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- Regulatory role of cysteines in (2R, 3R)-butanediol dehydrogenase BdhA of Bacillus velezensis strain GH1-13
- Yunhee Choi , Yong-Hak Kim
- J. Microbiol. 2022;60(4):411-418. Published online March 14, 2022
- DOI: https://doi.org/10.1007/s12275-022-2018-y
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Abstract
- Bacillus velezensis strain GH1-13 contains a (2R,3R)-butanediol dehydrogenase (R-BDH) BdhA which converts acetoin to R-BD reversibly, however, little is known about its regulatory cysteine and biological significance. We performed sitedirected mutation of three cysteines in BdhA. The C37S mutant had no enzyme activity and the C34S and C177S mutants differed from each other and wild type (WT). After zinc affinity chromatography, 1 mM ZnCl2 treatment resulted in a 3-fold enhancement of the WT activity, but reduced activity of the C34S mutant by more than 2 folds compared to the untreated ones. However, ZnCl2 treatment did not affect the activity of the C177S mutant. Most of the double and triple mutant proteins (C34S/C37S, C34S/C177S, C37S/C177S, and C34S/C37S/C177S) were aggregated in zinc resins, likely due to the decreased protein stability. All of the purified WT and single mutant proteins increased multiple intermolecular disulfide bonds in the presence of H2O2 as the buffer pH decreased from 7.5 to 5.5, whereas an intramolecular disulfide bond of cysteine 177 and another cysteine in the CGIC motif region was likely formed at pH higher than pKa of 7.5. When pH varied, WT and its C34S or C177S mutants reduced acetoin to R-BD at the optimum pH 5.5 and oxidized R-BD to acetoin at the optimum pH 10. This study demonstrated that cysteine residues in BdhA play a regulatory role for the production of acetoin and R-BD depending on pH as well as metal binding and oxidative stress.
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- Significantly enhanced specific activity of Bacillus subtilis (2,3)-butanediol dehydrogenase through computer-aided refinement of its substrate-binding pocket
Bochun Hu, Xiaoqi Xi, Fugang Xiao, Xiaomeng Bai, Yuanyuan Gong, Yifan Li, Xueqin Qiao, Cunduo Tang, Jihong Huang
International Journal of Biological Macromolecules.2024; 281: 136443. CrossRef - Structural and enzymatic characterization of Bacillus subtilis R,R-2,3-butanediol dehydrogenase
Xiaofei Wang, Lingyun Jia, Fangling Ji
Biochimica et Biophysica Acta (BBA) - General Subjects.2023; 1867(4): 130326. CrossRef - Engineering a BsBDHA substrate-binding pocket entrance for the improvement in catalytic performance toward (R)-phenyl-1,2-ethanediol based on the computer-aided design
Bo-Chun Hu, Meng-Ran Li, Ying-Ying Li, Xin-Shuang Yuan, Yu-Ye Hu, Fu-Gang Xiao
Biochemical Engineering Journal.2023; 194: 108907. CrossRef
- Significantly enhanced specific activity of Bacillus subtilis (2,3)-butanediol dehydrogenase through computer-aided refinement of its substrate-binding pocket
- Oxygen-mediated growth enhancement of an obligate anaerobic archaeon Thermococcus onnurineus NA1
- Seong Hyuk Lee , Hwan Youn , Sung Gyun Kang , Hyun Sook Lee
- J. Microbiol. 2019;57(2):138-142. Published online January 31, 2019
- DOI: https://doi.org/10.1007/s12275-019-8592-y
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- 3 Web of Science
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Abstract
- Thermococcus onnurineus NA1, an obligate anaerobic hyperthermophilic archaeon, showed variable oxygen (O2) sensitivity depending on the types of substrate employed as an energy source. Unexpectedly, the culture with yeast extract as a sole energy source showed enhanced growth by 2-fold in the presence of O2. Genome-wide transcriptome analysis revealed the upregulation of several antioxidant-related genes encoding thioredoxin peroxidase (TON_0862), rubrerythrin (TON_0864), rubrerythrin-related protein (TON_0873), NAD(P)H rubredoxin oxidoreductase (TON_0865), or thioredoxin reductase (TON_1603), which can couple the detoxification of reactive oxygen species with the regeneration of NAD(P)+ from NAD(P)H. We present a plausible mechanism by which O2 serves to maintain the intracellular redox balance. This study demonstrates an unusual strategy of an obligate anaerobe underlying O2-mediated growth enhancement despite not having heme-based or cytochrome-type proteins.
