MSK
Search
- Page Path
- HOME > Search
Journal Article
- LAMMER Kinase Governs the Expression and Cellular Localization of Gas2, a Key Regulator of Flocculation in Schizosaccharomyces pombe
- Won-Hwa Kang , Yoon-Dong Park , Joo-Yeon Lim , Hee-Moon Park
- J. Microbiol. 2024;62(1):21-31. Published online January 5, 2024
- DOI: https://doi.org/10.1007/s12275-023-00097-7
- 66 View
- 0 Download
-
Abstract
- It was reported that LAMMER kinase in Schizosaccharomyces pombe plays an important role in cation-dependent and galactose-specific flocculation. Analogous to other flocculating yeasts, when cell wall extracts of the Δlkh1 strain were treated to the wild-type strain, it displayed flocculation. Gas2, a 1,3-β-glucanosyl transferase, was isolated from the EDTA-extracted cell-surface proteins in the Δlkh1 strain. While disruption of the gas2+ gene was not lethal and reduced the flocculation activity of the Δlkh1 strain, the expression of a secreted form of Gas2, in which the GPI anchor addition sequences had been removed, conferred the ability to flocculate upon the WT strain. The Gas2-mediated flocculation was strongly inhibited by galactose but not by glucose. Immunostaining analysis showed that the cell surface localization of Gas2 was crucial for the flocculation of fission yeast. In addition, we identified the regulation of mbx2+ expression by Lkh1 using RT-qPCR. Taken together, we found that Lkh1 induces asexual flocculation by regulating not only the localization of Gas2 but also the transcription of gas2+ through Mbx2.
Research Support, Non-U.S. Gov't
- Directed analysis of cyanobacterial membrane phosphoproteome using stained phosphoproteins and titanium-enriched phosphopeptides§
- Dong-Gi Lee , Joseph Kwon , Chi-Yong Eom , Young-Moon Kang , Seong Woon Roh , Kyung-Bok Lee , Jong-Soon Choi
- J. Microbiol. 2015;53(4):279-287. Published online April 8, 2015
- DOI: https://doi.org/10.1007/s12275-015-5021-8
- 54 View
- 0 Download
- 13 Crossref
-
Abstract
- Gel-free shotgun phosphoproteomics of unicellular cyanobacterium Synechocystis sp. PCC 6803 has not been reported up to now. The purpose of this study is to develop directed membrane phosphoproteomic method in Synechocystis sp. Total Synechocystis membrane proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and phosphoprotein-stained gel bands were selectively subjected to in-gel trypsin digestion. The phosphorylation sites of the resulting peptides were determined by assigning the neutral loss of [M-H3PO4] to Ser, Thr, and Tyr residues using nano-liquid chromatography 7 Tesla Fourier transform mass spectrometry. As an initial application, 111 proteins and 33 phosphoproteins were identified containing 11 integral membrane proteins. Identified four unknown phosphoproteins with transmembrane helices were suggested to be involved in membrane migration or transporters based on BLASTP search annotations. The overall distribution of hydrophobic amino acids in pTyr was lower in frequency than that of pSer or pThr. Positively charged amino acids were abundantly revealed in the surrounding amino acids centered on pTyr. A directed shotgun membrane phosphoproteomic strategy provided insight into understanding the fundamental regulatory processes underlying Ser, Thr, and Tyr phosphorylation in multi-layered membranous cyanobacteria.
