- Descr!ption of Ornithinimicrobium cryptoxanthini sp. nov., a Novel Actinomycete Producing β‑cryptoxanthin Isolated from the Tongtian River Sediments
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Yuyuan Huang , Yifan Jiao , Sihui Zhang , Yuanmeihui Tao , Suping Zhang , Dong Jin , Ji Pu , Liyun Liu , Jing Yang , Shan Lu
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J. Microbiol. 2023;61(4):379-388. Published online March 16, 2023
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DOI: https://doi.org/10.1007/s12275-023-00029-5
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Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and
JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37′13″N, 96°05′37″E)
in July 2019. Both strains were shown to grow at 15–35 °C and pH 7.0–10.0, and in the presence of 0–6.0% (w/v) NaCl.
The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH
30183
T (98.6–98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T
(98.5–98.6%) and O. murale 01-Gi-040T
(98.3–98.5%). The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively,
revealed that the two strains formed a distinct cluster with the above three species. The digital DNA-DNA hybridization
(dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other
Ornithinimicrobium species were within the ranges of 19.0–23.9% and 70.8–80.4%, respectively, all below the respective
recommended 70.0% and 95–96% cutoff point. Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and
JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9. Strain JY.X270T contained MK-8(H4) and ornithine as the predominant
menaquinone and diagnostic diamino acid component within the cell wall teichoic acids. β-cryptoxanthin (
C40H56O) can
be extracted from strain JY.X270T, and its content is 6.3 μg/ml. Based on results from the phylogenetic, chemotaxonomic,
and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which
the name Ornithinimicrobium cryptoxanthini sp. nov. is proposed (type strain JY.X270T = CGMCC 1.19147T = JCM 34882T).
-
Citations
Citations to this article as recorded by  -
Microbacterium jiangjiandongii sp. nov. and Microbacterium wangruii sp. nov., two β-cryptoxanthin-producing bacteria isolated from intestinal contents of Marmota himalayana
Lin Ye, Xiaorui Liu, Jing Yang, Ji Pu, Caixin Yang, Gui Zhang, Juan Zhou, Yue Liu, Dong Jin, Shan Lu, Jianguo Xu
International Journal of Systematic and Evolutionary Microbiology
.2025;[Epub] CrossRef - Screening, identification, and characterization of high potential bacteria for ꞵ-cryptoxanthin production from natural sources
Sopida Korkerd, Savitri Vatanyoopaisarn, Wonnop Visessaguan, Benjawan Thumthanarak, Dudsadee Uttapap, Solange I. Mussatto, Vilai Rungsardthong
Biocatalysis and Agricultural Biotechnology.2024; 57: 103089. CrossRef
- Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp. nov., a novel halotolerant actinomycete isolated from bat feces
-
Yuyuan Huang , Lingzhi Dong , Jian Gong , Jing Yang , Shan Lu , Xin-He Lai , Dong Jin , Qianni Huang , Ji Pu , Liyun Liu , Jianguo Xu
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J. Microbiol. 2022;60(10):977-985. Published online August 19, 2022
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DOI: https://doi.org/10.1007/s12275-022-2134-8
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367
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Two strictly aerobic, Gram-staining-positive, non-spore-forming,
regular rod-shaped (approximately 0.7 × 1.9 mm)
bacteria (HY170T and HY001) were isolated from bat feces
collected from Chongzuo city, Guangxi province (22°2054N,
106°4920E, July 2011) and Chuxiong Yi Autonomous Prefecture,
Yunnan province (25°0910N, 102°0439E, October
2013) of South China, respectively. Optimal growth is obtained
at 25–28°C (range, 4–32°C) on BHI-5% sheep blood
plate with pH 7.5 (range, 5.0–10.0) in the presence of 0.5–
1.0% NaCl (w/v) (range, 0–15% NaCl [w/v]). The phylogenetic
and phylogenomic trees based respectively on the 16S
rRNA gene and 845 core gene sequences revealed that the
two strains formed a distinct lineage within the genus Brevibacterium,
most closely related to B. aurantiacum NCDO
739T (16S rRNA similarity, both 98.5%; dDDH, 46.7–46.8%;
ANI, 91.9–92.1%). Strain HY170T contained MK-8(H2), diphosphatidylglycerol
(DPG) and phosphatidylglycerol (PG),
galactose and ribose as the predominant menaquinone, major
polar lipids, and main sugars in the cell wall teichoic acids,
respectively. The meso-diaminopimelic acid (meso-DAP)
was the diagnostic diamino acid of the peptidoglycan found
in strain HY170T. Anteiso-C15:0 and anteiso-C17:0 were the
major fatty acids (> 10%) of strains HY170T and HY001, with
anteiso-C17:1A predominant in strain HY170T but absent in
strain HY001. Mining the genomes revealed the presence
of secondary metabolite biosynthesis gene clusters encoding
for non-alpha poly-amino acids (NAPAA), ectoine, siderophore,
and terpene. Based on results from the phylogenetic,
chemotaxonomic and phenotypic analyses, the two strains
could be classified as a novel species of the genus Brevibacterium,
for which the name Brevibacterium zhoupengii sp.
