In this study, we sought to isolate Salmonella Enteritidis-specific
lytic bacteriophages (phages), and we found a lytic phage
that could lyse not only S. Enteritidis but also other Gramnegative
foodborne pathogens. This lytic phage, SS3e, could
lyse almost all tested Salmonella enterica serovars as well as
other enteric pathogenic bacteria including Escherichia coli,
Shigella sonnei, Enterobacter cloacae, and Serratia marcescens.
This SS3e phage has an icosahedral head and a long tail, indicating
belong to the Siphoviridae. The genome was 40,793
base pairs, containing 58 theoretically determined open reading
frames (ORFs). Among the 58 ORFs, ORF49, and ORF25
showed high sequence similarity with tail spike protein and
lysozyme-like protein of Salmonella phage SE2, respectively,
which are critical proteins recognizing and lysing host bacteria.
Unlike SE2 phage whose host restricted to Salmonella
enterica serovars Enteritidis and Gallinarum, SS3e showed
broader host specificity against Gram-negative enteric bacteria;
thus, it could be a promising candidate for the phage
utilization against various Gram-negative bacterial infection
including foodborne pathogens.
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