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- Lithium Inhibits Growth of Intracellular Mycobacterium kansasii through Enhancement of Macrophage Apoptosis
- Hosung Sohn , Kwangwook Kim , Kil-Soo Lee , Han-Gyu Choi , Kang-In Lee , A-Rum Shin , Jong-Seok Kim , Sung Jae Shin , Chang-Hwa Song , Jeong-Kyu Park , Hwa-Jung Kim
- J. Microbiol. 2014;52(4):299-306. Published online February 17, 2014
- DOI: https://doi.org/10.1007/s12275-014-3469-6
- 64 View
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- 7 Crossref
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Abstract
- Mycobacterium kansasii (Mk) is an emerging pathogen that causes a pulmonary disease similar to tuberculosis. Macrophage apoptosis contributes to innate host defense against mycobacterial infection. Recent studies have suggested that lithium significantly enhances the cytotoxic activity of death stimuli in many cell types. We examined the effect of lithium on the viability of host cells and intracellular Mk in infected macrophages. Lithium treatment resulted in a substantial reduction in the viability of intracellular Mk in macrophages. Macrophage cell death was significantly enhanced after adding lithium to Mk-infected cells but not after adding to uninfected macrophages. Lithium-enhanced cell death was due to an apoptotic response, as evidenced by augmented DNA fragmentation and caspase activation. Reactive oxygen species were essential for lithium-induced apoptosis. Intracellular scavenging by N-acetylcysteine abrogated the lithiummediated decrease in intracellular Mk growth as well as apoptosis. These data suggest that lithium is associated with control of intracellular Mk growth through modulation of the apoptotic response in infected macrophages.
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Citations
Citations to this article as recorded by
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Recombinant Rv0753c Protein of Mycobacterium tuberculosis Induces Apoptosis Through Reactive Oxygen Species-JNK Pathway in Macrophages
Kang-In Lee, Seunga Choi, Han-Gyu Choi, Sintayehu Gurmessa Kebede, Thi Binh Dang, Yong Woo Back, Hye-Soo Park, Hwa-Jung Kim
Journal of Bacteriology and Virology.2020; 50(4): 246. CrossRef - Investigating the Role of Everolimus in mTOR Inhibition and Autophagy Promotion as a Potential Host-Directed Therapeutic Target in Mycobacterium tuberculosis Infection
Stephen Cerni, Dylan Shafer, Kimberly To, Vishwanath Venketaraman
Journal of Clinical Medicine.2019; 8(2): 232. CrossRef - Mycobacterium abscessus glycopeptidolipids inhibit macrophage apoptosis and bacterial spreading by targeting mitochondrial cyclophilin D
Jake Whang, Yong Woo Back, Kang-In Lee, Nagatoshi Fujiwara, Seungwha Paik, Chul Hee Choi, Jeong-Kyu Park, Hwa-Jung Kim
Cell Death & Disease.2017; 8(8): e3012. CrossRef - Invasion of Mammalian Cells by Rough Variant ofMycobacterium abscessus
Jake Whang, Young Woo Back, Gang-In Lee, Hwa-Jung Kim
Journal of Bacteriology and Virology.2016; 46(4): 193. CrossRef - Mycobacterium tuberculosis effectors interfering host apoptosis signaling
Minqiang Liu, Wu Li, Xiaohong Xiang, Jianping Xie
Apoptosis.2015; 20(7): 883. CrossRef - Targeting Batf2 for infectious diseases and cancer
Reto Guler, Sugata Roy, Harukazu Suzuki, Frank Brombacher
Oncotarget.2015; 6(29): 26575. CrossRef - Extended stability of cyclin D1 contributes to limited cell cycle arrest at G1-phase in BHK-21 cells with Japanese encephalitis virus persistent infection
Ji Young Kim, Soo Young Park, Hey Rhyoung Lyoo, Eung Seo Koo, Man Su Kim, Yong Seok Jeong
Journal of Microbiology.2015; 53(1): 77. CrossRef
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Recombinant Rv0753c Protein of Mycobacterium tuberculosis Induces Apoptosis Through Reactive Oxygen Species-JNK Pathway in Macrophages
Journal Article
- High Efficiency Transformation by Electroporation of Yarrowia lipolytica
- Jia-Hung Wang , Wenpin Hung , Shu-Hsien Tsai
- J. Microbiol. 2011;49(3):469-472. Published online June 30, 2011
- DOI: https://doi.org/10.1007/s12275-011-0433-6
- 38 View
- 0 Download
- 36 Crossref
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Abstract
- Yarrowia lipolytica was usually transformed by heat shock, but linearized integrative vectors always resulted in a low transformation efficiency when electroporation was used. To develop a high efficiency integrative transformation method by electroporation of Y. lipolytica, we report here that pretreatment of Y. lipolytica with 150 mM LiAc for 1 h before electroporation will approximately 30-fold of increase transformation efficiency. A cell concentration of 1010/ml and instrument settings of 1.5 kV will generate the highest transformation efficiencies. We have developed a procedure to transform Y. lipolytica that will be able to yield an efficiency of 2.1×104 transformants/μg for integrative linear DNA. With our modifications, the electroporation procedures became a very efficient and reliable tool for Y. lipolytica transformation.
