Journal of Microbiology

Journal Articles

Tubulysin Production by the Dead Cells of Archangium gephyra KYC5002: Seohui Park, Chaehyeon Park, Yujin Ka, Kyungyun Cho; J. Microbiol. 2024;62(6):463-471. Published online June 13, 2024; DOI: https://doi.org/10.1007/s12275-024-00130-3

62 View
0 Download

Abstract: Archangium gephyra KYC5002 produces tubulysins during the death phase. In this study, we aimed to determine whether dead cells produce tubulysins. Cells were cultured for three days until the verge of the death phase, disrupted via ultrasonication, incubated for 2 h, and examined for tubulysin production. Non-disrupted cells produced 0.14 mg/L of tubulysin A and 0.11 mg/L of tubulysin B. Notably, tubulysin A production was increased by 4.4-fold to 0.62 mg/L and that of tubulysin B was increased by 6.7-fold to 0.74 mg/L in the disrupted cells. The same increase in tubulysin production was observed when the cells were killed by adding hydrogen peroxide. However, when the enzymes were inactivated via heat treatment of the cultures at 65 °C for 30 min, no significant increase in tubulysin production due to cell death was observed. Reverse transcription-quantitative polymerase chain reaction analysis of tubB mRNA revealed that the expression levels of tubulysin biosynthetic enzyme genes increased during the death phase compared to those during the vegetative growth phase. Our findings suggest that A. gephyra produces biosynthetic enzymes and subsequently uses them for tubulysin production in the cell death phase or during cell lysis by predators.

In Silico Intensive Analysis for the E4 Gene Evolution of Human Adenovirus Species D: Chanhee Lee, Anyeseu Park, Jeong Yoon Lee; J. Microbiol. 2024;62(5):409-418. Published online April 30, 2024; DOI: https://doi.org/10.1007/s12275-024-00132-1

59 View
0 Download

Abstract: Adenovirus (Ad) is a ubiquitous pathogen capable of infecting a wide range of animals and humans. Human Adenovirus (HAdV) can cause severe infection, particularly in individuals with compromised immune systems. To date, over 110 types of HAdV have been classified into seven species from A to G, with the majority belonging to the human adenovirus species D (HAdV-D). In the HAdV-D, the most significant factor for the creation of new adenovirus types is homologous recombination between viral genes involved in determining the virus tropism or evading immune system of host cells. The E4 gene, consisting of seven Open Reading Frames (ORFs), plays a role in both the regulation of host cell metabolism and the replication of viral genes. Despite long-term studies, the function of each ORF remains unclear. Based on our updated information, ORF2, ORF3, and ORF4 have been identified as regions with relatively high mutations compared to other ORFs in the E4 gene, through the use of in silico comparative analysis. Additionally, we managed to visualize high mutation sections, previously undetectable at the DNA level, through a powerful amino acid sequence analysis tool known as proteotyping. Our research has revealed the involvement of the E4 gene in the evolution of human adenovirus, and has established accurate sequence information of the E4 gene, laying the groundwork for further research.

Transcriptome‑based Mining of the Constitutive Promoters for Tuning Gene Expression in Aspergillus oryzae: Kobkul Laoteng , Jutamas Anantayanon , Chanikul Chutrakul , Sarocha Panchanawaporn , Sukanya Jeennor; J. Microbiol. 2023;61(2):199-210. Published online February 6, 2023; DOI: https://doi.org/10.1007/s12275-023-00020-0

59 View
0 Download
5 Web of Science
6 Crossref

Abstract

Transcriptional regulation has been adopted for developing metabolic engineering tools. The regulatory promoter is a crucial genetic element for strain optimization. In this study, a gene set of Aspergillus oryzae with highly constitutive expression across different growth stages was identified through transcriptome data analysis. The candidate promoters were functionally characterized in A. oryzae by transcriptional control of β-glucuronidase (GUS) as a reporter. The results showed that the glyceraldehyde triphosphate dehydrogenase promoter (PgpdA1) of A. oryzae with a unique structure displayed the most robust strength in constitutively controlling the expression compared to the PgpdA2 and other putative promoters tested. In addition, the ubiquitin promoter (Pubi) of A. oryzae exhibited a moderate expression strength. The deletion analysis revealed that the 5' untranslated regions of gpdA1 and ubi with the length of 1028 and 811 nucleotides, counted from the putative translation start site (ATG), respectively, could efficiently drive the GUS expression. Interestingly, both promoters could function on various carbon sources for cell growth. Glucose was the best fermentable carbon source for allocating high constitutive expressions during cell growth, and the high concentrations (6–8% glucose, w/v) did not repress their functions. It was also demonstrated that the secondary metabolite gene coding for indigoidine could express under the control of PgpdA1 or Pubi promoter. These strong and moderate promoters of A. oryzae provided beneficial options in tuning the transcriptional expression for leveraging the metabolic control towards the targeted products.

Citations

Citations to this article as recorded by

Construction of an Aspergillus oryzae △nptB△pyrG Host for Homologous Expression of Lipase and Catalytic Property Characterization of Recombinant Lipase
Yueting Zhang, Hongmei Nie, Fei Zhang, Mengmeng Jin, Zhao Wang, Jianyong Zheng
Applied Biochemistry and Biotechnology.2025; 197(2): 873. CrossRef
Transcriptome-Based Mining of the Strong Promoters for Hyperproduction of Gibberellin GA3 in Fusarium fujikuroi
Qi Guo, Yue-Feng Zhong, Xin-Yu Chen, Ya-Wen Li, Yu-Xin Yang, Zhi-Kui Nie, Tian-Qiong Shi
Journal of Agricultural and Food Chemistry.2025; 73(14): 8440. CrossRef
Mining and Understanding of New Transcriptional Regulatory Elements from Licorice-Derived Endophyte Serratia Rubidaea W12-1
Ying Zhang, Yunyang Ma, Bing Hu, H.M. Zabed, A.K. Singh, M.A. Ibrahim, N. Chen
BIO Web of Conferences.2024; 142: 03018. CrossRef
Development of Aspergillus oryzae BCC7051 as a Robust Cell Factory Towards the Transcriptional Regulation of Protease-Encoding Genes for Industrial Applications
Sarocha Panchanawaporn, Chanikul Chutrakul, Sukanya Jeennor, Jutamas Anantayanon, Kobkul Laoteng
Journal of Fungi.2024; 11(1): 6. CrossRef
Exploring and Engineering Novel Strong Promoters for High-Level Protein Expression in Bacillus subtilis DB104 through Transcriptome Analysis
Ji-Su Jun, Hyang-Eun Jeong, Kwang-Won Hong
Microorganisms.2023; 11(12): 2929. CrossRef
Efficient de novo production of bioactive cordycepin by Aspergillus oryzae using a food-grade expression platform
Sukanya Jeennor, Jutamas Anantayanon, Sarocha Panchanawaporn, Chanikul Chutrakul, Wanwipa Vongsangnak, Kobkul Laoteng
Microbial Cell Factories.2023;[Epub] CrossRef

Flavihumibacter fluminis sp. nov. and Flavihumibacter rivuli sp. nov., isolated from a freshwater stream: Miri S. Park , Hyeonuk Sa , Ilnam Kang , Jang-Cheon Cho; J. Microbiol. 2022;60(8):806-813. Published online July 29, 2022; DOI: https://doi.org/10.1007/s12275-022-2298-2

66 View
0 Download
5 Web of Science
6 Crossref

Abstract

Two Gram-stain-positive, aerobic, chemoheterotrophic, nonmotile, rod-shaped, and yellow-pigmented bacterial strains, designated IMCC34837T and IMCC34838T, were isolated from a freshwater stream. Results of 16S rRNA gene-based phylogenetic analyses showed that strains IMCC34837T and IMCC- 34838T shared 96.3% sequence similarity and were most closely related to Flavihumibacter profundi Chu64-6-1T (99.6%) and Flavihumibacter cheonanensis WS16T (96.4%), respectively. Complete whole-genome sequences of strains IMCC- 34837T and IMCC34838T were 5.0 Mbp and 4.3 Mbp of genome size with 44.5% and 47.9% of DNA G + C contents, respectively. Average nucleotide identity (ANI) and digital DNA- DNA hybridization (dDDH) values between the two strains were 70.0% and 17.9%, repectively, revealing that they are independent species. The two strains showed ≤ 75.2% ANI and ≤ 19.3% dDDH values to each closely related species of the genus Flavihumibacter, indicating that the two strains represent each novel species. Major fatty acid constituents of strain IMCC34837T were iso-C15:0, iso-C15:1 G and anteiso-C15:0 and those of strain IMCC34838T were iso-C15:0 and iso-C15:1 G. The predominant isoprenoid quinone detected in both strains was menaquinone-7 (MK-7). Major polar lipids of both strains were phosphatidylethanolamine, aminolipids, and glycolipids. Based on the phylogenetic and phenotypic characterization, strains IMCC34837T and IMCC34838T were considered to represent two novel species within the genus Flavihumibacter, for which the names Flavihumibacter fluminis sp. nov. and Flavihumibacter rivuli sp. nov. are proposed with IMCC34837T (= KACC 21752T = NBRC 115292T) and IMCC34838T (= KACC 21753T = NBRC 115293T) as the type strains, respectively.

Citations

Citations to this article as recorded by

Update on the proposed minimal standards for the use of genome data for the taxonomy of prokaryotes
Raúl Riesco, Martha E. Trujillo
International Journal of Systematic and Evolutionary Microbiology .2024;[Epub] CrossRef
Leuconostoc aquikimchii sp. nov., a Lactic Acid Bacterium Isolated from Cabbage Watery Kimchi
Subin Kim, Se Hee Lee, Ki Hyun Kim, Misun Yun
Journal of Microbiology.2024; 62(12): 1089. CrossRef
Congregibacter variabilis sp. nov. and Congregibacter brevis sp. nov. Within the OM60/NOR5 Clade, Isolated from Seawater, and Emended Description of the Genus Congregibacter
Hyeonsu Tak, Miri S. Park, Hyerim Cho, Yeonjung Lim, Jang-Cheon Cho
Journal of Microbiology.2024; 62(9): 739. CrossRef
Flavobacterium rivulicola sp. nov., Isolated from a Freshwater Stream
Sumin Kim, Miri S. Park, Ilnam Kang, Jang-Cheon Cho
Current Microbiology.2024;[Epub] CrossRef
Validation List no. 211. Valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, Markus Göker
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef
Proposal of Flavihumibacter fluvii sp. nov. as a replacement name for the effectively published but invalidated epithet Flavihumibacter fluminis Park et al. 2022
Miri S. Park, Hyeonuk Sa, Ilnam Kang, Jang-Cheon Cho
Journal of Microbiology.2023; 61(6): 649. CrossRef

