- Biosynthesis of adipic acid in metabolically engineered Saccharomyces cerevisiae
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Xi Zhang , Yingli Liu , Jing Wang , Yunying Zhao , Yu Deng
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J. Microbiol. 2020;58(12):1065-1075. Published online October 23, 2020
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DOI: https://doi.org/10.1007/s12275-020-0261-7
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103
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15
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 Abstract
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Adipic Acid (AA) is a valued platform chemical compound,
which can be used as a precursor of nylon-6,6. Due to the
generation of an enormous amount of nitric oxide metabolites
and the growing depletion of oil resources as a result of
AA production from a mixture of cyclohexanol and cyclohexanone,
the microbial methods for synthesizing AA have
attracted significant attention. Of the several AA-producing
pathways, the reverse adipate degradation pathway in
Thermobifida fusca (Tfu RADP) is reported to be the most
efficient, which has been confirmed in Escherichia coli. In this
study, the heterologous Tfu RADP was constructed for producing
AA in S. cerevisiae by co-expressing genes of Tfu_
0875, Tfu_2399, Tfu_0067, Tfu_1647, Tfu_2576, and Tfu_
2576. The AA titer combined with biomass, cofactors and
other by-products was all determined after fermentation.
During batch fermentation in a shake flask, the maximum AA
titer was 3.83 mg/L, while the titer increased to 10.09 mg/L
during fed-batch fermentation in a 5-L bioreactor after fermentation
modification.
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Citations
Citations to this article as recorded by  - Synthetic biology strategies for sustainable bioplastic production by yeasts
Huong-Giang Le, Yongjae Lee, Sun-Mi Lee
Journal of Microbiology.2025; 63(3): e2501022. CrossRef - Production of dicarboxylates from ω-amino acids using a cofactor- and co-substrate-free in vitro biosynthetic system
Jinxin Yan, Hui Zhang, Hongxu Zhang, Hairong Yu, Wenjia Tian, Mingyuan Liu, Weikang Sun, Leilei Guo, Xiaoxu Tan, Kaiyu Gao, Tianyi Jiang, Chuanjuan Lü, Qianjin Kang, Wensi Meng, Cuiqing Ma, Chao Gao, Ping Xu
Engineering Microbiology.2025; 5(3): 100210. CrossRef - Comparative Analysis of Biotechnological and Catalytic Approaches to Synthesizing Organic Acids
K. N. Sorokina, Yu. V. Samoylova, V. N. Parmon
Catalysis in Industry.2025; 17(1): 75. CrossRef - Experimental, modeling and optimisation of adipic acid reactive extraction using ionic liquids
Elena Niculina Dragoi, Alexandra Cristina Blaga, Dan Cascaval, Anca Irina Galaction
Journal of Molecular Liquids.2024; 410: 125564. CrossRef - Structure sensitivity of the electrochemical hydrogenation of cis,cis-muconic acid to hexenedioic acid and adipic acid
Deep M. Patel, Prathamesh T. Prabhu, Geet Gupta, Marco Nazareno Dell'Anna, Samantha Kling, Huy T. Nguyen, Jean-Philippe Tessonnier, Luke T. Roling
Green Chemistry.2024; 26(8): 4506. CrossRef - Exploring the Potential of Bio-plasticizers: Functions, Advantages, and Challenges in Polymer Science
Felipe Martins de Souza, Ram K. Gupta
Journal of Polymers and the Environment.2024; 32(11): 5499. CrossRef - Poly (Butylene Adipate‐Co‐Terephthalate) (PBAT) – Based Biocomposites: A Comprehensive Review
Blessing E. Itabana, Amar K. Mohanty, Phil Dick, Mohini Sain, Atul Bali, Mike Tiessen, Loong‐Tak Lim, Manjusri Misra
Macromolecular Materials and Engineering.2024;[Epub] CrossRef - Comparative analysis of biotechnological and catalytic approaches to the production of organic acids
K. N. Sorokina, Y. V. Samoylova, V. N. Parmon
Kataliz v promyshlennosti.2024; 24(3): 73. CrossRef - Mid–Long Chain Dicarboxylic Acid Production via Systems Metabolic Engineering: Progress and Prospects
Shanna Gu, Fuzhou Zhu, Lin Zhang, Jianping Wen
Journal of Agricultural and Food Chemistry.2024; 72(11): 5555. CrossRef - Toward the use of mixed microbial cultures for the biological production of adipic and levulinic acid
Fernanda Pinto-Ibieta, Mara Cea, Antonio Serrano, Fernando E. Felissia, María Cristina Area, Francisco Cabrera, Gustavo Ciudad