nov. is proposed (type strain HY170T = CGMCC 1.18600T
= JCM 34230T).
-
Citations
Citations to this article as recorded by - Comparative metagenomics of wild and cultivated Fragaria chiloensis reveals major rhizosphere microbiome shifts linked to stress adaptation
Carlos Farkas, Matías Guerra, Adan Andreu Heredia, Jean Franco Castro
Current Research in Microbial Sciences.2025; 9: 100460. CrossRef - Antagonistic Behavior of Streptomyces chartreuse against Pathogenic Bacteria in Ricinus communis L.
Bhoomi N. Patel, Priti Patel, Gayatri Patel
Biosciences Biotechnology Research Asia.2024; 21(1): 185. CrossRef - Functional genomics and taxonomic insights into heavy metal tolerant novel bacterium Brevibacterium metallidurans sp. nov. NCCP-602T isolated from tannery effluent in Pakistan
Sadia Manzoor, Saira Abbas, Sobia Zulfiqar, Hong-Chuan Wang, Min Xiao, Wen-Jun Li, Muhammad Arshad, Iftikhar Ahmed
Antonie van Leeuwenhoek.2024;[Epub] CrossRef - Description of Ornithinimicrobium cryptoxanthini sp. nov., a Novel Actinomycete Producing β-cryptoxanthin Isolated from the Tongtian River Sediments
Yuyuan Huang, Yifan Jiao, Sihui Zhang, Yuanmeihui Tao, Suping Zhang, Dong Jin, Ji Pu, Liyun Liu, Jing Yang, Shan Lu
Journal of Microbiology.2023; 61(4): 379. CrossRef - Morphological and genomic characteristics of two novel actinomycetes, Ornithinimicrobium sufpigmenti sp. nov. and Ornithinimicrobium faecis sp. nov. isolated from bat faeces (Rousettus leschenaultia and Taphozous perforates)
Yuyuan Huang, Suping Zhang, Yuanmeihui Tao, Jing Yang, Shan Lu, Dong Jin, Ji Pu, Wenbo Luo, Han Zheng, Liyun Liu, Jia-fu Jiang, Jianguo Xu
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef
- Flaviflexus equikiangi sp. nov. isolated from faeces of Equus kiang (Tibetan wild ass) and carrying a class 1 integron gene cassette in its genome
-
Caixin Yang , Xingxing Lian , Yanpeng Cheng , Yifan Jiao , Jing Yang , Kui Dong , Shan Lu , Xin-He Lai , Dong Jin , Han Zheng , Ji Pu , Suping Wang , Liyun Liu , Jianguo Xu
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J. Microbiol. 2022;60(6):585-593. Published online April 18, 2022
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DOI: https://doi.org/10.1007/s12275-022-1673-3
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Abstract
PDF
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Two Gram-stain-positive, catalase-negative, non-spore-forming,
cocci-shaped strains (dk850T and JY899) were isolated
from the feces of Equus kiang in the Qinghai-Tibet Plateau of
China. 16S rRNA gene sequence-based phylogenetic analyses
showed that strains dk850T and JY899 belong to the genus
Flaviflexus, closest to F. salsibiostraticola KCTC 33148T, F. ciconiae
KCTC 49253T and F. huanghaiensis H5T. The DNA
G + C content of strain dk850T was 62.9%. The digital DNADNA
hybridization values of strain dk850T with the closely related
species were below the 70% threshold for species demarcation.