Regulatory role of cysteines in (2R, 3R)-butanediol dehydrogenase BdhA of Bacillus velezensis strain GH1-13: Yunhee Choi , Yong-Hak Kim; J. Microbiol. 2022;60(4):411-418. Published online March 14, 2022; DOI: https://doi.org/10.1007/s12275-022-2018-y

61 View
0 Download
3 Web of Science
3 Crossref

Abstract

Bacillus velezensis strain GH1-13 contains a (2R,3R)-butanediol dehydrogenase (R-BDH) BdhA which converts acetoin to R-BD reversibly, however, little is known about its regulatory cysteine and biological significance. We performed sitedirected mutation of three cysteines in BdhA. The C37S mutant had no enzyme activity and the C34S and C177S mutants differed from each other and wild type (WT). After zinc affinity chromatography, 1 mM ZnCl2 treatment resulted in a 3-fold enhancement of the WT activity, but reduced activity of the C34S mutant by more than 2 folds compared to the untreated ones. However, ZnCl2 treatment did not affect the activity of the C177S mutant. Most of the double and triple mutant proteins (C34S/C37S, C34S/C177S, C37S/C177S, and C34S/C37S/C177S) were aggregated in zinc resins, likely due to the decreased protein stability. All of the purified WT and single mutant proteins increased multiple intermolecular disulfide bonds in the presence of H2O2 as the buffer pH decreased from 7.5 to 5.5, whereas an intramolecular disulfide bond of cysteine 177 and another cysteine in the CGIC motif region was likely formed at pH higher than pKa of 7.5. When pH varied, WT and its C34S or C177S mutants reduced acetoin to R-BD at the optimum pH 5.5 and oxidized R-BD to acetoin at the optimum pH 10. This study demonstrated that cysteine residues in BdhA play a regulatory role for the production of acetoin and R-BD depending on pH as well as metal binding and oxidative stress.

Citations

Citations to this article as recorded by

Significantly enhanced specific activity of Bacillus subtilis (2,3)-butanediol dehydrogenase through computer-aided refinement of its substrate-binding pocket
Bochun Hu, Xiaoqi Xi, Fugang Xiao, Xiaomeng Bai, Yuanyuan Gong, Yifan Li, Xueqin Qiao, Cunduo Tang, Jihong Huang
International Journal of Biological Macromolecules.2024; 281: 136443. CrossRef
Structural and enzymatic characterization of Bacillus subtilis R,R-2,3-butanediol dehydrogenase
Xiaofei Wang, Lingyun Jia, Fangling Ji
Biochimica et Biophysica Acta (BBA) - General Subjects.2023; 1867(4): 130326. CrossRef
Engineering a BsBDHA substrate-binding pocket entrance for the improvement in catalytic performance toward (R)-phenyl-1,2-ethanediol based on the computer-aided design
Bo-Chun Hu, Meng-Ran Li, Ying-Ying Li, Xin-Shuang Yuan, Yu-Ye Hu, Fu-Gang Xiao
Biochemical Engineering Journal.2023; 194: 108907. CrossRef

Activity of Lactobacillus crispatus isolated from vaginal microbiota against Mycobacterium tuberculosis: Youngkyoung Lee , Hoonhee Seo , Sukyung Kim Abdur Rahim , Youjin Yoon , Jehee Jung , Saebim Lee , Chang Beom Ryu , Ho-Yeon Song; J. Microbiol. 2021;59(11):1019-1030. Published online November 1, 2021; DOI: https://doi.org/10.1007/s12275-021-1332-0

61 View
0 Download
7 Web of Science
7 Crossref

Abstract

Tuberculosis, an infectious disease, is caused by Mycobacterium tuberculosis. It remains a significant public health issue around the globe, causing about 1.8 million deaths every year. Drug-resistant M. tuberculosis, including multi-drug-resistant (MDR), extremely-drug-resistant (XDR), and totally drugresistant (TDR) M. tuberculosis, continues to be a threat to public health. In the case of antibiotic-resistant tuberculosis, the treatment effect of conventional antibiotics is low. Side effects caused by high doses over a long period are causing severe problems. To overcome these problems, there is an urgent need to develop a new anti-tuberculosis drug that is different from the existing compound-based antibiotics. Probiotics are defined as live microorganisms conferring health benefits. They can be potential therapeutic agents in this context as the effectiveness of probiotics against different infectious diseases has been well established. Here, we report that Lactobacillus crispatus PMC201 shows a promising effect on tuberculosis isolated from vaginal fluids of healthy Korean women. Lactobacillus crispatus PMC201 reduced M. tuberculosis H37Rv under co-culture conditions in broth and reduced M. tuberculosis H37Rv and XDR M. tuberculosis in macrophages. Lactobacillus crispatus PMC201 was not toxic to a guinea pig model and did not induce dysbiosis in a human intestinal microbial ecosystem simulator. Taken together, these
results
indicate that L. crispatus PMC201 can be a promising alternative drug candidate in the current tuberculosis drug regime. Further study is warranted to assess the in vivo efficacy and confirm the mode of action of L. crispatus PMC201.

Citations

Citations to this article as recorded by

Exploring the potential of Lactocaseibacillus rhamnosus PMC203 in inducing autophagy to reduce the burden of Mycobacterium tuberculosis
Md Abdur Rahim, Hoonhee Seo, Sukyung Kim, Indrajeet Barman, Fatemeh Ghorbanian, Mohammed Solayman Hossain, Md Sarower Hossen Shuvo, Saebim Lee, Ho-Yeon Song
Medical Microbiology and Immunology.2024;[Epub] CrossRef
Efficacy of lyophilized Lactobacillus sakei as a potential candidate for preventing carbapenem-resistant Klebsiella infection
Hanieh Tajdozian, Hoonhee Seo, Yoonkyoung Jeong, Fatemeh Ghorbanian, Chae-eun Park, Faezeh Sarafraz, Md Abdur Rahim, Youngkyoung Lee, Sukyung Kim, Saebim Lee, Jung-Hyun Ju, Chul-Ho Kim, Ho-Yeon Song
Annals of Microbiology.2024;[Epub] CrossRef
Identification of Probiotic Strains with Anti-Tuberculosis Activity and Their Characterization as Potential Therapeutic Agents
Mohammed Solayman Hossain, Hoonhee Seo, Md Abdur Rahim, Md Sarower Hossen Shuvo, Indrajeet Barman, Hokyoung Kim, Jinhyeon An, Sukyung Kim, Ho-Yeon Song
Journal of Bacteriology and Virology.2024; 54(4): 325. CrossRef
The gut and lung microbiota in pulmonary tuberculosis: susceptibility, function, and new insights into treatment
Qiqi Zhuo, Xianyi Zhang, Kehong Zhang, Chan Chen, Zhen Huang, Yuzhong Xu
Expert Review of Anti-infective Therapy.2023; 21(12): 1355. CrossRef
Host microbiome in tuberculosis: disease, treatment, and immunity perspectives
Archana Pant, Bhabatosh Das, Gopalakrishnan Aneeshkumar Arimbasseri
Frontiers in Microbiology.2023;[Epub] CrossRef
Antibiotic Resistance to Mycobacterium tuberculosis and Potential Use of Natural and Biological Products as Alternative Anti-Mycobacterial Agents
Roberto Arrigoni, Andrea Ballini, Skender Topi, Lucrezia Bottalico, Emilio Jirillo, Luigi Santacroce
Antibiotics.2022; 11(10): 1431. CrossRef
In Vivo Efficacy of Bacillus velezensis Isolated from Korean Gochang Bokbunja Vinegar against Carbapenem-Resistant Klebsiella pneumoniae Infections
Fatemeh Ghorbanian, Hoonhee Seo, Hanieh Tajdozian, Youngkyoung Lee, MD Abdur Rahim, Sukyung Kim, Il-Yun Jung, Saebim Lee, Ho-Yeon Song
Polish Journal of Microbiology.2022; 71(4): 553. CrossRef

Salmonella Typhimurium ST313 isolated in Brazil revealed to be more invasive and inflammatory in murine colon compared to ST19 strains: Amanda Aparecida Seribelli , Tamara R. Machado Ribeiro , Patrick da Silva† , Isabela Mancini Martins , Felipe Pinheiro Vilela , Marta I. Cazentini Medeiros , Kamila Chagas Peronni , Wilson Araújo da Silva Junior , Cristiano Gallina Moreira , Juliana Pfrimer Falcão; J. Microbiol. 2021;59(9):861-870. Published online August 12, 2021; DOI: https://doi.org/10.1007/s12275-021-1082-z

61 View
0 Download
6 Web of Science
6 Crossref

Abstract

Salmonella Typhimurium (ST313) has caused an epidemic of invasive disease in sub-Saharan Africa and has been recently identified in Brazil. As the virulence of this ST is poorly understood, the present study aimed to (i) perform the RNAseq in vitro of S. Typhimurium STm30 (ST313) grown in Luria-Bertani medium at 37°C; (ii) compare it with the RNAseq of the S. Typhimurium SL1344 (ST19) and S. Typhimurium STm11 (ST19) strains under the same growing conditions; and (iii) examine the colonization capacity and expression of virulence genes and cytokines in murine colon. The STm30 (ST313) strain exhibited stronger virulence and was associated with a more inflammatory profile than the strains SL1344 (ST19) and STm11 (ST19), as demonstrated by transcriptome and in vivo assay. The expression levels of the hilA, sopD2, pipB, and ssaS virulence genes, other Salmonella pathogenicity islands SPI-1 and SPI-2 genes or effectors, and genes of the cytokines IL-1β, IFN-γ, TNF-α, IL-6, IL-17, IL-22, and IL-12 were increased during ST313 infection in C57BL/6J mice. In conclusion, S. Typhimurium STm30 (ST313) isolated from human feces in Brazil express higher levels of pathogenesis- related genes at 37°C and has stronger colonization and invasion capacity in murine colon due to its high expression levels of virulence genes, when compared with the S. Typhimurium SL1344 (ST19) and STm11 (ST19) strains. STm30 (ST313) also induces stronger expression of pro-inflammatory cytokines in this organ, suggesting that it causes more extensive tissue damage.