Frontiers in Microbiology.2023;[Epub] CrossRef - Producing malonate in Saccharomyces cerevisiae via the β-alanine pathway
Shiyun Li, Wenxuan Fu, Ruifang Su, Yunying Zhao, Yu Deng
Systems Microbiology and Biomanufacturing.2023; 3(2): 328. CrossRef - Engineering yeast cell factories to produce biodegradable plastics and their monomers: Current status and prospects
Feng-Li Zhang, Lin Zhang, Du-Wen Zeng, Sha Liao, Yachao Fan, Verawat Champreda, Weerawat Runguphan, Xin-Qing Zhao
Biotechnology Advances.2023; 68: 108222. CrossRef - Sustainable Routes for the Synthesis of Renewable Adipic Acid from Biomass Derivatives
Man Lang, Hao Li
ChemSusChem.2022;[Epub] CrossRef - Research Progress on the Construction of Artificial Pathways for the Biosynthesis of Adipic Acid by Engineered Microbes
Yuchen Ning, Huan Liu, Renwei Zhang, Yuhan Jin, Yue Yu, Li Deng, Fang Wang
Fermentation.2022; 8(8): 393. CrossRef - Opportunities in the microbial valorization of sugar industrial organic waste to biodegradable smart food packaging materials
Sandhya Jayasekara, Lakshika Dissanayake, Lahiru N. Jayakody
International Journal of Food Microbiology.2022; 377: 109785. CrossRef - Implementation of Synthetic Pathways to Foster Microbe-Based Production of Non-Naturally Occurring Carboxylic Acids and Derivatives
Ana Vila-Santa, Fernão C. Mendes, Frederico C. Ferreira, Kristala L. J. Prather, Nuno P. Mira
Journal of Fungi.2021; 7(12): 1020. CrossRef
- Chitosan-chelated zinc modulates cecal microbiota and attenuates inflammatory response in weaned rats challenged with Escherichia coli
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Dan Feng , Minyang Zhang , Shiyi Tian , Jing Wang , Weiyun Zhu
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J. Microbiol. 2020;58(9):780-792. Published online September 1, 2020
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DOI: https://doi.org/10.1007/s12275-020-0056-x
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76
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10
Web of Science
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9
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Abstract
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Escherichia coli (E. coli) infection is very common among
young growing animals, and zinc supplementation is often
used to alleviate inflammation induced by this disease. Therefore,
the objective of this study was to evaluate whether chitosan-
chelated zinc (CS-Zn) supplementation could attenuate
gut injury induced by E. coli challenge and to explore how CSZn
modulates cecal microbiota and alleviates intestinal inflammation
in weaned rats challenged with E. coli. 36 weaned
rats (55.65 ± 2.18 g of BW, n = 12) were divided into three
treatment groups consisting of unchallenged rats fed a basal
diet (Control) and two groups of rats challenged with E. coli
and fed a basal diet or a diet containing 640 mg/kg CS-Zn
(E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
On days 10 to 12, each rat was given 4 ml of E. coli
solution with a total bacteria count of 1010 CFU by oral gavage
daily or normal saline of equal dosage. CS-Zn supplementation
mitigated intestinal morphology impairment (e.g.
higher crypt depth and lower macroscopic damage index)
induced by E. coli challenge (P < 0.05), and alleviated the increase
of Myeloperoxidase (MPO) activity after E. coli challenge
(P < 0.05). 16S rRNA sequencing analyses revealed that
E. coli challenge significantly increased the abundance of Verrucomicrobia
and E. coli (P < 0.05). However, CS-Zn supplementation
increased the abundance of Lactobacillus and decreased
the relative abundance of Proteobacteria, Desulfovibrio
and E. coli (P < 0.05). The concentrations of butyrate in
the cecal digesta, which decreased due to the challenge, were
higher in the E. coli + CS-Zn group (P < 0.05). In addition,
CS-Zn supplementation significantly prevented the elevation
of pro-inflammatory cytokines IL-6 concentration and upregulated
the level of anti-inflammatory cytokines IL-10 in
cecal mucosa induced by E. coli infection (P < 0.05). In conclusion,
these results indicate that CS-Zn produces beneficial
effects in alleviating gut mucosal injury of E. coli challenged
rats by enhancing the intestinal morphology and modulating
cecal bacterial composition, as well as attenuating inflammatory
response.
