Journal of Microbiology

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Roseovarius tibetensis sp. nov., a halophilic bacterium isolated from Lake LongmuCo on Tibetan Plateau: Hui-bin Lu , Xiao-feng Xue , Dorji Phurbu , Peng Xing , Qing-long Wu; J. Microbiol. 2018;56(11):783-789. Published online October 24, 2018; DOI: https://doi.org/10.1007/s12275-018-8178-0

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Two Gram-stain negative halophilic strains, designated as LM2T and LM4, were isolated from Lake LongmuCo on Tibetan Plateau. These two strains were aerobic, catalaseand oxidase- positive, nonmotile and rod-shaped organisms. Phylogenetic analysis based on 16S rRNA gene sequences indicated that LM2T and LM4 belong to the genus Roseovarius, with Roseovarius tolerans EL-172T (97.3% and 97.4% 16S rRNA gene sequence similarity, respectively) and Roseovarius azorensis SSW084T (95.5% and 95.6% 16S rRNA gene sequence similarity, respectively) as their closest neighbors. Q-10 was the sole respiratory quinone of these two strains. The major fatty acids were C18:1 ω7c/C18:1 ω6c, C16:0, C19:0 cyclo ω8c, and 11-methyl C18:1 ω7c. The polar lipids included phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phospholipid of unknown structure containing glucosamine, and unidentified aminolipid. The DNA G + C content was between 64.2 and 64.5 mol%. DNA-DNA hybridization showed 96.7% relatedness between LM2T and LM4, 24.9% relatedness between LM2T and R. tolerans EL-172T, and 36.3% relatedness between LM4 and R. tolerans EL-172T. Based on phylogenetic analysis, DNA-DNA hybridization, a range of physiological and biochemical characteristics, LM2T and LM4 belong to the same species and were clearly distinguished from the type strains of the genus Roseovarius. It was evident that LM2T and LM4 could be classified as a novel species of the genus Roseovarius, for which the name Roseovarius tibetensis sp. nov. is proposed. The type strain is LM2T (= CGMCC 1.16230T = KCTC 62028T).

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Lithoautotrophic lifestyle of the widespread genusRoseovariusrevealed by physiological and genomic characterization ofRoseovarius autotrophicussp. nov
Galina Slobodkina, Nataliya Ratnikova, Alexander Merkel, Vadim Kevbrin, Alexandra Kuchierskaya, Alexander Slobodkin
FEMS Microbiology Ecology.2022;[Epub] CrossRef
Duganella rivus sp. nov., Duganella fentianensis sp. nov., Duganella qianjiadongensis sp. nov. and Massilia guangdongensis sp. nov., isolated from subtropical streams in China and reclassification of all species within genus Pseudoduganella
Hui-Bin Lu, Zhi-Peng Cai, Yong-Gang Yang, Mei-Ying Xu
Antonie van Leeuwenhoek.2020; 113(8): 1155. CrossRef
Massilia aquatica sp. nov., Isolated from a Subtropical Stream in China
Hui-Bin Lu, Tong-Chu Deng, Mei-Ying Xu
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Halomonas rituensis sp. nov. and Halomonas zhuhanensis sp. nov., isolated from natural salt marsh sediment on the Tibetan Plateau
Peixin Gao, Huibin Lu, Peng Xing, Qinglong L. Wu
International Journal of Systematic and Evolutionary Microbiology .2020; 70(10): 5217. CrossRef
Roseovarius spongiae sp. nov., a bacterium isolated from marine sponge
Lingping Zhuang, Lianzhong Luo
International Journal of Systematic and Evolutionary Microbiology .2020; 70(1): 274. CrossRef
List of new names and new combinations previously effectively, but not validly, published
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International Journal of Systematic and Evolutionary Microbiology.2019; 69(3): 597. CrossRef

Halomonas tibetensis sp. nov., isolated from saline lakes on Tibetan Plateau: Hui-bin Lu , Peng Xing , Lei Zhai , Dorji Phurbu , Qian Tang , Qing-long Wu; J. Microbiol. 2018;56(7):493-499. Published online June 14, 2018; DOI: https://doi.org/10.1007/s12275-018-8076-5

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Abstract

Strains pyc13T and ZGT13 were isolated from Lake Pengyan and Lake Zigetang on Tibetan Plateau, respectively. Both strains were Gram-negative, catalase- and oxidase-positive, aerobic, rod-shaped, nonmotile, and nonflagellated bacteria. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains pyc13T and ZGT13 belong to the genus Halomonas, with Halomonas alkalicola 56-L4-10aEnT as their closest neighbor, showing 97.4% 16S rRNA gene sequence similarity. The predominant respiratory quinone of both strains was Q-9, with Q-8 as a minor component. The major fatty acids of both strains were C18:1 ω6c/C18:1 ω7c, C16:1 ω6c/C16:1 ω7c, C16:0, and C12:0 3OH. The polar lipids of both strains consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, glycolipid, phospholipids of unknown structure containing glucosamine, and unidentified phospholipids. The DNA G + C content of pyc13T and ZGT13 were 62.6 and 63.4 mol%, respectively. The DNA-DNA hybridization values of strain pyc13T were 34, 41, 61, 35, and 35% with the reference strains H. alkalicola 56-L4-10aEnT, H. sediminicola CPS11T, H. mongoliensis Z-7009T, H. ventosae Al12T, and H. fontilapidosi 5CRT, respectively. Phenotypic, biochemical, genotypic, and DNA-DNA hybridization data showed that strains pyc13T and ZGT13 represent a new species within the genus Halomonas, for which the name H. tibetensis sp. nov. is proposed. The type strain is pyc13T (= CGMCC 1.15949T = KCTC 52660T).

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The Bacterial Microbiota of Artisanal Cheeses from the Northern Caucasus
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Fermentation.2023; 9(8): 719. CrossRef
Halomonas profundi sp. nov., isolated from deep-sea sediment of the Mariana Trench
Fan Wang, Jin-Jian Wan, Xi-Ying Zhang, Yu Xin, Mei-Ling Sun, Peng Wang, Wei-Peng Zhang, Ji-Wei Tian, Yu-Zhong Zhang, Chun-Yang Li, Hui-Hui Fu
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef
Halomonas jincaotanensis sp. nov., isolated from the Pamir Plateau degrading polycyclic aromatic hydrocarbon
Xuying Bu, Zhanfeng Xia, Zhanwen Liu, Min Ren, Chuanxing Wan, Lili Zhang
Archives of Microbiology.2022;[Epub] CrossRef
Halomonas rituensis sp. nov. and Halomonas zhuhanensis sp. nov., isolated from natural salt marsh sediment on the Tibetan Plateau
Peixin Gao, Huibin Lu, Peng Xing, Qinglong L. Wu
International Journal of Systematic and Evolutionary Microbiology .2020; 70(10): 5217. CrossRef
Halomonas montanilacus sp. nov., isolated from hypersaline Lake Pengyanco on the Tibetan Plateau
Huibin Lu, Peng Xing, Lei Zhai, Huabing Li, Qinglong Wu
International Journal of Systematic and Evolutionary Microbiology .2020; 70(4): 2859. CrossRef
List of new names and new combinations previously effectively, but not validly, published
Aharon Oren, George M. Garrity
International Journal of Systematic and Evolutionary Microbiology.2018; 68(11): 3379. CrossRef

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