- Lytic KFS-SE2 phage as a novel bio-receptor for Salmonella Enteritidis detection
-
In Young Choi , Cheonghoon Lee , Won Keun Song , Sung Jae Jang , Mi-Kyung Park
-
J. Microbiol. 2019;57(2):170-179. Published online January 31, 2019
-
DOI: https://doi.org/10.1007/s12275-019-8610-0
-
-
435
View
-
0
Download
-
14
Web of Science
-
14
Crossref
-
 Abstract
 PDF
-
Since Salmonella Enteritidis is one of the major foodborne
pathogens, on-site applicable rapid detection methods have
been required for its control. The purpose of this study was
to isolate and purify S. Enteritidis-specific phage (KFS-SE2
phage) from an eel farm and to investigate its feasibility as a
novel, efficient, and reliable bio-receptor for its employment.
KFS-SE2 phage was successfully isolated at a high concentration
of (2.31 ± 0.43) × 1011 PFU/ml, and consisted of an
icosahedral head of 65.44 ± 10.08 nm with a non-contractile
tail of 135.21 ± 12.41 nm. The morphological and phylogenetic
analysis confirmed that it belongs to the Pis4avirus genus
in the family of Siphoviridae. KFS-SE2 genome consisted
of 48,608 bp with 45.7% of GC content. Genome analysis
represented KFS-SE2 to have distinctive characteristics as a
novel phage. Comparative analysis of KFS-SE2 phage with
closely related strains confirmed its novelty by the presence
of unique proteins. KFS-SE2 phage exhibited excellent specificity
to S. Enteritidis and was stable under the temperature
range of 4 to 50°C and pH of 3 to 11 (P < 0.05). The latent time
was determined to be 20 min. Overall, a new lytic KFS-SE2
phage was successfully isolated from the environment at a
high concentration and the excellent feasibility of KFS-SE2
phage was demonstrated as a new bio-receptor for S. Enteritidis
detection.
-
Citations
Citations to this article as recorded by  - Phage vB_KlebPS_265 Active Against Resistant/MDR and Hypermucoid K2 Strains of Klebsiella pneumoniae
Vyacheslav I. Yakubovskij, Vera V. Morozova, Yuliya N. Kozlova, Artem Yu. Tikunov, Valeria A. Fedorets, Elena V. Zhirakovskaya, Igor V. Babkin, Alevtina V. Bardasheva, Nina V. Tikunova
Viruses.2025; 17(1): 83. CrossRef - Functional and Genomic Features of a Lytic
Salmonella Phage vB_StyS_KFSST1 for
Development as New Feed Additive
Su-Hyeon Kim, In Young Choi, Gyu-Sung Cho, Charles M.A.P. Franz, Mi-Kyung Park
Food Science of Animal Resources.2025; 45(4): 1204. CrossRef - Lytic Spectra of Tailed Bacteriophages: A Systematic Review and Meta-Analysis
Ivan M. Pchelin, Andrei V. Smolensky, Daniil V. Azarov, Artemiy E. Goncharov
Viruses.2024; 16(12): 1879. CrossRef - User-friendly, signal-enhanced planar spiral coil-based magnetoelastic biosensor combined with humidity-resistant phages for simultaneous detection of Salmonella Typhimurium and Escherichia coli O157:H7 on fresh produce
In Young Choi, Jaein Choe, Bryan A. Chin, Mi-Kyung Park
Sensors and Actuators B: Chemical.2023; 393: 134179. CrossRef - Performance of wild, tailed, humidity-robust phage on a surface-scanning magnetoelastic biosensor for Salmonella Typhimurium detection
Hwa-Eun Lee, Yu-Bin Jeon, Bryan A. Chin, Sang Hyuk Lee, Hye Jin Lee, Mi-Kyung Park
Food Chemistry.2023; 409: 135239. CrossRef - Advances in detection methods for viable Salmonella spp.: current applications and challenges
Linlin Zhuang, Jiansen Gong, Qiuping Shen, Jianbo Yang, Chunlei Song, Qingxin Liu, Bin Zhao, Yu Zhang, Mengling Zhu
Analytical Sciences.2023; 39(10): 1643. CrossRef - Prevalence of Indigenous Antibiotic-Resistant Salmonella Isolates and Their Application to Explore a Lytic Phage vB_SalS_KFSSM with an Intra-Broad Specificity
Jaein Choe, Su-Hyeon Kim, Ji Min Han, Jong-Hoon Kim, Mi-Sun Kwak, Do-Won Jeong, Mi-Kyung Park