The two strains grew best at 28°C on brain heart infusion
(BHI) agar with 5% sheep blood. All strains had C18:1ω9c
and C16:0 as the major cellular fatty acids. MK-9(H4) was the
major menaquinone in strain dk850T. The major polar lipids
included diphosphatidylglycerol and an unidentified phospholipid.
Strains dk850T and JY899 were identified as carrying
a class 1 integron containing the aminoglycoside resistance
gene aadA11, both strains were resistant to spectinomycin
and streptomycin. Based on several lines of evidence
from phenotypic and phylogenetic analyses, strains dk850T
and JY899 represent a novel species of the genus Flaviflexus,
for which the name Flaviflexus equikiangi sp. nov. is proposed.
The type strain is dk850T (= CGMCC 1.16593T = JCM 33598T).
- Changpingibacter yushuensis gen. nov., sp. nov., isolated from fluvial sediment in Qinghai Tibet Plateau of China
-
Yifan Jiao , Sihui Zhang , Jing Yang , Xin-He Lai , Kui Dong , Yanpeng Cheng , Mingchao Xu , Wentao Zhu , Shan Lu , Dong Jin , Ji Pu , Ying Huang , Liyun Liu , Suping Wang , Jianguo Xu
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J. Microbiol. 2022;60(2):147-155. Published online January 7, 2022
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DOI: https://doi.org/10.1007/s12275-022-1199-8
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324
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Abstract
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Two facultatively anaerobic, short rod-shaped, non-motile,
Gram-stain-positive, unknown bacterial strains (JY-X040T
and JY-X174) were isolated from fluvial sediments of Tongtian
River in Yushu Tibetan Autonomous Prefecture, Qinghai
province, China. Cells formed translucent, gray, round and
convex colonies, with a diameter of less than 0.5 mm after 5
days of incubation at 30°C on brain heart infusion-5% sheep
blood agar. The 16S rRNA gene sequence similarity between
strain JY-X040T and Fudania jinshanensis 313T is 93.87%.
In the four phylogenetic trees constructed based on the 16S
rRNA gene and 423 core genes, the two isolates form an independent
branch, phylogenetically closest to F. jinshanensis
313T, but could not be classified as a member of the genus
Fudania or any other genus of the family Arcanobacteriaceae.
The DNA G + C content of strain JY-X040T was 57.8%. Calculation
results
of average nucleotide identity, digital DNADNA
hybridization value and amino acid identity between
strain JY-X040T and F. jinshanensis 313T are 69.9%, 22.9%,
and 64.1%. The major cellular fatty acids were C16:0 (23%)
and C18:1ω9c (22%). The cell-wall peptidoglycan type was A5α
(L-Lys-L-Ala-L-Lys-D-Glu). The polar lipids comprised diphosphatidylglycerol,
phosphatidylglycerol, phosphatidylinositol,
phosphatidylinositol mannoside and four unidentified components.
The whole-cell sugars contained rhamnose and ribose.
MK-10(H4) was the sole respiratory quinone. The minimum
inhibitory concentration of streptomycin was 32 μg/ml. All
physiological, biochemical, chemotaxonomic and genomic
characteristics support that strains JY-X040T and JY-X174
represent members of a novel species in a new genus, Changpingibacter
yushuensis gen. nov., sp. nov. The type strain is
JY-X040T (GDMCC 1.1996T = KCTC 49514T).
