Journal of Microbiology

Journal Articles

The efficacy of a 2,4-diaminoquinazoline compound as an intranasal vaccine adjuvant to protect against influenza A virus infection in vivo: Kyungseob Noh , Eun Ju Jeong , Timothy An , Jin Soo Shin , Hyejin Kim , Soo Bong Han , Meehyein Kim; J. Microbiol. 2022;60(5):550-559. Published online April 18, 2022; DOI: https://doi.org/10.1007/s12275-022-1661-7

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Adjuvants are substances added to vaccines to enhance antigen- specific immune responses or to protect antigens from rapid elimination. As pattern recognition receptors, Toll-like receptors 7 (TLR7) and 8 (TLR8) activate the innate immune system by sensing endosomal single-stranded RNA of RNA viruses. Here, we investigated if a 2,4-diaminoquinazolinebased TLR7/8 agonist, (S)-3-((2-amino-8-fluoroquinazolin- 4-yl)amino)hexan-1-ol (named compound 31), could be used as an adjuvant to enhance the serological and mucosal immunity of an inactivated influenza A virus vaccine. The compound induced the production of proinflammatory cytokines in macrophages. In a dose-response analysis, intranasal administration of 1 μg compound 31 together with an inactivated vaccine (0.5 μg) to mice not only enhanced virus-specific IgG and IgA production but also neutralized influenza A virus with statistical significance. Notably, in a virus-challenge model, the combination of the vaccine and compound 31 alleviated viral infection-mediated loss of body weight and increased survival rates by 40% compared with vaccine only-treated mice. We suggest that compound 31 is a promising lead compound for developing mucosal vaccine adjuvants to protect against respiratory RNA viruses such as influenza viruses and potentially coronaviruses.

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Efflux-Enhanced Imidazoquinolines To Exploit Chemoresistance
Muhammad Haroon, Sharmin Sultana, Seyedeh A. Najibi, Emily T. Wang, Abbey Michaelson, Pranto S. M. Al Muied, Amy E. Nielsen, Rock J. Mancini
ACS Omega.2025;[Epub] CrossRef
Design, Synthesis, and Biological Evaluation of New 2,6,7-Substituted Purine Derivatives as Toll-like Receptor 7 Agonists for Intranasal Vaccine Adjuvants
Morgan Kim, Kyungseob Noh, Pyeongkeun Kim, Jae Ho Kim, Byeong Wook Choi, Ravi Singh, Jun-Ho Choi, Soo Bong Han, Seong Soon Kim, Eun-Young Lee, Myung Ae Bae, Daeho Shin, Meehyein Kim, Jin Hee Ahn
Journal of Medicinal Chemistry.2024; 67(11): 9389. CrossRef
Evaluation of Antiviral Activity of Gemcitabine Derivatives against Influenza Virus and Severe Acute Respiratory Syndrome Coronavirus 2
Hyeon-Min Cha, Uk-Il Kim, Soo Bin Ahn, Myoung Kyu Lee, Haemi Lee, Hyungtae Bang, Yejin Jang, Seong Soon Kim, Myung Ae Bae, Kyungjin Kim, Meehyein Kim
ACS Infectious Diseases.2023; 9(4): 1033. CrossRef
Identification of broad-spectrum neutralizing antibodies against influenza A virus and evaluation of their prophylactic efficacy in mice
Sumin Son, Soo Bin Ahn, Geonyeong Kim, Yejin Jang, Chunkyu Ko, Meehyein Kim, Sang Jick Kim
Antiviral Research.2023; 213: 105591. CrossRef
Inhibition of KIF20A suppresses the replication of influenza A virus by inhibiting viral entry
Hoyeon Jeon, Younghyun Lim, In-Gu Lee, Dong-In Kim, Keun Pil Kim, So-Hee Hong, Jeongkyu Kim, Youn-Sang Jung, Young-Jin Seo
Journal of Microbiology.2022; 60(11): 1113. CrossRef

Antiviral effects of human placenta hydrolysate (Laennec) against SARS-CoV-2 in vitro and in the ferret model: Eun-Ha Kim , Young-il Kim , Seung-Gyu Jang , Minju Im , Kyeongsoo Jeong , Young Ki Choi , Hae-Jung Han; J. Microbiol. 2021;59(11):1056-1062. Published online October 6, 2021; DOI: https://doi.org/10.1007/s12275-021-1367-2