Journal of Microbiology.2023; 61(12): 1063. CrossRef - Breathing‐Driven Self‐Powered Pyroelectric ZnO Integrated Face Mask for Bioprotection
Moon‐Ju Kim, Zhiquan Song, Chang Kyu Lee, Tae Gyeong Yun, Joo‐Yoon Noh, Mi‐Kyung Park, Dongeun Yong, Min‐Jung Kang, Jae‐Chul Pyun
Small.2023;[Epub] CrossRef - Phage-targeting bimetallic nanoplasmonic biochip functionalized with bacterial outer membranes as a biorecognition element
Moon-Ju Kim, Hyung Eun Bae, Soonil Kwon, Mi-Kyung Park, Dongeun Yong, Min-Jung Kang, Jae-Chul Pyun
Biosensors and Bioelectronics.2023; 238: 115598. CrossRef - Salmonella phage akira, infecting selected Salmonella enterica Enteritidis and Typhimurium strains, represents a new lineage of bacteriophages
Nikoline S. Olsen, René Lametsch, Natalia Wagner, Lars Hestbjerg Hansen, Witold Kot
Archives of Virology.2022; 167(10): 2049. CrossRef - Bacteriophage-Based Biosensors: A Platform for Detection of Foodborne Bacterial Pathogens from Food and Environment
Rashad R. Al-Hindi, Addisu D. Teklemariam, Mona G. Alharbi, Ibrahim Alotibi, Sheren A. Azhari, Ishtiaq Qadri, Turki Alamri, Steve Harakeh, Bruce M. Applegate, Arun K. Bhunia
Biosensors.2022; 12(10): 905. CrossRef - Characterization of a New and Efficient Polyvalent Phage Infecting E. coli O157:H7, Salmonella spp., and Shigella sonnei
Su-Hyeon Kim, Damilare Emmanuel Adeyemi, Mi-Kyung Park
Microorganisms.2021; 9(10): 2105. CrossRef - Improvement of a new selective enrichment broth for culturing Salmonella in ready‐to‐eat fruits and vegetables
Jiajia Wan, Zhaoxin Lu, Xiaomei Bie, Fengxia Lv, Haizhen Zhao
Journal of Food Safety.2020;[Epub] CrossRef - Exploring the feasibility of Salmonella
Typhimurium-specific phage as a novel bio-receptor
In Young Choi, Do Hyeon Park, Brayan A. Chin, Cheonghoon Lee, Jinyoung Lee, Mi-Kyung Park
Journal of Animal Science and Technology.2020; 62(5): 668. CrossRef
- Evaluation of the Sensitivity and Specificity of Primer Pairs and the Efficiency of RNA Extraction Procedures to Improve Noroviral Detection from Oysters by Nested Reverse Transcription-Polymerase Chain Reaction
-
Cheonghoon Lee , Sooryun Cheong , Hee-Jung Lee , Miye Kwon , Ilnam Kang , Eun-Gyoung Oh , Hong-Sik Yu , Soon-Bum Shin , Sang-Jong Kim
-
J. Microbiol. 2010;48(5):586-593. Published online November 3, 2010
-
DOI: https://doi.org/10.1007/s12275-010-0047-4
-
-
226
View
-
0
Download
-
6
Scopus
-
Abstract
PDF
-
Noroviruses (NoV) are the key cause of acute epidemic gastroenteritis, and oysters harvested from NoVpolluted sea areas are considered as the significant vectors of viral transmission. To improve NoV detection from oyster using nested reverse transcription-polymerase chain reaction (RT-PCR), we evaluated the sensitivity and specificity of previously published primer pairs and the efficiency of different RNA extraction procedures. Among the primer pairs used for RT-PCR, the sensitivity of GIF1/GIR1-GIF2/GIR1 and GIIF1/GIIR1-GIIF2/GIIR1 was higher than that of other primer pairs used in nested RT-PCR for the detection of NoV genogroup I (NoV GI) and NoV GII from both NoV-positive stool suspension and NoVseeded oyster concentrates, respectively; the resulting products showed neither unspecific bands in the positive samples nor false-positive bands in the negative controls. The extraction of NoV RNA from oyster samples using a QIAamp? Viral RNA Mini kit with a QIAshredderTM Homogenizer pretreatment afforded more efficient recovery (mean recovery for NoV GI and GII, 6.4%) and the procedure was less time consuming (<30 min) than most other RNA extraction procedures. The results of RNA extraction procedure and primer pairs evaluated by nested RT-PCR assay in this study can be useful for monitoring NoV contamination in oysters, which is an indicator of possible public health risks.
|
MSK