-
Citations
Citations to this article as recorded by - Metagenomic and Culturomics Analysis of Microbial Communities within Surface Sediments and the Prevalence of Antibiotic Resistance Genes in a Pristine River: The Zaqu River in the Lancang River Source Region, China
Yi Yan, Jialiang Xu, Wenmin Huang, Yufeng Fan, Zhenpeng Li, Mingkai Tian, Jinsheng Ma, Xin Lu, Jian Liang
Microorganisms.2024; 12(5): 911. CrossRef - Bacterial diversity in arboreal ant nesting spaces is linked to colony developmental stage
Maximilian Nepel, Veronika E. Mayer, Veronica Barrajon-Santos, Dagmar Woebken
Communications Biology.2023;[Epub] CrossRef
- Isolation and characterization of tick-borne Roseomonas haemaphysalidis sp. nov. and rodent-borne Roseomonas marmotae sp. nov.
-
Wentao Zhu , Juan Zhou , Shan Lu , Jing Yang , Xin-He Lai , Dong Jin , Ji Pu , Yuyuan Huang , Liyun Liu , Zhenjun Li , Jianguo Xu
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J. Microbiol. 2022;60(2):137-146. Published online November 26, 2021
-
DOI: https://doi.org/10.1007/s12275-022-1428-1
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405
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Four novel Gram-negative, mesophilic, aerobic, motile, and
cocci-shaped strains were isolated from tick samples (strains
546T and 573) and respiratory tracts of marmots (strains 1318T
and 1311). The 16S rRNA gene sequencing revealed that strains
546T and 573 were 97.8% identical to Roseomonas wenyumeiae
Z23T, whereas strains 1311 and 1318T were 98.3% identical
to Roseomonas ludipueritiae DSM 14915T. In addition,
a 98.0% identity was observed between strains 546T and 1318T.
Phylogenetic and phylogenomic analyses revealed that strains
546T and 573 clustered with R. wenyumeiae Z23T, whereas
strains 1311 and 1318T grouped with R. ludipueritiae DSM
14915T. The average nucleotide identity between our isolates
and members of the genus Roseomonas was below 95%. The
genomic G+C content of strains 546T and 1318T was 70.9% and
69.3%, respectively. Diphosphatidylglycerol (DPG) and phosphatidylethanolamine
(PE) were the major polar lipids, with
Q-10 as the predominant respiratory quinone. According to
all genotypic, phenotypic, phylogenetic, and phylogenomic
analyses, the four strains represent two novel species of the
genus Roseomonas, for which the names Roseomonas haemaphysalidis
sp. nov. and Roseomonas marmotae sp. nov. are
proposed, with 546T (= GDMCC 1.1780T = JCM 34187T) and
1318T (= GDMCC 1.1781T = JCM 34188T) as type strains,
respectively.
-
Citations
Citations to this article as recorded by - Genomic epidemiology and antimicrobial resistance of Morganella clinical isolates between 2016 and 2023
Wentao Zhu, Qian Liu, Jinlv Liu, Yaqi Wang, Hong Shen, Ming Wei, Ji Pu, Li Gu, Jing Yang
Frontiers in Cellular and Infection Microbiology.2025;[Epub] CrossRef - Roseomonas populi sp. nov., an acetate-degrading bacteria isolated from the stem of Populus tomentosa
Yao Cheng, Wen Zhu, Shuo Han, Jingjing Yang, Guanqi Wu, Guozhu Zhao, Xiangwei He
Antonie van Leeuwenhoek.2024;[Epub] CrossRef - Description of Corynebacterium poyangense sp. nov., isolated from the feces of the greater white-fronted geese (Anser albifrons)
