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- Metabolomic profiling reveals enrichment of cordycepin in senescence process of Cordyceps militaris fruit bodies
- Junsang Oh , Deok-Hyo Yoon , Bhushan Shrestha , Hyung-Kyoon Choi , Gi-Ho Sung
- J. Microbiol. 2019;57(1):54-63. Published online December 29, 2018
- DOI: https://doi.org/10.1007/s12275-019-8486-z
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- 23 Web of Science
- 25 Crossref
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Abstract
-
Cordyceps militaris is a species of Cordyceps that is classified
in the Cordycipitaceae family and is well known in East Asia
as a traditional medicinal mushroom. Its artificial fruit body
has been widely cultivated for commercial use in cosmetics,
functional food, and medicine. To explore the metabolites
associated with fruit body development, we conducted gas
chromatography mass spectrometry (GC-MS) analyses based
on developmental stage, which was divided into the growth
period (stage 1, stage 2, and stage 3) and aging period (stage
4). We detected 39 biochemical metabolites associated with
nucleotide, carbohydrate, and amino acid metabolism. Cordycepin,
one of the representative bioactive compounds in
C. militaris, was significantly enriched in stage 4 of aging period
and is associated with glucose accumulation. The accumulation
of cordycepin in stage 4 of aging period also seems
to be related to the glutamine and glutamic acid pathway. Our
results
also showed enrichment of other bioactive compounds such as mannitol and xylitol in stage 4 of aging period. Our metabolomic profiling based on the developmental stages of C. militaris is useful for exploring bioactive compounds (e.g., cordycepin, mannitol, GABA, and xylitol) that are enriched in stage 4 of aging period and understanding the biosynthetic mechanisms associated with cordycepin production. Through optimization of fruit body cultivation by selecting stage 4 of aging period as a harvesting time, our findings can be utilized in food and medical applications of C. militaris in future. -
Citations
Citations to this article as recorded by
- Light-Exposed Metabolic Responses of Cordyceps militaris through Transcriptome-Integrated Genome-Scale Modeling
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Core microbes in
Cordyceps militaris
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Li Luo, Fei Dai, Zhongshun Xu, Jingqiang Guan, Gangxiang Fei, Jiaojiao Qu, Min Yao, Yuan Xue, Yeming Zhou, Xiao Zou, Christina A. Cuomo
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Guangyao Li, Tongtong Jian, Xiaojin Liu, Qingtao Lv, Guoying Zhang, Jianya Ling
Molecules.2022; 27(21): 7365. CrossRef - Cordycepin production by a novel endophytic fungus Irpex lacteus CHG05 isolated from Cordyceps hawkesii Gray
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Frontiers in Bioscience-Landmark.2022;[Epub] CrossRef -
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International Journal of Food Properties.2022; 25(1): 477. CrossRef - Effect of Spirulina biomass residue on yield and cordycepin and adenosine production of Cordyceps militaris culture
Siriluck Iamtham, Adisak Kaewkam, Sermsiri Chanprame, Wanida Pan-utai
Bioresource Technology Reports.2022; 17: 100893. CrossRef - Enhancement of cordycepin production from Cordyceps militaris culture by epigenetic modification
Phongsakorn Kunhorm, Phongsakorn Chueaphromsri, Nipha Chaicharoenaudomrung, Parinya Noisa
Biotechnology Letters.2022; 44(4): 581. CrossRef - Improved Cordycepin Production by Cordyceps militaris KYL05 Using Casein Hydrolysate in Submerged Conditions
Soo Kweon Lee, Ju Hun Lee, Hyeong Ryeol Kim, Youngsang Chun, Ja Hyun Lee, Hah Young Yoo, Chulhwan Park, Seung Wook Kim
Biomolecules.2019; 9(9): 461. CrossRef - Cordyceps militaris polysaccharides exerted protective effects on diabetic nephropathy in mice via regulation of autophagy
Dan-Dan Chen, Rui Xu, Jiu-Yao Zhou, Jun-Qi Chen, Lin Wang, Xu-Sheng Liu, Chun-ling Liang, Bi-Hao Liu, Rui-Rui Lu, Jun-Biao Wu, Hua Lin
Food & Function.2019; 10(8): 5102. CrossRef
- Light-Exposed Metabolic Responses of Cordyceps militaris through Transcriptome-Integrated Genome-Scale Modeling
Research Support, Non-U.S. Gov'ts
- Cloning and Sequence Analysis of a Glyceraldehyde-3-phosphate Dehydrogenase Gene from Ganoderma lucidum
- Xu Fei , Ming Wen Zhao , Yu Xiang Li
- J. Microbiol. 2006;44(5):515-522.
- DOI: https://doi.org/2446 [pii]
- 44 View
- 0 Download
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Abstract
- A cDNA library of Ganoderma lucidum has been constructed using a Zap Express cloning vector. A glyceraldehyde-3-phosphate dehydrogenase gene (gpd) was isolated from this library by hybridization of the recombinant phage clones with a gpd-specific gene probe generated by PCR. By comparison of the cDNA and the genomic DNA sequences, it was found that the complete nucleotide sequence encodes a putative polypeptide chain of 338 amino acids interrupted by 6 introns. The predicted amino acid sequence of this gene shows a high degree of sequence similarity to the GPD proteins from yeast and filamentous fungi. The promoter region contains a CT-rich stretch, two CAAT boxes, and a consensus TATA box. The possibility of using the gpd promoter in the construction of new transformation vectors is discussed.
- Identification of Medicinal Mushroom Species Based on Nuclear Large Subunit rDNA Sequences
- Ji Seon Lee , Mi Ok Lim , Kyoung Yeh Cho , Jung Hee Cho , Seung Yeup Chang , Doo Hyun Nam
- J. Microbiol. 2006;44(1):29-34.
- DOI: https://doi.org/2340 [pii]
- 40 View
- 0 Download
-
Abstract
- The purpose of this study was to develop molecular identification method for medical mushrooms and their preparations based on the nucleotide sequences of nuclear large subunit (LSU) rDNA. Four specimens were collected of each of the three representative medicinal mushrooms used in Korea: Ganoderma lucidum, Coriolus versicolor, and Fomes fomentarius. Fungal material used in these experiments included two different mycelial cultures and two different fruiting bodies from wild or cultivated mushrooms. The genomic DNA of mushrooms were extracted and 3 nuclear LSU rDNA fragments were amplified: set 1 for the 1.1-kb DNA fragment in the upstream region, set 2 for the 1.2-kb fragment in the middle, and set 3 for the 1.3-kb fragment downstream. The amplified gene products of nuclear large subunit rDNA from 3 different mushrooms were cloned into E. coli vector and subjected to nucleotide sequence determination. The sequence thus determined revealed that the gene sequences of the same medicinal mushroom species were more than 99.48% homologous, and the consensus sequences of 3 different medicinal mushrooms were more than 97.80% homologous. Restriction analysis revealed no useful restriction sites for 6-bp recognition enzymes for distinguishing the 3 sequences from one another, but some distinctive restriction patterns were recognized by the 4-bp recognition enzymes AccII and HhaI. This analysis was also confirmed by PCR-RFLP experiments on medicinal mushrooms.
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