-
Citations
Citations to this article as recorded by - Organic zinc glycine chelate is better than inorganic zinc in improving growth performance of cherry valley ducks by regulating intestinal morphology, barrier function, and the gut microbiome
Yaqi Chang, Ke Wang, Min Wen, Bing Wu, Guangmang Liu, Hua Zhao, Xiaoling Chen, Jingyi Cai, Gang Jia
Journal of Animal Science.2023;[Epub] CrossRef - Effects on the intestinal morphology, inflammatory response and microflora in piglets challenged with enterotoxigenic Escherichia coli K88
Kun Zhang, Xiaoyang Shen, Lu Han, Mengyun Wang, Shaoqiang Lian, Kejun Wang, Chunli Li
Research in Veterinary Science.2023; 157: 50. CrossRef - Surface engineering of chitosan nanosystems and the impact of functionalized groups on the permeability of model drug across intestinal tissue
Sadaf Ejaz, Syed Muhammad Afroz Ali, Bina Zarif, Ramla Shahid, Ayesha Ihsan, Tayyaba Noor, Muhammad Imran
International Journal of Biological Macromolecules.2023; 242: 124777. CrossRef - Chitosan Protects Immunosuppressed Mice Against Cryptosporidium parvum Infection Through TLR4/STAT1 Signaling Pathways and Gut Microbiota Modulation
Sajid Ur Rahman, Haiyan Gong, Rongsheng Mi, Yan Huang, Xiangan Han, Zhaoguo Chen
Frontiers in Immunology.2022;[Epub] CrossRef - Effect of Dietary Zinc Methionine Supplementation on Growth Performance, Immune Function and Intestinal Health of Cherry Valley Ducks Challenged With Avian Pathogenic Escherichia coli
Yaqi Chang, Jia Mei, Ting Yang, Zhenyu Zhang, Guangmang Liu, Hua Zhao, Xiaoling Chen, Gang Tian, Jingyi Cai, Bing Wu, Fali Wu, Gang Jia
Frontiers in Microbiology.2022;[Epub] CrossRef - Chitosan-chelated zinc modulates ileal microbiota, ileal microbial metabolites, and intestinal function in weaned piglets challenged with Escherichia coli K88
Guojun Hou, Minyang Zhang, Jing Wang, Weiyun Zhu
Applied Microbiology and Biotechnology.2021; 105(19): 7529. CrossRef - Gut Microbiota as a Mediator of Essential and Toxic Effects of Zinc in the Intestines and Other Tissues
Anatoly V. Skalny, Michael Aschner, Xin Gen Lei, Viktor A. Gritsenko, Abel Santamaria, Svetlana I. Alekseenko, Nagaraja Tejo Prakash, Jung-Su Chang, Elena A. Sizova, Jane C. J. Chao, Jan Aaseth, Alexey A. Tinkov
International Journal of Molecular Sciences.2021; 22(23): 13074. CrossRef - Potential Applications of Chitosan-Based Nanomaterials to Surpass the Gastrointestinal Physiological Obstacles and Enhance the Intestinal Drug Absorption
Nutthapoom Pathomthongtaweechai, Chatchai Muanprasat
Pharmaceutics.2021; 13(6): 887. CrossRef - Modulation of Gut Microbiota for the Prevention and Treatment of COVID-19
Jiezhong Chen, Luis Vitetta
Journal of Clinical Medicine.2021; 10(13): 2903. CrossRef
- Epidemiology and resistance features of Acinetobacter baumannii isolates from the ward environment and patients in the burn ICU of a Chinese hospital
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Yali Gong , Xiaodong Shen , Guangtao Huang , Cheng Zhang , Xiaoqiang Luo , Supeng Yin , Jing Wang , Fuquan Hu , Yizhi Peng , Ming Li
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J. Microbiol. 2016;54(8):551-558. Published online August 2, 2016
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DOI: https://doi.org/10.1007/s12275-016-6146-0
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79
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22
Crossref
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Abstract
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Acinetobacter baumannii is an important opportunistic pathogen
