Journal of Microbiology

Research Support, Non-U.S. Gov'ts

Antifungal activity of violacein purified from a novel strain of Chromobacterium sp. NIIST (MTCC 5522): Anju Sasidharan , Nishanth Kumar Sasidharan , Dileepkumar Bhaskaran Nair Saraswathy Amma , Radhakrishnan Kokkuvayil Vasu , Anupama Vijaya Nataraja , Krishnakumar Bhaskaran; J. Microbiol. 2015;53(10):694-701. Published online October 2, 2015; DOI: https://doi.org/10.1007/s12275-015-5173-6

65 View
0 Download
44 Crossref

A novel strain of Chromobacterium sp. NIIST (MTCC 5522) producing high level of purple blue bioactive compound violacein was isolated from clay mine acidic sediment. During 24 h aerobic incubation in modified Luria Bertani medium, around 0.6 g crude violacein was produced per gram of dry weight biomass. An inexpensive method for preparing crystalline, pure violacein from crude pigment was developed (12.8 mg violacein/L) and the pure compound was characterized by different spectrometric methods. The violacein prepared was found effective against a number of plant and human pathogenic fungi and yeast species such as Cryptococcus gastricus, Trichophyton rubrum, Fusarium oxysporum, Rhizoctonia solani, Aspergillus flavus, Penicillium expansum, and Candida albicans. The best activity was recorded against Trichophyton rubrum (2 μg/ml), a human pathogen responsible for causing athlete’s foot infection. This is the first report of antifungal activity of purified violacein against pathogenic fungi and yeast.

