Oxidation in Deinococcus radiodurans
Yang Zeng, Yun Ma, Fangzhu Xiao, Wuzhou Wang, Shuya He
Indian Journal of Microbiology.2017; 57(3): 316. CrossRef - RNA-Binding Domain is Necessary for PprM Function in Response to the Extreme Environmental Stress in Deinococcus radiodurans
Wei Li, Yun Ma, Jie Yang, Fangzhu Xiao, Wuzhou Wang, Shuya He
Indian Journal of Microbiology.2017; 57(4): 492. CrossRef
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