Journal of Microbiology

Journal Articles

Pat- and Pta-mediated protein acetylation is required for horizontallyacquired virulence gene expression in Salmonella Typhimurium: Hyojeong Koo , Eunna Choi , Shinae Park , Eun-Jin Lee , Jung-Shin Lee; J. Microbiol. 2022;60(8):823-831. Published online May 27, 2022; DOI: https://doi.org/10.1007/s12275-022-2095-y

59 View
0 Download
2 Web of Science
1 Crossref

Salmonella Typhimurium is a Gram-negative facultative pathogen that causes a range of diseases, from mild gastroenteritis to severe systemic infection in a variety of animal hosts. S. Typhimurium regulates virulence gene expression by a silencing mechanism using nucleoid-associated proteins such as Histone-like Nucleoid Structuring protein (H-NS) silencing. We hypothesize that the posttranslational modification, specifically protein acetylation, of proteins in gene silencing systems could affect the pathogenic gene expression of S. Typhimurium. Therefore, we created acetylation-deficient mutant by deleting two genes, pat and pta, which are involved in the protein acetylation pathway. We observed that the pat and pta deletion attenuates mouse virulence and also decreases Salmonella’s replication within macrophages. In addition, the Δpat Δpta strain showed a decreased expression of the horizontally-acquired virulence genes, mgtC, pagC, and ugtL, which are highly expressed in low Mg2+. The decreased virulence gene expression is possibly due to higher H-NS occupancy to those promoters because the pat and pta deletion increases H-NS occupancy whereas the same mutation decreases occupancy of RNA polymerase. Our results suggest that Pat- and Pta-mediated protein acetylation system promotes the expression of virulence genes by regulating the binding affinity of H-NS in S. Typhimurium.

Citations

Citations to this article as recorded by

Multi-Lasso Peptide-Based Synergistic Nanocomposite: A High-Stability, Broad-Spectrum Antimicrobial Agent with Potential for Combined Antibacterial Therapy
Yu Li, Jinyu Zhang, Ke Wei, Di Zhou, Zepeng Wang, Zhiwei Zeng, Yu Han, Weisheng Cao
ACS Nano.2024; 18(45): 31435. CrossRef

Rhizocompartments and environmental factors affect microbial composition and variation in native plants: Myung-Suk Kang , Moonsuk Hur , Soo-Je Park; J. Microbiol. 2019;57(7):550-561. Published online June 27, 2019; DOI: https://doi.org/10.1007/s12275-019-8646-1

61 View
0 Download
10 Web of Science
9 Crossref

Abstract

Molecular analysis based on large-scale sequencing of the plant microbiota has revealed complex relationships between plants and microbial communities, and environmental factors such as soil type can influence these relationships. However, most studies on root-associated microbial communities have focused on model plants such as Arabidopsis, rice or crops. Herein, we examined the microbiota of rhizocompartments of two native plants, Sedum takesimense Nakai and Campanula takesimana Nakai, using archaeal and bacterial 16S rRNA gene amplicon profiling, and assessed relationships between environmental factors and microbial community composition. We identified 390 bacterial genera, including known plant-associated genera such as Pseudomonas, Flavobacterium, Bradyrhizobium and Rhizobium, and uncharacterized clades such as DA101 that might be important in root-associated microbial communities in bulk soil. Unexpectedly, Nitrososphaera clade members were abundant, indicating functional association with roots. Soil texture/type has a greater impact on microbial community composition in rhizocompartments than chemical factors. Our results provide fundamental knowledge on microbial diversity, community and correlations with environmental factors, and expand our understanding of the microbiota in rhizocompartments of native plants.

Citations

Citations to this article as recorded by

Ammonium bicarbonate alleviates apple replant disease: Inhibiting Fusarium and improving soil environment
Mei Wang, Li Xiang, Weixiao Tang, Xuesen Chen, Chuanrong Li, Chengmiao Yin, Zhiquan Mao
Scientia Horticulturae.2024; 325: 112652. CrossRef
Regulation of root-associated microbiomes and root exudates by different tobacco species
Mengli Gu, Jingjing Jin, Peng Lu, Shizhou Yu, Huan Su, Haihong Shang, Zhixiao Yang, Jianfeng Zhang, Peijian Cao, Jiemeng Tao
Chemical and Biological Technologies in Agriculture.2024;[Epub] CrossRef
MAPK Cascades in Plant Microbiota Structure and Functioning
Thijs Van Gerrewey, Hoo Sun Chung
Journal of Microbiology.2024; 62(3): 231. CrossRef
Land-use change alters the bacterial community structure, but not forest management
Viviana Rodríguez Rivera, Yendi E. Navarro-Noya, Luc Dendooven, Marco Luna Guido
Folia Microbiologica.2023; 68(2): 277. CrossRef
Soil Microbial Communities Associated with Three Arctic Plants in Different Local Environments in Ny–Ålesund, Svalbard
Deokjoo Son, Eun Ju Lee
Journal of Microbiology and Biotechnology.2022; 32(10): 1275. CrossRef
The root microbiome: Community assembly and its contributions to plant fitness
Bo Bai, Weidong Liu, Xingyu Qiu, Jie Zhang, Jingying Zhang, Yang Bai
Journal of Integrative Plant Biology.2022; 64(2): 230. CrossRef
Full Issue PDF

Phytobiomes Journal.2020; 4(2): 103. CrossRef
Tomato-Associated Archaea Show a Cultivar-Specific Rhizosphere Effect but an Unspecific Transmission by Seeds
Julian Taffner, Alessandro Bergna, Tomislav Cernava, Gabriele Berg
Phytobiomes Journal.2020; 4(2): 133. CrossRef
Influence of dragon bamboo with different planting patterns on microbial community and physicochemical property of soil on sunny and shady slopes
Weiyi Liu, Fang Wang, Yanmei Sun, Lei Yang, Huihai Chen, Weijie Liu, Bin Zhu, Chaomao Hui, Shiwei Wang
Journal of Microbiology.2020; 58(11): 906. CrossRef

First
Prev
Page of 1
Next
Last

Search