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- Isolation and Functional Characterization of a Delta 6-Desaturase Gene from the Pike Eel (Muraenesox cinereus)
- Sun Hee Kim , Kyung Hee Roh , Jung-Bong Kim , Kwang-Soo Kim , Nam Shin Kim , Hyun Uk Kim , Kyeong-Ryeol Lee , Jong-Sug Park , Jong-Bum Kim
- J. Microbiol. 2013;51(6):807-813. Published online October 5, 2013
- DOI: https://doi.org/10.1007/s12275-013-3144-3
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- 9 Crossref
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Abstract
-
Stearidonic acid (STA; 18:4n-3) and γ-linolenic acid (GLA;
18:3n-6) are significant intermediates in the biosynthetic pathway
for the very-long-chain polyunsaturated fatty acids of
eicosapentaenoic acid (EPA; 20:5n-3) and arachidonic acid
(ARA; 20:4n-6), respectively. To develop a sustainable system
for the production of dietary polyunsaturated fatty acids,
we focused on the action of the enzyme delta 6-desaturase
(D6DES) on the essential acids, linoleic acid (LA; 18:2n-6)
and α-linolenic acid (ALA; 18:3n-3). A 1,335-bp full-length
cDNA encoding D6DES (McD6DES) was cloned from Muraenesox
cinereus using degenerate PCR and RACE-PCR
methods
. To investigate the enzymatic activity of McD6DES in the production of n-6 and n-3 fatty acids, a recombinant plasmid expressing McD6DES (pYES-McD6DES) was transformed into and expressed in Saccharomyces cerevisiae. The exogenously expressed McD6DES produced GLA and STA at conversion rates of 14.2% and 45.9%, respectively, from the exogenous LA and ALA substrates. These results indicate that McD6DES is essentially a delta 6-desaturase involved in very-long-chain polyunsaturated fatty acid synthesis. -
Citations
Citations to this article as recorded by
- Regulation of long-chain polyunsaturated fatty acid biosynthesis in teleost fish
Dizhi Xie, Cuiying Chen, Yewei Dong, Cuihong You, Shuqi Wang, Óscar Monroig, Douglas R. Tocher, Yuanyou Li
Progress in Lipid Research.2021; 82: 101095. CrossRef - Comparative and functional analysis of desaturase FADS1 (∆5) and FADS2 (∆6) orthologues of marine organisms
Crisalejandra Rivera-Pérez, Fausto Valenzuela-Quiñonez, Javier Caraveo-Patiño
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics.2020; 35: 100704. CrossRef - Δ6 fatty acid desaturases in polyunsaturated fatty acid biosynthesis: insights into the evolution, function with substrate specificities and biotechnological use
Jie Cui, Haiqin Chen, Xin Tang, Jianxin Zhao, Hao Zhang, Yong Q. Chen, Wei Chen
Applied Microbiology and Biotechnology.2020; 104(23): 9947. CrossRef - In Silico Structural Studies and Molecular Docking Analysis of Delta6-desaturase in HUFA Biosynthetic Pathway
Suvra Roy, Hirak jyoti Chakraborty, Vikash Kumar, B K Behera, R S Rana, Gireesh Babu
Animal Biotechnology.2018; 29(3): 161. CrossRef - Changes in Plasma and Tissue Long-Chain Polyunsaturated Fatty Acid (LC-PUFA) Content in the Eel Anguilla japonica After External and Internal Osmotic Stress
Qinghao Zhang, Marty K. S. Wong, Yiqi Li, Yuanyou Li, Yoshio Takei
Zoological Science.2017; 34(5): 429. CrossRef - Effect of low temperature on highly unsaturated fatty acid biosynthesis in activated sludge
Su He, Li-li Ding, Ke Xu, Jin-ju Geng, Hong-qiang Ren
Bioresource Technology.2016; 211: 494. CrossRef - Metabolic engineering to produce γ-linolenic acid in Brassica napus using a Δ6-desaturase from pike eel
Sun Hee Kim, Kyung Hee Roh, Kyeong-Ryeol Lee, Han-Chul Kang, Hyun Uk Kim, Jong Bum Kim
Plant Biotechnology Reports.2016; 10(6): 475. CrossRef - Heterologous Reconstitution of Omega-3 Polyunsaturated Fatty Acids inArabidopsis
Sun Hee Kim, Kyung Hee Roh, Jong-Sug Park, Kwang-Soo Kim, Hyun Uk Kim, Kyeong-Ryeol Lee, Han-Chul Kang, Jong-Bum Kim
BioMed Research International.2015; 2015: 1. CrossRef - Coexpression of multiple genes reconstitutes two pathways of very long-chain polyunsaturated fatty acid biosynthesis in Pichia pastoris
Sun Hee Kim, Kyung Hee Roh, Kwang-Soo Kim, Hyun Uk Kim, Kyeong-Ryeol Lee, Han-Chul Kang, Jong-Bum Kim
Biotechnology Letters.2014; 36(9): 1843. CrossRef
- Regulation of long-chain polyunsaturated fatty acid biosynthesis in teleost fish
Journal Article
- Effect of pH on Conjugated Linoleic Acid (CLA) Formation of Linolenic Acid Biohydrogenation by Ruminal Microorganisms
- Yongjae Lee
- J. Microbiol. 2013;51(4):471-476. Published online August 30, 2013
- DOI: https://doi.org/10.1007/s12275-013-1070-z
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- 0 Download
- 9 Crossref
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Abstract
- Conventional beliefs surrounding the linolenic acid (LNA; cis-9 cis-12 cis-15 C18:3) biohydrogenation (BH) pathway propose that it converts to stearic acid (SA) without the formation of conjugated linoleic acid (CLA) as intermediate isomers. However, an advanced study (Lee and Jenkins, 2011) verified that LNA BH yields multiple CLAs. This study utilized the stable isotope tracer to investigate the BH intermediates of 13C-LNA with different pH conditions (5.5 and 6.5). The 13C enrichment was calculated as a 13C/12C ratio of labeled minus unlabeled. After 24 h, eight CLA isomers were significantly enriched on both pH treatment, this result verifies that these CLAs originated from 13C-LNA BH which supports the results of Lee and Jenkins (2011). The enrichment of cis-cis double bond CLAs (cis-9 cis-11 and cis-10 cis-12 CLA) were significantly higher at low pH conditions. Furthermore, the concentration of cis-10 cis-12 CLA at low pH was four times higher than at high pH conditions after a 3 h incubation. These differences support the LNA BH pathways partial switch under different pH conditions, with a strong influence on the cis-cis CLA at low pH. Several mono-, di-, and tri-enoic fatty acid isomers were enriched during 24 h of incubation, but the enrichment was decreased or restricted at low pH treatment. Based on these results, it is proposed that low pH conditions may cause a changed or limited capacity of the isomerization and reduction steps in BH.