Citations

Citations to this article as recorded by

Whole-Genome Sequencing Reveals the Population Structure and Genetic Diversity of Salmonella Typhimurium ST34 and ST19 Lineages
Zhen-xu Zhuo, Yu-lian Feng, Xi-wei Zhang, Hao Liu, Fang-yin Zeng, Xiao-yan Li
Journal of Microbiology.2024; 62(10): 859. CrossRef
Incremental increases in physiological fluid shear progressively alter pathogenic phenotypes and gene expression in multidrug resistant Salmonella
Jiseon Yang, Jennifer Barrila, Eric A. Nauman, Seth D. Nydam, Shanshan Yang, Jin Park, Ami D. Gutierrez-Jensen, Christian L. Castro, C. Mark Ott, Kristina Buss, Jason Steel, Anne D. Zakrajsek, Mary M. Schuff, Cheryl A. Nickerson
Gut Microbes.2024;[Epub] CrossRef
Virulence potential of Salmonella 1,4, [5],12:i:- strains isolated during decades from different sources in the Southeast region of Brazil
Giovana do Nascimento Pereira, Amanda Aparecida Seribelli, Carolina Nogueira Gomes, Felipe Pinheiro Vilela, Ludmilla Tonani, Monique Ribeiro Tiba-Casas, Marta Inês Cazentini Medeiros, Dália dos Prazeres Rodrigues, Márcia Regina von Zeska Kress, Juliana Pf
Brazilian Journal of Microbiology.2023; 54(4): 2827. CrossRef
Invasive non-typhoidal Salmonella (iNTS) aminoglycoside-resistant ST313 isolates feature unique pathogenic mechanisms to reach the bloodstream
Isabela Mancini Martins, Amanda Aparecida Seribelli, Tamara R. Machado Ribeiro, Patrick da Silva, Bruna Cardinali Lustri, Rodrigo T. Hernandes, Juliana Pfrimer Falcão, Cristiano Gallina Moreira
Infection, Genetics and Evolution.2023; 116: 105519. CrossRef
Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Microbial Pathogenesis.2022; 165: 105460. CrossRef
Antimicrobial resistance and genetic background of non-typhoidal Salmonella enterica strains isolated from human infections in São Paulo, Brazil (2000–2019)
Aline Parolin Calarga, Marco Tulio Pardini Gontijo, Luiz Gonzaga Paula de Almeida, Ana Tereza Ribeiro de Vasconcelos, Leandro Costa Nascimento, Taíse Marongio Cotrim de Moraes Barbosa, Thalita Mara de Carvalho Perri, Silvia Regina dos Santos, Monique Ribe
Brazilian Journal of Microbiology.2022; 53(3): 1249. CrossRef

Zinc-binding domain mediates pleiotropic functions of Yvh1 in Cryptococcus neoformans: Jae-Hyung Jin , Myung Kyung Choi , Hyun-Soo Cho , Yong-Sun Bahn; J. Microbiol. 2021;59(7):658-665. Published online July 1, 2021; DOI: https://doi.org/10.1007/s12275-021-1287-1

45 View
0 Download

Abstract: Yvh1 is a dual-specificity phosphatase (DUSP) that is evolutionarily conserved in eukaryotes, including yeasts and humans. Yvh1 is involved in the vegetative growth, differentiation, and virulence of animal and plant fungal pathogens. All Yvh1 orthologs have a conserved DUSP catalytic domain at the N-terminus and a zinc-binding (ZB) domain with two zinc fingers (ZFs) at the C-terminus. Although the DUSP domain is implicated in the regulation of MAPK signaling in humans, only the ZB domain is essential for most cellular functions of Yvh1 in fungi. This study aimed to analyze the functions of the DUSP and ZB domains of Yvh1 in the human fungal pathogen Cryptococcus neoformans, whose Yvh1 (CnYvh1) contains a DUSP domain at the C-terminus and a ZB domain at the N-terminus. Notably, CnYvh1 has an extended internal domain between the two ZF motifs in the ZB domain. To elucidate the function of each domain, we constructed individual domain deletions and swapping strains by complementing the yvh1Δ mutant with wild-type (WT) or mutated YVH1 alleles and examined their Yvh1-dependent phenotypes, including growth under varying stress conditions, mating, and virulence factor production. Here, we found that the complementation of the yvh1Δ mutant with the mutated YVH1 alleles having two ZFs of the ZB domain, but not the DUSP and extended internal domains, restored the WT phenotypic traits in the yvh1Δ mutant. In conclusion, the ZB domain, but not the N-terminal DUSP domain, plays a pivotal role in the pathobiological functions of cryptococcal Yvh1.

Deep convolutional neural network: a novel approach for the detection of Aspergillus fungi via stereomicroscopy: Haozhong Ma , Jinshan Yang , Xiaolu Chen , Xinyu Jiang , Yimin Su , Shanlei Qiao , Guowei Zhong; J. Microbiol. 2021;59(6):563-572. Published online March 29, 2021; DOI: https://doi.org/10.1007/s12275-021-1013-z

62 View
0 Download
12 Web of Science
12 Crossref

Abstract

Fungi of the genus Aspergillus are ubiquitously distributed in nature, and some cause invasive aspergillosis (IA) infections in immunosuppressed individuals and contamination in agricultural products. Because microscopic observation and molecular detection of Aspergillus species represent the most operator-dependent and time-intensive activities, automated and cost-effective approaches are needed. To address this challenge, a deep convolutional neural network (CNN) was used to investigate the ability to classify various Aspergillus species. Using a dissecting microscopy (DM)/stereomicroscopy platform, colonies on plates were scanned with a 35× objective, generating images of sufficient resolution for classification. A total of 8,995 original colony images from seven Aspergillus species cultured in enrichment medium were gathered and autocut to generate 17,142 image crops as training and test datasets containing the typical representative morphology of conidiophores or colonies of each strain. Encouragingly, the Xception model exhibited a classification accuracy of 99.8% on the training image set. After training, our CNN model achieved a classification accuracy of 99.7% on the test image set. Based on the Xception performance during training and testing, this classification algorithm was further applied to recognize and validate a new set of raw images of these strains, showing a detection accuracy of 98.2%. Thus, our study demonstrated a novel concept for an artificial-intelligence-based and cost-effective detection
method
ology for Aspergillus organisms, which also has the potential to improve the public’s understanding of the fungal kingdom.

Citations

Citations to this article as recorded by

Outlier classification for microbiological open set recognition
Yining Pan, Wei Ye, Dejin Xie, Jiaoyu Wang, Hongkai Wang, Haiping Qiu
Computers and Electronics in Agriculture.2024; 224: 109104. CrossRef
Harnessing of Artificial Intelligence for the Diagnosis and Prevention of Hospital-Acquired Infections: A Systematic Review
Buket Baddal, Ferdiye Taner, Dilber Uzun Ozsahin
Diagnostics.2024; 14(5): 484. CrossRef
Current status and new experimental diagnostic methods of invasive fungal infections after hematopoietic stem cell transplantation
Zhenhua Tang, HaiTao Wang, Yuankai Liu, Chen Wang, Xinye Li, Qiong Yang
Archives of Microbiology.2024;[Epub] CrossRef
Artificial Intelligence: Exploring utility in detection and typing of fungus with futuristic application in fungal cytology
Nidhi Singla, Reetu Kundu, Pranab Dey
Cytopathology.2024; 35(2): 226. CrossRef
Label-Free Optical Transmission Tomography for Direct Mycological Examination and Monitoring of Intracellular Dynamics
Eliott Teston, Marc Sautour, Léa Boulnois, Nicolas Augey, Abdellah Dighab, Christophe Guillet, Dea Garcia-Hermoso, Fanny Lanternier, Marie-Elisabeth Bougnoux, Frédéric Dalle, Louise Basmaciyan, Mathieu Blot, Pierre-Emmanuel Charles, Jean-Pierre Quenot, Bi
Journal of Fungi.2024; 10(11): 741. CrossRef
Artificial Intelligence and Microbiology
Mert Kandilci, Gülfer Yakıcı, Mediha Begüm Kayar
Experimental and Applied Medical Science.2024; 5(2): 119. CrossRef
Attention-Guided Transfer Learning for Identification of Filamentous Fungi Encountered in the Clinical Laboratory
Tsi-Shu Huang, Kevin Wang, Xiu-Yuan Ye, Chii-Shiang Chen, Fu-Chuen Chang, Paschalis Vergidis, Yang Zhang
Microbiology Spectrum.2023;[Epub] CrossRef
Artificial Intelligence Based Test Systems to Resist Waterborne Diseases by Early and Rapid Identification of Pathogens: A Review
Chethna Joy, G. Naveen Sundar, D. Narmadha
SN Computer Science.2023;[Epub] CrossRef
Plant and Animal Species Recognition Based on Dynamic Vision Transformer Architecture
Hang Pan, Lun Xie, Zhiliang Wang
Remote Sensing.2022; 14(20): 5242. CrossRef
Machine Learning and Deep Learning Based Computational Approaches in Automatic Microorganisms Image Recognition: Methodologies, Challenges, and Developments
Priya Rani, Shallu Kotwal, Jatinder Manhas, Vinod Sharma, Sparsh Sharma
Archives of Computational Methods in Engineering.2022; 29(3): 1801. CrossRef
A Small Sample Recognition Model for Poisonous and Edible Mushrooms based on Graph Convolutional Neural Network
Li Zhu, Xin Pan, Xinpeng Wang, Fu Haito, Abdul Rehman Javed
Computational Intelligence and Neuroscience.2022; 2022: 1. CrossRef
Morphologic identification of clinically encountered moulds using a residual neural network
Ran Jing, Xiang-Long Yin, Xiu-Li Xie, He-Qing Lian, Jin Li, Ge Zhang, Wen-Hang Yang, Tian-Shu Sun, Ying-Chun Xu
Frontiers in Microbiology.2022;[Epub] CrossRef

Antarctic tundra soil metagenome as useful natural resources of cold-active lignocelluolytic enzymes: Han Na Oh , Doyoung Park , Hoon Je Seong , Dockyu Kim , Woo Jun Sul; J. Microbiol. 2019;57(10):865-873. Published online September 30, 2019; DOI: https://doi.org/10.1007/s12275-019-9217-1

52 View
0 Download
21 Web of Science
21 Crossref

Abstract

Lignocellulose composed of complex carbohydrates and aromatic heteropolymers is one of the principal materials for the production of renewable biofuels. Lignocellulose-degrading genes from cold-adapted bacteria have a potential to increase the productivity of biological treatment of lignocellulose biomass by providing a broad range of treatment temperatures. Antarctic soil metagenomes allow to access novel genes encoding for the cold-active lignocellulose-degrading enzymes, for biotechnological and industrial applications. Here, we investigated the metagenome targeting cold-adapted microbes in Antarctic organic matter-rich soil (KS 2-1) to mine lignolytic and celluloytic enzymes by performing single molecule, real-time metagenomic (SMRT) sequencing. In the assembled Antarctic metagenomic contigs with relative long reads, we found that 162 (1.42%) of total 11,436 genes were annotated as carbohydrate-active enzymes (CAZy). Actinobacteria, the dominant phylum in this soil’s metagenome, possessed most of candidates of lignocellulose catabolic genes like glycoside hydrolase families (GH13, GH26, and GH5) and auxiliary activity families (AA7 and AA3). The predicted lignocellulose degradation pathways in Antarctic soil metagenome showed synergistic role of various CAZyme harboring bacterial genera including Streptomyces, Streptosporangium, and Amycolatopsis. From phylogenetic relationships with cellular and environmental enzymes, several genes having potential for participating in overall lignocellulose degradation were also found. The results indicated the presence of lignocellulose-degrading bacteria in Antarctic tundra soil and the potential benefits of the lignocelluolytic enzymes as candidates for cold-active enzymes which will be used for the future biofuel-production industry.

