- Manganese Transporter Proteins in Salmonella enterica serovar Typhimurium
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Nakyeong Ha , Eun-Jin Lee
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J. Microbiol. 2023;61(3):289-296. Published online March 2, 2023
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DOI: https://doi.org/10.1007/s12275-023-00027-7
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The metal cofactors are essential for the function of many enzymes. The host restricts the metal acquisition of pathogens for
their immunity and the pathogens have evolved many ways to obtain metal ions for their survival and growth. Salmonella
enterica serovar Typhimurium also needs several metal cofactors for its survival, and manganese has been found to contribute
to Salmonella pathogenesis. Manganese helps Salmonella withstand oxidative and nitrosative stresses. In addition,
manganese affects glycolysis and the reductive TCA, which leads to the inhibition of energetic and biosynthetic metabolism.
Therefore, manganese homeostasis is crucial for full virulence of Salmonella. Here, we summarize the current information
about three importers and two exporters of manganese that have been identified in Salmonella. MntH, SitABCD, and ZupT
have been shown to participate in manganese uptake. mntH and sitABCD are upregulated by low manganese concentration,
oxidative stress, and host NRAMP1 level. mntH also contains a Mn2+-
dependent riboswitch in its 5′ UTR. Regulation of
zupT expression requires further investigation. MntP and YiiP have been identified as manganese efflux proteins. mntP is
transcr!ptionally activated by MntR at high manganese levels and repressed its activity by MntS at low manganese levels.
Regulation of yiiP requires further analysis, but it has been shown that yiiP expression is not dependent on MntS. Besides
these five transporters, there might be additional transporters that need to be identified.
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Citations
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Antje Müller, Jasmin Schmidt, Verena Maiberg, Oscar Gehring, Adam Schikora
Frontiers in Horticulture.2025;[Epub] CrossRef - Dietary Manganese Modulates Microbiota and Intestinal N‐Acylethanolamines in a Sex‐Specific Manner in Mice With Diet‐Induced Obesity
Fredy Alexander Guevara Agudelo, Nadine Leblanc, Isabelle Bourdeau‐Julien, Gabrielle St‐Arnaud, Fadil Dahhani, Nicolas Flamand, Alain Veilleux, Vincenzo Di Marzo, Frédéric Raymond
The FASEB Journal.2025;[Epub] CrossRef -
Functional characterization of a TerC family protein of
Riemerella anatipestifer
in manganese detoxification and virulence
Qinyuan Chen, Fang Guo, Li Huang, Mengying Wang, Chunfeng Shi, Shutong Zhang, Yizhou Yao, Mingshu Wang, Dekang Zhu, Renyong Jia, Shun Chen, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Bin Tian, Juan Huang, Xumin Ou, Qun Gao, Di Sun, Ling Zhang, Yanling
Applied and Environmental Microbiology.2024;[Epub] CrossRef - NO enhances the adaptability to high-salt environments by regulating osmotic balance, antioxidant defense, and ion homeostasis in eelgrass based on transcriptome and metabolome analysis
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Benjamin X. Wang, Dmitry Leshchiner, Lijuan Luo, Miles Tuncel, Karsten Hokamp, Jay C. D. Hinton, Denise M. Monack
Nature Genetics.2024; 56(6): 1288. CrossRef -
Biological characteristics of manganese transporter MntP in
Klebsiella pneumoniae
Wei Peng, Yafei Xu, Yilin Yin, Jichen Xie, Renhui Ma, Guoyuan Song, Zhiqiang Zhang, Qiuhang Quan, Qinggen Jiang, Moran Li, Bei Li, Michael David Leslie Johnson
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Ludovico Screpanti, Nathalie Desmasures, Margot Schlusselhuber
Critical Reviews in Food Science and Nutrition.2024; : 1. CrossRef - Substrate-Induced Structural Dynamics and Evolutionary Linkage of Siderophore-Iron ABC Transporters of Mycobacterium tuberculosis
Aisha Farhana, Abdullah Alsrhani, Hasan Ejaz, Muharib Alruwaili, Ayman A. M. Alameen, Emad Manni, Zafar Rasheed, Yusuf Saleem Khan
Medicina.2024; 60(11): 1891. CrossRef - Structures and coordination chemistry of transporters involved in manganese and iron homeostasis
Shamayeeta Ray, Rachelle Gaudet
Biochemical Society Transactions.2023; 51(3): 897. CrossRef - Bacterial Regulatory Mechanisms for the Control of Cellular Processes: Simple Organisms’ Complex Regulation
Jin-Won Lee
Journal of Microbiology.2023; 61(3): 273. CrossRef
- Pat- and Pta-mediated protein acetylation is required for horizontallyacquired virulence gene expression in Salmonella Typhimurium
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Hyojeong Koo , Eunna Choi , Shinae Park , Eun-Jin Lee , Jung-Shin Lee
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J. Microbiol. 2022;60(8):823-831. Published online May 27, 2022
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DOI: https://doi.org/10.1007/s12275-022-2095-y
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Salmonella Typhimurium is a Gram-negative facultative pathogen
that causes a range of diseases, from mild gastroenteritis
to severe systemic infection in a variety of animal
hosts. S. Typhimurium regulates virulence gene expression
by a silencing mechanism using nucleoid-associated proteins
such as Histone-like Nucleoid Structuring protein (H-NS)
silencing. We hypothesize that the posttranslational modification,
specifically protein acetylation, of proteins in gene
silencing systems could affect the pathogenic gene expression
of S. Typhimurium. Therefore, we created acetylation-deficient
mutant by deleting two genes, pat and pta, which are
involved in the protein acetylation pathway. We observed
that the pat and pta deletion attenuates mouse virulence and
also decreases Salmonella’s replication within macrophages.