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Citations
Citations to this article as recorded by- Advances in synthetic biology tools paving the way for the biomanufacturing of unusual fatty acids using the Yarrowia lipolytica chassis
Kaifeng Wang, Tian-Qiong Shi, Lu Lin, Ping Wei, Rodrigo Ledesma-Amaro, Xiao-Jun Ji, He Huang
Biotechnology Advances.2022; 59: 107984. CrossRef - Orotic acid production by Yarrowia lipolytica under conditions of limited pyrimidine
Paul Swietalski, Frank Hetzel, Iris Klaiber, Eva Pross, Ines Seitl, Lutz Fischer
Yeast.2022; 39(3): 230. CrossRef - Recent advances in genetic technology development of oleaginous yeasts
Zhiqiang Wen, Naief H. Al Makishah
Applied Microbiology and Biotechnology.2022; 106(17): 5385. CrossRef - Identification and Characterization of the Mitochondrial Replication Origin for Stable and Episomal Expression in Yarrowia lipolytica
Zhiyong Cui, Huihui Zheng, Zhennan Jiang, Zhaoxuan Wang, Jin Hou, Qian Wang, Quanfeng Liang, Qingsheng Qi
ACS Synthetic Biology.2021; 10(4): 826. CrossRef - Recent advances in lipid metabolic engineering of oleaginous yeasts
Atrayee Chattopadhyay, Mohor Mitra, Mrinal K. Maiti
Biotechnology Advances.2021; 53: 107722. CrossRef - Yarrowia lipolytica Strains and Their Biotechnological Applications: How Natural Biodiversity and Metabolic Engineering Could Contribute to Cell Factories Improvement
Catherine Madzak
Journal of Fungi.2021; 7(7): 548. CrossRef - Revisiting the unique structure of autonomously replicating sequences in Yarrowia lipolytica and its role in pathway engineering
Carmen Lopez, Mingfeng Cao, Zhanyi Yao, Zengyi Shao
Applied Microbiology and Biotechnology.2021; 105(14-15): 5959. CrossRef - Optimization of electrotransformation (ETF) conditions in lactic acid bacteria (LAB)
Chao Wang, Yanhua Cui, Xiaojun Qu
Journal of Microbiological Methods.2020; 174: 105944. CrossRef - Understanding and Eliminating the Detrimental Effect of Thiamine Deficiency on the Oleaginous Yeast Yarrowia lipolytica
Caleb Walker, Seunghyun Ryu, Richard J. Giannone, Sergio Garcia, Cong T. Trinh, Haruyuki Atomi
Applied and Environmental Microbiology.2020;[Epub] CrossRef - Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolytica
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MicrobiologyOpen.2020;[Epub] CrossRef - Secretion of a low and high molecular weight β-glycosidase by Yarrowia lipolytica
Paul Swietalski, Frank Hetzel, Ines Seitl, Lutz Fischer
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Seunghyun Ryu, Cong T. Trinh, Marie A. Elliot
Applied and Environmental Microbiology.2018;[Epub] CrossRef - Metabolic engineering in the host Yarrowia lipolytica
Ahmad M. Abdel-Mawgoud, Kelly A. Markham, Claire M. Palmer, Nian Liu, Gregory Stephanopoulos, Hal S. Alper
Metabolic Engineering.2018; 50: 192. CrossRef - Optimization of chemico-physical transformation methods for various bacterial species using diverse chemical compounds and nanomaterials
Jun Ren, Dokyun Na, Seung Min Yoo
Journal of Biotechnology.2018; 288: 55. CrossRef - Recent Advances in Metabolic Engineering of Yarrowia lipolytica for Lipid Overproduction
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FEMS Yeast Research.2018;[Epub] CrossRef - Synthesis and assembly of human vault particles in yeast
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Biotechnology and Bioengineering.2018; 115(12): 2941. CrossRef - Fast and efficient genetic transformation of oleaginous yeast Rhodosporidium toruloides by using electroporation
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Applied Biochemistry and Biotechnology.2016; 180(8): 1497. CrossRef - Recent advances in bioengineering of the oleaginous yeast Yarrowia lipolytica
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Marina Miklenić, Bojan Žunar, Anamarija Štafa, Ivan-Krešimir Svetec, Jens Nielsen
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- Advances in synthetic biology tools paving the way for the biomanufacturing of unusual fatty acids using the Yarrowia lipolytica chassis
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