The gut bacterial community of wood-feeding beetles has
been examined for its role on plant digestion and biocontrol method development. Monochamus alternatus and Psacothea
hilaris, both belonging to the subfamily Lamiinae, are woodfeeding
beetles found in eastern Asia and Europe and generally
considered as destructive pests for pine and mulberry
trees, respectively. However, limited reports exist on the gut
bacterial communities in these species. Here, we characterized
gut bacterial community compositions in larva and imago of
each insect species reared with host tree logs and artificial
diets as food sources. High-throughput 454 pyrosequencing
of bacterial 16S rRNA gene revealed 225 operational taxonomic
units (OTUs) based on a 97% sequences similarity cutoff
from 138,279 sequence reads, the majority of which were
derived from Proteobacteria (48.2%), Firmicutes (45.5%), and
Actinobacteria (5.2%). The OTU network analysis revealed
7 modules with densely connected OTUs in specific gut samples,
in which the distributions of Lactococcus-, Kluyvera-,
Serratia-, and Enterococcus-related OTUs were distinct between
diet types or developmental stages of the host insects.
The gut bacterial communities were separated on a detrended
correspondence analysis (DCA) plot and by c-means fuzzy
clustering analysis, according to diet type. The results from
this study suggest that diet was the main determinant for gut
bacterial community composition in the two beetles.
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