-
Citations
Citations to this article as recorded by
- Global Profiling of Protein Phosphorylation, Acetylation, and β-Hydroxybutyrylation in Nannochloropsis oceanica
Lingyu Ouyang, Wuxin You, Ansgar Poetsch, Li Wei
Journal of Agricultural and Food Chemistry.2024; 72(47): 26248. CrossRef - Post-translational Modifications of Serine/Threonine and Histidine Kinases and Their Roles in Signal Transductions in Synechocystis Sp. PCC 6803
Wu Xu, Yingchun Wang
Applied Biochemistry and Biotechnology.2021; 193(3): 687. CrossRef - Comprehensive Phosphoproteomic Analysis of Nostoc flagelliforme in Response to Dehydration Provides Insights into Plant ROS Signaling Transduction
Wenyu Liang, Fengkun Yan, Meng Wang, Xiaoxu Li, Zheng Zhang, Xiaorong Ma, Jinhong Hu, Jun Wang, Lingxia Wang
ACS Omega.2021; 6(21): 13554. CrossRef - Metaproteomic analysis of harmful algal bloom in the Daechung reservoir, Korea
Jong-Soon Choi, Yun Hwan Park, Soo Hyeon Kim, Ju Seong Park, Yoon-E Choi
Environmental Biology Research.2020; 38(3): 424. CrossRef - Efficient profiling of detergent-assisted membrane proteome in cyanobacteria
Jong-Soon Choi, Yun Hwan Park, Jeong Hyun Oh, Sooyong Kim, Joseph Kwon, Yoon-E Choi
Journal of Applied Phycology.2020; 32(2): 1177. CrossRef - Assessment of Protein Content and Phosphorylation Level in Synechocystis sp. PCC 6803 under Various Growth Conditions Using Quantitative Phosphoproteomic Analysis
Masakazu Toyoshima, Yuma Tokumaru, Fumio Matsuda, Hiroshi Shimizu
Molecules.2020; 25(16): 3582. CrossRef - Proteomic De-Regulation in Cyanobacteria in Response to Abiotic Stresses
Piyoosh Kumar Babele, Jay Kumar, Venkatesh Chaturvedi
Frontiers in Microbiology.2019;[Epub] CrossRef - The S-layer biogenesis system of Synechocystis 6803: Role of Sll1180 and Sll1181 (E. coli HlyB and HlyD analogs) as type-I secretion components for Sll1951 export
Rachna Agarwal, Julian P. Whitelegge, Sanjay Saini, Amit Prakash Shrivastav
Biochimica et Biophysica Acta (BBA) - Biomembranes.2018; 1860(7): 1436. CrossRef - Proteomics of cyanobacteria: current horizons
Natalia Battchikova, Dorota Muth-Pawlak, Eva-Mari Aro
Current Opinion in Biotechnology.2018; 54: 65. CrossRef - Determination of the Role of Microcystis aeruginosa in Toxin Generation Based on Phosphoproteomic Profiles
Jiangqi Qu, Liping Shen, Meng Zhao, Wentong Li, Chengxia Jia, Hua Zhu, Qingjing Zhang
Toxins.2018; 10(7): 304. CrossRef - Functional Diversity of Transcriptional Regulators in the Cyanobacterium Synechocystis sp. PCC 6803
Mengliang Shi, Xiaoqing Zhang, Guangsheng Pei, Lei Chen, Weiwen Zhang
Frontiers in Microbiology.2017;[Epub] CrossRef - Proteomic analysis of post translational modifications in cyanobacteria
Qian Xiong, Zhuo Chen, Feng Ge
Journal of Proteomics.2016; 134: 57. CrossRef - Proteomic and metabolomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in cyanobacterium Synechocystis sp. PCC 6803
Yunpeng Wang, Lei Chen, Weiwen Zhang
Biotechnology for Biofuels.2016;[Epub] CrossRef
- Global Profiling of Protein Phosphorylation, Acetylation, and β-Hydroxybutyrylation in Nannochloropsis oceanica
- Purification and Properties of Novel Calcium-binding Proteins from Streptomyces coelicolor
- Chang, Ji Hun , Yoon, Soon Sang , Lhee, Sang Moon , Park, I Ha , Jung, Do Young , Park, Yong Sik , Yim, Jeong Bin
- J. Microbiol. 1999;37(1):21-26.
- 39 View
- 0 Download
-
Abstract
- Two novel calcium-binding proteins, named CAB-I and CAB-II, have been isolated from Streptomyces coelicolor. Purification of the calcium-binding proteins involved heat treatment, fractionation with ammonium sulfate, acid treatment, anion exchange and hydrophobic interaction column chromatography, FPLC gel filtration, and preparative isoelectric focusing. A chelex competitive assay and ^45Ca autoradiography verified the calcium-binding ability of the proteins. The major band CAB-II has an apparent molecular weight of 26,000 determined by SDS-polyacrylamide gel electrophoresis and 340,000 determined by gel filtration. The isoelectric point of this molecule showed the acidic nature of the molecule. N-terminal amino acid sequence analysis shows homology to rat Ca^2+/calmodulin-dependent protein kinase-II (CAB-II) and yeast phosphoprotein phosphatase (CAB-I).
First
Prev
TOP