that causes severe nosocomial infections, especially
in intensive care units (ICUs). Over the past decades, an everincreasing
number of hospital outbreaks caused by A. baumannii
have been reported worldwide. However, little attention
has been directed toward the relationship between A. baumannii
isolates from the ward environment and patients in
the burn ICU. In this study, 88 A. baumannii isolates (26 from
the ward environment and 62 from patients) were collected
from the burn ICU of the Southwest Hospital in Chongqing,
China, from July through December 2013. Antimicrobial susceptibility
testing results showed that drug resistance was more
severe in isolates from patients than from the ward environment,
with all of the patient isolates being fully resistant to
10 out of 19 antimicrobials tested. Isolations from both the
ward environment and patients possessed the β-lactamase
genes blaOXA-51, blaOXA-23, blaAmpC, blaVIM, and blaPER. Using
pulsed-field gel electrophoresis (PFGE) and multi-locus sequence
typing (MLST), these isolates could be clustered into
4 major PFGE types and 4 main sequence types (ST368, ST369,
ST195, and ST191) among which, ST368 was the dominant
genotype. Epidemiologic and molecular typing data also revealed
that a small-scale outbreak of A. baumannii infection
was underway in the burn ICU of our hospital during the
sampling period. These results suggest that dissemination
of β-lactamase genes in the burn ICU might be closely associated
with the high-level resistance of A. baumannii, and
the ICU environment places these patients at a high risk for
nosocomial infection. Cross-contamination should be an
important concern in clinical activities to reduce hospital acquired infections caused by A. baumannii.
-
Citations
Citations to this article as recorded by - Prevalence, Characterization, and Epidemiological Relationships between ESBL and Carbapenemase-Producing Escherichia coli, Klebsiella pneumoniae, and Acinetobacter spp. Isolated from Humans and the Kitchen Environment of Two Greek Hospitals
Anestis Tsitsos, Alexandros Damianos, Maria Boutel, Panagiota Gousia, Nikolaos Soultos, Anna Papa, Ilias Tirodimos, Vangelis Economou
Antibiotics.2024; 13(10): 934. CrossRef - Molecular Typing and Resistance Profile of Acinetobacter baumannii Isolates during the COVID-19 Pandemic: Findings from the “EPIRADIOCLINF” Project
Antonella Agodi, Arturo Montineri, Rosa Manuele, Paola Noto, Giuseppe Carpinteri, Giacomo Castiglione, Patrizia Grassi, Antonio Lazzara, Anna Rita Mattaliano, Giuseppa Granvillano, Claudia La Mastra, Maria Clara La Rosa, Andrea Maugeri, Martina Barchitta
Antibiotics.2023; 12(10): 1551. CrossRef - Dissemination and Genetic Relatedness of Multidrug-Resistant and Extensively Drug-Resistant Acinetobacter baumannii Isolates from a Burn Hospital in Iraq
Aras A. K Shali, Paywast J Jalal, Sehand K Arif, Abdelaziz Ed-dra
Canadian Journal of Infectious Diseases and Medical Microbiology.2022; 2022: 1. CrossRef - Acinetobacter baumannii Isolates from COVID-19 Patients in a Hospital Intensive Care Unit: Molecular Typing and Risk Factors
Mariateresa Ceparano, Valentina Baccolini, Giuseppe Migliara, Claudia Isonne, Erika Renzi, Daniela Tufi, Corrado De Vito, Maria De Giusti, Maria Trancassini, Francesco Alessandri, Giancarlo Ceccarelli, Francesco Pugliese, Paolo Villari, Maria Angiulli, St
Microorganisms.2022; 10(4): 722. CrossRef - The characteristic and potential therapeutic effect of isolated multidrug-resistant Acinetobacter baumannii lytic phage
