- Microbiome therapeutic PMC72 through reverse translational research in gout
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Mohammed Solayman Hossain, Hoonhee Seo, Kyung-Ann Lee, Asad ul-Haq, Sukyung Kim, Sujin Jo, Md Abdur Rahim, Hanieh Tajdozian, Fatemeh Ghorbanian, Youjin Yoon, Indrajeet Barman, Md Sarower Hossen Shuvo, Hyun-Sook Kim, Ho-Yeon Song
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J. Microbiol. 2025;63(5):e2501002. Published online May 27, 2025
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DOI: https://doi.org/10.71150/jm.2501002
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Gout is an inflammatory arthritis resulting from the deposition of monosodium urate crystals. Urate-lowering therapies for gout have limitations, including side effects and limited efficacy, highlighting the need for novel therapeutic approaches to improve patient outcomes. In this context, our research team conducted a microbiome analysis of fecal samples from healthy individuals and gout patients, identifying Bifidobacterium as a key biomarker. Subsequently, we isolated and identified this strain, B. longum PMC72, and demonstrated its efficacy in a gout mouse model. In potassium oxonate (PO)-induced hyperuricemia mice, PMC72 significantly alleviated nausea, gait disturbances, ankle inflammation, and improved renal health. These effects were associated with marked reductions in oxidative stress markers, including serum uric acid, blood urea nitrogen, hepatic xanthine oxidase, and malondialdehyde (MDA) levels in serum, liver, and joint samples, as well as the downregulation of inflammation and uric acid transport-related gene expression in kidney samples. These benefits were comparable to those treated with Febuxostat, a standard urate-lowering therapy for gout. Furthermore, gut microbiome analysis revealed that PMC72 restored dysbiosis induced by hyperuricemia, contrasting with the reduced microbial diversity observed with febuxostat alone, and showed a complete recovery to eubiosis when combined with Febuxostat. These findings position PMC72 as a promising microbial therapeutic candidate for gout management, demonstrating significant development potential and serving as a benchmark for reverse translational microbiome-based therapeutic research.
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Citations
Citations to this article as recorded by  - Characterization of Gut Microbiota of Honey Bees in Korea
Md Sarower Hossen Shuvo, Sukyung Kim, Sujin Jo, Md Abdur Rahim, Indrajeet Barman, Mohammed Solayman Hossain, Yoonkyoung Jeong, Hwasik Jeong, Sangrim Kim, Hoonhee Seo, Ho-Yeon Song
Polish Journal of Microbiology.2025; 74(4): 428. CrossRef - Quantitative assessment of microbial dynamics in livestock manure and municipal wastewater treatment plants
Geon Choi, Hokyung Song, Tatsuya Unno
Applied Biological Chemistry.2025;[Epub] CrossRef - Flavonifractor plautii as a Next-Generation Probiotic Enhancing the NGP F/P Index in a Simulated Human Gut Microbiome Ecosystem
Md Sarower Hossen Shuvo, Sukyung Kim, Sujin Jo, Md Abdur Rahim, Indrajeet Barman, Mohammed Solayman Hossain, Youjin Yoon, Hanieh Tajdozian, Izaz Ahmed, Ali Atashi, GangWon Jeong, Ho-Seong Suh, JiMin You, Chaemin Sung, Mijung Kim, Hoonhee Seo, Ho-Yeon Song
Pharmaceutics.2025; 17(12): 1603. CrossRef
- Characteristics of skin microbiome associated with disease severity in systemic sclerosis
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Kyung-Ann Lee, Asad Ul-Haq, Hoonhee Seo, Sujin Jo, Sukyung Kim, Ho-Yeon Song, Hyun-Sook Kim
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J. Microbiol. 2025;63(1):e.2409018. Published online January 24, 2025
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DOI: https://doi.org/10.71150/jm.2409018
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2,914
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Abstract
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Systemic sclerosis (SSc) is a chronic autoimmune disorder characterised by skin fibrosis and internal organ involvement. Disruptions in the microbial communities on the skin may contribute to the onset of autoimmune diseases that affect the skin. However, current research on the skin microbiome in SSc is lacking. This study aimed to investigate skin microbiome associated with disease severity in SSc. Skin swabs were collected from the upper limbs of 46 healthy controls (HCs) and 36 patients with SSc. Metagenomic analysis based on the 16S rRNA gene was conducted and stratified by cutaneous subtype and modified Rodnan skin score (mRSS) severity. Significant differences in skin bacterial communities were observed between the HCs and patients with SSc, with further significant variations based on subtype and mRSS severity. The identified biomarkers were Bacteroides and Faecalibacterium for patients with diffuse cutaneous SSc with high mRSS (≥ 10) and Mycobacterium and Parabacteroides for those with low mRSS (< 10). Gardnerella, Abies, Lactobacillus, and Roseburia were the biomarkers in patients with limited cutaneous SSc (lcSS) and high mRSS, whereas Coprococcus predominated in patients with lcSS and low mRSS. Cutaneous subtype analysis identified Pediococcus as a biomarker in the HCs, whereas mRSS analysis revealed the presence of Pseudomonas in conjunction with Pediococcus. In conclusion, patients with SSc exhibit distinct skin microbiota compared with healthy controls. Bacterial composition varies by systemic sclerosis cutaneous subtype and skin thickness.