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Citations
Citations to this article as recorded by- How low can they go? Aerobic respiration by microorganisms under apparent anoxia
Jasmine S Berg, Soeren Ahmerkamp, Petra Pjevac, Bela Hausmann, Jana Milucka, Marcel M M Kuypers
FEMS Microbiology Reviews.2022;[Epub] CrossRef - Reductive evolution and unique predatory mode in the CPR bacterium Vampirococcus lugosii
David Moreira, Yvan Zivanovic, Ana I. López-Archilla, Miguel Iniesto, Purificación López-García
Nature Communications.2021;[Epub] CrossRef
- How low can they go? Aerobic respiration by microorganisms under apparent anoxia
- Transcriptome analysis of differential gene expression in Dichomitus squalens during interspecific mycelial interactions and the potential link with laccase induction
- Zixuan Zhong , Nannan Li , Binghui He , Yasuo Igarashi , Feng Luo
- J. Microbiol. 2019;57(2):127-137. Published online September 13, 2018
- DOI: https://doi.org/10.1007/s12275-019-8398-y
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- 9 Web of Science
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Abstract
- Interspecific mycelial interactions between white rot fungi are always accompanied by an increased production of laccase. In this study, the potential of the white rot fungus Dichomitus squalens to enhance laccase production during interactions with two other white rot fungi, Trametes versicolor or Pleurotus ostreatus, was assessed. To probe the mechanism of laccase induction and the role that laccase plays during combative interaction, we analyzed the differential gene expression profile of the laccase induction response to stressful conditions during fungal interaction. We further confirmed the expression patterns of 16 selected genes by qRT-PCR analysis. We noted that many differentially expressed genes (DEGs) encoded proteins that were involved in xenobiotic detoxification and reactive oxygen species (ROS) generation or reduction, including aldo/keto reductase, glutathione S-transferases, cytochrome P450 enzymes, alcohol oxidases and dehydrogenase, manganese peroxidase and laccase. Furthermore, many DEG-encoded proteins were involved in antagonistic mechanisms of nutrient acquisition and antifungal properties, including glycoside hydrolase, glucanase, chitinase and terpenoid synthases. DEG analyses effectively revealed that laccase induction was likely caused by protective responses to oxidative stress and nutrient competition during interspecific fungal interactions.
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Citations
Citations to this article as recorded by- Lignin-degrading enzyme production was enhanced by the novel transcription factor Ptf6 in synergistic microbial co-culture
Qi Zhang, Qiong Wang, Haixiu Chen, Lei Chen, Feng Wang, Zhenghua Gu, Guiyang Shi, Liming Liu, Zhongyang Ding
Microbiological Research.2024; 280: 127575. CrossRef - Agrobacterium tumefaciens-mediated transformation of the white-rot fungus Dichomitus squalens
Jing Li, Min Wu, Yasuo Igarashi, Feng Luo, Peng Chang
Journal of Microbiological Methods.2023; 214: 106842. CrossRef - Lignin degradation by co-cultured fungi: current status and future perspectives
Jullio Kennedy Castro Soares, Vera Maria Valle Vitali, Marcelo Afonso Vallim
Lilloa.2022; : 39. CrossRef - Coprinopsis cinerea Uses Laccase Lcc9 as a Defense Strategy To Eliminate Oxidative Stress during Fungal‐Fungal Interactions
Juanjuan Liu, Can Peng, Qiqi Han, Mengyao Wang, Gang Zhou, Bin Ye, Yazhong Xiao, Zemin Fang, Ursula Kües, Irina S. Druzhinina
Applied and Environmental Microbiology.2022;[Epub] CrossRef - Construction of a fungal consortium for effective degradation of rice straw lignin and potential application in bio-pulping
Jinghong Wang, Lingling Li, Hongmin Xu, Yali Zhang, Yuxin Liu, Fangzheng Zhang, Guinan Shen, Lei Yan, Weiwei Wang, Hongzhi Tang, Huajiao Qiu, Ji-Dong Gu, Weidong Wang
Bioresource Technology.2022; 344: 126168. CrossRef - Melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi
Samim Dullah, Dibya Jyoti Hazarika, Gunajit Goswami, Tanushree Borgohain, Alokesh Ghosh, Madhumita Barooah, Ashok Bhattacharyya, Robin Chandra Boro
IMA Fungus.2021;[Epub] CrossRef - Fungal interactions induce changes in hyphal morphology and enzyme production
Samim Dullah, Dibya Jyoti Hazarika, Assma Parveen, Merilin Kakoti, Tanushree Borgohain, Trishnamoni Gautom, Ashok Bhattacharyya, Madhumita Barooah, Robin Chandra Boro
Mycology.2021; 12(4): 279. CrossRef - Biological activities of a polysaccharide from the coculture of Ganoderma lucidum and Flammulina velutipes mycelia in submerged fermentation
Jinyu Wu, Khwanta Kaewnarin, Xiaomeng Nie, Qingbiao Li, Ning He, Jiale Huang, Anli Geng
Process Biochemistry.2021; 109: 10. CrossRef - Comparative transcriptomics and transcriptional regulation analysis of enhanced laccase production induced by co-culture of Pleurotus eryngii var. ferulae with Rhodotorula mucilaginosa
Qi Zhang, Liting Zhao, YouRan Li, Feng Wang, Song Li, Guiyang Shi, Zhongyang Ding
Applied Microbiology and Biotechnology.2020; 104(1): 241. CrossRef
- Lignin-degrading enzyme production was enhanced by the novel transcription factor Ptf6 in synergistic microbial co-culture
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