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Abstract

The COVID-19 pandemic has caused unprecedented health, social, and economic crises worldwide. However, to date, there is an only a limited effective treatment for this disease. Human placenta hydrolysate (hPH) has previously been shown to be safe and to improve the health condition in patients with hyperferritinemia and COVID-19. In this study, we aimed to determine the antiviral effects of hPH against SARS-CoV-2 in vitro and in vivo models and compared with Remdesivir, an FDA-approved drug for COVID-19 treatment. To assess whether hPH inhibited SARS-CoV-2 replication, we determined the CC50, EC50, and selective index (SI) in Vero cells by infection with a SARS-CoV-2 at an MOI of 0.01. Further, groups of ferrets infected with 105.8 TCID50/ml of SARS-CoV-2 and treated with hPH at 2, 4, 6 dpi, and compared their clinical manifestation and virus titers in respiratory tracts with PBS control-treated group. The mRNA expression of immunerelated cytokines was determined by qRT-PCR. hPH treatment attenuated virus replication in a dose-dependent manner in vitro. In a ferret infection study, treatment with hPH resulted in minimal bodyweight loss and attenuated virus replication in the nasal wash, turbinates, and lungs of infected ferrets. In addition, qRT-PCR results revealed that the hPH treatment remarkably upregulated the gene expression of type I (IFN-α and IFN-β) and II (IFN-γ) IFNs in SARS-CoV-2 infected ferrets. Our data collectively suggest that hPH has antiviral efficacy against SARS-CoV-2 and might be a promising therapeutic agent for the treatment of SARS-CoV-2 infection.

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Perinatal Hypoxia and Immune System Activation in Schizophrenia Pathogenesis: Critical Considerations During COVID-19 Pandemic
I Kawikova, K Hakenova, M Lebedeva, L Kleteckova, L Jakob, V Spicka, L Wen, F Spaniel, K Vales
Physiological Research.2024; : S615. CrossRef
Human Placenta Extract (HPH) Suppresses Inflammatory Responses in TNF-α/IFN-γ-Stimulated HaCaT Cells and a DNCB Atopic Dermatitis (AD)-Like Mouse Model
Jung Ok Lee, Youna Jang, A Yeon Park, Jung Min Lee, Kyeongsoo Jeong, So-Hyun Jeon, Hui Jin, Minju Im, Jae-Won Kim, Beom Joon Kim
Journal of Microbiology and Biotechnology.2024; 34(10): 1969. CrossRef
Systematic analysis of the pharmacology of standardized extracts of human placenta
T. E. Bogacheva, I. Yu. Torshin, O. A. Gromova
Pharmacokinetics and Pharmacodynamics.2024; (4): 3. CrossRef
Distinctive Combinations of RBD Mutations Contribute to Antibody Evasion in the Case of the SARS-CoV-2 Beta Variant
Tae-Hun Kim, Sojung Bae, Sunggeun Goo, Jinjong Myoung
Journal of Microbiology and Biotechnology.2023; 33(12): 1587. CrossRef
Current state-of-the-art and potential future therapeutic drugs against COVID-19
Ailong Sha, Yi Liu, Haiyan Hao
Frontiers in Cell and Developmental Biology.2023;[Epub] CrossRef
SARS-CoV-2 Aerosol and Intranasal Exposure Models in Ferrets
Elizabeth E. Zumbrun, Samantha E. Zak, Eric D. Lee, Philip A. Bowling, Sara I. Ruiz, Xiankun Zeng, Jeffrey W. Koehler, Korey L. Delp, Russel R. Bakken, Shannon S. Hentschel, Holly A. Bloomfield, Keersten M. Ricks, Tamara L. Clements, April M. Babka, John
Viruses.2023; 15(12): 2341. CrossRef
Human placenta hydrolysates: from V.P. Filatov to the present day: Review
Olga A. Gromova, Ivan Yu. Torshin, Alexander G. Chuchalin, Valeriy А. Maximov
Terapevticheskii arkhiv.2022; 94(3): 434. CrossRef