Behnam Sisakhtpour, Arezoo Mirzaei, Vajihe Karbasizadeh, Nafiseh Hosseini, Mehdi Shabani, Sharareh Moghim
Annals of Clinical Microbiology and Antimicrobials.2022;[Epub] CrossRef - The Relationship between Carbapenem Resistance and Biofilm Formation in Clinical Acinetobacter Baumannii Isolates
Fariba Naeimi Mazraeh, Naser Alizadeh, Roghayeh Nouri, Hossein Samadi Kafil, Shahram Abdoli Oskouie, Mohammad Ahangarzadeh Rezaee
SSRN Electronic Journal .2022;[Epub] CrossRef - The Low-Alkalinity Polymyxin Derivative, AL-6, Shows High Activity Against Multidrug-Resistant Acinetobacter baumannii Clinical Isolates In Vitro and A. baumannii ATCC 19606 In Vivo: Preliminary Analysis of the Antibacterial Mechanism
Dai-Jie Chen, A-Long Cui, Jia-Rong Chen, Ping Yang, Jie Jin, Lei Shao, Zhuo-Rong Li
Microbial Drug Resistance.2021; 27(7): 933. CrossRef - Different Infection Profiles and Antimicrobial Resistance Patterns Between Burn ICU and Common Wards
Yali Gong, Yuan Peng, Xiaoqiang Luo, Cheng Zhang, Yunlong Shi, Yixin Zhang, Jun Deng, Yizhi Peng, Gaoxing Luo, Haisheng Li
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef - Epidemiological Analysis of Multidrug-Resistant Acinetobacter baumannii Isolates in a Tertiary Hospital Over a 12-Year Period in China
Meijie Jiang, Xia Chen, Shuang Liu, Zhijun Zhang, Ning Li, Chao Dong, Ling Zhang, Haiyan Wu, Shuping Zhao
Frontiers in Public Health.2021;[Epub] CrossRef - Pathogenic Characteristics and Risk Factors for ESKAPE Pathogens Infection in Burn Patients
Zhaoyinqian Li, Jingling Xie, Jiaxin Yang, Siyi Liu, Zixuan Ding, Jingchen Hao, Yinhuan Ding, Zhangrui Zeng, Jinbo Liu
Infection and Drug Resistance.2021; Volume 14: 4727. CrossRef - High frequency of blaPER-1 gene in clinical strains of Acinetobacter baumannii and its association with quorum sensing and virulence factors
Fariba Naeimi Mazraeh, Alka Hasani, Javid Sadeghi, Hossein Samadi Kafil, Mohammad Hossein Soroush Barhaghi, Fatemeh Yeganeh Sefidan, Hamideh Rishi Sharabiani, Yalda Hematyar, Mohammad Ahangarzadeh Rezaee
Gene Reports.2021; 24: 101232. CrossRef - Isolation and Characterization of a Novel Myophage Abp9 Against Pandrug Resistant Acinetobacater baumannii
Lingli Jiang, Jingjie Tan, Yi Hao, Qi Wang, Xiaorui Yan, Dali Wang, Li Tuo, Zairong Wei, Guangtao Huang
Frontiers in Microbiology.2020;[Epub] CrossRef - Molecular typing of multi-drug resistant Acinetobacter baumannii isolates from clinical and environmental specimens in three Iranian hospitals by pulsed field gel electrophoresis
Ali Mohammadi Bardbari, Parviz Mohajeri, Mohammad Reza Arabestani, Manoochehr Karami, Fariba Keramat, Saba Asadollahi, Amir Khodavirdipour, Mohammad Yousef Alikhani
BMC Microbiology.2020;[Epub] CrossRef - The impact of serum protein binding on bacterial killing of minocycline
Li-Xia Xie, Jian Zhou, Kimberly R. Ledesma, Paul R. Merlau, Vincent H. Tam
Journal of Global Antimicrobial Resistance.2020; 21: 252. CrossRef - The distribution of mutations and hotspots in transcription regulators of resistance-nodulation-cell division efflux pumps in tigecycline non-susceptible Acinetobacter baumannii in China
Qingye Xu, Xiaoting Hua, Jintao He, Di Zhang, Qiong Chen, Linghong Zhang, Belinda Loh, Sebastian Leptihn, Yurong Wen, Paul G. Higgins, Yunsong Yu, Zhihui Zhou
International Journal of Medical Microbiology.2020; 310(8): 151464. CrossRef - In vitro activities of Eravacycline against 336 isolates collected from 2012 to 2016 from 11 teaching hospitals in China
Chunjiang Zhao, Xiaojuan Wang, Yawei Zhang, Ruobing Wang, Qi Wang, Henan Li, Hui Wang
