Hymenochaetales Oberw. is an order classified in Basidiomycota of Fungi, and species in this order display notable diversity. They exhibit various fruiting body shapes, including clavarioid, effused-reflexed, and resupinate basidiomes.
Few mycorrhizal species have been reported in Hymenochaetales, but wood-decaying species dominate the order. Hymenochaetaceae Imazeki & Toki and Schizoporaceae Jülich are the most species-rich families within Hymenochaetales, and most species in the Republic of Korea belong to these two families. As such, current taxonomic classification and nomenclature are not reflected upon species in the remaining Hymenochaetales families. For this study, a multifaceted morphological and multigenetic marker-based phylogenetic investigation was conducted to, firstly, comprehensively identify understudied Hymenochaetales specimens in Korea and, secondly, reflect the updates on the species classification. Five genetic markers were assessed for the phylogenetic analysis: nuclear small subunit ribosomal DNA (nSSU), internal transcribed spacer (ITS), nuclear large subunit ribosomal DNA (nLSU), RNA polymerase II subunit 2 gene (RPB2), and translation elongation factor 1 gene (TEF1). The results from phylogenetic analysis supported 18 species classified under eight families (excluding Hymenochaetaceae and Schizoporaceae) in Korea. Species formerly placed in Rickenellaceae and Trichaptum sensu lato have been systematically revised based on recent taxonomic reconstructions. In addition, our findings revealed one new species, Rickenella umbelliformis, and identified five formerly nationally unreported species classified under five understudied families. Our findings contribute to a better understanding of Hymenochaetales diversity and highlight the need for continued research.
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Molecular phylogeny and morphology reveal four new species in Hymenochaetales and one new species in Cantharellales from Southwestern China Jianling Zhang, Zirui Gu, Chunqin Zhou, Hongmin Zhou MycoKeys.2025; 115: 87. CrossRef
Pseudomonas aeruginosa, an opportunistic human pathogen,
causes many biofilm-mediated chronic infections. In this study,
biofilm structures of various clinical strains of P. aeruginosa
isolated from hospitalized patients were examined and their
influence on the biofilm-dispersing effects of chemicals was
investigated. The clinical isolates formed structurally distinct
biofilms that could be classified into three different groups:
1) mushroom-like, 2) thin flat, and 3) thick flat structures.
A dispersion of these differently structured biofilms was induced
using two biofilm-dispersing agents, anthranilate and
sodium nitroprusside (SNP). Although both SNP and anthranilate
could disperse all types of biofilms, the thick flat biofilms
were dispersed less efficiently than the biofilms of other
structures. This suggests that biofilm-dispersing agents have
higher potency on the biofilms of porous structures than on
densely packed biofilms.
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Thermoregulation of
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Biofilm Formation
Suran Kim, Xi-Hui Li, Hyeon-Ji Hwang, Joon-Hee Lee, Danilo Ercolini Applied and Environmental Microbiology.2020;[Epub] CrossRef
In recent decades, many researchers have written numerous
articles about microbial biofilms. Biofilm is a complex community
of microorganisms and an example of bacterial group
behavior. Biofilm is usually considered a sessile mode of life
derived from the attached growth of microbes to surfaces, and
most biofilms are embedded in self-produced extracellular
matrix composed of extracellular polymeric substances (EPSs),
such as polysaccharides, extracellular DNAs (eDNA), and
proteins. Dispersal, a mode of biofilm detachment indicates
active mechanisms that cause individual cells to separate from
the biofilm and return to planktonic life. Since biofilm cells
are cemented and surrounded by EPSs, dispersal is not simple
to do and many researchers are now paying more attention
to this active detachment process. Unlike other modes
of biofilm detachment such as erosion or sloughing, which
are generally considered passive processes, dispersal occurs
as a result of complex spatial differentiation and molecular
events in biofilm cells in response to various environmental
cues, and there are many biological reasons that force bacterial
cells to disperse from the biofilms. In this review, we
mainly focus on the spatial differentiation of biofilm that is
a prerequisite for dispersal, as well as environmental cues
and molecular events related to the biofilm dispersal. More
specifically, we discuss the dispersal-related phenomena and
mechanisms observed in Pseudomonas aeruginosa, an important
opportunistic human pathogen and representative
model organism for biofilm study.
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