Citations

Citations to this article as recorded by

Mechanistic Insight of Pharmacological Aspects of Violacein: Recent Trends and Advancements
Abhishek Chauhan, Darin Mansor Mathkor, Hemant Joshi, Ritu Chauhan, Ujjawal Sharma, Vikas Sharma, Manoj Kumar, Reena V. Saini, Adesh K. Saini, Hardeep Singh Tuli, Damandeep Kaur, Shafiul Haque
Journal of Biochemical and Molecular Toxicology.2025;[Epub] CrossRef
Extracellular vesicles of Janthinobacterium lividum as violacein carriers in melanoma cell treatment
Patrycja Kowalska, Jolanta Mierzejewska, Paulina Skrzeszewska, Aleksandra Witkowska, Katarzyna Oksejuk, Ewa Sitkiewicz, Mariusz Krawczyk, Magdalena Świadek, Agata Głuchowska, Klaudia Marlicka, Anna Sobiepanek, Małgorzata Milner-Krawczyk
Applied Microbiology and Biotechnology.2024;[Epub] CrossRef
Chromobacterium indicum sp. nov., a Pigment-Producing Bacterium Isolated from Soil
Nandini Verma, Stanzin Choksket, Riya Singla, Anil Kumar Pinnaka, Suresh Korpole
Current Microbiology.2024;[Epub] CrossRef
AHL-Based Quorum Sensing Regulates the Biosynthesis of a Variety of Bioactive Molecules in Bacteria
Mélanie Gonzales, Pauline Jacquet, Floriane Gaucher, Éric Chabrière, Laure Plener, David Daudé
Journal of Natural Products.2024; 87(4): 1268. CrossRef
Gaining molecular insights towards inhibition of foodborne fungi Aspergillus fumigatus by a food colourant violacein via computational approach
R. Sindhu, Smitha S. Bhat, Jiraporn Sangta, Chandan Dharmashekar, Bhargav Shreevatsa, Chandan Shivamallu, Devananda Devegowda, Shiva Prasad Kollur, Sheikh F. Ahmad, Sabry M. Attia, Sarana Rose Sommano, Shashanka K. Prasad
Scientific Reports.2024;[Epub] CrossRef
New Target Gene Screening Using Shortened and Random sgRNA Libraries in Microbial CRISPR Interference
Song Hee Jeong, Hyun Ju Kim, Sang Jun Lee
ACS Synthetic Biology.2023; 12(3): 800. CrossRef
Natural Pigments Production and Their Application in Food, Health and Other Industries
Eleonora Di Salvo, Giovanna Lo Vecchio, Rita De Pasquale, Laura De Maria, Roberta Tardugno, Rossella Vadalà, Nicola Cicero
Nutrients.2023; 15(8): 1923. CrossRef
Antifungal Compounds from Microbial Symbionts Associated with Aquatic Animals and Cellular Targets: A Review
Madeleine Nina Love Ngo-Mback, Elisabeth Zeuko’o Menkem, Heather G. Marco
Pathogens.2023; 12(4): 617. CrossRef
An extensive review of marine pigments: sources, biotechnological applications, and sustainability
Luis Alfonso Muñoz-Miranda, Maricarmen Iñiguez-Moreno
Aquatic Sciences.2023;[Epub] CrossRef
A Simple Characterisation of Violacein Compound Derived from Chromobacterium sp. strain Dyh27s2016 and its Antimicrobial Activity Against Pseudomonas aeruginosa
Pratheep Sandrasaigaran, Mohamed Jafran Abul Baseer
Malaysian Journal of Science and Advanced Technology.2023; : 1. CrossRef
Priming Chromobacterium violaceum as a Gold Bioleacher in E-wastes: Advantages and Challenges
Thiviaraj Palanysamy, Rattika Sukalingam, Pratheep Sandrasaigaran
Malaysian Journal of Science and Advanced Technology.2023; : 222. CrossRef
Purification of Natural Pigments Violacein and Deoxyviolacein Produced by Fermentation Using Yarrowia lipolytica
Georgio Nemer, Nicolas Louka, Paul Rabiller Blandin, Richard G. Maroun, Eugène Vorobiev, Tristan Rossignol, Jean-Marc Nicaud, Erwann Guénin, Mohamed Koubaa
Molecules.2023; 28(11): 4292. CrossRef
Genome sequencing and multifaceted taxonomic analysis of novel strains of violacein-producing bacteria and non-violacein-producing close relatives
Marina Estella De León, Harriet S. Wilson, Guillaume Jospin, Jonathan A. Eisen
Microbial Genomics .2023;[Epub] CrossRef
Fermentation in Minimal Media and Fungal Elicitation Enhance Violacein and Deoxyviolacein Production in Two Janthinobacterium Strains
Andri Frediansyah, Yosephine Sri Wulan Manuhara, Alfinda Novi Kristanti, Arif Luqman, Anjar Tri Wibowo
Fermentation.2022; 8(12): 714. CrossRef
Improvement in Violacein Production by Utilizing Formic Acid to Induce Quorum Sensing in Chromobacterium violaceum
Kuan-Chen Cheng, Hsiang-Chun Hsiao, Yu-Chen Hou, Chang-Wei Hsieh, Hsien-Yi Hsu, Hung-Yueh Chen, Shin-Ping Lin
Antioxidants.2022; 11(5): 849. CrossRef
Violacein from an Antarctic Iodobacter sp. 7MAnt and its function as immunomodulator of the defence mechanism of innate immunity in fish cells
Beatriz Valenzuela, Almendra Benavides, Daniela Gutierrez, Lotsé Blamey, María Teresa Monsalves, Brenda Modak, Jenny M. Blamey
Journal of Fish Diseases.2022; 45(3): 485. CrossRef
Fermentation Quality and Microbial Community of Corn Stover or Rice Straw Silage Mixed with Soybean Curd Residue
Xiaolin Wang, Jiamei Song, Zihan Liu, Guangning Zhang, Yonggen Zhang
Animals.2022; 12(7): 919. CrossRef
Development of Methylorubrum extorquens AM1 as a promising platform strain for enhanced violacein production from co-utilization of methanol and acetate
Hoa Thi Quynh Le, Dung Hoang Anh Mai, Jeong-Geol Na, Eun Yeol Lee
Metabolic Engineering.2022; 72: 150. CrossRef
Prey identity affects fitness of a generalist consumer in a brown food web
Lily Khadempour, Leslie Rivas Quijano, Casey P. terHorst
Ecology and Evolution.2022;[Epub] CrossRef
Violacein and its antifungal activity: comments and potentialities
N. Durán, G.R. Castro, R.W.D. Portela, W.J. Fávaro, M. Durán, L. Tasic, G. Nakazato
Letters in Applied Microbiology.2022; 75(4): 796. CrossRef
Applications of Natural Violet Pigments from Halophilic Chromobacterium violaceum PDF23 for Textile Dyeing with Antimicrobial and Antioxidant Potentials
Antonyraj Matharasi Perianaika Anahas, Subramanian Kumaran, Mahmoud Kandeel, Gangatharan Muralitharan, Jenifer Silviya, Geja Lakshmi Adhimoolam, Mani Panagal, Sampath Renuka Pugazhvendan, Gopal Suresh, A. Wilson Aruni, Senthil Rethinam, Nainangu Prasannab
Journal of Nanomaterials.2022;[Epub] CrossRef
Evaluation of the anti‑proliferative activity of violacein, a natural pigment of bacterial origin, in urinary bladder cancer cell lines
Bruna Neron, Maria Zingaropoli, Giulia Radocchia, Maria Ciardi, Luciana Mosca, Fabrizio Pantanella, Serena Schippa
Oncology Letters.2022;[Epub] CrossRef
Isolation and Properties of the Bacterial Strain Janthinobacterium sp. SLB01
Lubov Chernogor, Kseniya Bakhvalova, Alina Belikova, Sergei Belikov
Microorganisms.2022; 10(5): 1071. CrossRef
Bacterial pigments: A colorful palette reservoir for biotechnological applications
Viviana Teresa Orlandi, Eleonora Martegani, Cristina Giaroni, Andreina Baj, Fabrizio Bolognese
Biotechnology and Applied Biochemistry.2022; 69(3): 981. CrossRef
Lignocellulosic substrates as starting materials for the production of bioactive biopigments
Tiago Daniel Madureira de Medeiros, Laurent Dufossé, Juliano Lemos Bicas
Food Chemistry: X.2022; 13: 100223. CrossRef
Biotechnological Activities and Applications of Bacterial Pigments Violacein and Prodigiosin
Seong Yeol Choi, Sungbin Lim, Kyoung-hye Yoon, Jin I. Lee, Robert J. Mitchell
Journal of Biological Engineering.2021;[Epub] CrossRef
Multi-target drug with potential applications: violacein in the spotlight
Nelson Durán, Gerson Nakazato, Marcela Durán, Ignasio R. Berti, Guillermo R. Castro, Danijela Stanisic, Marcelo Brocchi, Wagner J. Fávaro, Carmen V. Ferreira-Halder, Giselle Z. Justo, Ljubica Tasic
World Journal of Microbiology and Biotechnology.2021;[Epub] CrossRef
Chemical signals driving bacterial–fungal interactions
Saima Khalid, Nancy P. Keller
Environmental Microbiology.2021; 23(3): 1334. CrossRef
Disrupting quorum sensing alters social interactions in Chromobacterium violaceum
Sonia Mion, Nathan Carriot, Julien Lopez, Laure Plener, Annick Ortalo-Magné, Eric Chabrière, Gérald Culioli, David Daudé
npj Biofilms and Microbiomes.2021;[Epub] CrossRef
Recent Advances in Synthetic, Industrial and Biological Applications of Violacein and Its Heterologous Production
Aqsa Ahmed, Abdullah Ahmad, Renhan Li, Waleed AL-Ansi, Momal Fatima, Bilal Sajid Mushtaq, Samra Basharat, Ye Li, Zhonghu Bai
Journal of Microbiology and Biotechnology.2021; 31(11): 1465. CrossRef
Microbial synthesis of violacein pigment and its potential applications
HyunA Park, SeoA Park, Yung-Hun Yang, Kwon-Young Choi
Critical Reviews in Biotechnology.2021; 41(6): 879. CrossRef
Exploitation of Versatile Naturally Derived Blue Colorant Pigment 3-[2-hydroxy-5-(5-hydroxy-1H-indol-3-yl)-1H-pyrrol-3-yl] indol-one
Ishani, Isita, T. Vijayakumar
IOP Conference Series: Materials Science and Engineering.2021; 1219(1): 012020. CrossRef
Characterization and low-cost preservation of Chromobacterium violaceum strain TRFM-24 isolated from Tripura state, India
Sushil K. Sharma, Rakhi Dhyani, Ees Ahmad, Pankaj K. Maurya, Madhu Yadav, Ramesh Chandra Yadav, Vinod Kumar Yadav, Pawan K. Sharma, Mahaveer P. Sharma, Aketi Ramesh, Anil K. Saxena
Journal of Genetic Engineering and Biotechnology.2021; 19(1): 146. CrossRef
Production and antiproliferative effect of violacein, a purple pigment produced by an Antarctic bacterial isolate
Diego Alem, Juan José Marizcurrena, Verónica Saravia, Danilo Davyt, Wilner Martinez-Lopez, Susana Castro-Sowinski
World Journal of Microbiology and Biotechnology.2020;[Epub] CrossRef
An Overview on Industrial and Medical Applications of Bio-Pigments Synthesized by Marine Bacteria
Ali Nawaz, Rida Chaudhary, Zinnia Shah, Laurent Dufossé, Mireille Fouillaud, Hamid Mukhtar, Ikram ul Haq
Microorganisms.2020; 9(1): 11. CrossRef
Isolation and characterization of violacein from an Antarctic Iodobacter: a non-pathogenic psychrotolerant microorganism
Joaquin Atalah, Lotsé Blamey, Sebastian Muñoz-Ibacache, Felipe Gutierrez, Marcela Urzua, Maria Victoria Encinas, Maritza Páez, Junsong Sun, Jenny M. Blamey
Extremophiles.2020; 24(1): 43. CrossRef
Interplay between two quorum sensing‐regulated pathways, violacein biosynthesis and VacJ/Yrb, dictates outer membrane vesicle biogenesis in Chromobacterium violaceum
Juliana H. Batista, Fernanda C. Leal, Taise T. H. Fukuda, Juliana Alcoforado Diniz, Fausto Almeida, Mônica T. Pupo, José F. da Silva Neto
Environmental Microbiology.2020; 22(6): 2432. CrossRef
Violacein Targets the Cytoplasmic Membrane of Bacteria
Ana C. G. Cauz, Gustavo P. B. Carretero, Greice K. V. Saraiva, Peter Park, Laura Mortara, Iolanda M. Cuccovia, Marcelo Brocchi, Frederico J. Gueiros-Filho
ACS Infectious Diseases.2019; 5(4): 539. CrossRef
Optoregulated Drug Release from an Engineered Living Material: Self‐Replenishing Drug Depots for Long‐Term, Light‐Regulated Delivery
Shrikrishnan Sankaran, Judith Becker, Christoph Wittmann, Aránzazu del Campo
Small.2019;[Epub] CrossRef
Antiplasmodial and trypanocidal activity of violacein and deoxyviolacein produced from synthetic operons
Elizabeth Bilsland, Tatyana A. Tavella, Renata Krogh, Jamie E. Stokes, Annabelle Roberts, James Ajioka, David R. Spring, Adriano D. Andricopulo, Fabio T. M. Costa, Stephen G. Oliver
BMC Biotechnology.2018;[Epub] CrossRef
Stimulation of Violacein Biosynthesis in Chromobacterium violaceum Biofilms in the Presence of Dimethyl Sulfoxide
S. V. Mart’yanov, A. V. Letarov, P. A. Ivanov, V. K. Plakunov
Microbiology.2018; 87(3): 437. CrossRef
Investigating the potential use of an Antarctic variant of Janthinobacterium lividum for tackling antimicrobial resistance in a One Health approach
Andreea Baricz, Adela Teban, Cecilia Maria Chiriac, Edina Szekeres, Anca Farkas, Maria Nica, Amalia Dascălu, Corina Oprișan, Paris Lavin, Cristian Coman
Scientific Reports.2018;[Epub] CrossRef
Interaction of violacein in models for cellular membranes: Regulation of the interaction by the lipid composition at the air-water interface
Karine Damaceno de Souza, Katia Regina Perez, Nelson Durán, Giselle Zenker Justo, Luciano Caseli
Colloids and Surfaces B: Biointerfaces.2017; 160: 247. CrossRef
Fungal and Bacterial Pigments: Secondary Metabolites with Wide Applications
Manik Prabhu Narsing Rao, Min Xiao, Wen-Jun Li
Frontiers in Microbiology.2017;[Epub] CrossRef