BMC Infectious Diseases.2019;[Epub] CrossRef - Carbapenem-resistant Acinetobacter baumannii in patients with burn injury: A systematic review and meta-analysis
William Gustavo Lima, Geisa Cristina Silva Alves, Cristina Sanches, Simone Odília Antunes Fernandes, Magna Cristina de Paiva
Burns.2019; 45(7): 1495. CrossRef - Global Transcriptomic Analysis of the Interactions between Phage φAbp1 and Extensively Drug-Resistant Acinetobacter baumannii
Zichen Yang, Supeng Yin, Gang Li, Jing Wang, Guangtao Huang, Bei Jiang, Bo You, Yali Gong, Cheng Zhang, Xiaoqiang Luo, Yizhi Peng, Xia Zhao, Sergio Baranzini
mSystems.2019;[Epub] CrossRef - Environmental contamination by carbapenem-resistant Acinetobacter baumannii: The effects of room type and cleaning methods
Anat Or Lerner, Jalal Abu-Hanna, Yehuda Carmeli, Vered Schechner
Infection Control & Hospital Epidemiology.2019; : 1. CrossRef - Janus N,N-dimethylformamide as a solvent for a gradient porous wound dressing of poly(vinylidene fluoride) and as a reducer for in situ nano-silver production: anti-permeation, antibacterial and antifouling activities against multi-drug-resistant bacteria
Menglong Liu, Ying Wang, Xiaodong Hu, Weifeng He, Yali Gong, Xiaohong Hu, Meixi Liu, Gaoxing Luo, Malcolm Xing, Jun Wu
RSC Advances.2018; 8(47): 26626. CrossRef - Whole-Genome Analysis of an Extensively Drug-Resistant Acinetobacter baumannii Strain XDR-BJ83: Insights into the Mechanisms of Resistance of an ST368 Strain from a Tertiary Care Hospital in China
Yang-Yang Zhang, Zhi-Xin Liang, Chun-Sun Li, Yan Chang, Xiu-Qing Ma, Ling Yu, Liang-An Chen
Microbial Drug Resistance.2018; 24(9): 1259. CrossRef - Multilocus sequence typing and molecular characterization of β-lactamase genes among Acinetobacter baumannii isolates in a burn center
Guangtao Huang, Yuan Peng, Yong Yang, Chengyong Tang, Yuexian Fu
Burns.2017; 43(7): 1473. CrossRef
- Effects of Exopolysaccharide Production on Liquid Vegetative Growth, Stress Survival, and Stationary Phase Recovery in Myxococcus xanthus
-
Wei Hu , Jing Wang , Ian McHardy , Renate Lux , Zhe Yang , Yuezhong Li , Wenyuan Shi
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J. Microbiol. 2012;50(2):241-248. Published online April 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1349-5
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52
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11
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Abstract
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Exopolysaccharide (EPS) of Myxococcus xanthus is a wellregulated
cell surface component. In addition to its known
functions for social motility and fruiting body formation
on solid surfaces, EPS has also been proposed to play a role
in multi-cellular clumping in liquid medium, though this
phenomenon has not been well studied. In this report, we
confirmed that M. xanthus clumps formed in liquid were
correlated with EPS levels and demonstrated that the EPS
encased cell clumps exhibited biofilm-like structures. The
clumps protected the cells at physiologically relevant EPS
concentrations, while cells lacking EPS exhibited significant
reduction in long-term viability and resistance to stressful
conditions. However, excess EPS production was counterproductive
to vegetative growth and viable cell recovery declined
in extended late stationary phase as cells became
trapped in the matrix of clumps. Therefore, optimal EPS
production by M. xanthus is important for normal physiological
functions in liquid.