In addition, the Δpat Δpta strain showed a decreased expression
of the horizontally-acquired virulence genes, mgtC,
pagC, and ugtL, which are highly expressed in low Mg2+. The
decreased virulence gene expression is possibly due to higher
H-NS occupancy to those promoters because the pat and
pta deletion increases H-NS occupancy whereas the same
mutation decreases occupancy of RNA polymerase. Our results
suggest that Pat- and Pta-mediated protein acetylation
system promotes the expression of virulence genes by regulating
the binding affinity of H-NS in S. Typhimurium.
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Citations
Citations to this article as recorded by - Reversible acetylation of ribosomal protein S1 serves as a smart switch for Salmonella to rapidly adapt to host stress
Yi-Lin Shen, Tian-Xian Liu, Lei Xu, Bang-Ce Ye, Ying Zhou
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Yu Li, Jinyu Zhang, Ke Wei, Di Zhou, Zepeng Wang, Zhiwei Zeng, Yu Han, Weisheng Cao
ACS Nano.2024; 18(45): 31435. CrossRef
- [Protocol] Detecting Salmonella Type II flagella production by transmission electron microscopy and immunocytochemistry
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Yoontak Han , Eun-Jin Lee
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J. Microbiol. 2020;58(4):245-251. Published online November 23, 2019
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DOI: https://doi.org/10.1007/s12275-020-9297-y
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329
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8
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The bacterial flagellum is an appendage structure that provides
a means for motility to promote survival in fluctuating
environments. For the intracellular pathogen Salmonella enterica
serovar Typhimurium to survive within macrophages,
flagellar gene expression must be tightly regulated, and thus,
is controlled at multiple levels, including DNA recombination,
transcription, post-transcription, protein synthesis, and
assembly within host cells. To understand the contribution of
flagella to Salmonella pathogenesis within the host, it is critical
to detect flagella production within macrophages via
microscopy. In this paper, we describe two methods for detecting
bacterial flagella by microscopy both in vitro and in
vivo infection models.
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Ecotoxicology and Environmental Safety.2022; 240: 113698. CrossRef - Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
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Journal of Microbiology.2021; 59(12): 1133. CrossRef
- A rule governing the FtsH-mediated proteolysis of the MgtC virulence protein from Salmonella enterica serovar Typhimurium
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Jonghyun Baek , Eunna Choi , Eun-Jin Lee
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J. Microbiol. 2018;56(8):565-570. Published online July 25, 2018
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DOI: https://doi.org/10.1007/s12275-018-8245-6
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358
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A tightly controlled turnover of membrane proteins is required
for lipid bilayer stability, cell metabolism, and cell viability.
Among the energy-dependent AAA+ proteases in Salmonella,
FtsH is the only membrane-bound protease that contributes
to the quality control of membrane proteins. FtsH preferentially
degrades the C-terminus or N-terminus of misfolded,
misassembled, or damaged proteins to maintain physiological
functions. We found that FtsH hydrolyzes the Salmonella
MgtC virulence protein when we substitute the MgtC 226th
Trp, which is well conserved in other intracellular pathogens
and normally protects MgtC from the FtsH-mediated proteolysis.
Here we investigate a rule determining the FtsHmediated
proteolysis of the MgtC protein at Trp226 residue.