Use of Denaturing High-Performance Liquid Chromatography (DHPLC) to Characterize the Bacterial and Fungal Airway Microbiota of Cystic Fibrosis Patients: Jérôme Mounier , Audrey Gouëllo , Marlène Keravec , Solène Le Gal , Grégory Pacini , Stella Debaets , Gilles Nevez , Gilles Rault , Georges Barbier , Geneviève Héry-Arnaud; J. Microbiol. 2014;52(4):307-314. Published online February 17, 2014; DOI: https://doi.org/10.1007/s12275-014-3425-5

58 View
0 Download
8 Crossref

Abstract

The aim of this study was to evaluate the use of denaturing high-performance liquid chromatography (DHPLC) to characterize cystic fibrosis (CF) airway microbiota including both bacteria and fungi. DHPLC conditions were first optimized using a mixture of V6, V7 and V8 region 16S rRNA gene PCR amplicons from 18 bacterial species commonly found in CF patients. Then, the microbial diversity of 4 sputum samples from 4 CF patients was analyzed using cultural methods, cloning/sequencing (for bacteria only) and DHPLC peak fraction collection/sequencing. DHPLC analysis allowed identifying more bacterial and fungal species than the classical culture methods, including well-recognized pathogens such as Pseudomonas aeruginosa. Even if a lower number of bacterial Operational Taxonomic Units (OTUs) was identified by DHPLC, it allowed to find OTUs unidentified by cloning/sequencing. The combination of both techniques permitted to correlate the majority of DHPLC peaks to defined OTUs. Finally, although Aspergillus fumigatus detection using DHPLC can still be improved, this technique clearly allowed to identify a higher number of fungal species versus classical culture-based methods. To conclude, DHPLC provided meaningful additional data concerning pathogenic bacteria and fungi as well as fastidious microorganisms present within the CF respiratory tract. DHPLC can be considered as a complementary technique to culture-dependent analyses in routine microbiological laboratories.

