MSK
Search
- Page Path
- HOME > Search
Journal Articles
- Structural and Functional Analyses of the Flavoprotein Disulfide Reductase FN0820 of Fusobacterium nucleatum
- Hyunwoo Shin , Yeongjin Baek , Dukwon Lee , Yongbin Xu , Yonghoon Kwon , Inseong Jo , Nam-Chul Ha
- J. Microbiol. 2023;61(12):1033-1041. Published online December 20, 2023
- DOI: https://doi.org/10.1007/s12275-023-00095-9
- 541 View
- 1 Download
- 1 Web of Science
- 1 Crossref
-
Abstract
PDF - Escherichia coli RclA and Staphylococcus aureus MerA are part of the Group I flavoprotein disulfide reductase (FDR) family and have been implicated in the contribution to bacterial pathogenesis by defending against the host immune response. Fusobacterium nucleatum is a pathogenic, anaerobic Gram-negative bacterial species commonly found in the human oral cavity and gastrointestinal tract. In this study, we discovered that the F. nucleatum protein FN0820, belonging to the Group I FDR family, exhibited a higher activity of a Cu2+- dependent NADH oxidase than E. coli RclA. Moreover, FN0820 decreased the dissolved oxygen level in the solution with higher NADH oxidase activity. We found that L-tryptophan and its analog 5-hydroxytryptophan inhibit the FN0820 activities of NADH oxidase and the concomitant reduction of oxygen. Our results have implications for developing new treatment strategies against pathogens that defend the host immune response with Group I FDRs.
-
Citations
Citations to this article as recorded by
-
The role of metals in hypothiocyanite resistance in
Escherichia coli
Michael J. Gray, Laurie E. Comstock
Journal of Bacteriology.2024;[Epub] CrossRef
-
The role of metals in hypothiocyanite resistance in
Escherichia coli
- Salmonella Typhimurium ST313 isolated in Brazil revealed to be more invasive and inflammatory in murine colon compared to ST19 strains
- Amanda Aparecida Seribelli , Tamara R. Machado Ribeiro , Patrick da Silva† , Isabela Mancini Martins , Felipe Pinheiro Vilela , Marta I. Cazentini Medeiros , Kamila Chagas Peronni , Wilson Araújo da Silva Junior , Cristiano Gallina Moreira , Juliana Pfrimer Falcão
- J. Microbiol. 2021;59(9):861-870. Published online August 12, 2021
- DOI: https://doi.org/10.1007/s12275-021-1082-z
- 496 View
- 1 Download
- 7 Web of Science
- 7 Crossref
-
Abstract
PDF
- Salmonella Typhimurium (ST313) has caused an epidemic of invasive disease in sub-Saharan Africa and has been recently identified in Brazil. As the virulence of this ST is poorly understood, the present study aimed to (i) perform the RNAseq in vitro of S. Typhimurium STm30 (ST313) grown in Luria-Bertani medium at 37°C; (ii) compare it with the RNAseq of the S. Typhimurium SL1344 (ST19) and S. Typhimurium STm11 (ST19) strains under the same growing conditions; and (iii) examine the colonization capacity and expression of virulence genes and cytokines in murine colon. The STm30 (ST313) strain exhibited stronger virulence and was associated with a more inflammatory profile than the strains SL1344 (ST19) and STm11 (ST19), as demonstrated by transcriptome and in vivo assay. The expression levels of the hilA, sopD2, pipB, and ssaS virulence genes, other Salmonella pathogenicity islands SPI-1 and SPI-2 genes or effectors, and genes of the cytokines IL-1β, IFN-γ, TNF-α, IL-6, IL-17, IL-22, and IL-12 were increased during ST313 infection in C57BL/6J mice. In conclusion, S. Typhimurium STm30 (ST313) isolated from human feces in Brazil express higher levels of pathogenesis- related genes at 37°C and has stronger colonization and invasion capacity in murine colon due to its high expression levels of virulence genes, when compared with the S. Typhimurium SL1344 (ST19) and STm11 (ST19) strains. STm30 (ST313) also induces stronger expression of pro-inflammatory cytokines in this organ, suggesting that it causes more extensive tissue damage.
