Journal of Microbiology

Restriction and Transcription Maps of Mitochondrial DNA of Trimorphomyces papilionaceus: Jeoung, Won Jin , Hong, Soon Gyu , Kang, Young Won , Jung, Hack Sung; J. Microbiol. 1995;33(2):149-153.

Abstract: Mitochondrial DNA has been isolated from Trimorphomyces papilionaceus. By analyzing DNA fragments digested by restriction enzymes, a restriction site map has been constructured. The mtDNA of T. papilionaceus amounts to 48.5 kb in size and is circular in structure. Entire mitochondrial DNA was cloned in E coli plasmids and Northern blot hybridization was done using cloned and subcloned DNAs as probes. Based on hybridization results of mitochondrial RNA transcripts, a transcription map was prepared.

Cloning and expression of pseudomonas cepacia catB gene in pseudomonas putida: Song, Seung Yeon , Jung, Young Hee , Lee, Myeong Sok , Lee, Ki Sung , Kim, Young Soo , Kim, Chi Kyung , Choi, Sang Ho , Min, Kyung Hee; J. Microbiol. 1996;34(4):334-340.

Abstract: The enzyme, cis,cis-muconate lactonizing enzyme has been proposed to play a key role in the β-ketoadipate pathway of benzoate degradation. A 3.2-kb EcoRI fragment termed as pRSU2, isolated from a Pseudomonas cepacia genomic library was able to complement the catB defective mutant. Several relevant restriction enzyme sites were determined within the cloned fragment. In Pseudomonas putida SUC2 carrying pRSU2, the enzyme activity was relatively higher than those of the induced or partially induced state of wild type P. putida PRS2000. It was probably due to higher expression of P. cepacia catB in P. putida PRS2000. It was probably due to higher expression of P. cepacia catB in P. putida. One possible interpretation of these results is that the catB promoter in P. cepacia is recognized within P. putida, resulting in the almost same expression level.

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