Journal of Microbiology

Research Support, Non-U.S. Gov't

Synergistic Effects of the Combination of Galangin with Gentamicin against Methicillin-Resistant Staphylococcus aureus: Young-Seob Lee , Ok-Hwa Kang , Jang-Gi Choi , You-Chang Oh , Hee-Sung Chae , Jong Hak Kim , Hyun Park , Dong Hwan Sohn , Zheng-Tao Wang , Dong-Yeul Kwon; J. Microbiol. 2008;46(3):283-288. Published online July 5, 2008; DOI: https://doi.org/10.1007/s12275-008-0012-7

Abstract: The antimicrobial killing activity toward methicillin-resistant Staphylococcus aureus (MRSA) has been a serious emerging global issue. New effective antimicrobials and/or new approaches to settle this issue are urgently needed. The oriental herb, Alpinia officinarum, has been used in Korea for several hundreds of years to treat various infectious diseases. As it is well known, one of the active constituents of Alpinia officinarum is galangin. Against the 17 strains, the minimum inhibitory concentrations (MICs) of galangin (GAL) were in the range of 62.5~125 μg/ml, and the MICs of gentamicin (GEN) ranged from 1.9 μg/ml to 2,000 μg/ml. The fractional inhibitory concentrations (FICs) of GAL, in combination with GEN, against 3 test strains were 0.4, 3.9, and 250 μg/ml, and were all 15.62 μg/ml in GEN. The FIC index showed marked synergism in the value range of 0.19 to 0.25. By determining time-kill curves, also confirmed the low synergism of the GAL and GEN combination against 4 h, 8 h, 12 h, and 24 h cultured MRSA. The time-kill study results indicated a low synergistic effect against 3 test strains. Thus, the mixture of GAL and GEN could lead to the development of new combination antibiotics against MRSA infection.

Journal Article

In vitro Activity of Kaempferol Isolated from the Impatiens balsamina alone and in Combination with Erythromycin or Clindamycin against Propionibacterium acnes: Young-Hee Lim , In-Hwan Kim , Jung-Ju Seo; J. Microbiol. 2007;45(5):473-477.; DOI: https://doi.org/2587 [pii]

Abstract: The in vitro antibacterial activity against antibiotic-resistant Propionibacterium acnes of kaempferol isolated from the Impatiens balsamina alone and in combination with erythromycin or clindamycin antibiotics was investigated. The antibiotic combination effect against antibiotic-resistant P. acnes was studied by checkerboard test. Kaempferol and quercetin demonstrated antibacterial activities against P. acnes. Minimum inhibitory concentrations (MICs) for both compounds were ≤32 μg/ml and ≤64 μg/ml for clindamycin-sensitive and-resistant P. acnes, respectively. The four combination formulations (kaempferol and either erythromycin or clindamycin; quercetin and either erythromycin or clindamycin) exhibited a synergic inhibition of P. acnes growth. The combination of kaempferol with quercetin showed an indifferent effect. The combination of clindamycin with kaempferol or quercetin showed a greater synergic effect than that of erythromycin with kaempferol or quercetin. Thus, these combinations demonstrated the potential to treat acne.

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