-
Citations
Citations to this article as recorded by- Inflammation, toxicity, and apoptosis reducing potential of bacteriophage Ariobarzanes on intestinal cells infected with Salmonella Typhimurium
Mina Rahiminejad, Maryam Montaseri, Mohammad Hashem Yousefi, Saeed Nazifi, Jeroen Wagemans, Saeid Hosseinzadeh
Scientific Reports.2025;[Epub] CrossRef - Whole-Genome Sequencing Reveals the Population Structure and Genetic Diversity of Salmonella Typhimurium ST34 and ST19 Lineages
Zhen-xu Zhuo, Yu-lian Feng, Xi-wei Zhang, Hao Liu, Fang-yin Zeng, Xiao-yan Li
Journal of Microbiology.2024; 62(10): 859. CrossRef -
Incremental increases in physiological fluid shear progressively alter pathogenic phenotypes and gene expression in multidrug resistant
Salmonella
Jiseon Yang, Jennifer Barrila, Eric A. Nauman, Seth D. Nydam, Shanshan Yang, Jin Park, Ami D. Gutierrez-Jensen, Christian L. Castro, C. Mark Ott, Kristina Buss, Jason Steel, Anne D. Zakrajsek, Mary M. Schuff, Cheryl A. Nickerson
Gut Microbes.2024;[Epub] CrossRef - Virulence potential of Salmonella 1,4, [5],12:i:- strains isolated during decades from different sources in the Southeast region of Brazil
Giovana do Nascimento Pereira, Amanda Aparecida Seribelli, Carolina Nogueira Gomes, Felipe Pinheiro Vilela, Ludmilla Tonani, Monique Ribeiro Tiba-Casas, Marta Inês Cazentini Medeiros, Dália dos Prazeres Rodrigues, Márcia Regina von Zeska Kress, Juliana Pf
Brazilian Journal of Microbiology.2023; 54(4): 2827. CrossRef - Invasive non-typhoidal Salmonella (iNTS) aminoglycoside-resistant ST313 isolates feature unique pathogenic mechanisms to reach the bloodstream
Isabela Mancini Martins, Amanda Aparecida Seribelli, Tamara R. Machado Ribeiro, Patrick da Silva, Bruna Cardinali Lustri, Rodrigo T. Hernandes, Juliana Pfrimer Falcão, Cristiano Gallina Moreira
Infection, Genetics and Evolution.2023; 116: 105519. CrossRef - Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Microbial Pathogenesis.2022; 165: 105460. CrossRef - Antimicrobial resistance and genetic background of non-typhoidal Salmonella enterica strains isolated from human infections in São Paulo, Brazil (2000–2019)
Aline Parolin Calarga, Marco Tulio Pardini Gontijo, Luiz Gonzaga Paula de Almeida, Ana Tereza Ribeiro de Vasconcelos, Leandro Costa Nascimento, Taíse Marongio Cotrim de Moraes Barbosa, Thalita Mara de Carvalho Perri, Silvia Regina dos Santos, Monique Ribe
Brazilian Journal of Microbiology.2022; 53(3): 1249. CrossRef
- Inflammation, toxicity, and apoptosis reducing potential of bacteriophage Ariobarzanes on intestinal cells infected with Salmonella Typhimurium
Research Support, Non-U.S. Gov't
- Comparative Genomic Analysis of Bacteriophage EP23 Infecting Shigella sonnei and Escherichia coli
- Ho-Won Chang , Kyoung-Ho Kim
- J. Microbiol. 2011;49(6):927-934. Published online December 28, 2011
- DOI: https://doi.org/10.1007/s12275-011-1577-0
- 244 View
- 0 Download
- 21 Crossref
-
Abstract
PDF
- Bacteriophage EP23 that infects Escherichia coli and Shigella sonnei was isolated and characterized. The bacteriophage morphology was similar to members of the family Siphoviridae. The 44,077 bp genome was fully sequenced using 454 pyrosequencing. Comparative genomic and phylogenetic analyses showed that EP23 was most closely related to phage SO-1, which infects Sodalis glossinidius and phage SSL-2009a, which infects engineered E. coli. Genomic comparison indicated that EP23 and SO-1 were very similar with each other in terms of gene order and amino acid similarity, even though their hosts were separated in the level of genus. EP23 and SSL-2009a displayed high amino acid similarity between their genes, but there was evidence of several recombination events in SSL-2009a. The results of the comparative genomic analyses further the understanding of the evolution and relationship between EP23 and its bacteriophage relatives.