Qian Liu, Guoying Fan, Kui Wu, Xiangning Bai, Xi Yang, Wentao Song, Shengen Chen, Yanwen Xiong, Haiying Chen
Journal of Microbiology.2022; 60(7): 668. CrossRef -
Canibacter zhuwentaonis sp. nov. and Canibacter zhoujuaniae sp. nov., isolated from Marmota himalayana
Xianglian Lv, Yinmei Li, Yanpeng Cheng, Xin-He Lai, Jing Yang, Shan Lu, Gui Zhang, Caixin Yang, Dong Jin, Liyun Liu, Jianguo Xu
International Journal of Systematic and Evolutionary Microbiology
.2022;[Epub] CrossRef
- Agromyces laixinhei sp. nov. isolated from bat feces in China
-
Yanpeng Cheng , Yibo Bai , Yuyuan Huang , Jing Yang , Shan Lu , Dong Jin , Ji Pu , Han Zheng , Junqin Li , Ying Huang , Suping Wang , Jianguo Xu
-
J. Microbiol. 2021;59(5):467-475. Published online March 29, 2021
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DOI: https://doi.org/10.1007/s12275-021-0546-5
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377
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Three rod-shaped, Gram-stain-positive, and catalase-positive,
phenotypically closely related isolates (HY052T, HY050, and
HY045) were obtained from fecal samples collected from bats
in Guangxi province and Chongqing city of China. Circular,
smooth, light-yellow colonies appeared on brain heart infusion
plate after 24–48 h incubation at 28°C. The optimal pH
for growth was between 6.0 and 7.5. Based on 16S rRNA, the
three isolates were phylogenetically related to Agromyces terreus
DS-10T, Agromyces aureus AR33T, Agromyces salentinus
20-5T, Agromyces allii UMS-62T, Agromyces lapidis CD55T,
and Agromyces italicus CD1T. Moreover, based on 296 core
genes, the phylogenomic tree indicated that the three isolates
clustered together, closest to Agromyces cerinus VKM Ac-
1340T and Agromyces fucosus VKM Ac-1345T but separated
distantly from other Agromyces species. The average nucleotide
identity values between strain HY052T and other Agromyces
species ranged from 79.3% to 87.9%, lower than the
95–96% threshold. Furthermore, the genome of strain HY052T
contains a circular chromosome of 3,437,203 bp with G + C
content of 69.0 mol%. Main fatty acids were anteiso-C15:0
and anteiso-C17:0. The polar lipids comprised diphosphatidylglycerol,
phosphatidylglycerol, and unidentified glycolipids.
Rhamnose, ribose, and glucose were the primary cell wall sugars.
The major peptidoglycan amino acids included alanine,
glutamic acid, glycine, and 2,4-diaminobutyric acid. An additional
remarkable difference from other Agromyces species
is that MK-12 was the sole menaquinone in strain HY052T.
Based on results from the polyphasic characterizations performed
in this study, our isolates are proposed to be members
of a novel species in genus Agromyces, named Agromyces
laixinhei. The type strain is HY052T (= CGMCC 1.17175T =
JCM 33695T).
-
Citations
Citations to this article as recorded by - Validation List no. 223. Valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, Markus Göker
International Journal of Systematic and Evolutionary Microbiology
.2025;[Epub] CrossRef -
Agromyces archimandritae sp. nov., isolated from the cockroach Archimandrita tessellata
Juan Guzman, Maite Ortúzar, Anja Poehlein, Rolf Daniel, Martha E. Trujillo, Andreas Vilcinskas