Citations

Citations to this article as recorded by

Chronic Diseases Associated with Malassezia Yeast
Abdourahim Abdillah, Stéphane Ranque
Journal of Fungi.2021; 7(10): 855. CrossRef
The Mycobiome in Health and Disease: Emerging Concepts, Methodologies and Challenges
Pei Yee Tiew, Micheál Mac Aogain, Nur A’tikah Binte Mohamed Ali, Kai Xian Thng, Karlyn Goh, Kenny J. X. Lau, Sanjay H. Chotirmall
Mycopathologia.2020;[Epub] CrossRef
Developing collaborative works for faster progress on fungal respiratory infections in cystic fibrosis
Carsten Schwarz, Patrick Vandeputte, Amandine Rougeron, Sandrine Giraud, Thomas Dugé de Bernonville, Ludovic Duvaux, Amandine Gastebois, Ana Alastruey-Izquierdo, Maria Teresa Martín-Gomez, Estrella Martin Mazuelos, Amparo Sole, Josep Cano, Javier Pemán, G
Medical Mycology.2018; 56(suppl_1): S42. CrossRef
Aspergillus Species in Bronchiectasis: Challenges in the Cystic Fibrosis and Non-cystic Fibrosis Airways
Sanjay H. Chotirmall, Maria Teresa Martin-Gomez
Mycopathologia.2018; 183(1): 45. CrossRef
Fusarium Profiling and Barley Malt Gushing Propensity
Vertti Virkajärvi, Tuija Sarlin, Arja Laitila
Journal of the American Society of Brewing Chemists.2017; 75(3): 181. CrossRef
The lung mycobiome: an emerging field of the human respiratory microbiome
Linh D. N. Nguyen, Eric Viscogliosi, Laurence Delhaes
Frontiers in Microbiology.2015;[Epub] CrossRef
The Airway Colonization by Opportunistic Filamentous Fungi in Patients with Cystic Fibrosis: Recent Updates
Kada Touati, Do Ngoc Linh Nguyen, Laurence Delhaes
Current Fungal Infection Reports.2014; 8(4): 302. CrossRef
Characterization and quantification of the fungal microbiome in serial samples from individuals with cystic fibrosis
Sven D Willger, Sharon L Grim, Emily L Dolben, Anna Shipunova, Thomas H Hampton, Hilary G Morrison, Laura M Filkins, George A O‘Toole, Lisa A Moulton, Alix Ashare, Mitchell L Sogin, Deborah A Hogan
Microbiome.2014;[Epub] CrossRef

Research Support, N.I.H., Extramural

Free Mycolic Acid Accumulation in the Cell Wall of the mce1 Operon Mutant Strain of Mycobacterium tuberculosis: Sally A. Cantrell , Michael D. Leavell , Olivera Marjanovic , Anthony T. Iavarone , Julie A. Leary , Lee W. Riley; J. Microbiol. 2013;51(5):619-626. Published online September 14, 2013; DOI: https://doi.org/10.1007/s12275-013-3092-y

58 View
0 Download
43 Crossref

Abstract

The lipid-rich cell wall of Mycobacterium tuberculosis, the agent of tuberculosis, serves as an effective barrier against many chemotherapeutic agents and toxic host cell effector molecules, and it may contribute to the mechanism of persistence. Mycobacterium tuberculosis strains mutated in a 13-gene operon called mce1, which encodes a putative ABC lipid transporter, induce aberrant granulomatous response in mouse lungs. Because of the postulated role of the mce1 operon in lipid importation, we compared the cell wall lipid composition of wild type and mce1 operon mutant M. tuberculosis H37Rv strains. High resolution mass spectrometric analyses of the mce1 mutant lipid extracts showed unbound mycolic acids to accumulate in the cell wall. Quantitative analysis revealed a 10.7 fold greater amount of free mycolates in the mutant compared to that of the wild type strain. The free mycolates were comprised of alpha, methoxy and keto mycolates in the ratio 1:0.9:0.6, respectively. Since the mce1 operon is regulated in vivo, the free mycolates that accumulate during infection may serve as a barrier for M. tuberculosis against toxic products and contribute to the pathogen’s persistence.