Citations

Citations to this article as recorded by

Metagenomic insights into the lignocellulose degradation mechanism during short-term composting of peach sawdust: Core microbial community and carbohydrate-active enzyme profile analysis
Wei-Wei Zhang, Yu-Xin Guo, Qing-Jun Chen, Yi-Yang Wang, Qiu-Ying Wang, Ya-Ru Yang, Guo-Qing Zhang
Environmental Technology & Innovation.2025; 37: 103959. CrossRef
Comprehensive characterization and resistome analysis of Antarctic Pseudomonas migulae strain CAS19
Çiğdem Otur, Sezer Okay, Ömer Konuksever, Oğuzhan Duyar, Yılmaz Kaya, Aslıhan Kurt-Kızıldoğan
World Journal of Microbiology and Biotechnology.2024;[Epub] CrossRef
Response of carbohydrate-degrading enzymes and microorganisms to land use change in the southeastern Qinghai-Tibetan Plateau, China
Renhuan Zhu, Belayneh Azene, Piotr Gruba, Kaiwen Pan, Yalemzewd Nigussie, Awoke Guadie, Xiaoming Sun, Xiaogang Wu, Lin Zhang
Applied Soil Ecology.2024; 200: 105442. CrossRef
Investigating eukaryotic and prokaryotic diversity and functional potential in the cold and alkaline ikaite columns in Greenland
Mariane Schmidt Thøgersen, Athanasios Zervas, Peter Stougaard, Lea Ellegaard-Jensen
Frontiers in Microbiology.2024;[Epub] CrossRef
Bacterial Diversity, Metabolic Profiling, and Application Potential of Antarctic Soil Metagenomes
Mario Fernández, Salvador Barahona, Fernando Gutierrez, Jennifer Alcaíno, Víctor Cifuentes, Marcelo Baeza
Current Issues in Molecular Biology.2024; 46(11): 13165. CrossRef
Cold adaptation and response genes of Antarctic Cryobacterium sp. SO2 from the Fildes Peninsula, King George Island
Chui Peng Teoh, Marcelo González‑Aravena, Paris Lavin, Clemente Michael Vui Ling Wong
Polar Biology.2024; 47(2): 135. CrossRef
A bacterial cold-active dye-decolorizing peroxidase from an Antarctic Pseudomonas strain
Célica Cagide, Juan José Marizcurrena, Diego Vallés, Beatriz Alvarez, Susana Castro-Sowinski
Applied Microbiology and Biotechnology.2023; 107(5-6): 1707. CrossRef
Role of metagenomics in prospecting novel endoglucanases, accentuating functional metagenomics approach in second-generation biofuel production: a review
Ninian Prem Prashanth Pabbathi, Aditya Velidandi, Tanvi Tavarna, Shreyash Gupta, Ram Sarvesh Raj, Pradeep Kumar Gandam, Rama Raju Baadhe
Biomass Conversion and Biorefinery.2023; 13(2): 1371. CrossRef
Different Response of Plant- and Microbial-Derived Carbon Decomposition Potential between Alpine Steppes and Meadows on the Tibetan Plateau
Yanhong Yuan, Lan Chen, Jieying Wang, Yanfang Liu, Chengjie Ren, Yaoxin Guo, Jun Wang, Ninglian Wang, Fazhu Zhao, Wenying Wang
Forests.2023; 14(8): 1580. CrossRef
Investigation of cold-active and mesophilic cellulases: opportunities awaited
Sambhaji Chavan, Ashvini Shete, Yasmin Mirza, Mahesh S. Dharne
Biomass Conversion and Biorefinery.2023; 13(10): 8829. CrossRef
Cold-Adapted Proteases: An Efficient and Energy-Saving Biocatalyst
Zhengfeng Yang, Zhendi Huang, Qian Wu, Xianghua Tang, Zunxi Huang
International Journal of Molecular Sciences.2023; 24(10): 8532. CrossRef
Reclamation of abandoned saline-alkali soil increased soil microbial diversity and degradation potential
Fating Yin, Fenghua Zhang
Plant and Soil.2022; 477(1-2): 521. CrossRef
Recent advances in metagenomic analysis of different ecological niches for enhanced biodegradation of recalcitrant lignocellulosic biomass
Deepak Kukkar, Pushpender Kumar Sharma, Ki-Hyun Kim
Environmental Research.2022; 215: 114369. CrossRef
Novel bacterial taxa in a minimal lignocellulolytic consortium and their potential for lignin and plastics transformation
Carlos Andrés Díaz Rodríguez, Laura Díaz-García, Boyke Bunk, Cathrin Spröer, Katherine Herrera, Natalia A Tarazona, Luis M Rodriguez-R, Jörg Overmann, Diego Javier Jiménez
ISME Communications.2022;[Epub] CrossRef
The Use of Response Surface Methodology as a Statistical Tool for the Optimisation of Waste and Pure Canola Oil Biodegradation by Antarctic Soil Bacteria
Khadijah Nabilah Mohd Zahri, Azham Zulkharnain, Claudio Gomez-Fuentes, Suriana Sabri, Khalilah Abdul Khalil, Peter Convey, Siti Aqlima Ahmad
Life.2021; 11(5): 456. CrossRef
Comparison of Gut Microbiota between Gentoo and Adélie Penguins Breeding Sympatrically on Antarctic Ardley Island as Revealed by Fecal DNA Sequencing
Yin-Xin Zeng, Hui-Rong Li, Wei Han, Wei Luo
Diversity.2021; 13(10): 500. CrossRef
Deconstruction of Lignin: From Enzymes to Microorganisms
Jéssica P. Silva, Alonso R. P. Ticona, Pedro R. V. Hamann, Betania F. Quirino, Eliane F. Noronha
Molecules.2021; 26(8): 2299. CrossRef
Molecular Characterization of Novel Family IV and VIII Esterases from a Compost Metagenomic Library
Jong-Eun Park, Geum-Seok Jeong, Hyun-Woo Lee, Hoon Kim
Microorganisms.2021; 9(8): 1614. CrossRef
Illite/smectite clay regulating laccase encoded genes to boost lignin decomposition and humus formation in composting habitats revealed by metagenomics analysis
Qingran Meng, Susu Wang, Qiuqi Niu, Hailong Yan, Gen Li, Qiuhui Zhu, Qunliang Li
Bioresource Technology.2021; 338: 125546. CrossRef
Cultivation-independent and cultivation-dependent metagenomes reveal genetic and enzymatic potential of microbial community involved in the degradation of a complex microbial polymer
Ohana Y. A. Costa, Mattias de Hollander, Agata Pijl, Binbin Liu, Eiko E. Kuramae
Microbiome.2020;[Epub] CrossRef
Extremophile Microbial Communities and Enzymes for Bioenergetic Application Based on Multi-Omics Tools
Gislaine Fongaro, Guilherme Augusto Maia, Paula Rogovski, Rafael Dorighello Cadamuro, Joana Camila Lopes, Renato Simões Moreira, Aline Frumi Camargo, Thamarys Scapini, Fábio Spitza Stefanski, Charline Bonatto, Doris Sobral Marques Souza, Patrícia Hermes
Current Genomics.2020; 21(4): 240. CrossRef

Alcohol dehydrogenase 1 participates in the Crabtree effect and connects fermentative and oxidative metabolism in the Zygomycete Mucor circinelloides: Rosa Angélica Rangel-Porras , Sharel P. Díaz-Pérez , Juan Manuel Mendoza-Hernández , Pamela Romo-Rodríguez , Viridiana Alejandre-Castañeda , Marco I Valle-Maldonado , Juan Carlos Torres-Guzmán , Gloria Angélica González-Hernández , Jesús Campos-Garcia , José Arnau , Víctor Meza-Carmen , J. Félix Gutiérrez-Corona; J. Microbiol. 2019;57(7):606-617. Published online June 27, 2019; DOI: https://doi.org/10.1007/s12275-019-8680-z

55 View
0 Download
16 Web of Science
15 Crossref

Abstract

Mucor circinelloides is a dimorphic Zygomycete fungus that produces ethanol under aerobic conditions in the presence of glucose, which indicates that it is a Crabtree-positive fungus. To determine the physiological role of the alcohol dehydrogenase (ADH) activity elicited under these conditions, we obtained and characterized an allyl alcohol-resistant mutant that was defective in ADH activity, and examined the effect of adh mutation on physiological parameters related to carbon and energy metabolism. Compared to the Adh+ strain R7B, the ADH-defective (Adh-) strain M5 was unable to grow under anaerobic conditions, exhibited a considerable reduction in ethanol production in aerobic cultures when incubated with glucose, had markedly reduced growth capacity in the presence of oxygen when ethanol was the sole carbon source, and exhibited very low levels of NAD+-dependent alcohol dehydrogenase activity in the cytosolic fraction. Further characterization of the M5 strain showed that it contains a 10-bp deletion that interrupts the coding region of the adh1 gene. Complementation with the wild-type allele adh1+ by transformation of M5 remedied all the defects caused by the adh1 mutation. These findings indicate that in M. circinelloides, the product of the adh1 gene mediates the Crabtree effect, and can act as either a fermentative or an oxidative enzyme, depending on the nutritional conditions, thereby participating in the association between fermentative and oxidative metabolism. It was found that the spores of M. circinelloides possess low mRNA levels of the ethanol assimilation genes (adl2 and acs2), which could explain their inability to grow in the alcohol.