International Journal of Systematic and Evolutionary Microbiology
.2022;[Epub] CrossRef - The Threat of Potentially Pathogenic Bacteria in the Feces of Bats
Yuyuan Huang, Yamin Sun, Qianni Huang, Xianglian Lv, Ji Pu, Wentao Zhu, Shan Lu, Dong Jin, Liyun Liu, Zhengli Shi, Jing Yang, Jianguo Xu, Biao He
Microbiology Spectrum.2022;[Epub] CrossRef - Morphological and genomic characteristics of two novel halotolerant actinomycetes, Tomitella gaofuii sp. nov. and Tomitella fengzijianii sp. nov. isolated from bat faeces
Yuyuan Huang, Jing Yang, Shan Lu, Xin-He Lai, Dong Jin, Juan Zhou, Sihui Zhang, Qianni Huang, Xianglian Lv, Wentao Zhu, Ji Pu, Ying Huang, Liyun Liu, Jianguo Xu
Systematic and Applied Microbiology.2022; 45(2): 126294. CrossRef - Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp. nov., a novel halotolerant actinomycete isolated from bat feces
Yuyuan Huang, Lingzhi Dong, Jian Gong, Jing Yang, Shan Lu, Xin-He Lai, Dong Jin, Qianni Huang, Ji Pu, Liyun Liu, Jianguo Xu
Journal of Microbiology.2022; 60(10): 977. CrossRef
- Vagococcus zengguangii sp. nov., isolated from yak faeces
-
Yajun Ge , Dong Jin , Xin-He Lai , Jing Yang , Shan Lu , Ying Huang , Han Zheng , Xiaoyan Zhang , Jianguo Xu
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J. Microbiol. 2021;59(1):1-9. Published online December 23, 2020
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DOI: https://doi.org/10.1007/s12275-021-0406-3
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323
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Two unknown Gram-stain-positive, catalase- and oxidasenegative,
non-motile, and coccus-shaped bacteria, designated
MN-17T and MN-09, were isolated from yaks faeces (Bos
grunniens) in the Qinghai-Tibet Plateau of China. 16S rRNA
gene sequence-based comparative analyses revealed that the
two strains were grouped within the genus Vagococcus, displaying
the highest similarity with Vagococcus xieshaowenii
CGMCC 1.16436T (98.6%) and Vagococcus elongatus CCUG
51432T (96.4%). Both strains grew optimally at 37°C and pH
7.0 in the presence of 0.5% (w/v) NaCl. The complete genome
of MN-17T comprises 2,085 putative genes with a total
of 2,190,262 bp and an average G + C content of 36.7 mol%.
The major fatty acids were C16:0 (31.2%), C14:0 (28.5%), and
C18:1ω9c (13.0%); the predominant respiratory quinone was
MK-7 (68.8%); the peptidoglycan type was A4α(L-Lys-DAsp);
and the major polar lipid was diphosphatidylglycerol.
Together, these supported the affiliation of strain MN-17T
to the genus Vagococcus. In silico DNA-DNA hybridization
and the average nucleotide identity values between MN-17T
and all recognized species in the genus were 21.6–26.1%
and 70.7–83.0%, respectively. MN-17T produced acid from
D-cellobiose, D-fructose, glycerol, D-glucose, N-acetyl-glucosamine,
gentiobiose, D-mannose, D-maltose, D-ribose, Dsaccharose,
salicin, D-trehalose, and D-xylose. These results
distinguished MN-17T and MN-09 from closely related species
in Vagococcus. Thus, we propose that strains MN-17T
and MN-09 represent a novel species in the genus Vagococcus,
with the name Vagococcus zengguangii sp. The type strain
is MN-17T (= CGMCC 1.16726T = GDMCC 1.1589T = JCM
33478T).