Citations

Citations to this article as recorded by

Antibiotic resistance in Mycobacterium tuberculosis alters tolerance to cell wall-targeting inhibitors
William J Jowsey, Gregory M Cook, Matthew B McNeil
JAC-Antimicrobial Resistance.2024;[Epub] CrossRef
Genomic analysis of Mycobacterium brumae sustains its nonpathogenic and immunogenic phenotype
Chantal Renau-Mínguez, Paula Herrero-Abadía, Paula Ruiz-Rodriguez, Vicente Sentandreu, Eduard Torrents, Álvaro Chiner-Oms, Manuela Torres-Puente, Iñaki Comas, Esther Julián, Mireia Coscolla
Frontiers in Microbiology.2023;[Epub] CrossRef
Mycobacterial mycolic acids trigger inhibitory receptor Clec12A to suppress host immune responses
Naoya Nishimura, Noriyuki Tomiyasu, Shota Torigoe, Satoru Mizuno, Hanako Fukano, Eri Ishikawa, Harutaka Katano, Yoshihiko Hoshino, Kazuhiro Matsuo, Masatomo Takahashi, Yoshihiro Izumi, Takeshi Bamba, Koichi Akashi, Sho Yamasaki
Tuberculosis.2023; 138: 102294. CrossRef
Mce1R of Mycobacterium tuberculosis prefers long-chain fatty acids as specific ligands: a computational study
Dipanwita Maity, Dheeraj Singh, Amitava Bandhu
Molecular Diversity.2023; 27(6): 2523. CrossRef
Structure of an endogenous mycobacterial MCE lipid transporter
James Chen, Alice Fruhauf, Catherine Fan, Jackeline Ponce, Beatrix Ueberheide, Gira Bhabha, Damian C. Ekiert
Nature.2023; 620(7973): 445. CrossRef
Protein target highlights in CASP15: Analysis of models by structure providers
Leila T. Alexander, Janani Durairaj, Andriy Kryshtafovych, Luciano A. Abriata, Yusupha Bayo, Gira Bhabha, Cécile Breyton, Simon G. Caulton, James Chen, Séraphine Degroux, Damian C. Ekiert, Benedikte S. Erlandsen, Peter L. Freddolino, Dominic Gilzer, Chris
Proteins: Structure, Function, and Bioinformatics.2023; 91(12): 1571. CrossRef
Role of Ring6 in the Function of the E. coli MCE Protein LetB
Casey Vieni, Nicolas Coudray, Georgia L. Isom, Gira Bhabha, Damian C. Ekiert
Journal of Molecular Biology.2022; 434(7): 167463. CrossRef
Mycobacterial MCE proteins as transporters that control lipid homeostasis of the cell wall
Laura I. Klepp, Julia Sabio y Garcia, FabianaBigi
Tuberculosis.2022; 132: 102162. CrossRef
Transporters Involved in the Biogenesis and Functionalization of the Mycobacterial Cell Envelope
Mary Jackson, Casey M. Stevens, Lei Zhang, Helen I. Zgurskaya, Michael Niederweis
Chemical Reviews.2021; 121(9): 5124. CrossRef
Trehalose Recycling Promotes Energy-Efficient Biosynthesis of the Mycobacterial Cell Envelope
Amol Arunrao Pohane, Caleb R. Carr, Jaishree Garhyan, Benjamin M. Swarts, M. Sloan Siegrist, Graham F. Hatfull
mBio.2021;[Epub] CrossRef
TREM2 is a receptor for non-glycosylated mycolic acids of mycobacteria that limits anti-mycobacterial macrophage activation
Ei’ichi Iizasa, Yasushi Chuma, Takayuki Uematsu, Mio Kubota, Hiroaki Kawaguchi, Masayuki Umemura, Kenji Toyonaga, Hideyasu Kiyohara, Ikuya Yano, Marco Colonna, Masahiko Sugita, Goro Matsuzaki, Sho Yamasaki, Hiroki Yoshida, Hiromitsu Hara
Nature Communications.2021;[Epub] CrossRef
Pan‐genomic analysis reveals that the evolution of Dietzia species depends on their living habitats
Hui Fang, Jin‐Bo Xu, Yong Nie, Xiao‐Lei Wu
Environmental Microbiology.2021; 23(2): 861. CrossRef
Molecular Cloning, Purification and Characterization of Mce1R of Mycobacterium tuberculosis
Dipanwita Maity, Rajasekhara Reddy Katreddy, Amitava Bandhu
Molecular Biotechnology.2021; 63(3): 200. CrossRef
Synthesis and recycling of the mycobacterial cell envelope
Katherine A Abrahams, Gurdyal S Besra
Current Opinion in Microbiology.2021; 60: 58. CrossRef
Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis
Pooja Asthana, Dhirendra Singh, Jan Skov Pedersen, Mikko J. Hynönen, Ramita Sulu, Abhinandan V. Murthy, Mikko Laitaoja, Janne Jänis, Lee W. Riley, Rajaram Venkatesan
IUCrJ.2021; 8(5): 757. CrossRef
Mycobacterium abscessus biofilms produce an extracellular matrix and have a distinct mycolic acid profile
Anja Dokic, Eliza Peterson, Mario L. Arrieta-Ortiz, Min Pan, Alessandro Di Maio, Nitin Baliga, Apoorva Bhatt
The Cell Surface.2021; 7: 100051. CrossRef
ATP disrupts lipid-binding equilibrium to drive retrograde transport critical for bacterial outer membrane asymmetry
Wen-Yi Low, Shuhua Thong, Shu-Sin Chng
Proceedings of the National Academy of Sciences.2021;[Epub] CrossRef
High-Throughput Screen for Cell Wall Synthesis Network Module in Mycobacterium tuberculosis Based on Integrated Bioinformatics Strategy
Xizi Luo, Jiahui Pan, Qingyu Meng, Juanjuan Huang, Wenfang Wang, Nan Zhang, Guoqing Wang
Frontiers in Bioengineering and Biotechnology.2020;[Epub] CrossRef
LetB Structure Reveals a Tunnel for Lipid Transport across the Bacterial Envelope
Georgia L. Isom, Nicolas Coudray, Mark R. MacRae, Collin T. McManus, Damian C. Ekiert, Gira Bhabha
Cell.2020; 181(3): 653. CrossRef
Differential Host Pro-Inflammatory Response to Mycobacterial Cell Wall Lipids Regulated by the Mce1 Operon
Jéssica D. Petrilli, Igor Müller, Luana E. Araújo, Thiago M. Cardoso, Lucas P. Carvalho, Bruna C. Barros, Maurício Teixeira, Sérgio Arruda, Lee W. Riley, Adriano Queiroz
Frontiers in Immunology.2020;[Epub] CrossRef
Rise of Clinical Microbial Proteogenomics: A Multiomics Approach to Nontuberculous Mycobacterium—The Case ofMycobacterium abscessusUC22
Jayshree Advani, Renu Verma, Oishi Chatterjee, Rex Devasahayam Arokia Balaya, Mohd Altaf Najar, Namitha Ravishankara, Sneha Suresh, Praveen Kumar Pachori, Umesh D. Gupta, Sneha M. Pinto, Devendra S. Chauhan, Srikanth Prasad Tripathy, Harsha Gowda, T.S. Ke
OMICS: A Journal of Integrative Biology.2019; 23(1): 1. CrossRef
Mce-associated protein Rv0177 alters the cell wall structure of Mycobacterium smegmatis and promotes macrophage apoptosis via regulating the cytokines
Shuangquan Yan, Junfeng Zhen, Yue Li, Chenhui Zhang, Andrea Stojkoska, Nzungize Lambert, Qiming Li, Ping Li, Jianping Xie
International Immunopharmacology.2019; 66: 205. CrossRef
Microenvironment ofMycobacterium smegmatisCulture to Induce Cholesterol Consumption Does Cell Wall Remodeling and Enables the Formation of Granuloma-Like Structures