Citations

Citations to this article as recorded by

Fungal Alcohol Dehydrogenases: Physiological Function, Molecular Properties, Regulation of Their Production, and Biotechnological Potential
J. Félix Gutiérrez-Corona, Gloria Angélica González-Hernández, Israel Enrique Padilla-Guerrero, Vianey Olmedo-Monfil, Ana Lilia Martínez-Rocha, J. Alberto Patiño-Medina, Víctor Meza-Carmen, Juan Carlos Torres-Guzmán
Cells.2023; 12(18): 2239. CrossRef
Role of the nonribosomal peptide synthetase siderophore enzyme (Rfs) ofMucor lusitanicusin controlling the growth of fungal phytopathogens
Viridiana Alejandre-Castañeda, J. Alberto Patiño-Medina, Juan Bosco Guzmán-Pérez, Marco I. Valle-Maldonado, Javier Villegas, César R. Solorio-Alvarado, León Francisco Ruiz-Herrera, Rafael Ortiz-Alvarado, Karla Macías-Sánchez, Martha I. Ramírez-Díaz, Vícto
Canadian Journal of Microbiology.2023; 69(5): 185. CrossRef
Blood Serum Stimulates the Virulence Potential of Mucorales through Enhancement in Mitochondrial Oxidative Metabolism and Rhizoferrin Production
José Alberto Patiño-Medina, Viridiana Alejandre-Castañeda, Marco Iván Valle-Maldonado, Mauro Manuel Martínez-Pacheco, León Francisco Ruiz-Herrera, Joel Ramírez-Emiliano, Oscar Abelardo Ramírez-Marroquín, Karla Viridiana Castro-Cerritos, Jesús Campos-Garcí
Journal of Fungi.2023; 9(12): 1127. CrossRef
Effects of oxygen availability on mycobenthic communities of marine coastal sediments
Yanyan Yang, Carmen Alicia Rivera Pérez, Tim Richter-Heitmann, Rolf Nimzyk, Michael W. Friedrich, Marlis Reich
Scientific Reports.2023;[Epub] CrossRef
Transcription Factors Tec1 and Tec2 Play Key Roles in the Hyphal Growth and Virulence of Mucor lusitanicus Through Increased Mitochondrial Oxidative Metabolism
Viridiana Alejandre-Castañeda, J. Alberto Patiño-Medina, Marco I. Valle-Maldonado, Alexis García, Rafael Ortiz-Alvarado, León F. Ruíz-Herrera, Karla Viridiana Castro-Cerritos, Joel Ramírez-Emiliano, Martha I. Ramírez-Díaz, Victoriano Garre, Soo Chan Lee,
Journal of Microbiology.2023; 61(12): 1043. CrossRef
Secretion of the siderophore rhizoferrin is regulated by the cAMP-PKA pathway and is involved in the virulence of Mucor lusitanicus
Viridiana Alejandre-Castañeda, J. Alberto Patiño-Medina, Marco I. Valle-Maldonado, Rosa E. Nuñez-Anita, Gustavo Santoyo, Karla V. Castro-Cerritos, Rafael Ortiz-Alvarado, Alma R. Corrales-Escobosa, Martha I. Ramírez-Díaz, J. Felix Gutiérrez-Corona, Adolfo
Scientific Reports.2022;[Epub] CrossRef
Investigating the Effect of Alcohol Dehydrogenase Gene Knockout on Lipid Accumulation in Mucor circinelloides WJ11
Aabid Manzoor Shah, Hassan Mohamed, Abu Bakr Ahmad Fazili, Wu Yang, Yuanda Song
Journal of Fungi.2022; 8(9): 917. CrossRef
Beer production potentiality of some non-Saccharomyces yeast obtained from a traditional beer starter emao
Nitesh Boro, Ashis Borah, Rajib L. Sarma, Diganta Narzary
Brazilian Journal of Microbiology.2022; 53(3): 1515. CrossRef
Novel 2-aryl-4-aryloxyquinoline-based fungistatics for Mucor circinelloides. Biological evaluation of activity, QSAR and docking study
Pradip D. Nahide, Clara Alba-Betancourt, Rubén Chávez-Rivera, Pamela Romo-Rodríguez, Manuel Solís-Hernández, Luis A. Segura-Quezada, Karina R. Torres-Carbajal, Rocío Gámez-Montaño, Martha A. Deveze-Álvarez, Marco A. Ramírez-Morales, Angel J. Alonso-Castro
Bioorganic & Medicinal Chemistry Letters.2022; 63: 128649. CrossRef
Rapid detoxification of Jatropha curcas cake by fermentation with a combination of three microbial strains and characterization of their metabolic profiles
Zhenyu Zhang, Yaqi Chang, Min Wen, Hua Zhao, Xiaoling Chen, Gang Tian, Guangmang Liu, Jingyi Cai, Gang Jia
Journal of Applied Microbiology.2022; 133(2): 743. CrossRef
Oxic and Anoxic Organic Polymer Degradation Potential of Endophytic Fungi From the Marine Macroalga, Ecklonia radiata
Anita K. Perkins, Andrew L. Rose, Hans-Peter Grossart, Keilor Rojas-Jimenez, Selva K. Barroso Prescott, Joanne M. Oakes
Frontiers in Microbiology.2021;[Epub] CrossRef
Bio‐detoxification of Jatropha curcas L. cake by a soil‐borne Mucor circinelloides strain using a zebrafish survival model and solid‐state fermentation
Z. Zhang, Y. Chang, H. Tang, H. Zhao, X. Chen, G. Tian, G. Liu, J. Cai, G. Jia
Journal of Applied Microbiology.2021; 130(3): 852. CrossRef
Mass spore production of Mucor circinelloides on rice
J. Alberto Patiño-Medina, Viridiana Alejandre-Castañeda, Marco I. Valle-Maldonado, Javier Villegas, Martha I. Ramírez-Díaz, Rafael Ortiz-Alvarado, Víctor Meza-Carmen
3 Biotech.2021;[Epub] CrossRef
The heterotrimeric G‐protein beta subunit Gpb1 controls hyphal growth under low oxygen conditions through the protein kinase A pathway and is essential for virulence in the fungusMucor circinelloides
Marco Iván Valle‐Maldonado, José Alberto Patiño‐Medina, Carlos Pérez‐Arques, Nancy Yadira Reyes‐Mares, Irvin Eduardo Jácome‐Galarza, Rafael Ortíz‐Alvarado, Sandeep Vellanki, Martha Isela Ramírez‐Díaz, Soo Chan Lee, Victoriano Garre, Víctor Meza‐Carmen
Cellular Microbiology.2020;[Epub] CrossRef
Alteration of Fermentative Metabolism Enhances Mucor circinelloides Virulence
Sharel P. Díaz-Pérez, J. Alberto Patiño-Medina, Marco I. Valle-Maldonado, Adolfo López-Torres, Irvin E. Jácome-Galarza, Verónica Anaya-Martínez, Verónica Gómez-Ruiz, Jesús Campos-García, Rosa E. Nuñez-Anita, Rafael Ortiz-Alvarado, Martha I. Ramírez-Díaz,
Infection and Immunity.2020;[Epub] CrossRef

Oxygen-mediated growth enhancement of an obligate anaerobic archaeon Thermococcus onnurineus NA1: Seong Hyuk Lee , Hwan Youn , Sung Gyun Kang , Hyun Sook Lee; J. Microbiol. 2019;57(2):138-142. Published online January 31, 2019; DOI: https://doi.org/10.1007/s12275-019-8592-y

58 View
0 Download
3 Web of Science
2 Crossref

Abstract

Thermococcus onnurineus NA1, an obligate anaerobic hyperthermophilic archaeon, showed variable oxygen (O2) sensitivity depending on the types of substrate employed as an energy source. Unexpectedly, the culture with yeast extract as a sole energy source showed enhanced growth by 2-fold in the presence of O2. Genome-wide transcriptome analysis revealed the upregulation of several antioxidant-related genes encoding thioredoxin peroxidase (TON_0862), rubrerythrin (TON_0864), rubrerythrin-related protein (TON_0873), NAD(P)H rubredoxin oxidoreductase (TON_0865), or thioredoxin reductase (TON_1603), which can couple the detoxification of reactive oxygen species with the regeneration of NAD(P)+ from NAD(P)H. We present a plausible mechanism by which O2 serves to maintain the intracellular redox balance. This study demonstrates an unusual strategy of an obligate anaerobe underlying O2-mediated growth enhancement despite not having heme-based or cytochrome-type proteins.

Citations

Citations to this article as recorded by

How low can they go? Aerobic respiration by microorganisms under apparent anoxia
Jasmine S Berg, Soeren Ahmerkamp, Petra Pjevac, Bela Hausmann, Jana Milucka, Marcel M M Kuypers
FEMS Microbiology Reviews.2022;[Epub] CrossRef
Reductive evolution and unique predatory mode in the CPR bacterium Vampirococcus lugosii
David Moreira, Yvan Zivanovic, Ana I. López-Archilla, Miguel Iniesto, Purificación López-García
Nature Communications.2021;[Epub] CrossRef

Temporal and spatial impact of Spartina alterniflora invasion on methanogens community in Chongming Island, China: Xue Ping Chen , Jing Sun , Yi Wang , Heng Yang Zhang , Chi Quan He , Xiao Yan Liu , Nai Shun Bu , Xi-En Long; J. Microbiol. 2018;56(7):507-515. Published online June 14, 2018; DOI: https://doi.org/10.1007/s12275-018-8062-y

50 View
0 Download
10 Crossref

Abstract

Methane production by methanogens in wetland is recognized as a significant contributor to global warming. Spartina alterniflora (S. alterniflora), which is an invasion plant in China’s wetland, was reported to have enormous effects on methane production. But studies on shifts in the methanogen community in response to S. alterniflora invasion at temporal and spatial scales in the initial invasion years are rare. Sediments derived from the invasive species S. alterniflora and the native species Phragmites australis (P. australis) in pairwise sites and an invasion chronosequence patch (4 years) were analyzed to investigate the abundance and community structure of methanogens using quantitative real-time PCR (qPCR) and Denaturing gradient gel electrophoresis (DGGE) cloning of the methyl-coenzyme M reductase A (mcrA) gene. For the pairwise sites, the abundance of methanogens in S. alterniflora soils was lower than that of P. australis soils. For the chronosequence patch, the abundance and diversity of methanogens was highest in the soil subjected to two years invasion, in which we detected some rare groups including Methanocellales and Methanococcales. These results indicated a priming effect at the initial invasion stages of S. alterniflora for microorganisms in the soil, which was also supported by the diverse root exudates. The shifts of methanogen communities after S. alterniflora invasion were due to changes in pH, salinity and sulfate. The results indicate that root exudates from S. alterniflora have a priming effect on methanogens in the initial years after invasion, and the predominate methylotrophic groups (Methanosarcinales) may adapt to the availability of diverse substrates and reflects the potential for high methane production after invasion by S. alterniflora.