-
Citations
Citations to this article as recorded by- Bacteriophage Treatment Induces Phenotype Switching and Alters Antibiotic Resistance of ESBL Escherichia coli
Árpád Rózsa, László Orosz, Nikoletta Szemerédi, Gabriella Spengler, Gábor Kecskeméti, Otília Vágó, Károly Péter Sárvári, Diana Szabó, Zoltán Szabó, Katalin Burián, Dezső Péter Virok
Antibiotics.2025; 14(1): 76. CrossRef - Characterization and application of novel bacteriophage PS2 for controlling pathogenic Escherichia coli in different food matrices
Song Zhang, Kanghee Ryu, Jin-Chul Kim, Juhee Ahn
International Journal of Food Microbiology.2025; 441: 111330. CrossRef - A novel lytic bacteriophage against colistin-resistant Escherichia coli isolated from different animals
Tianshi Xiao, Xiaolin Zhu, Wenhui Wang, Xuchen Jia, Changmei Guo, Xue Wang, Zhihui Hao
Virus Research.2023; 329: 199090. CrossRef - Comparative Genomics of a Polyvalent Escherichia-Salmonella Phage fp01 and In Silico Analysis of Its Receptor Binding Protein and Conserved Enterobacteriaceae Phage Receptor
Ignacio Vasquez, Julio Retamales, Barbara Parra, Vimbai Machimbirike, James Robeson, Javier Santander
Viruses.2023; 15(2): 379. CrossRef -
Complete Genome Sequences of Five SO-1-Like
Siphoviridae
Bacteriophages That Infect
Enterobacteriales
Madelyn G. Purnell, Kyle Andersen, Adam Bell, Jared T. Briscoe, Hannah M. F. Brown, Emilee L. Carr, Justen Doney, Parker F. Folsom, Cheyanne Green, Evan H. Harris, Elisa Huhem, R. Matthew Jensen, Liberty Johnson, Carter Jones, Andrew S. Lambert, Emily Loe
Microbiology Resource Announcements.2022;[Epub] CrossRef - Molecular Characteristics of Novel Phage vB_ShiP-A7 Infecting Multidrug-Resistant Shigella flexneri and Escherichia coli, and Its Bactericidal Effect in vitro and in vivo
Jing Xu, Ruiyang Zhang, Xinyan Yu, Xuesen Zhang, Genyan Liu, Xiaoqiu Liu
Frontiers in Microbiology.2021;[Epub] CrossRef - Virome in Fecal Samples From Wild Giant Pandas (Ailuropoda Melanoleuca)
Songyi Ning, Xiang Lu, Min Zhao, Xiaochun Wang, Shixing Yang, Quan Shen, Hao Wang, Wen Zhang
Frontiers in Veterinary Science.2021;[Epub] CrossRef - Isolation, characterization, and genomic analysis of the novel T4-like bacteriophage ΦCJ20
Jaegon Kim, Jong Pyo Chae, Gyeong-Hwuii Kim, Jae-Won Kim, Na-Gyeong Lee, Jun-Ok Moon, Sung-Sik Yoon
Food Science and Biotechnology.2021; 30(5): 735. CrossRef - Ecology, Structure, and Evolution of Shigella Phages
Sundharraman Subramanian, Kristin N. Parent, Sarah M. Doore
Annual Review of Virology.2020; 7(1): 121. CrossRef - Genomic and Proteomic Characterizations of Sfin-1, a Novel Lytic Phage Infecting Multidrug-Resistant Shigella spp. and Escherichia coli C
SK Tousif Ahamed, Banibrata Roy, Utpal Basu, Shanta Dutta, A. N. Ghosh, Boudhayan Bandyopadhyay, Nabanita Giri
Frontiers in Microbiology.2019;[Epub] CrossRef - Still Something to Discover: Novel Insights into Escherichia coli Phage Diversity and Taxonomy
