- The inability of Bacillus licheniformis perR mutant to grow is mainly due to the lack of PerR-mediated fur repression
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Jung-Hoon Kim , Yoon-Mo Yang , Chang-Jun Ji , Su-Hyun Ryu , Young-Bin Won , Shin-Yeong Ju , Yumi Kwon , Yeh-Eun Lee , Hwan Youn , Jin-Won Lee
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J. Microbiol. 2017;55(6):457-463. Published online April 22, 2017
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DOI: https://doi.org/10.1007/s12275-017-7051-x
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PerR, a member of Fur family protein, is a metal-dependent H2O2 sensing transcription factor that regulates genes in-volved in peroxide stress response. Industrially important bac-terium Bacillus licheniformis contains three PerR-like pro-teins (PerRBL, PerR2, and PerR3) compared to its close rela-tive Bacillus subtilis. Interestingly, unlike other bacteria in-cluding B. subtilis, no authentic perRBL null mutant could be established for B. licheniformis. Thus, we constructed a con-ditional perRBL mutant using a xylose-inducible promoter, and investigated the genes under the control of PerRBL. PerRBL regulon genes include katA, mrgA, ahpC, pfeT, hemA, fur, and perR as observed for PerRBS. However, there is some variation in the expression levels of fur and hemA genes be-tween B. subtilis and B. licheniformis in the derepressed state. Furthermore, katA, mrgA, and ahpC are strongly induced, whereas the others are only weakly or not induced by H2O2 treatment. In contrast to the B. subtilis perR null mutant which frequently gives rise to large colony phenotype mainly due to the loss of katA, the suppressors of B. licheniformis perR mutant, which can form colonies on LB agar, were all cata-lase-positive. Instead, many of the suppressors showed in-creased levels of siderophore production, suggesting that the suppressor mutation is linked to the fur gene. Consistent with this, perR fur double mutant could grow on LB agar without Fe supplementation, whereas perR katA double mutant could only grow on LB agar with Fe supplementation. Taken toge-ther, our data suggest that in B. licheniformis, despite the si-milarity in PerRBL and PerRBS regulon genes, perR is an essen-tial gene required for growth and that the inability of perR null mutant to grow is mainly due to elevated expression of Fur.
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Citations
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Lu Liu, Dehua Luo, Yongji Zhang, Dingqi Liu, Kang Yin, Qing Tang, Shan-Ho Chou, Jin He, Beile Gao
Microbiology Spectrum.2024;[Epub] CrossRef - Meddling with Metal Sensors: Fur-Family Proteins as Signaling Hubs
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Analytical Chemistry.2019; 91(15): 10064. CrossRef - Redox Sensing by Fe2+in Bacterial Fur Family Metalloregulators
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Antioxidants & Redox Signaling.2018; 29(18): 1858. CrossRef
- Author’s Correction] Experimental Phasing Using Zinc and Sulfur Anomalous Signals Measured at the Zinc Absorption Peak
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Sangmin Lee , Min-Kyu Kim , Chang-Jun Ji , Jin-Won Lee , Sun-Shin Cha
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J. Microbiol. 2013;51(6):886-886.
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DOI: https://doi.org/10.1007/s12275-013-0725-5
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In the article by Lee et al. published in the Journal of Microbiology 2013; 51, 639-643. Acknowledgement should appear as
shown below.
This work was supported by the National Research Foundation of Korea Grant NRF-2012R1A2A2A02005978, the CAP
through Korea Research Council of Fundamental Science Technology (KRCF), Korea Institute of Science and Technology
(KIST), & Korea Institute of Ocean Science and Technology (KIOST), the Marine and Extreme Genome Research Center
program, and the Development of Biohydrogen Production Technology Using Hyperthermophilic Archaea program of MOF.
Works performed at Hanyang University were supported by Basic Science Research Program through the National Research
Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (MEST) (KRF-2008-313-C00774).
- Experimental Phasing Using Zinc and Sulfur Anomalous Signals Measured at the Zinc Absorption Peak
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Sangmin Lee , Min-Kyu Kim , Chang-Jun Ji , Jin-Won Lee , Sun-Shin Cha
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J. Microbiol. 2013;51(5):639-643. Published online October 31, 2013
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DOI: https://doi.org/10.1007/s12275-013-3412-2
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Iron is an essential transition metal required for bacterial growth and survival. Excess free iron can lead to the generation of reactive oxygen species that can cause severe damage to cellular functions. Cells have developed iron-sensing regulators to maintain iron homeostasis at the transcription level. The ferric uptake regulator (Fur) is an iron-responsive regulator that controls the expression of genes involved in iron homeostasis, bacterial virulence, stress resistance, and redox metabolism. Here, we report the expression, purification, crystallization, and phasing of the apo-form of Bacillus subtilis Fur (BsFur) in the absence of regulatory metal ions. Crystals were obtained by microbatch crystallization method at 295 K and diffraction data at a resolution of 2.6 Å was collected at the zinc peak wavelength (λ=1.2823 Å). Experimental phasing identified the positions of one zinc atom and four sulfur atoms of cysteine residues coordinating the zinc atom, indicating that the data contained a meaningful anomalous scattering originating from the ordered zinc-coordinating sulfur atoms, in spite of the small anomalous signals of sulfur atoms at the examined wavelength.
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