Characteristics of the gut microbiota colonization, inflammatory profile, and plasma metabolome in intrauterine growth restricted piglets during the first 12 hours after birth: Shimeng Huang , Na Li , Cong Liu , Tiantian Li , Wei Wang , Lili Jiang , Zhen Li , Dandan Han , Shiyu Tao , Junjun Wang; J. Microbiol. 2019;57(9):748-758. Published online June 11, 2019; DOI: https://doi.org/10.1007/s12275-019-8690-x

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Abstract

Intrauterine growth restriction (IUGR) predisposes newborns to inflammatory and metabolic disturbance. Disequilibrium of gut microbiota in early life has been implicated in the incidence of inflammation and metabolic diseases in adulthood. This study aimed to investigate the difference in gut microbiota colonization, cytokines and plasma metabolome between IUGR and normal birth weight (NBW) piglets in early life. At birth, reduced (P < 0.05) body, jejunum, and ileum weights, as well as decreased (P < 0.05) small intestinal villi and increased (P < 0.05) ileal crypt depth were observed in IUGR piglets compared with their NBW counterparts. Imbalanced inflammatory and plasma metabolome profile was observed in IUGR piglets. Furthermore, altered metabolites were mainly involved in fatty acid metabolism and inflammatory response. At 12 h after birth and after suckling colostrum, reduced (P < 0.05) postnatal growth and the small intestinal maturation retardation (P < 0.05) continued in IUGR piglets in comparison with those in NBW littermates. Besides, the gut microbiota structure was significantly altered by IUGR. Importantly, the disruption of the inflammatory profile and metabolic status mainly involved the pro-inflammatory cytokines (IL-1β and IFN-γ) and amino acid metabolism. Moreover, spearman correlation analysis showed that the increased abundance of Escherichia-Shigella and decreased abundance of Clostridium_sensu_stricto_1 in IUGR piglets was closely associated with the alterations of slaughter weight, intestinal morphology, inflammatory cytokines, and plasma metabolites. Collectively, IUGR significantly impairs small intestine structure, modifies gut microbiota colonization, and disturbs inflammatory and metabolic profiles during the first 12 h after birth. The unbalanced gut microbiota mediated by IUGR contributes to the development of inflammation and metabolic diseases.