-
Citations
Citations to this article as recorded by -
Vagococcus proximus sp. nov. and Vagococcus intermedius sp. nov., originating from modified atmosphere packaged broiler meat
Per Johansson, Elina Jääskeläinen, Elina Säde, Johanna Björkroth
International Journal of Systematic and Evolutionary Microbiology
.2023;[Epub] CrossRef - Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp. nov., a novel halotolerant actinomycete isolated from bat feces
Yuyuan Huang, Lingzhi Dong, Jian Gong, Jing Yang, Shan Lu, Xin-He Lai, Dong Jin, Qianni Huang, Ji Pu, Liyun Liu, Jianguo Xu
Journal of Microbiology.2022; 60(10): 977. CrossRef
- Georgenia faecalis sp. nov. isolated from the faeces of Tibetan antelope
-
Xiaoxia Wang , Jing Yang , Yuyuan Huang , Xiaomin Wu , Licheng Wang , Limei Han , Sha Li , Huan Li , Xiaoying Fu , Hai Chen , Xiong Zhu
-
J. Microbiol. 2020;58(9):734-740. Published online July 24, 2020
-
DOI: https://doi.org/10.1007/s12275-020-0060-1
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Two aerobic, Gram-stain-positive, non-motile, non-sporulating
coccoid strains, designated ZLJ0423T and ZLJ0321,
were isolated from the faeces of Tibetan antelope (Pantholops
hodgsonii). Their optimal temperature, NaCl concentration
and pH for growth were 28°C, 0.5% (w/v) NaCl and pH 7.5,
respectively. Phylogenetic analysis based on 16S rRNA gene
sequences revealed that strains ZLJ0423T and ZLJ0321 were
very similar to each other (99.8%) and had a sequence similarity
of 97.0% with Georgenia satyanarayanai NBRC 107612T
and Georgenia subflava CGMCC 1.12782T. Phylogenomic
analysis based on 688 core genes indicated that these strains
formed a clade with G. satyanarayanai NBRC 107612T and
Georgenia wutianyii Z294T. The predominant cellular fatty
acids were anteiso-C15:0, anteiso-C15:1 A and C16:0. The major
menaquinone was MK-8(H4). The cell-wall amino acids consisted
of alanine, lysine, glycine and aspartic acid, with lysine
as the diagnostic diamino acid. Diphosphatidylglycerol,
phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol
mannosides and two unidentified lipids formed the
polar lipid profile. The DNA G + C content of both isolates
was 73.9 mol%. The digital DNA–DNA hybridization value
between strains ZLJ0423T and ZLJ0321 was 91.2%, but their
values with closely related species and other available type
strains of the genus Georgenia were lower than the 70% threshold.
On the basis of polyphasic taxonomic data, strains
ZLJ0423T and ZLJ0321 represent a novel species within the
genus Georgenia, for which the name Georgenia faecalis sp.
nov. is proposed. The type strain is ZLJ0423T (= CGMCC
1.13681T = JCM 33470T).
-
Citations
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Linpei Han, Lei Li, Yun Xu, Xinyi Xu, Wenjie Ye, Yuanji Kang, Feng Zhen, Xuya Peng
Waste Management.2024; 180: 135. CrossRef -
Georgenia halotolerans sp. nov., a halotolerant actinobacterium isolated from Taklamakan desert soil
Shao-Wei Liu, Ke-Ke Luo, Fei-Na Li, Ben-Yin Zhang, De-Jun Zhang, Cheng-Hang Sun
International Journal of Systematic and Evolutionary Microbiology
.2023;[Epub] CrossRef
- Streptococcus pneumoniae aminopeptidase N contributes to bacterial virulence and elicits a strong innate immune response through MAPK and PI3K/AKT signaling
-
Ling Wang , Xuemei Zhang , Guangying Wu , Yuhong Qi , Jinghui Zhang , Jing Yang , Hong Wang , Wenchun Xu
-
J. Microbiol. 2020;58(4):330-339. Published online February 27, 2020
-
DOI: https://doi.org/10.1007/s12275-020-9538-0
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337
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11
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10
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Abstract
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Streptococcus pneumoniae is a Gram-positive pathogen with
high morbidity and mortality globally but some of its pathogenesis
remains unknown. Previous research has provided
evidence that aminopeptidase N (PepN) is most likely a virulence
factor of S. pneumoniae. However, its role in S. pneumoniae
virulence and its interaction with the host remains
to be confirmed. We generated a pepN gene deficient mutant
strain and found that its virulence for mice was significantly
attenuated as were in vitro adhesion and invasion of host
cells. The PepN protein could induce a strong innate immune
response in vivo and in vitro and induced secretion of IL-6
and TNF-α by primary peritoneal macrophages via the rapid
phosphorylation of MAPK and PI3K/AKT signaling pathways
and this was confirmed using specific pathway inhibitors.
In conclusion, PepN is a novel virulence factor that is
essential for the virulence of S. pneumoniae and induces host
innate immunity via MAPK and PI3K/AKT signaling.
-
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