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Citations
Citations to this article as recorded by- Invited review: Research on ruminal biohydrogenation—Achievements, gaps in knowledge, and future approaches from the perspective of dairy science
P.G. Toral, G. Hervás, P. Frutos
Journal of Dairy Science.2024; 107(12): 10115. CrossRef - Selenate and selenite affect ruminal metabolism of C18 unsaturated fatty acids and fatty acid composition of lamb tissues
Małgorzata Białek, Marian Czauderna, Wiesław Przybylski, Danuta Jaworska
Livestock Science.2020; 241: 104249. CrossRef - Effects of Supplementation of Rumen Protected Fats on Rumen Ecology and Digestibility of Nutrients in Sheep
Atique A. Behan, Teck Chwen Loh, Sharida Fakurazi, Ubedullah Kaka, Asmatullah Kaka, Anjas Asmara Samsudin
Animals.2019; 9(7): 400. CrossRef - In vitro biohydrogenation of 13C-labeled α-linolenic acid in response to ruminal alterations associated with diet-induced milk fat depression in ewes
P.G. Toral, G. Hervás, P. Frutos
Journal of Dairy Science.2019; 102(2): 1213. CrossRef - Accumulation of conjugated linoleic acid in Lactobacillus plantarum WU-P19 is enhanced by induction with linoleic acid and chitosan treatment
Wilawan Palachum, Wanna Choorit, Yusuf Chisti
Annals of Microbiology.2018; 68(10): 611. CrossRef - The In vitro Effects of Nano-encapsulated Conjugated Linoleic Acid on Stability of Conjugated Linoleic Acid and Fermentation Profiles in the Rumen
Wan Heo, Eun Tae Kim, Sung Do Cho, Jun Ho Kim, Seong Min Kwon, Ha Yeon Jeong, Kwang Seok Ki, Ho Baek Yoon, Young Dae Ahn, Sung Sill Lee, Young Jun Kim
Asian-Australasian Journal of Animal Sciences.2016; 29(3): 365. CrossRef - Sources and Bioactive Properties of Conjugated Dietary Fatty Acids
Alan A. Hennessy, Paul R. Ross, Gerald F. Fitzgerald, Catherine Stanton
Lipids.2016; 51(4): 377. CrossRef - Influence of Carotino oil on in vitro rumen fermentation, metabolism and apparent biohydrogenation of fatty acids
Kazeem Dauda Adeyemi, Mahdi Ebrahimi, Anjas Asmara Samsudin, Abd Razak Alimon, Roselina Karim, Saiful Anuar Karsani, Awis Qurni Sazili
Animal Science Journal.2015; 86(3): 270. CrossRef - Seleno‐methionine decreases biohydrogenation of C18 unsaturated fatty acids in ovine ruminal fluid incubated in vitro with α‐linolenic acid
Marian Czauderna, Agnieszka J. Rozbicka‐Wieczorek, Edyta Więsyk, Katarzyna A. Krajewska‐Bienias
European Journal of Lipid Science and Technology.2015; 117(6): 820. CrossRef
- Invited review: Research on ruminal biohydrogenation—Achievements, gaps in knowledge, and future approaches from the perspective of dairy science
Research Support, Non-U.S. Gov't
- Molecular Cloning and Expression Analysis of a Delta 6-Fatty Acid Desaturase Gene from Rhizopus stolonifer Strain YF6 Which Can Accumulate High Levels of Gamma-Linolenic Acid
- Xia Wan , Yinbo Zhang , Ping Wang , Mulan Jiang
- J. Microbiol. 2011;49(1):151-154. Published online March 3, 2011
- DOI: https://doi.org/10.1007/s12275-011-0254-7
- 29 View
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- 10 Scopus
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Abstract
- The delta 6-desaturase gene was cloned from Rhizopus stolonifer, which could accumulate up to 49% of gamma-linolenic acid (GLA, C18:3 Δ6,9,12) to the total fatty acids. The cloned DNA contains a 1,380 bp open reading frame encoding a protein of 460 amino acids, which showed high similarity to those of fungal delta 6-desaturases with three conserved histidine-rich motifs and HPGG motif. Notably, this deduced sequence had a shorter C-terminus. Results demonstrated that the cDNA sequence exhibited delta 6-desaturase activity by accumulation of about 22.4% of GLA to the total fatty acids in the recombinant Pichia pastoris strain GS115.
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