Citations

Citations to this article as recorded by

Stronger increase of methane emissions from coastal wetlands by non‐native Spartina alterniflora than non‐native Phragmites australis
Andrea Fuchs, Ian C. Davidson, J. Patrick Megonigal, John L. Devaney, Christina Simkanin, Genevieve L. Noyce, Meng Lu, Grace M. Cott
PLANTS, PEOPLE, PLANET.2025; 7(1): 62. CrossRef
Predictions of Spartina alterniflora leaf functional traits based on hyperspectral data and machine learning models
Wei Li, Xueyan Zuo, Zhijun Liu, Leichao Nie, Huazhe Li, Junjie Wang, Zhiguo Dou, Yang Cai, Xiajie Zhai, Lijuan Cui
European Journal of Remote Sensing.2024;[Epub] CrossRef
Salt marsh soil organic carbon is regulated by drivers of microbial activity
Hailey Erb, Ashley Keiser, Kristen M DeAngelis
Sustainable Microbiology.2024;[Epub] CrossRef
Asymmetric responses of functional microbes in methane and nitrous oxide emissions to plant invasion: A meta-analysis
Yanzhong Yao, Youtao Song, Pinjie Su, Jing Wang, Congke Miao, Yifu Luo, Qiqi Sun, Jiale Wang, Guohui Zhang, Naishun Bu, Zhaolei Li
Soil Biology and Biochemistry.2023; 178: 108931. CrossRef
Increasing coastal reclamation by Invasive alien plants and coastal armoring threatens the ecological sustainability of coastal wetlands
Jian Li, Zhanrui Leng, Taitiya Kenneth Yuguda, Lili Wei, Jiaojiao Xia, Chongyu Zhuo, Ziying Nie, Daolin Du
Frontiers in Marine Science.2023;[Epub] CrossRef
Spartina alterniflora Invaded Coastal Wetlands by Raising Soil Sulfur Contents: A Meta-Analysis
Zhenzhen Zhao, Liyu Cheng, Chiquan He, Feifei Wang, Jialin Liu, Yuanhang Li, Xueping Chen, Xiaoyan Liu, Gaoming Lv, Daoyuan Wang
Water.2022; 14(10): 1633. CrossRef
Exogenous nitrogen from riverine exports promotes soil methane production in saltmarshes in China
Chenhao Zhou, Yan Zhang, Songshuo Li, Qiuyue Jiang, Hongyang Chen, Ting Zhu, Xiao Xu, Hao Liu, Shiyun Qiu, Jihua Wu, Ming Nie, Bo Li
Science of The Total Environment.2022; 838: 156203. CrossRef
Exogenous Nitrogen from Riverine Exports Promotes Soil Methane Production in Saltmarshes in China
Chenhao Zhou, Yan Zhang, Songshuo Li, Qiuyue Jiang, Hongyang Chen, Ting Zhu, Xiao Xu, Hao Liu, Shi-Yun Qiu, Jihua Wu, Ming Nie, Bo Li
SSRN Electronic Journal .2021;[Epub] CrossRef
Spartina alterniflora raised soil sulfide content by regulating sulfur cycle-associated bacteria in the Jiuduansha Wetland of China
Chiquan He, Liyu Cheng, Daoyuan Wang, Zhenzhen Zhao, Zhengyu Wang, Feifei Wang, Xiaoxi Wang, Pu Zhang, Xueping Chen, Xiaoyan Liu
Plant and Soil.2021; 469(1-2): 107. CrossRef
The linkage between methane production activity and prokaryotic community structure in the soil within a shale gas field in China
Yan-Qin Wang, Guang-Quan Xiao, Yong-Yi Cheng, Ming-Xia Wang, Bo-Ya Sun, Zhi-Feng Zhou
Environmental Science and Pollution Research.2020; 27(7): 7453. CrossRef

Photosynthetic and biochemical responses of the freshwater green algae Closterium ehrenbergii Meneghini (Conjugatophyceae) exposed to the metal coppers and its implication for toxicity testing: Hui Wang , Vinitha Ebenezer , Jang-Seu Ki; J. Microbiol. 2018;56(6):426-434. Published online June 1, 2018; DOI: https://doi.org/10.1007/s12275-018-8081-8

53 View
0 Download
25 Crossref

Abstract

The freshwater green algae Closterium is sensitive to water quality, and hence has been suggested as ideal organisms for toxicity testing. In the present study, we evaluated the photosynthetic and biochemical responses of C. ehrenbergii to the common contaminants, coppers. The 72 h median effective concentrations (EC50) of CuSO4 and CuCl2 on the test organism were calculated to be 0.202 mg/L and 0.245 mg/L, respectively. Exposure to both coppers considerably decreased pigment levels and photosynthetic efficiency, while inducing the generation of reactive oxygen species (ROS) in cells with increased exposure time. Moreover, the coppers significantly increased the levels of lipid peroxidation and superoxide dismutase (SOD) activity, even at relatively lower concentrations. These suggest that copper contaminants may exert deleterious effects on the photosynthesis and cellular oxidative stress of C. ehrenbergii, representing its powerful potential in aquatic toxicity assessments.

Citations

Citations to this article as recorded by

Photosynthetic and antioxidant responses of Ankistrodesmus flexuosus and Curvastrum pantanale to environmentally relevant copper concentrations
Suleiman Dauda, Ana Teresa Lombardi
Physiologia Plantarum.2024;[Epub] CrossRef
Molecular characterization of a catalase gene in the freshwater green alga Closterium ehrenbergii and its putative function against abiotic stresses
Hui Wang, Peiling Wu, Fengru Li, Jeongmin Shin, Jang-Seu Ki
European Journal of Protistology.2024; 95: 126111. CrossRef
Two novel glutathione S-transferase (GST) genes in the toxic marine dinoflagellate Alexandrium pacificum and their transcriptional responses to environmental contaminants
Hyunjun Park, Han-Sol Kim, Sofia Abassi, Quynh Thi Nhu Bui, Jang-Seu Ki
Science of The Total Environment.2024; 915: 169983. CrossRef
Sub‐lethal effects of metals and pesticides on the freshwater dinoflagellate Palatinus apiculatus and environmental implications
Quynh Thi Nhu Bui, Taehee Kim, Han‐Sol Kim, Seokmin Lee, Seungjun Lee, Jang‐Seu Ki
Water Environment Research.2024;[Epub] CrossRef
Comparative assessment of algaecide performance on freshwater phytoplankton: Understanding differential sensitivities to frame cyanobacteria management
Malihe Mehdizadeh Allaf, Kevin J. Erratt, Hassan Peerhossaini
Water Research.2023; 234: 119811. CrossRef
Assessment of Membrane and Electrochemical Technologies for the Treatment of a Selenium-Bearing Mine water: Technology Performance and Effect on Toxicity and Bioaccumulation
Mohammadali Baghbanzadeh, Sylvain Dillon, Konstantin Volchek, Carrie Rickwood, Richard R. Goulet
Mine Water and the Environment.2023; 42(3): 361. CrossRef
Toxic effect of copper on soil microalgae: experimental data and critical review
Y. Maltsev, S. Maltseva, M. Kulikovskiy
International Journal of Environmental Science and Technology.2023; 20(10): 10903. CrossRef
Molecular cloning and oxidative‐stress responses of a novel Phi class glutathione S‐transferase (GSTF) gene in the freshwater algae Closterium ehrenbergii
Sofia Abassi, Hui Wang, Hansol Kim, Jang‐Seu Ki
Environmental Toxicology.2022; 37(4): 789. CrossRef
The effects of copper ions and copper nanomaterials on the output of amino acids from marine microalgae
Wenqiu Huang, Yuping Zhou, Ting Zhao, Liju Tan, Jiangtao Wang
Environmental Science and Pollution Research.2022; 29(7): 9780. CrossRef
Heavy metal–induced stress in eukaryotic algae—mechanisms of heavy metal toxicity and tolerance with particular emphasis on oxidative stress in exposed cells and the role of antioxidant response
Beatrycze Nowicka
Environmental Science and Pollution Research.2022; 29(12): 16860. CrossRef
Synthesis, characterisation and cytotoxicity of gold microwires for ultra-sensitive biosensor development
Nurul Akmal Che Lah, Robert Gray, Sonia Trigueros
Microbial Cell Factories.2021;[Epub] CrossRef
Transcriptomic sequencing reveals the response of Dunaliella salina to copper stress via the increased photosynthesis and carbon mechanism
Na Ling, Weilu Li, Guiguo Xu, Zheng Qi, Chenfeng Ji, Xiaorui Liu, Di Cui, Yuan Sun
Molecular Omics.2021; 17(5): 769. CrossRef
Evaluation of the operational conditions in the production and morphology of Chlorella sp.
L. Rendón-Castrillón, M. Ramírez-Carmona, C. Ocampo-López, R. Giraldo-Aristizabal
Brazilian Journal of Biology.2021; 81(1): 202. CrossRef
Self-polishing emulsion platforms: Eco-friendly surface engineering of coatings toward water borne marine antifouling
Jianbin Zhang, Yizhe Liu, Xingwei Wang, Chaoyang Zhang, Hui Liu, Wufang Yang, Meirong Cai, Xiaowei Pei, Feng Zhou
Progress in Organic Coatings.2020; 149: 105945. CrossRef
Molecular identification, differential expression and protective roles of iron/manganese superoxide dismutases in the green algae Closterium ehrenbergii against metal stress
Hui Wang, Jang-Seu Ki
European Journal of Protistology.2020; 74: 125689. CrossRef
Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress
Hui Wang, Jang‐Seu Ki
Environmental Toxicology.2020; 35(1): 5. CrossRef
Effect of polymeric biocide polyhexamethylene guanidine hydrochloride on morpho-physiological and biochemical parameters of wheat seedlings under copper stress
L. Lyoshyna, O. Tarasyuk, O. Bulko, S. Rogalsky, T. Kamenieva, M. Kuchuk
Agricultural Science and Practice.2020; 7(1): 49. CrossRef
The Growth, Population Size Structure, Physiological Changes, and Ultrastructure of the Microalga Picochlorum maculatum (Chlorophyta) under Sublethal Copper Concentrations
Zh. V. Markina
Russian Journal of Marine Biology.2020; 46(5): 346. CrossRef
Genome analyses provide insights into the evolution and adaptation of the eukaryotic Picophytoplankton Mychonastes homosphaera
Changqing Liu, Xiaoli Shi, Fan Wu, Mingdong Ren, Guang Gao, Qinglong Wu
BMC Genomics.2020;[Epub] CrossRef
Small heat shock protein genes of the green algae Closterium ehrenbergii: Cloning and differential expression under heat and heavy metal stresses
Sofia Abassi, Hui Wang, Thangaraj Ponmani, Jang‐Seu Ki
Environmental Toxicology.2019; 34(9): 1013. CrossRef
Yellow clay modulates carbohydrate and glutathione responses in the harmful dinoflagellate Cochlodinium polykrikoides and leads to sedimentation
Jang-Seu Ki, Vinitha Ebenezer, Weol-Ae Lim
European Journal of Protistology.2019; 71: 125642. CrossRef
Differential transcriptional responses of carotenoid biosynthesis genes in the marine green alga Tetraselmis suecica exposed to redox and non-redox active metals
Ramaraj Sathasivam, Jang-Seu Ki
Molecular Biology Reports.2019; 46(1): 1167. CrossRef
Origin and roles of a novel copper-zinc superoxide dismutase (CuZnSOD) gene from the harmful dinoflagellate Prorocentrum minimum
Hui Wang, Sofia Abassi, Jang-Seu Ki
Gene.2019; 683: 113. CrossRef
Enhancement of growth and paramylon production of Euglena gracilis by co-cultivation with Pseudoalteromonas sp. MEBiC 03485
Min Seo Jeon, Jeong-Joo Oh, Jee Young Kim, Sang-Il Han, Sang Jun Sim, Yoon-E Choi
Bioresource Technology.2019; 288: 121513. CrossRef
Molecular cloning and oxidative-stress responses of a novel manganese superoxide dismutase (MnSOD) gene in the dinoflagellate Prorocentrum minimum
Hui Wang, Hansol Kim, Weol-Ae Lim, Jang-Seu Ki
Molecular Biology Reports.2019; 46(6): 5955. CrossRef

Hydrogen sulfide inhibits the growth of Escherichia coli through oxidative damage: Liu-Hui Fu , Zeng-Zheng Wei , Kang-Di Hu , Lan-Ying Hu , Yan-Hong Li , Xiao-Yan Chen , Zhuo Han , Gai-Fang Yao , Hua Zhang; J. Microbiol. 2018;56(4):238-245. Published online February 28, 2018; DOI: https://doi.org/10.1007/s12275-018-7537-1

62 View
0 Download
62 Crossref

Abstract

Many studies have shown that hydrogen sulfide (H2S) is both detrimental and beneficial to animals and plants, whereas its effect on bacteria is not fully understood. Here, we report that H2S, released by sodium hydrosulfide (NaHS), significantly inhibits the growth of Escherichia coli in a dose-dependent manner. Further studies have shown that H2S treatment stimulates the production of reactive oxygen species (ROS) and decreases glutathione (GSH) levels in E. coli, resulting in lipid peroxidation and DNA damage. H2S also inhibits the antioxidative enzyme activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) and induces the response of the SoxRS and OxyR regulons in E. coli. Moreover, pretreatment with the antioxidant ascorbic acid (AsA) could effectively prevent H2S-induced toxicity in E. coli. Taken together, our results indicate that H2S exhibits an antibacterial effect on E. coli through oxidative damage and suggest a possible application for H2S in water and food processing.