Ana Cristina Doria dos Santos, Victor Hugo de Souza Marinho, Pedro Henrique de Aviz Silva, Barbarella de Matos Macchi, Mara Silvia Pinheiro Arruda, Edilene Oliveira da Silva, José Luiz Martins do Nascimento, Chubert Bernardo Castro de Sena
BioMed Research International.2019; 2019: 1. CrossRef
A highly selective fluorescent probe for real-time imaging of bacterial NAT2 and high-throughput screening of natural inhibitors for tuberculosis therapy
Yinzhu Jin, Zhenhao Tian, Xiangge Tian, Lei Feng, Jingnan Cui, Xiaochi Ma
Materials Chemistry Frontiers.2019; 3(1): 145. CrossRef
Cholesterol and fatty acids grease the wheels of Mycobacterium tuberculosis pathogenesis
Kaley M Wilburn, Rachael A Fieweger, Brian C VanderVen
Pathogens and Disease.2018;[Epub] CrossRef
Mce2R/Rv0586 of Mycobacterium tuberculosis is the functional homologue of FadR E. coli
Suhail Yousuf, Rajendra Kumar Angara, Ajit Roy, Shailesh Kumar Gupta, Rohan Misra, Akash Ranjan
Microbiology.2018; 164(9): 1133. CrossRef
Cell envelope stress in mycobacteria is regulated by the novel signal transduction ATPase IniR in response to trehalose
Maikel Boot, Vincent J. C. van Winden, Marion Sparrius, Robert van de Weerd, Alexander Speer, Roy Ummels, Tige Rustad, David R. Sherman, Wilbert Bitter, Carmen Buchrieser
PLOS Genetics.2017; 13(12): e1007131. CrossRef
“Genetic regulation of Mycobacterium tuberculosis in a lipid-rich environment”
Diana A. Aguilar-Ayala, Juan Carlos Palomino, Peter Vandamme, Anandi Martin, Jorge A. Gonzalez-y-Merchand
Infection, Genetics and Evolution.2017; 55: 392. CrossRef
MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis
Georgia L. Isom, Nathaniel J. Davies, Zhi-Soon Chong, Jack A. Bryant, Mohammed Jamshad, Maria Sharif, Adam F. Cunningham, Timothy J. Knowles, Shu-Sin Chng, Jeffrey A. Cole, Ian R. Henderson
Scientific Reports.2017;[Epub] CrossRef
Bacterial immunostat: Mycobacterium tuberculosis lipids and their role in the host immune response
Adriano Queiroz, Lee W. Riley
Revista da Sociedade Brasileira de Medicina Tropical.2017; 50(1): 9. CrossRef
The Sec Pathways and Exportomes of Mycobacterium tuberculosis
Brittany K. Miller, Katelyn E. Zulauf, Miriam Braunstein, William R. Jacobs Jr., Helen McShane, Valerie Mizrahi, Ian M. Orme
Microbiology Spectrum.2017;[Epub] CrossRef
Rv3723/LucA coordinates fatty acid and cholesterol uptake in Mycobacterium tuberculosis
Evgeniya V Nazarova, Christine R Montague, Thuy La, Kaley M Wilburn, Neelima Sukumar, Wonsik Lee, Shannon Caldwell, David G Russell, Brian C VanderVen
eLife.2017;[Epub] CrossRef
The transcriptome of Mycobacterium tuberculosis in a lipid-rich dormancy model through RNAseq analysis
Diana A. Aguilar-Ayala, Laurentijn Tilleman, Filip Van Nieuwerburgh, Dieter Deforce, Juan Carlos Palomino, Peter Vandamme, Jorge A. Gonzalez-Y-Merchand, Anandi Martin
Scientific Reports.2017;[Epub] CrossRef
Transcriptional Profiling of Mycobacterium tuberculosis Exposed toIn VitroLysosomal Stress
Wenwei Lin, Paola Florez de Sessions, Garrett Hor Keong Teoh, Ahmad Naim Nazri Mohamed, Yuan O. Zhu, Vanessa Hui Qi Koh, Michelle Lay Teng Ang, Peter C. Dedon, Martin Lloyd Hibberd, Sylvie Alonso, S. Ehrt
Infection and Immunity.2016; 84(9): 2505. CrossRef
Metabolic profile of Mycobacterium smegmatis reveals Mce4 proteins are relevant for cell wall lipid homeostasis
María Paz Santangelo, Adam Heuberger, Federico Blanco, Marina Forrellad, Catalina Taibo, Laura Klepp, Julia Sabio García, Pablo I. Nikel, Mary Jackson, Fabiana Bigi
Metabolomics.2016;[Epub] CrossRef
An orphaned Mce‐associated membrane protein of Mycobacterium tuberculosis is a virulence factor that stabilizes Mce transporters
Ellen Foot Perkowski, Brittany K. Miller, Jessica R. McCann, Jonathan Tabb Sullivan, Seidu Malik, Irving Coy Allen, Virginia Godfrey, Jennifer D. Hayden, Miriam Braunstein
Molecular Microbiology.2016; 100(1): 90. CrossRef
Standardization of natural mycolic acid antigen composition and production for use in biomarker antibody detection to diagnose active tuberculosis
F.L. Ndlandla, V. Ejoh, A.C. Stoltz, B. Naicker, A.D. Cromarty, S. van Wyngaardt, M. Khati, L.S. Rotherham, Y. Lemmer, J. Niebuhr, C.R. Baumeister, J.R. Al Dulayymi, H. Swai, M.S. Baird, J.A. Verschoor
Journal of Immunological Methods.2016; 435: 50. CrossRef
Screening of the antimycobacterial activity of novel lipophilic agents by the modified broth based method
Mehdi Zandhaghighi, Kiarash Ghazvini, Zahra Meshkat, Seyed Abdolrahim Rezaee, Mohammad Derakhshan, Saman Soleimanpour, Farzin Hadizadeh
Journal of Clinical Tuberculosis and Other Mycobacterial Diseases.2016; 3: 1. CrossRef
Comparative metabolic profiling ofmce1operon mutant vs wild-typeMycobacterium tuberculosisstrains
Adriano Queiroz, Daniel Medina-Cleghorn, Olivera Marjanovic, Daniel K. Nomura, Lee W. Riley, Patricia Bozza
Pathogens and Disease.2015; 73(8): ftv066. CrossRef
Mycobacterium tuberculosis effectors involved in host–pathogen interaction revealed by a multiple scales integrative pipeline
Wu Li, Xiangyu Fan, Quanxin Long, Longxiang Xie, Jianping Xie
Infection, Genetics and Evolution.2015; 32: 1. CrossRef
Label-free Quantitative Proteomics Reveals a Role for the Mycobacterium tuberculosis SecA2 Pathway in Exporting Solute Binding Proteins and Mce Transporters to the Cell Wall*
Meghan E. Feltcher, Harsha P. Gunawardena, Katelyn E. Zulauf, Seidu Malik, Jennifer E. Griffin, Christopher M. Sassetti, Xian Chen, Miriam Braunstein
Molecular & Cellular Proteomics.2015; 14(6): 1501. CrossRef
Role of host- and pathogen-associated lipids in directing the immune response in mycobacterial infections, with emphasis onMycobacterium aviumsubsp.paratuberculosis
Shyamala Thirunavukkarasu, Kumudika de Silva, Karren M. Plain, Richard J. Whittington
Critical Reviews in Microbiology.2014; : 1. CrossRef
Inhibition of toll-like receptor 2 (TLR-2)-mediated response in human alveolar epithelial cells by mycolic acids andMycobacterium tuberculosismce1operon mutant
Patricia C. Sequeira, Ryan H. Senaratne, Lee W. Riley
Pathogens and Disease.2014; 70(2): 132. CrossRef

