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- Bacillus ferrooxidans sp. nov., an iron(II)-oxidizing bacterium isolated from paddy soil
- Guo-Wei Zhou , Xiao-Ru Yang , Jian-Qiang Su , Bang-Xiao Zheng , Yong-Guan Zhu
- J. Microbiol. 2018;56(7):472-477. Published online June 14, 2018
- DOI: https://doi.org/10.1007/s12275-018-7543-3
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Abstract
- An endospore-forming bacterium, designated YT-3T, was isolated from a paddy soil in Yingtan, Jiangxi, China. Cells of strain YT-3T were Gram-positive, rod-shaped, facultative anaerobic, catalase, and oxidase positive. The optimum growth temperature and pH were 30°C (ranged from 15 to 50°C) and 6.5–7.0 (ranged from 3 to 11), respectively. Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%). The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was 80.4%, 81.5%, and 82.1%, respectively. The genomic DNA G + C content was 44.9 mol%. The predominant respiratory quinone was Menaquinone-7, and meso-diaminopimelic acid was present in the peptidoglycan layer of cell wall. The major fatty acids were C15:0 anteiso (36.2%), C14:0 iso (19.6%), C15:0 iso (17.4%), and C16:0 iso (9.8%). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipids, and ammoniac phospholipids. The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively. On the basis of physiological, genetic and biochemical data, strain YT-3T represented a novel species of the genus Bacillus, for which the name Bacillus ferrooxidans sp. nov was proposed. The type strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T).
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- Discarded masks as hotspots of antibiotic resistance genes during COVID-19 pandemic
Shu-Yi-Dan Zhou, Chenshuo Lin, Kai Yang, Le-Yang Yang, Xiao-Ru Yang, Fu-Yi Huang, Roy Neilson, Jian-Qiang Su, Yong-Guan Zhu
Journal of Hazardous Materials.2022; 425: 127774. CrossRef - Underestimation about the Contribution of Nitrate Reducers to Iron Cycling Indicated by Enterobacter Strain
Ming-Jun Li, Meng-Yun Wei, Xiao-Ting Fan, Guo-Wei Zhou
Molecules.2022; 27(17): 5581. CrossRef - Fe toxicity in plants: Impacts and remediation
Noreen Zahra, Muhammad Bilal Hafeez, Kanval Shaukat, Abdul Wahid, Mirza Hasanuzzaman
Physiologia Plantarum.2021;[Epub] CrossRef - Metabolic Inactivity and Re-awakening of a Nitrate Reduction Dependent Iron(II)-Oxidizing Bacterium Bacillus ferrooxidans
Guo-Wei Zhou, Xiao-Ru Yang, Regin Rønn, Jian-Qiang Su, Li Cui, Bang-Xiao Zheng, Yong-Guan Zhu
Frontiers in Microbiology.2019;[Epub] CrossRef
- Discarded masks as hotspots of antibiotic resistance genes during COVID-19 pandemic
- Isolation and Characterization of 2,4-Dichlorophenoxyacetic Acid-degrading Bacteria from Paddy Soils
- Chung, Min Jae , Ka, Jong Ok
- J. Microbiol. 1998;36(4):256-261.
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Abstract
- Nine numerically dominant 2,4-dichlorophenocyacetic acid (2,4-D)-degrading bacteria were isolated from rice field soils. Most of the isolates were identified as Brukholderia of Sphingomonas species by fatty acid methyl ester (FAME) analysis, and they exhibited diverse chromosomal DNA patterns in polymerase chain reaction (PCR)amplification of repetitive extragenic palindromic (REP) sequences. The isolates utilized 2,4-D as the sole source of carbon and two Sphingomonas species were capable of mineralizing both 3-chlorobenzoate (3-CB) and 4-chlorobenzoate (4-CB), in addition to 2,4-D. Plasmid DNAs were detected from all of the isolates, and conjugation analysis revealed that 2,4-D degradative genes were located on transferable plasmids in most of the isolates. PCR analysis with specific primers selected from tfd genes showed that 67% of the isolates had DNA sequences homologous to the five tfd genes of the 2,4-D degradative plasmid pJP4 of Alcaligenes eutrophus JMP134. Among the isolates, strain TFD7 appeared to be a new genotype in that it contained a transmissible 2,4-D degradative plasmid nonhomologous to the tfd genes. 2,4-D was persistent in natural paddy soils which contained no indigenous 2,4-D-degrading microorganisms, but the application of the 2,4-D-degrading isolates resulted in rapid decline of the soil 2,4-D residues.
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