Imke H. E. Korf, Jan P. Meier-Kolthoff, Evelien M. Adriaenssens, Andrew M. Kropinski, Manfred Nimtz, Manfred Rohde, Mark J. van Raaij, Johannes Wittmann
Viruses.2019; 11(5): 454. CrossRef - Comparative analysis and characterization of Enterobacteria phage SSL-2009a and ‘HK578likevirus’ bacteriophages
Shu Li, Shuguang Lu, Hui Huang, Li Tan, Qingshan Ni, Weilong Shang, Yi Yang, Zhen Hu, Junmin Zhu, Ming Li, Xiancai Rao, Fuquan Hu, Xiaomei Hu
Virus Research.2019; 259: 77. CrossRef - Characterization and Genomic Study of Phage vB_EcoS-B2 Infecting Multidrug-Resistant Escherichia coli
Yue Xu, Xinyan Yu, Yu Gu, Xu Huang, Genyan Liu, Xiaoqiu Liu
Frontiers in Microbiology.2018;[Epub] CrossRef - Three New Escherichia coli Phages from the Human Gut Show Promising Potential for Phage Therapy
Marion Dalmasso, Ronan Strain, Horst Neve, Charles M. A. P. Franz, Fabien J. Cousin, R. Paul Ross, Colin Hill, Raymond Schuch
PLOS ONE.2016; 11(6): e0156773. CrossRef - HostPhinder: A Phage Host Prediction Tool
Julia Villarroel, Kortine Kleinheinz, Vanessa Jurtz, Henrike Zschach, Ole Lund, Morten Nielsen, Mette Larsen
Viruses.2016; 8(5): 116. CrossRef - Life-Style and Genome Structure of Marine Pseudoalteromonas Siphovirus B8b Isolated from the Northwestern Mediterranean Sea
Elena Lara, Karin Holmfeldt, Natalie Solonenko, Elisabet Laia Sà, J. Cesar Ignacio-Espinoza, Francisco M. Cornejo-Castillo, Nathan C. Verberkmoes, Dolors Vaqué, Matthew B. Sullivan, Silvia G. Acinas, Christina A. Kellogg
PLOS ONE.2015; 10(1): e0114829. CrossRef - Integration of genomic and proteomic analyses in the classification of the Siphoviridae family
Evelien M. Adriaenssens, Rob Edwards, John H.E. Nash, Padmanabhan Mahadevan, Donald Seto, Hans-Wolfgang Ackermann, Rob Lavigne, Andrew M. Kropinski
Virology.2015; 477: 144. CrossRef - Isolation and development of bioluminescent reporter phages for bacterial dysentery
D. A. Schofield, D. J. Wray, I. J. Molineux
European Journal of Clinical Microbiology & Infectious Diseases.2015; 34(2): 395. CrossRef - Coverage of diarrhoea‐associated Escherichia coli isolates from different origins with two types of phage cocktails
Gilles Bourdin, Armando Navarro, Shafiqul A. Sarker, Anne‐C. Pittet, Firdausi Qadri, Shamima Sultana, Alejandro Cravioto, Kaisar A. Talukder, Gloria Reuteler, Harald Brüssow
Microbial Biotechnology.2014; 7(2): 165. CrossRef - Characterization and complete genome sequence of the Shigella bacteriophage pSf-1
Jin Woo Jun, Ji Hyung Kim, Sang Phil Shin, Jee Eun Han, Ji Young Chai, Se Chang Park
Research in Microbiology.2013; 164(10): 979. CrossRef - Bacteriophages for managingShigellain various clinical and non-clinical settings
Lawrence D. Goodridge
Bacteriophage.2013; 3(1): e25098. CrossRef
- Bacteriophage Treatment Induces Phenotype Switching and Alters Antibiotic Resistance of ESBL Escherichia coli
First
Prev
TOP