Research Support, Non-U.S. Gov't

Low-Scale Expression and Purification of an Active Putative Iduronate 2-Sulfate Sulfatase-Like Enzyme from Escherichia coli K12: Edwin David Morales-Álvarez , Claudia Marcela Rivera-Hoyos , Angélica María Baena-Moncada , Patricia Landázuri , Raúl A. Poutou-Piñales , Homero Sáenz-Suárez , Luis A. Barrera , Olga Y. Echeverri-Peña; J. Microbiol. 2013;51(2):213-221. Published online April 27, 2013; DOI: https://doi.org/10.1007/s12275-013-2416-2

44 View
0 Download
15 Scopus

Abstract: The sulfatase family involves a group of enzymes with a large degree of similarity. Until now, sixteen human sulfatases have been identified, most of them found in lysosomes. Human deficiency of sulfatases generates various genetic disorders characterized by abnormal accumulation of sulfated intermediate compounds. Mucopolysaccharidosis type II is characterized by the deficiency of iduronate 2-sulfate sulfatase (IDS), causing the lysosomal accumulation of heparan and dermatan sulfates. Currently, there are several cases of genetic diseases treated with enzyme replacement therapy, which have generated a great interest in the development of systems for recombinant protein expression. In this work we expressed the human recombinant IDS-Like enzyme (hrIDS-Like) in Escherichia coli DH5α. The enzyme concentration revealed by ELISA varied from 78.13 to 94.35 ng/ml and the specific activity varied from 34.20 to 25.97 nmol/h/mg. Western blotting done after affinity chromatography purification showed a single band of approximately 40 kDa, which was recognized by an IgY polyclonal antibody that was developed against the specific peptide of the native protein. Our 100 ml-shake-flask assays allowed us to improve the enzyme activity seven fold, compared to the E. coli JM109/pUC13-hrIDS-Like system. Additionally, the results obtained in the present study were equal to those obtained with the Pichia pastoris GS1115/ pPIC-9-hrIDS-Like system (3 L bioreactor scale). The system used in this work (E. coli DH5α/pGEX-3X-hrIDS-Like) emerges as a strategy for improving protein expression and purification, aimed at recombinant protein chemical characterization, future laboratory assays for enzyme replacement therapy, and as new evidence of active putative sulfatase production in E. coli.

Research Support, N.I.H., Extramural

Sulfolipid Accumulation in Mycobacterium tuberculosis Disrupted in the mce2 Operon: Olivera Marjanovic , Anthony T. Iavarone , Lee W. Riley; J. Microbiol. 2011;49(3):441-447. Published online June 30, 2011; DOI: https://doi.org/10.1007/s12275-011-0435-4

35 View
0 Download
23 Scopus

Abstract: Mycobacterium tuberculosis, the causative agent of tuberculosis, has a lipid-rich cell wall that serves as an effective barrier against drugs and toxic host cell products, which may contribute to the organism’s persistence in a host. M. tuberculosis contains four homologous operons called mce (mce1-4) that encode putative ABC transporters involved in lipid importation across the cell wall. Here, we analyzed the lipid composition of M. tuberculosis disrupted in the mce2 operon. High resolution mass spectrometric and thin layer chromatographic analyses of the mutant’s cell wall lipid extracts showed accumulation of SL-1 and SL1278 molecules. Radiographic quantitative analysis and densitometry revealed 2.9, 3.9 and 9.8-fold greater amount of [35S] SL-1 in the mce2 operon mutant compared to the wild type M. tuberculosis during the early/mid logarithmic, late logarithmic and stationary phase of growth in liquid broth, respectively. The amount of [35S] SL1278 in the mutant also increased progressively over the same growth phases. The expression of the mce2 operon genes in the wild type strain progressively increased from the logarithmic to the stationary phase of bacterial growth in vitro, which inversely correlated with the proportion of radiolabel incorporation into SL-1 and SL1278 at these phases. Since the mce2 operon is regulated in wild type M. tuberculosis, its cell wall may undergo changes in SL-1 and SL1278 contents during a natural course of infection and this may serve as an important adaptive strategy for M. tuberculosis to maintain persistence in a host.

Research Support, Non-U.S. Gov'ts

Bacillus megaterium Strain XTBG34 Promotes Plant Growth by Producing 2-Pentylfuran: Changsong Zou , Zhifang Li , Diqiu Yu; J. Microbiol. 2010;48(4):460-466. Published online August 20, 2010; DOI: https://doi.org/10.1007/s12275-010-0068-z

40 View
0 Download
147 Scopus

Abstract: Several chemical changes in soil are associated with plant growth-promoting rhizobacteria. An endosporeforming bacterium, strain XTBG34, was isolated from a Xishuangbanna Tropical Botanical Garden soil sample and identified as Bacillus megaterium. The strain’s volatiles had remarkable plant growth promotion activity in Arabidopsis thaliana plants; after 15 days treatment, the fresh weight of plants inoculated with XTBG34 was almost 2-fold compared with those inoculated with DH5α. Head space volatile compounds produced by XTBG34, trapped with headspace solid phase microextraction and identified by gas chromatography–mass spectrometry, included aldehydes, alkanes, ketones and aroma components. Of the 11 compounds assayed for plant growth promotion activity in divided Petri plates, only 2-pentylfuran increased plant growth. We have therefore identified a new plant growth promotion volatile of B. megaterium XTBG34, which deserves further study in the mechanisms of interaction between plant growth-promoting rhizobacteria and plants.