Citations

Citations to this article as recorded by

Resensitization of Multi Drug-Resistant Aeromonas caviae with Exogenous Hydrogen Sulfide Potentiated Antibiotics
Sahithya Selvakumar, Shubhi Singh, Priya Swaminathan
Current Microbiology.2025;[Epub] CrossRef
Metagenomics and metatranscriptomics insights into microbial enhancement of H2S removal and CO2 assimilation
Junjie Wang, Zhuowei Cheng, Yunfei Su, Jiade Wang, Dongzhi Chen, Jianmeng Chen, Xiaoming Wu, Aobo Chen, Zhenyu Gu
Journal of Environmental Management.2025; 373: 123714. CrossRef
Transcriptional memories mediate the plasticity of sulfide stress responses to enable acclimation in Urechis unicinctus
Wenqing Zhang, Danwen Liu, Heran Yang, Tianya Yang, Zhifeng Zhang, Yubin Ma
Ecotoxicology and Environmental Safety.2025; 293: 118020. CrossRef
Harnessing high-level hydrogen sulfide stress for enhanced biogas utilization: Adaptive resilience of a mixed-culture system
Baorui Zhang, Jianbo Liu, Chen Cai, Yan Zhou
Chemical Engineering Journal.2025; 506: 160300. CrossRef
Deep-sea in situ and laboratory multi-omics provide insights into the sulfur assimilation of a deep-sea Chloroflexota bacterium
Rikuan Zheng, Chong Wang, Chaomin Sun, Christa M. Schleper
mBio.2024;[Epub] CrossRef
Detection and evaluation of susceptibility to antibiotics in non-hydrogen sulfide-producing antibiotic-resistant soil microbe: Pseudomonas guariconensis
Sahithya Selvakumar, Shubhi Singh, Priya Swaminathan
International Microbiology.2024;[Epub] CrossRef
Nanomaterials‐Induced Redox Imbalance: Challenged and Opportunities for Nanomaterials in Cancer Therapy
Xumeng Wu, Ziqi Zhou, Kai Li, Shaoqin Liu
Advanced Science.2024;[Epub] CrossRef
pH-Responsive nanoplatform synergistic gas/photothermal therapy to eliminate biofilms in poly(l-lactic acid) scaffolds
Guowen Qian, Yuqian Mao, Huihui Zhao, Lemin Zhang, Long Xiong, Zhisheng Long
Journal of Materials Chemistry B.2024; 12(5): 1379. CrossRef
Enhancing cancer treatment via “Zn2+ interference” with Zn-based nanomaterials
Yandong Wang, Fucheng Gao, Li Zhao, Yanqiu Wu, Can Li, Hui Li, Yanyan Jiang
Coordination Chemistry Reviews.2024; 500: 215535. CrossRef
Effect of H2S and cysteine homeostasis disturbance on ciprofloxacin sensitivity of Escherichia coli in cystine-free and cystine-fed minimal medium
Galina Smirnova, Aleksey Tyulenev, Lyubov Sutormina, Tatyana Kalashnikova, Zoya Samoilova, Nadezda Muzyka, Vadim Ushakov, Oleg Oktyabrsky
Archives of Microbiology.2024;[Epub] CrossRef
Identification of the organic peroxide scavenging system of Yersinia pseudotuberculosis and its regulation by OxyR
Junfeng Fan, Xiaofen Mo, Hui Zhang, Linna Xu, Jianhua Yin, Fen Wan, Nicole R. Buan
Applied and Environmental Microbiology.2024;[Epub] CrossRef
Microenvironment Responsive Biomineralization Nanofirework Employing H2S-Assisted Photothermal Therapy to Prompt Bacterial Wound Healing
Xianan Li, Hairui Deng, Lingfeng Pan, Ziyue Xu, Mengcheng Tang, Zhimin He, Yachen Xu, Hao Fu, Ruibo Zhao, Shibo Wang, Xiangdong Kong
ACS Applied Nano Materials.2024; 7(17): 20678. CrossRef
H2S scavenger as a broad-spectrum strategy to deplete bacteria-derived H2S for antibacterial sensitization
Jiekai Sun, Xu Wang, Ye Gao, Shuangyu Li, Ziwei Hu, Yan Huang, Baoqiang Fan, Xia Wang, Miao Liu, Chunhua Qiao, Wei Zhang, Yipeng Wang, Xingyue Ji
Nature Communications.2024;[Epub] CrossRef
Fabricating a PDA-NOate@CuS coated NIR-activatable titanium implant to realize simultaneous antiinfection and osseointegration
Jiahuan Liu, Xiaowan Li, Shangyu Xie, Ruirui Ma, Hongfei Wang, Shurong Ban, Chengwu Zhang, Lixia Guo
New Journal of Chemistry.2024; 48(25): 11465. CrossRef
Unraveling the potential of hydrogen sulfide as a signaling molecule for plant development and environmental stress responses: A state-of-the-art review
Siloni Singh Bhadwal, Shagun Verma, Shahnawaz Hassan, Satwinderjeet Kaur
Plant Physiology and Biochemistry.2024; 212: 108730. CrossRef
Gas Therapy: Generating, Delivery, and Biomedical Applications
Pejman Ghaffari‐Bohlouli, Hafez Jafari, Oseweuba Valentine Okoro, Houman Alimoradi, Lei Nie, Guohua Jiang, Ashok Kakkar, Amin Shavandi
Small Methods.2024;[Epub] CrossRef
Methylosinus trichosporium OB3b drives composition-independent application of biogas in poly(3-hydroxybutyrate) synthesis
Sunho Park, Shinhyeong Choe, Hyejeong Lee, Jaewook Myung
Fuel.2024; 378: 132730. CrossRef
Ahp deficiency-induced redox imbalance leads to metabolic alterations in E. coli
Feng Liu, Penggang Han, Nuomin Li, Yongqian Zhang
Redox Biology.2023; 67: 102888. CrossRef
Effect of sulfamethazine on the horizontal transfer of plasmid-mediated antibiotic resistance genes and its mechanism of action
Xiaojing Yan, Wenwen Liu, Shengfang Wen, Lanjun Wang, Lusheng Zhu, Jun Wang, Young Mo Kim, Jinhua Wang
Journal of Environmental Sciences.2023; 127: 399. CrossRef
D-cysteine desulfhydrase DCD1 participates in tomato resistance against Botrytis cinerea by modulating ROS homeostasis
Yuqi Zhao, Kangdi Hu, Gaifang Yao, Siyue Wang, Xiangjun Peng, Conghe Zhang, Dexin Zeng, Kai Zong, Yaning Lyu, Hua Zhang
Vegetable Research.2023;[Epub] CrossRef
Bacteria‐Targeted Combined with Photothermal/NO Nanoparticles for the Treatment and Diagnosis of MRSA Infection In Vivo
Kai Lv, Guowei Li, Xiangjun Pan, Luxuan Liu, Ziheng Chen, Yu Zhang, Hao Xu, Dong Ma
Advanced Healthcare Materials.2023;[Epub] CrossRef
Antibiotic Resistance: Challenges and Strategies in Combating Infections
Jay Chavada, Komal N Muneshwar, Yash Ghulaxe, Mohit Wani, Prayas P Sarda, Shreyash Huse
Cureus.2023;[Epub] CrossRef
Enhancement of bio-S0 recovery and revealing the inhibitory effect on microorganisms under high sulfide loading
Junjie Wang, Zhuowei Cheng, Jiade Wang, Dongzhi Chen, Jianmeng Chen, Jianming Yu, Songkai Qiu, Dionysios D. Dionysiou
Environmental Research.2023; 238: 117214. CrossRef
Enzyme‐Triggered Chemodynamic Therapy via a Peptide‐H2S Donor Conjugate with Complexed Fe2+
Yumeng Zhu, William R. Archer, Katlyn F. Morales, Michael D. Schulz, Yin Wang, John B. Matson
Angewandte Chemie.2023;[Epub] CrossRef
Antibacterial gas therapy: Strategies, advances, and prospects
Tian-Yu Wang, Xiao-Yu Zhu, Fu-Gen Wu
Bioactive Materials.2023; 23: 129. CrossRef
Reactive oxygen species-upregulating nanomedicines towards enhanced cancer therapy
Yuanyuan Ding, Qingqing Pan, Wenxia Gao, Yuji Pu, Kui Luo, Bin He
Biomaterials Science.2023; 11(4): 1182. CrossRef
Chameleon-like Anammox Bacteria for Surface Color Change after Suffering Starvation
Jingqi Sun, Yiming Feng, Ru Zheng, Lingrui Kong, Xiaogang Wu, Kuo Zhang, Jianhang Zhou, Sitong Liu
Environmental Science & Technology.2023; 57(40): 15087. CrossRef
The Triple Crown: NO, CO, and H2S in cancer cell biology
Palak P. Oza, Khosrow Kashfi
Pharmacology & Therapeutics.2023; 249: 108502. CrossRef
Stability and biomineralization of cadmium sulfide nanoparticles biosynthesized by the bacterium Rhodopseudomonas palustris under light
Su-Fang Xing, Hui-Fang Tian, Zhen Yan, Chao Song, Shu-Guang Wang
Journal of Hazardous Materials.2023; 458: 131937. CrossRef
Intelligent polymeric hydrogen sulfide delivery systems for therapeutic applications
Fan Rong, Tengjiao Wang, Qian Zhou, Haowei Peng, Jingtian Yang, Quli Fan, Peng Li
Bioactive Materials.2023; 19: 198. CrossRef
Nanoplatform-based cellular reactive oxygen species regulation for enhanced oncotherapy and tumor resistance alleviation
Meifang Wang, Ping'an Ma, Jun Lin
Chinese Chemical Letters.2023; 34(9): 108300. CrossRef
In situ formation of ferrous sulfide in glycyrrhizic acid hydrogels to promote healing of multi-drug resistant Staphylococcus aureus-infected diabetic wounds
Zhuobin Xu, Ze Xu, Jiake Gu, Juan Zhou, Gengyu Sha, Ying Huang, Tong Wang, Lei Fan, Yanfeng Zhang, Juqun Xi