- Functional Definition of LuxS, an Autoinducer-2 (AI-2) Synthase and Its Role in Full Virulence of Streptococcus suis Serotype 2
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Min Cao , Youjun Feng , Changjun Wang , Feng Zheng , Ming Li , Hui Liao , Yinghua Mao , Xiuzhen Pan , Jing Wang , Dan Hu , Fuquan Hu , Jiaqi Tang
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J. Microbiol. 2011;49(6):1000-1011. Published online December 28, 2011
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DOI: https://doi.org/10.1007/s12275-011-1523-1
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45
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34
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Abstract
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Quorum sensing is a widespread chemical communication in response to fluctuation of bacterial population density, and has been implicated into bacterial biofilm formation and regulation of expression of virulence factors. The luxS gene product, S-ribosylhomocysteinase, catalizes the last committed step in biosynthetic pathway of autoinducer 2 (AI-2), a signaling molecule for inter-species quorum sensing. We found a luxS homologue in 05ZYH33, an epidemic strain of Streptococcus suis serotype 2 (SS2) in China. A luxS null mutant (ΔluxS) of 05ZYH33 strain was obtained using an approach of homologous recombination. LuxS was determined to be required for AI-2 production in 05ZYH33 strain of S. suis 2. Inactivation of luxS gene led to a wide range of phenotypic changes including thinner capsular walls, increased tolerance to H2O2, reduced adherence capacity to epithelial cells, etc. In particular, loss of LuxS impaired dramatically its full virulence of SS2 in experimental model of piglets, and functional complementation restored it nearly to the level of parent strain. Genome-wide transcriptome analyses suggested that some known virulence factors such as CPS are down-regulated in the ΔluxS mutant, which might in part explain virulence attenuation by luxS deletion. Similarly, 29 of 71 genes with different expression level were proposed to be targets candidate regulated by LuxS/AI-2-dependent quorum sensing.
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Effects of vitamin B
12
supply on cellular processes of the facultative vitamin B
12
consumer
Vibrio campbellii
Luna-Agrippina Groon, Stefan Bruns, Leon Dlugosch, Heinz Wilkes, Gerrit Wienhausen, Isaac Cann
Applied and Environmental Microbiology.2025;[Epub] CrossRef - Cooperation of quorum sensing and central carbon metabolism in the pathogenesis of Gram-positive bacteria
Shuji Gao, Yuxin Wang, Shuo Yuan, Jing Zuo, Wenjie Jin, Yamin Shen, Daniel Grenier, Li Yi, Yang Wang
Microbiological Research.2024; 282: 127655. CrossRef - Collateral sensitivity to gamithromycin in ciprofloxacin-resistant Streptococcus suis is driven by increasing intracellular antibiotic accumulation
Yuejun Zhang, Mengting Tao, Ruiling Wang, Zilin Shi, Peipei Li, Sijia Tang, Jian Sun, Xiaoping Liao, Yufeng Zhou
Journal of Integrative Agriculture.2024;[Epub] CrossRef - Characterization of AI-2/LuxS quorum sensing system in biofilm formation, pathogenesis of Streptococcus equi subsp. zooepidemicus
Honglin Xie, Riteng Zhang, Ruhai Guo, Yining Zhang, Jingya Zhang, Hui Li, Qiang Fu, Xinglong Wang
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef - Whole-Genome Sequencing and Phenotypic Analysis of Streptococcus equi subsp. zooepidemicus Sequence Type 147 Isolated from China
Yan Su, Zehua Zhang, Li Wang, Baojiang Zhang, Lingling Su
Microorganisms.2024; 12(4): 824. CrossRef - A critical review on experimental Streptococcus suis infection in pigs with a focus on clinical monitoring and refinement strategies
Carolin Liedel, Karoline Rieckmann, Christoph G. Baums
BMC Veterinary Research.2023;[Epub] CrossRef - The Antibacterial Effect of Cannabigerol toward Streptococcus mutans Is Influenced by the Autoinducers 21-CSP and AI-2
Muna Aqawi, Ronit Vogt Sionov, Michael Friedman, Doron Steinberg
Biomedicines.2023; 11(3): 668. CrossRef - Contribution of quorum sensing to virulence and antibiotic resistance in zoonotic bacteria