Patterns of Survival and Volatile Metabolites of Selected Lactobacillus Strains During Long-Term Incubation in Milk: Łucja Łaniewska-Trokenheim , Magdalena Olszewska , Marta Mik&# , Anna Zadernowska; J. Microbiol. 2010;48(4):445-451. Published online August 20, 2010; DOI: https://doi.org/10.1007/s12275-010-0056-3

46 View
0 Download
12 Scopus

Abstract: The focus of this study was to monitor the survival of populations and the volatile compound profiles of selected Lactobacillus strains during long-term incubation in milk. The enumeration of cells was determined by both the Direct Epifluorescent Filter Technique using carboxyfluorescein diacetate (CFDA) staining and the plate method. Volatile compounds were analysed by the gas-chromatography technique. All strains exhibited good survival in cultured milks, but Lactobacillus crispatus L800 was the only strain with comparable growth and viability in milk, assessed by plate and epifluorescence methods. The significant differences in cell numbers between plate and microscopic counts were obtained for L. acidophilus strains. The investigated strains exhibited different metabolic profiles. Depending on the strain used, 3 to 8 compounds were produced. The strains produced significantly higher concentrations of acetic acid, compared to other volatiles. Lactobacillus strains differed from one another in number and contents of the volatile compounds.

Purification of Filamentous Bacteriophage M13 by Expanded Bed Anion Exchange Chromatography: Tau Chuan Ling , Chee Kin Loong , Wen Siang Tan , Beng Ti Tey , Wan Mohammad Wan Abdullah , Arbakariya Ariff; J. Microbiol. 2004;42(3):228-232.; DOI: https://doi.org/2084 [pii]

47 View
0 Download

Abstract: In this paper, we investigated the development of a simplified and rapid primary capture step for the recovery of M13 bacteriophage from particulate-containing feedstock. M13 bacteriophage, carrying an insert, was propagated and subsequently purified by the application of both conventional multiple steps and expanded bed anion exchange chromatography. In the conventional method, precipitation was conducted with PEG/NaCl, and centrifugation was also performed. In the single step expanded bed anion exchange adsorption, UpFront FastLine^TM 20 (20 mm i.d.) from UpFront Chromatography was used as the contactor, while 54 ml (H_o=15cm) of STREAMLINE DEAE (r=1.2 g/cm^3) from Amersham Pharmacia Biotechnology was used as the anion exchanger. The performance of the two methods were evaluated, analysed, and compared. It was demonstrated that the purification of the M13 bacteriophage, using expanded bed anion exchange adsorption, yielded the higher recovery percentage, at 82.86%. The conventional multiple step method yielded the lower recovery percentage, 36.07%. The generic application of this integrated technique has also been assessed.

Expression and Purification of a Recombinant scFv towards the Exotoxin of the Pathogen, Burkholderia pseudomallei: Kue-Peng Lim , HongBin Li , Sheila Nathan; J. Microbiol. 2004;42(2):126-132.; DOI: https://doi.org/2034 [pii]

42 View
0 Download

Abstract: A single chain variable fragment (scFv) specific towards B. pseudomallei exotoxin had previously been generated from an existing hybridoma cell line (6E6AF83B) and cloned into the phage display vector pComb3H. In this study, the scFv was subcloned into the pComb3X vector to facilitate the detection and purification of expressed antibodies. Detection was facilitated by the presence of a hemagglutinin (HA) tag, and purification was facilitated by the presence of a histidine tag. The culture was grown at 30^oC until log phase was achieved and then induced with 1 mM IPTG in the absence of any additional carbon source. Induction was continued at 30^oC for five h. The scFv was discerned by dual processes-direct enzyme-linked immunosorbent assays (ELISA), and Western blotting. When compared to E. coli strains ER2537 and HB2151, scFv expression was observed to be highest in the E. coli strain Top10F'. The expressed scFv protein was purified via nickel-mediated affinity chromatography and results indicated that two proteins a 52 kDa protein, and a 30 kDa protein were co-purified. These antibodies, when blotted against immobilized exotoxin, exhibited significant specificity towards the exotoxin, compared to other B. pseudomallei antigens. Thus, these antibodies should serve as suitable reagents for future affinity purification of the exotoxin.

Lipoppolysaccharide yields form Rhodobacter capasulatus with indirect ELISA: Yoo, Tae Eun , Lee, Hyun Soon; J. Microbiol. 1996;34(3):255-262.

42 View
0 Download

Abstract: The lipopolysaccharide (LPS) yields were measured in Rhodobacter capsulatus under several conditions by the ELISA method. The purification of LPS was done by affinity chromatography of IgG coupled CNBr-activated sepharose-4B instead of ultra-centrifugation. The purity of the LPS didn't show much difference between affinity chromatography and ultra-centrifugation method, but affinity chromatography method required much fewer organisms and was more convenient. LPS yield was measured in ng units by the ELISA method. Mannitol was a better single carbon source than other sugars, but mixing two carbon sources resulted in greater LPS yields than any sugar alone. LPS yield was directly proportional to NH₄CI concentration, with optimum yields at 0.05% nitrogen. In contrest to LPS yields, which decreased at 0.005% nitrogen concentration total protein was increased 16 times. Calcium influenced LPS yields. At 0.7 mM CaCI₂, the LPS yield was 16.5 ㎍/mg DW, five times the yield without calcium.

Fungal-sporulation suppressing substances produced by pseudomonas aeruginosa KMCS-1: Min, Bu Yong , Shim, Jae Young , Kim, Kun Woo , Lee, Jong Kyu , Yoon, Kwon Sang; J. Microbiol. 1996;34(3):284-288.

43 View
0 Download

Abstract: Among the bacteria isolated form compost piles of cattle excretion in a pasture located at the suburbs of Chunchon city, Pseudomonas aeruginosa KMCS-1 was selected for the test of antifungal substances produced. Six fractions were separated by silica gel column chromatography, and then the antifungal activity of each fraction was assayed against Escherichia coli, Bacillus subtilis, Candida albicans, Rhizopus sp., Aspergillus nidulans, Coprinus cinereus, and Pyricularia oryzae by paper disc method. Two fractions showed significant suppressive activities against A. nidulans, C. cinereus, and P. oryzae; however, their mycelial growth was not affected by neither of these fractions. Inhibitory activities of these fractions to sporulation was assayed at the concentration of 50. 25, 12. 5, and 6.25 ㎍/ml and the average inhibition rates against sporulation of A. nidulans, C. cinereus, and P. oryzae were 94.0, 98.3, and 77.9%, respectively. Further purification and analysis of active substances are now being conducted.

First
Prev
Page of 1
Next
Last

Search