Substitution of MgtC tryptophan 226th residue to alanine, glycine,
or tyrosine leads to MgtC proteolysis in a manner dependent
on the FtsH protease whereas substitution to phenylalanine,
methionine, isoleucine, leucine, or valine resists
MgtC degradation by FtsH. These data indicate that a large
and hydrophobic side chain at 226th residue is required for
protection from the FtsH-mediated MgtC proteolysis.
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Journal of Microbiology.2019; 57(10): 910. CrossRef - The coordinated action of RNase III and RNase G controls enolase expression in response to oxygen availability in Escherichia coli
Minho Lee, Minju Joo, Minji Sim, Se-Hoon Sim, Hyun-Lee Kim, Jaejin Lee, Minkyung Ryu, Ji-Hyun Yeom, Yoonsoo Hahn, Nam-Chul Ha, Jang-Cheon Cho, Kangseok Lee
Scientific Reports.2019;[Epub] CrossRef
- MINIREVIEW] Regulation and function of the Salmonella MgtC virulence protein
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Jang-Woo Lee , Eun-Jin Lee
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J. Microbiol. 2015;53(10):667-672. Published online August 1, 2015
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DOI: https://doi.org/10.1007/s12275-015-5283-1
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340
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Abstract
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Salmonella enterica serovar Typhimurium produces many
virulence proteins to cause diseases. The Salmonella MgtC
protein is one of such virulence proteins specially required
for intracellular proliferation inside macrophages and mouse
virulence. In this review, we will cover how the mgtC gene
is turned on or off and what the signals required for mgtC
expression are. Later in this review, we will discuss a recent
understanding of MgtC function in Salmonella pathogenesis
by identifying its target proteins.
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Rance Derrick N. Pavon, Windell L. Rivera
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Animals.2020; 10(12): 2418. CrossRef - The mgtCBR mRNA Leader Secures Growth of Salmonella in Both Host and Non-host Environments
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Frontiers in Microbiology.2019;[Epub] CrossRef - Synthetic hydrophobic peptides derived from MgtR weaken Salmonella pathogenicity and work with a different mode of action than endogenously produced peptides
Mariana Rosas Olvera, Preeti Garai, Grégoire Mongin, Eric Vivès, Laila Gannoun-Zaki, Anne-Béatrice Blanc-Potard
Scientific Reports.2019;[Epub] CrossRef - The malS-5′UTR weakens the ability of Salmonella enterica serovar Typhi to survive in macrophages by increasing intracellular ATP levels
Fang Dong, Lin Xia, Renfei Lu, Xin Zhao, Yiquan Zhang, Ying Zhang, Xinxiang Huang
Microbial Pathogenesis.2018; 115: 321. CrossRef - High‐level, constitutive expression of the mgtC gene confers increased thermotolerance on Salmonella enterica serovar Typhimurium
Aaron R. Gall, Annie E. Hegarty, Kirill A. Datsenko, Richard P. Westerman, Phillip SanMiguel, Laszlo N. Csonka
Molecular Microbiology.2018; 109(3): 327. CrossRef - Regulatory RNAs in Virulence and Host-Microbe Interactions
Alexander J. Westermann, Gisela Storz, Kai Papenfort
Microbiology Spectrum.2018;[Epub] CrossRef - Mechanisms of Adaptive Immunity to Porcine Reproductive and Respiratory Syndrome Virus
Michael Rahe, Michael Murtaugh
Viruses.2017; 9(6): 148. CrossRef - Regulating Bacterial Virulence with RNA
Juan J. Quereda, Pascale Cossart
Annual Review of Microbiology.2017; 71(1): 263. CrossRef -
A
trans
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Eunna Choi, Soomin Choi, Daesil Nam, Shinae Park, Yoontak Han, Jung-Shin Lee, Eun-Jin Lee
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- Identification of [sigma]^B-Dependent Promoters Using Consensus-Directed Search of Streptomyces coelicolor Genome
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Eun-Jin Lee , You-Hee Cho , Hyo-Sub Kim , Jung-Hye Roe
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J. Microbiol. 2004;42(2):147-151.
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DOI: https://doi.org/2030 [pii]
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Abstract
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[sigma]^B plays an important role in both osmoprotection and proper differentiation in Streptomyces coelicolor A3(2). We searched for candidate members of the [sigma]^B regulon from the genome database, using the consensus promoter sequence (GNNTN_14-16GGGTAC/T). The list consists of 115 genes, and includes all the known [sigma]^B target genes and many other genes whose functions are related to stress protection and differentiation.
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