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Endoplasmic Reticulum Stress Contributes to Intestinal Injury in Intrauterine Growth Restriction Newborn Piglets
Tingting Fang, Gang Tian, Daiwen Chen, Jun He, Ping Zheng, Xiangbing Mao, Hui Yan, Bing Yu
Animals.2024; 14(18): 2677. CrossRef
Characterization of the chemical composition of different parts of Dolichos lablab L. and revelation of its anti-ulcerative colitis effects by modulating the gut microbiota and host metabolism
Yanping Gao, Ruiting Huang, Yongyi Qiu, Yi Liu, Lei Chen
Journal of Ethnopharmacology.2024; 322: 117629. CrossRef
Plant-Derived Polysaccharides Benefit Weaned Piglets by Regulating Intestinal Microbiota: A Review
Xiaoyan Huang, Faming Jiang, Xingying Chen, Yuanhua Xian
Journal of Agricultural and Food Chemistry.2024; 72(51): 28225. CrossRef
Maternal Supplementation with Ornithine Promotes Placental Angiogenesis and Improves Intestinal Development of Suckling Piglets
Yun Yang, Guanyu Hou, Fengjie Ji, Hanlin Zhou, Renlong Lv, Chengjun Hu
Animals.2024; 14(5): 689. CrossRef
Dietary supplementation with dihydroartemisinin improves intestinal barrier function in weaned piglets with intrauterine growth retardation by modulating the gut microbiota
Yu Niu, Ruiqiang Zhang, Caimei Yang, Jintian He, Tian Wang
Journal of Animal Science.2024;[Epub] CrossRef
Phosphatidylethanolamine Improves Postnatal Growth Retardation by Regulating Mucus Secretion of Intestinal Goblet Cells in Piglets
Nan Wang, Chengming Wang, Ming Qi, Xingtong Lin, Andong Zha, Bie Tan, Yulong Yin, Jing Wang
Animals.2024; 14(8): 1193. CrossRef
Microbiota intestinal en recién nacidos prematuros con restricción del crecimiento intrauterino
Lizbeth Miranda-García, Francisco Josué Fernández-Becerra, Paulina Briseño-Sahagun
Revista Mexicana de Pediatría.2024; 91(2): 72. CrossRef
Gut microbiota-derived ursodeoxycholic acid alleviates low birth weight-induced colonic inflammation by enhancing M2 macrophage polarization
Yu Pi, Yujun Wu, Xiangyu Zhang, Dongdong Lu, Dandan Han, Jiangchao Zhao, Xiaojiao Zheng, Shiyi Zhang, Hao Ye, Shuai Lian, Yu Bai, Zhenyu Wang, Shiyu Tao, Dongjiao Ni, Xinhua Zou, Wei Jia, Guolong Zhang, Defa Li, Junjun Wang
Microbiome.2023;[Epub] CrossRef
Oil source regulates intestinal health of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatu): Structure, inflammation and microorganisms homeostasis
Xiaobo Yan, Hao Liu, Simiao Pan, Beiping Tan, Shuang Zhang, Hongyu Liu, Shuyan Chi, Qihui Yang, Yuanzhi Yang, Xiaohui Dong
Aquaculture.2023; 565: 739121. CrossRef
Dietary phytosterols supplementation improves the growth performance and decreases the abdominal fat of broiler chickens by regulating intestinal epithelial structure and microbiota
Hongjian Dai, Jian Gao, Yi Zhang, Haoze Wu, Yuqi Li, Zhanying Sun, Donghai Lv, Weiyun Zhu, Yanfen Cheng
Animal Feed Science and Technology.2023; 305: 115786. CrossRef
Gut Microbiota, Inflammation, and Probiotic Supplementation in Fetal Growth Restriction—A Comprehensive Review of Human and Animal Studies
Naser A. Alsharairi, Li Li
Life.2023; 13(12): 2239. CrossRef
Gut microbiota bridges dietary nutrients and host immunity
Lijuan Fan, Yaoyao Xia, Youxia Wang, Dandan Han, Yanli Liu, Jiahuan Li, Jie Fu, Leli Wang, Zhending Gan, Bingnan Liu, Jian Fu, Congrui Zhu, Zhenhua Wu, Jinbiao Zhao, Hui Han, Hao Wu, Yiwen He, Yulong Tang, Qingzhuo Zhang, Yibin Wang, Fan Zhang, Xin Zong,
Science China Life Sciences.2023; 66(11): 2466. CrossRef
Supplemental Clostridium butyricum modulates lipid metabolism by reshaping the gut microbiota composition and bile acid profile in IUGR suckling piglets
Xin Zhang, Yang Yun, Zheng Lai, Shuli Ji, Ge Yu, Zechen Xie, Hao Zhang, Xiang Zhong, Tian Wang, Lili Zhang
Journal of Animal Science and Biotechnology.2023;[Epub] CrossRef
Dietary bile acid supplementation in weaned piglets with intrauterine growth retardation improves colonic microbiota, metabolic activity, and epithelial function
Yang Liu, Md. Abul Kalam Azad, Sujuan Ding, Qian Zhu, Francois Blachier, Zugong Yu, Haijun Gao, Xiangfeng Kong
Journal of Animal Science and Biotechnology.2023;[Epub] CrossRef
Dousing the flame: reviewing the mechanisms of inflammatory programming during stress-induced intrauterine growth restriction and the potential for ω-3 polyunsaturated fatty acid intervention
Melanie R. White, Dustin T. Yates
Frontiers in Physiology.2023;[Epub] CrossRef
Metabolic Alternations During Gestation in Dezhou Donkeys and the Link to the Gut Microbiota
Yan Li, Qingshan Ma, Guiqin Liu, Zhenwei Zhang, Yandong Zhan, Mingxia Zhu, Changfa Wang
Frontiers in Microbiology.2022;[Epub] CrossRef
High-fat and high-protein diets from different sources induce different intestinal malodorous gases and inflammation
Tingting Zhang, Beizhen Xie, Hong Liu
Food Research International.2022; 154: 110989. CrossRef
The Exploration of Fetal Growth Restriction Based on Metabolomics: A Systematic Review
Mengxin Yao, Zhuoqiao Yang, Xin Rong, Xuan Hu, Na Yao, Manting Zhu, Xinnan Wang, Xiaoyan Zhu, Jieyun Yin
Metabolites.2022; 12(9): 860. CrossRef
Short-Chain Fatty Acids Modulate Permeability, Motility and Gene Expression in the Porcine Fetal Jejunum Ex Vivo
Barbara U. Metzler-Zebeli, Simone Koger, Suchitra Sharma, Arife Sener-Aydemir, Ursula Ruczizka, Heinrich Kreutzmann, Andrea Ladinig
Nutrients.2022; 14(12): 2524. CrossRef
Intrauterine Growth Retardation Affects Intestinal Health of Suckling Piglets via Altering Intestinal Antioxidant Capacity, Glucose Uptake, Tight Junction, and Immune Responses