Qingying Fan, Jing Zuo, Haikun Wang, Daniel Grenier, Li Yi, Yang Wang
Biotechnology Advances.2022; 59: 107965. CrossRef - mRNA-Seq reveals the quorum sensing system luxS gene contributes to the environmental fitness of Streptococcus suis type 2
Jinpeng Li, Yuxin Wang, Yanbin Du, Hui Zhang, Qingying Fan, Liyun Sun, Li Yi, Shaohui Wang, Yang Wang
BMC Microbiology.2021;[Epub] CrossRef - LsrR, the effector of AI-2 quorum sensing, is vital for the H2O2 stress response in mammary pathogenic Escherichia coli
Hui Wang, Fei Shang, Jiawei Shen, Jingyi Xu, Xiaolin Chen, Jingtian Ni, Lumin Yu, Ting Xue
Veterinary Research.2021;[Epub] CrossRef - Effect of Quorum Sensing Inhibitor Agents against Pseudomonas aeruginosa
M. Asif, M. Imran
Russian Journal of Bioorganic Chemistry.2020; 46(2): 149. CrossRef - Involvement of Various Enzymes in the Physiology and Pathogenesis of Streptococcus suis
Chengkun Zheng, Man Wei, Mengdie Jia, ManMan Cao
Veterinary Sciences.2020; 7(4): 143. CrossRef - How Streptococcus suis serotype 2 attempts to avoid attack by host immune defenses
Xiaojing Xia, Wanhai Qin, Huili Zhu, Xin Wang, Jinqing Jiang, Jianhe Hu
Journal of Microbiology, Immunology and Infection.2019; 52(4): 516. CrossRef - Quorum Sensing Inhibition: Current Advances of the Natural Antimicrobial Agents
M. Asif, M. Imran
Russian Journal of Bioorganic Chemistry.2019; 45(6): 488. CrossRef - Does the Endocannabinoid Anandamide Affect Bacterial Quorum Sensing, Vitality, and Motility?
Liat Friedman, Reem Smoum, Mark Feldman, Raphael Mechoulam, Doron Steinberg
Cannabis and Cannabinoid Research.2019; 4(2): 102. CrossRef - The LuxS/AI-2 system of Streptococcus suis
Yang Wang, Yuxin Wang, Liyun Sun, Daniel Grenier, Li Yi
Applied Microbiology and Biotechnology.2018; 102(17): 7231. CrossRef - Role of two-component regulatory systems in the virulence of Streptococcus suis
Chengkun Zheng, Lingzhi Li, Haojie Ge, Hongmei Meng, Yang Li, Weicheng Bei, Xiaohui Zhou
Microbiological Research.2018; 214: 123. CrossRef - Critical Streptococcus suis Virulence Factors: Are They All Really Critical?
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- The Use of Pseudomonas fluorescens P13 to Control Sclerotinia Stem Rot (Sclerotinia sclerotiorum) of Oilseed Rape
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Hui Li , Huaibo Li , Yan Bai , Jing Wang , Ming Nie , Bo Li , Ming Xiao
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J. Microbiol. 2011;49(6):884-889. Published online December 28, 2011
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DOI: https://doi.org/10.1007/s12275-011-1261-4
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Abstract
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Sclerotinia stem rot (SSR) caused by the fungus Sclerotinia sclerotiorum has been an increasing threat to oilseed rape (Brassica napus L.) cultivation. Efficient and environment‐friendly treatments are much needed. Here we focus on microbial control. The Pseudomonas fluorescens P13 that was isolated from oilseed rape cultivation soil, proved to be a useful biocontrol strain for application. Morphology, physiological and biochemical tests and 16S rDNA analysis demonstrated that it was P. fluorescens P13 and that it had a broad antagonistic spectrum, significantly lessening the mycelial growth of S. sclerotiorum by 84.4% and suppressing sclerotial formation by 95‐100%. Scanning electron microscopy studies attested that P13 deformed S. sclerotiorum mycelia when they were cultured together. P13 did not produce chitinase but did produce hydrogen cyanide (HCN) which was likely one of the antagonistic mechanisms. The density of P13 remained at a high level (≥106 CFU/ml) during 5 weeks in the rhizosphere soil and roots. P13 reduced SSR severity at least by 59% in field studies and also promoted seedling growth (p<0.05) at the seedling stage. From these data, our work provided evidence that P13 could be a good alternative biological resource for biocontrol of S. sclerotiorum.
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