- Leucobacter coleopterorum sp. nov., Leucobacter insecticola sp. nov., and Leucobacter viscericola sp. nov., isolated from the intestine of the diving beetles, Cybister brevis and Cybister lewisianus, and emended description of the genus Leucobacter
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Dong-Wook Hyun , Hojun Sung , Pil Soo Kim , Ji-Hyun Yun , Jin-Woo Bae
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J. Microbiol. 2021;59(4):360-368. Published online January 26, 2021
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DOI: https://doi.org/10.1007/s12275-021-0472-6
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 Abstract
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Three novel bacterial strains, HDW9AT, HDW9BT, and
HDW9CT, isolated from the intestine of the diving beetles
Cybister lewisianus and Cybister brevis, were characterized as
three novel species using a polyphasic approach. The isolates
were Gram-staining-positive, strictly aerobic, non-motile,
and rod-shaped. They grew optimally at 30°C (pH 7) in the
presence of 0.5% (wt/vol) NaCl. Phylogenetic analysis based
on the 16S rRNA gene sequences revealed that they belong
to the genus Leucobacter and are closely related to L. denitrificans
M1T8B10T (98.4–98.7% sequence similarity). Average
nucleotide identity (ANI) values among the isolates were
76.4–84.1%. ANI values for the isolates and the closest taxonomic
species, L. denitrificans KACC 14055T, were 72.3–73.1%.
The isolates showed ANI values of < 76.5% with all analyzable
Leucobacter strains in the EzBioCloud database. The
genomic DNA G + C content of the isolates was 60.3–62.5%.
The polar lipid components were phosphatidylglycerol, diphosphatidylglycerol,
and other unidentified glycolipids, phospholipids,
and lipids. The major cellular fatty acids were anteiso-
C15:0, iso-C16:0, and anteiso-C17:0. MK-10 was the major
respiratory quinone, and MK-7 and MK-11 were the minor
respiratory quinones. The whole-cell sugar components of the
isolates were ribose, glucose, galactose, and mannose. The
isolates harbored L-2,4-diaminobutyric acid, L-serine, L-lysine,
L-aspartic acid, glycine, and D-glutamic acid within the
cell wall peptidoglycan. Based on phylogenetic, phenotypic,
chemotaxonomic, and genotypic analyses, strains HDW9AT,
HDW9BT, and HDW9CT represent three novel species within
the genus Leucobacter. We propose the name Leucobacter
coleopterorum sp. nov. for strain HDW9AT (= KACC 21331T
= KCTC 49317T = JCM 33667T), the name Leucobacter insecticola
sp. nov. for strain HDW9BT (= KACC 21332T =
KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola
sp. nov. for strain HDW9CT (= KACC 21333T =
KCTC 49319T = JCM 33669T).
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Citations
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- Acinetobacter apis sp. nov., Isolated from the Intestinal Tract of a Honey Bee, Apis mellifera
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Pil Soo Kim , Na-Ri Shin , Joon Yong Kim , Ji-Hyun Yun , Dong-Wook Hyun , Jin-Woo Bae
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J. Microbiol. 2014;52(8):639-645. Published online August 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-4078-0
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87
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Abstract
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A novel Gram-negative, obligate aerobic, non-motile, and both coccobacillus- and bacillus-shaped bacterium, designated strain HYN18T, was isolated from the intestinal tract of a honey bee (Apis mellifera). The isolate was oxidasenegative
and catalase-positive. Strain HYN18T showed optimum growth at 25°C, pH 6–7, and in the presence of 1% (w/v) NaCl in trypticase soy broth medium. The isolate was
negative for hydrolyses of starch, casein, gelatin and urea, indole production from tryptone and hemolysis on sheep blood agar. A phylogenetic analysis based on the 16S rRNA gene and rpoB gene sequence showed that strain HYN18T was most closely related to Acinetobacter nectaris SAP 763.2T and A. boissieri SAP 284.1T with 98.3% and 98.1% similarity (16S rRNA gene), respectively, and 84.4% similarity with Acinetobacter nectaris SAP 763.2T (rpoB gene). The major cellular fatty acids were summed features 3 (comprising C16:1ω7c/C16:1ω6c), C12:0 and C16:0. The main isoprenoid quinone was ubiquinone-9 (Q-9). The polar lipids of strain
HYN18T were phosphatidylethanolamine, three unidentified lipids, an unidentified phospholipid and an unidentified glycolipid. The DNA G+C content was 40.6 mol%. DNADNA hybridization experiments indicated less than 33 ± 10% relatedness to the closest phylogenetic species, Acinetobacter nectaris SAP 763.2T. Thus, the phenotypic, phylogenetic and genotypic analyses indicate that strain HYN18T is a novel species within the genus Acinetobacter, for which the name Acinetobacter apis is proposed. The type strain is HYN18T (=KACC 16906T =JCM 18575T).
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- Molecular Cloning of the Superoxide Dismutase Gene from Orientia tsutsugamushi, the Causative Agent of Scrub Typhus
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Ji-Hyun Yun , Young-Sang Koh , Se-Jae Kim
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J. Microbiol. 2002;40(2):151-155.
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Abstract
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A superoxide dismutase (SOD) gene from the obligate intracellular bacterium Orientia tsutsugamushi has been cloned by using the polymerase chain reaction with degenerate oligonucleotide primers corresponding to conserved regions of known SODs. Nucleotide sequencing revealed that the predicted amino acid sequence was significantly more homologous to known iron-containing SODs (FeSOD) than to manganese-containing SODs (MnSOD). Conserved regions in bacterial FeSOD could also be seen. Isolation of the oriential SOD gene may provide an opportunity to examine its role in the intracellular survival of this bacterium.
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