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Citations
Citations to this article as recorded by - Microbiome therapeutic PMC72 through reverse translational research in gout
Mohammed Solayman Hossain, Hoonhee Seo, Kyung-Ann Lee, Asad ul-Haq, Sukyung Kim, Sujin Jo, Md Abdur Rahim, Hanieh Tajdozian, Fatemeh Ghorbanian, Youjin Yoon, Indrajeet Barman, Md Sarower Hossen Shuvo, Hyun-Sook Kim, Ho-Yeon Song
Journal of Microbiology.2025; 63(5): e2501002. CrossRef - Alterations of the skin microbiome in multiple system atrophy: a pilot study
Daji Chen, Lang Sun, Linlin Wan, Zhao Chen, LinLiu Peng, Jinzi Peng, Riwei Ouyang, Xiafei Long, Kefang Du, Xiao Dong, Xiaokang Wu, Xinying Xiao, Ruqing He, Rong Qiu, Beisha Tang, Hong Jiang
npj Parkinson's Disease.2025;[Epub] CrossRef - Analysis of skin mycobiota associated with alopecia in captive cynomolgus macaques (Macaca fascicularis) based on Oxford Nanopore Technologies
Natthanit Phokkhasub, Suthida Visedthorn, Pavit Klomkliew, Prangwalai Chanchaem, Kittima Phutthawong, Taratorn Kemthong, Vorthon Sawaswong, Ariya Khamwut, Suchinda Malaivijitnond, Sunchai Payungporn
F1000Research.2025; 14: 1228. CrossRef - Alterations in the Gut Microbiome in Ankylosing Spondylitis and Their Correlation with Disease Activity
Hyemin Jeong, Hoonhee Seo, Sukyung Kim, Md Abdur Rahim, Indrajeet Barman, Md Sarower Hossen Shuvo, Sujin Jo, Mohammed Solayman Hossain, Jeong-Ju Yoo, Young Ho Kim, Sung-Soo Jung, Ho-Yeon Song, Chan Hong Jeon
Journal of Microbiology and Biotechnology.2025;[Epub] CrossRef
- Vaccine Development for Severe Fever with Thrombocytopenia Syndrome Virus in Dogs
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Seok-Chan Park, Da-Eun Jeong, Sun-Woo Han, Joon-Seok Chae, Joo-Yong Lee, Hyun-Sook Kim, Bumseok Kim, Jun-Gu Kang
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J. Microbiol. 2024;62(4):327-335. Published online April 18, 2024
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DOI: https://doi.org/10.1007/s12275-024-00119-y
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689
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Severe fever with thrombocytopenia syndrome (SFTS) is a life-threatening viral zoonosis. The causative agent of this disease is the Dabie bandavirus, which is usually known as the SFTS virus (SFTSV). Although the role of vertebrates in SFTSV transmission to humans remains uncertain, some reports have suggested that dogs could potentially transmit SFTSV to humans. Consequently, preventive measures against SFTSV in dogs are urgently needed. In the present study, dogs were immunized three times at two-week intervals with formaldehyde-inactivated SFTSV with two types of adjuvants. SFTSV (KCD46) was injected into all dogs two weeks after the final immunization. Control dogs showed viremia from 2 to 4 days post infection (dpi), and displayed white pulp atrophy in the spleen, along with a high level of terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling assay (TUNEL) positive area. However, the inactivated SFTSV vaccine groups exhibited rare pathological changes and significantly reduced TUNEL positive areas in the spleen. Furthermore, SFTSV viral loads were not detected at any of the tested dpi. Our results indicate that both adjuvants can be safely used in combination with an inactivated SFTSV formulation to induce strong neutralizing antibodies. Inactivated SFTSV vaccines effectively prevent pathogenicity and viremia in dogs infected with SFTSV. In conclusion, our study highlighted the potential of inactivated SFTSV vaccination for SFTSV control in dogs.
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Citations
Citations to this article as recorded by - Clinical characteristics of two companion canines with severe fever with thrombocytopenia syndrome virus (Bandavirus dabieense) in Seoul, Republic of Korea
Hye-Ryung Byun, Seong-Ryeong Ji, Jeong-Yeon Hwang, So-Yoon Lee, Joon-Seok Chae
Veterinary Research Communications.2026;[Epub] CrossRef - The immunogenicity and protection efficacy evaluation of mRNA vaccine candidate for severe fever with thrombocytopenia syndrome in mice
Da-Eun Jeong, Jack Yoon, Baek Kim, Jun-Gu Kang, Abdallah M. Samy
PLOS Neglected Tropical Diseases.2025; 19(4): e0012999. CrossRef - Efficient and modular reverse genetics system for rapid generation of recombinant severe acute respiratory syndrome coronavirus 2
Sojung Bae, Jinjong Myoung
Journal of Microbiology.2025; 63(7): e2504015. CrossRef - Current status of severe fever with thrombocytopenia syndrome in China (Review)
Hao Sun, Quanman Hu, Saiwei Lu, Yanyan Yang, Li Zhang, Jinzhao Long, Yuefei Jin, Haiyan Yang, Shuaiyin Chen, Guangcai Duan
International Journal of Molecular Medicine.2025; 56(5): 1. CrossRef - Domain-Specific Impacts of Spike Protein Mutations on Infectivity and Antibody Escape in SARS-CoV-2 Omicron BA.1
Tae-Hun Kim, Sojung Bae, Jinjong Myoung
Journal of Microbiology and Biotechnology.2025;[Epub] CrossRef
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