Xiaopeng Tang, Kangning Xiong, Juan Gambini
Oxidative Medicine and Cellular Longevity.2022; 2022: 1. CrossRef
Intrauterine growth retardation affects liver bile acid metabolism in growing pigs: effects associated with the changes of colonic bile acid derivatives
Yang Liu, Md. Abul Kalam Azad, Wanghong Zhang, Liang Xiong, Francois Blachier, Zugong Yu, Xiangfeng Kong
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Unfavourable intrauterine environment contributes to abnormal gut microbiome and metabolome in twins
Jing Yang, Lingling Hou, Jinfeng Wang, Liwen Xiao, Jinyang Zhang, Nanlin Yin, Su Yao, Kun Cheng, Wen Zhang, Zhonghua Shi, Jing Wang, Hai Jiang, Nana Huang, Yanxia You, Mingmei Lin, Ruiyan Shang, Yuan Wei, Yangyu Zhao, Fangqing Zhao
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Uteroplacental Insufficiency Causes Microbiota Disruption and Lung Development Impairment in Growth-Restricted Newborn Rats
Yu-Chen S. H. Yang, Hsiu-Chu Chou, Yun-Ru Liu, Chung-Ming Chen
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Zinc Oxide Nanoparticle Improves the Intestinal Function of Intrauterine Growth Retardation Finishing Pigs via Regulating Intestinal Morphology, Inflammation, Antioxidant Status and Autophagy
Binbin Zhou, Jiaqi Zhang, Huijuan Liu, Shun Chen, Tian Wang, Chao Wang
Frontiers in Veterinary Science.2022;[Epub] CrossRef
The validation of the existence of the entero-mammary pathway and the assessment of the differences of the pathway between first and third parity sows
Laura L Greiner, Dalton C Humphrey, Shayla N Holland, C J Anderson, Stephan Schmitz-Esser
Translational Animal Science.2022;[Epub] CrossRef
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Xinzhi Tu, Chun Duan, Bingying Lin, Kangfeng Li, Jie Gao, Huaying Yan, Kejian Wang, Zhao Zhao
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Melatonin-induced changes in the bovine vaginal microbiota during maternal nutrient restriction
Riley D Messman, Zully E Contreras-Correa, Henry A Paz, Caleb O Lemley
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Drenching Bovine Colostrum, Quercetin or Fructo-Oligosaccharides Has No Effect on Health or Survival of Low Birth Weight Piglets
Kevin Van Tichelen, Sara Prims, Miriam Ayuso, Céline Van Kerschaver, Mario Vandaele, Jeroen Degroote, Steven Van Cruchten, Joris Michiels, Chris Van Ginneken
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Dietary dihydroartemisinin supplementation alleviates intestinal inflammatory injury through TLR4/NOD/NF-κB signaling pathway in weaned piglets with intrauterine growth retardation
Yu Niu, Yongwei Zhao, Jintian He, Yang Yun, Mingming Shen, Zhending Gan, Lili Zhang, Tian Wang
Animal Nutrition.2021; 7(3): 667. CrossRef
Effects of Bacillus subtilis on jejunal integrity, redox status, and microbial composition of intrauterine growth restriction suckling piglets
Yang Yun, Shuli Ji, Ge Yu, Peilu Jia, Yu Niu, Hao Zhang, Xin Zhang, Tian Wang, Lili Zhang
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Differences in Intestinal Barrier Development between Intrauterine Growth Restricted and Normal Birth Weight Piglets
Jarosław Olszewski, Romuald Zabielski, Tomasz Skrzypek, Piotr Matyba, Małgorzata Wierzbicka, Antoni Adamski, Elżbieta Grzesiuk, Maria Sady, Zdzisław Gajewski, Karolina Ferenc
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Clare H. Gaukroger, Christopher J. Stewart, Sandra A. Edwards, John Walshaw, Ian P. Adams, Ilias Kyriazakis
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Immunological Role of the Maternal Uterine Microbiome in Pregnancy: Pregnancies Pathologies and Alterated Microbiota
Jonah Bardos, Desiree Fiorentino, Ryan E. Longman, Michael Paidas
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Color of Colon Content of Normal and Intrauterine Growth-Restricted Weaned Piglets Is Associated with Specific Microbial Taxa and Physiological Parameters
Maria Wiese, Yan Hui, Dennis S. Nielsen, Andrew R. Williams, Julie C. Lynegaard, Nicolai R. Weber, Charlotte Amdi
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The Human Breast Milk Metabolome in Preeclampsia, Gestational Diabetes, and Intrauterine Growth Restriction: Implications for Child Growth and Development
Flaminia Bardanzellu, Melania Puddu, Vassilios Fanos
The Journal of Pediatrics.2020; 221: S20. CrossRef
Are Larger Litters a Concern for Piglet Survival or An Effectively Manageable Trait?
Sophia A. Ward, Roy N. Kirkwood, Kate J. Plush
Animals.2020; 10(2): 309. CrossRef
Membrane proteomic analysis reveals the intestinal development is deteriorated by intrauterine growth restriction in piglets
Shimeng Huang, Cong Liu, Na Li, Zhenhua Wu, Tiantian Li, Dandan Han, Zhen Li, Jiangchao Zhao, Junjun Wang
Functional & Integrative Genomics.2020; 20(2): 277. CrossRef
How could metabolomics change pediatric health?
Flaminia Bardanzellu, Vassilios Fanos
Italian Journal of Pediatrics.2020;[Epub] CrossRef
Perturbation of the lipid metabolism and intestinal inflammation in growing pigs with low birth weight is associated with the alterations of gut microbiota
Shi-Meng Huang, Zhen-Hua Wu, Tian-Tian Li, Cong Liu, Dan-Dan Han, Shi-Yu Tao, Yu Pi, Na Li, Jun-Jun Wang
Science of The Total Environment.2020; 719: 137382. CrossRef
Intrauterine Growth Restriction: New Insight from the Metabolomic Approach
Elena Priante, Giovanna Verlato, Giuseppe Giordano, Matteo Stocchero, Silvia Visentin, Veronica Mardegan, Eugenio Baraldi
Metabolites.2019; 9(11): 267. CrossRef