Journal of Colloid and Interface Science.2023; 650: 1918. CrossRef
The Conditions Matter: The Toxicity of Titanium Trisulfide Nanoribbons to Bacteria E. coli Changes Dramatically Depending on the Chemical Environment and the Storage Time
Olga V. Zakharova, Valeria V. Belova, Peter A. Baranchikov, Anna A. Kostyakova, Dmitry S. Muratov, Gregory V. Grigoriev, Svetlana P. Chebotaryova, Denis V. Kuznetsov, Alexander A. Gusev
International Journal of Molecular Sciences.2023; 24(9): 8299. CrossRef
Community ecological study on the reduction of soil antimony bioavailability by SRB-based remediation technologies
Min Zhang, Jing Xiong, Lei Zhou, Jingjing Li, Jianqiang Fan, Xing Li, Teng Zhang, Zhuzhong Yin, Huaqun Yin, Xueduan Liu, Delong Meng
Journal of Hazardous Materials.2023; 459: 132256. CrossRef
Enzyme‐Triggered Chemodynamic Therapy via a Peptide‐H2S Donor Conjugate with Complexed Fe2+
Yumeng Zhu, William R. Archer, Katlyn F. Morales, Michael D. Schulz, Yin Wang, John B. Matson
Angewandte Chemie International Edition.2023;[Epub] CrossRef
Enhancement of dissimilatory nitrate/nitrite reduction to ammonium of Escherichia coli sp. SZQ1 by ascorbic acid: Mechanism and performance
Zhiqiang Su, Yu Zhang, Ruizhi Zhao, Jiti Zhou
Science of The Total Environment.2022; 853: 158423. CrossRef
Mitochondria-targeting Type I AIE photosensitizer combined with H2S therapy: Uninterrupted hydroxyl radical generation for enhancing tumor therapy
Tianfu Zhang, Zeming Liu, Wenxue Tang, Daoming Zhu, Meng Lyu, Jacky Wing Yip Lam, Qinqin Huang, Ben Zhong Tang
Nano Today.2022; 46: 101620. CrossRef
Generation and Physiology of Hydrogen Sulfide and Reactive Sulfur Species in Bacteria
Sirui Han, Yingxi Li, Haichun Gao
Antioxidants.2022; 11(12): 2487. CrossRef
Metal sulfide precipitation mediated by an elemental sulfur-reducing thermoacidophilic microbial culture from a full-scale anaerobic reactor
Adrian Hidalgo-Ulloa, Cees Buisman, Jan Weijma
Hydrometallurgy.2022; 213: 105950. CrossRef
Oxidative stress response system in Escherichia coli arising from diphenyl ditelluride (PhTe)2 exposure
F.C. Pinheiro, V.C. Bortolotto, S.M. Araujo, S.F. Couto, M.M.M. Dahleh, M. Cancela, J. Neto, G. Zeni, A. Zaha, M. Prigol
Toxicology in Vitro.2022; 83: 105404. CrossRef
On-demand therapeutic delivery of hydrogen sulfide aided by biomolecules
Yuxuan Ge, Fan Rong, Wei Li, Yin Wang
Journal of Controlled Release.2022; 352: 586. CrossRef
Antibiotic resistance: The challenges and some emerging strategies for tackling a global menace
David Chinemerem Nwobodo, Malachy Chigozie Ugwu, Clement Oliseloke Anie, Mushtak T. S. Al‐Ouqaili, Joseph Chinedu Ikem, Uchenna Victor Chigozie, Morteza Saki
Journal of Clinical Laboratory Analysis.2022;[Epub] CrossRef
Vitamin C Maintenance against Cell Growth Arrest and Reactive Oxygen Species Accumulation in the Presence of Redox Molecular Chaperone hslO Gene
Akihiro Kaidow, Noriko Ishii, Shingo Suzuki, Takashi Shiina, Hirokazu Kasahara
International Journal of Molecular Sciences.2022; 23(21): 12786. CrossRef
Cysteine Biosynthesis in Campylobacter jejuni: Substrate Specificity of CysM and the Dualism of Sulfide
Noah Hitchcock, David J. Kelly, Andrew Hitchcock, Aidan J. Taylor
Biomolecules.2022; 13(1): 86. CrossRef
Transcriptomic analysis of chloride tolerance in Leptospirillum ferriphilum DSM 14647 adapted to NaCl
Javier Rivera-Araya, Thomas Heine, Renato Chávez, Michael Schlömann, Gloria Levicán, Benjamin J. Koestler
PLOS ONE.2022; 17(4): e0267316. CrossRef
Acute stress of the typical disinfectant glutaraldehyde-didecyldimethylammonium bromide (GD) on sludge microecology in livestock wastewater treatment plants: Effect and its mechanisms
Yuxin Li, Jiayin Ling, Jinghao Xue, Junwei Huang, Xiao Zhou, Fei Wang, Waner Hou, Jianbin Zhao, Yanbin Xu
Water Research.2022; 227: 119342. CrossRef
Sulfide Treatment Alters Antioxidant Response and Related Genes Expressions in Rice Field Eel (Monopterus albus)
Liqiao Zhong, Fan Yao, He Zhang, Huaxiao Xie, Huijun Ru, Nian Wei, Zhaohui Ni, Zhong Li, Yunfeng Li
Water.2022; 14(20): 3230. CrossRef
Development of Polycaprolactone–Zeolite Nanoporous Composite Films for Topical Therapeutic Release of Different Gasotransmitters
Rosana V. Pinto, Sílvia Carvalho, Fernando Antunes, João Pires, Moisés L. Pinto
ACS Applied Nano Materials.2022; 5(7): 9230. CrossRef
Near-infrared laser-controlled nitric oxide-releasing gold nanostar/hollow polydopamine Janus nanoparticles for synergistic elimination of methicillin-resistant Staphylococcus aureus and wound healing
Zhuoying Liang, Wenkang Liu, Ziqiang Wang, Peilian Zheng, Wei Liu, Jianfu Zhao, Yunlong Zhong, Yan Zhang, Jing Lin, Wei Xue, Siming Yu
Acta Biomaterialia.2022; 143: 428. CrossRef
Cysteine supplementation enhanced inhibitor tolerance of Zymomonas mobilis for economic lignocellulosic bioethanol production
Xiongying Yan, Xia Wang, Yongfu Yang, Zhen Wang, Haoyu Zhang, Yang Li, Qiaoning He, Mian Li, Shihui Yang
Bioresource Technology.2022; 349: 126878. CrossRef
Natural inactivation of MS2, poliovirus type 1 and Cryptosporidium parvum in an anaerobic and reduced aquifer
John T. Lisle, George Lukasik
Journal of Applied Microbiology.2022; 132(3): 2464. CrossRef
Hydrogen sulfide in longevity and pathologies: Inconsistency is malodorous
Alexander S. Sokolov, Pavel V. Nekrasov, Mikhail V. Shaposhnikov, Alexey A. Moskalev
Ageing Research Reviews.2021; 67: 101262. CrossRef
Hydrogen Sulfide and Carbon Monoxide Tolerance in Bacteria
Sofia S. Mendes, Vanessa Miranda, Lígia M. Saraiva
Antioxidants.2021; 10(5): 729. CrossRef
CBS-derived H2S facilitates host colonization of Vibrio cholerae by promoting the iron-dependent catalase activity of KatB
Yao Ma, Xiaoman Yang, Hongou Wang, Zixin Qin, Chunrong Yi, Changping Shi, Mei Luo, Guozhong Chen, Jin Yan, Xiaoyun Liu, Zhi Liu, William Navarre
PLOS Pathogens.2021; 17(7): e1009763. CrossRef
Hydrogen sulfide (H2S) signaling in plant development and stress responses
Hai Liu, Jicheng Wang, Jianhao Liu, Tong Liu, Shaowu Xue
aBIOTECH.2021; 2(1): 32. CrossRef
Hydrogen sulfide: An endogenous regulator of the immune system
Nahzli Dilek, Andreas Papapetropoulos, Tracy Toliver-Kinsky, Csaba Szabo
Pharmacological Research.2020; 161: 105119. CrossRef
Protective Role of Bacterial Alkanesulfonate Monooxygenase under Oxidative Stress
Chulwoo Park, Bora Shin, Woojun Park, Maia Kivisaar
Applied and Environmental Microbiology.2020;[Epub] CrossRef
Hydrogen Sulfide Sensitizes Acinetobacter baumannii to Killing by Antibiotics
Say Yong Ng, Kai Xun Ong, Smitha Thamarath Surendran, Ameya Sinha, Joey Jia Hui Lai, Jacqueline Chen, Jiaqi Liang, Leona Kwan Sing Tay, Liang Cui, Hooi Linn Loo, Peiying Ho, Jongyoon Han, Wilfried Moreira
Frontiers in Microbiology.2020;[Epub] CrossRef
FeS@BSA Nanoclusters to Enable H2S‐Amplified ROS‐Based Therapy with MRI Guidance
Congkun Xie, Dong Cen, Zhaohui Ren, Yifan Wang, Yongjun Wu, Xiang Li, Gaorong Han, Xiujun Cai
Advanced Science.2020;[Epub] CrossRef
Hydrogen sulfide and environmental stresses
John T. Hancock
Environmental and Experimental Botany.2019; 161: 50. CrossRef
The H2S Donor GYY4137 Stimulates Reactive Oxygen Species Generation in BV2 Cells While Suppressing the Secretion of TNF and Nitric Oxide
Milica Lazarević, Emanuela Mazzon, Miljana Momčilović, Maria Sofia Basile, Giuseppe Colletti, Maria Cristina Petralia, Placido Bramanti, Ferdinando Nicoletti, Đorđe Miljković
Molecules.2018; 23(11): 2966. CrossRef
Do nitric oxide, carbon monoxide and hydrogen sulfide really qualify as ‘gasotransmitters’ in bacteria?
Lauren K. Wareham, Hannah M. Southam, Robert K. Poole
Biochemical Society Transactions.2018; 46(5): 1107. CrossRef

First
Prev
Page of 4
Next
Last

Search