NMR-based metabolomics reveals the metabolite profiles of Vibrio parahaemolyticus under ferric iron stimulation: Jun Zhou , Chenyang Lu , Dijun Zhang , Chennv Ma , Xiurong Su; J. Microbiol. 2017;55(8):628-634. Published online July 28, 2017; DOI: https://doi.org/10.1007/s12275-017-6551-z

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Abstract

Vibrio parahaemolyticus is a halophilic bacterium endemic to coastal areas, and its pathogenicity has caused widespread seafood poisoning. In our previous research, the protein expression of V. parahaemolyticus in Fe3+ medium was determined using isobaric tags for relative and absolute quantitation (iTRAQ). Here, nuclear magnetic resonance (NMR) was used to detect changes in the V. parahaemolyticus metabolome. NMR spectra were obtained using methanol-water extracts of intracellular metabolites from V. parahaemolyticus under various culture conditions, and 62 metabolites were identified, including serine, arginine, alanine, ornithine, tryptophan, glutamine, malate, NAD+, NADP+, oxypurinol, xanthosine, dCTP, uracil, thymine, hypoxanthine, and betaine. Among these, 21 metabolites were up-regulated after the stimulation of the cells by ferric iron, and 9 metabolites were down-regulated. These metabolites are involved in amino acid and protein synthesis, energy metabolism, DNA and RNA synthesis and osmolality. Based on these results, we conclude that Fe3+ influences the metabolite profiles of V. parahaemolyticus.

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