Clostridium difficile infection (CDI) is one of the most common
nosocomial infections. Dysbiosis of the gut microbiota
due to consumption of antibiotics is a major contributor to
CDI. Recently, fecal microbiota transplantation (FMT) has
been applied to treat CDI. However, FMT has important limitations
including uncontrolled exposure to pathogens and
standardization issues. Therefore, it is necessary to evaluate
alternative treatment methods, such as bacteriotherapy, as
well as the mechanism through which beneficial bacteria inhibit
the growth of C. difficile. Here, we report bile acid-mediated
inhibition of C. difficile by Bacteroides strains which
can produce bile salt hydrolase (BSH). Bacteroides strains
are not commonly used to treat CDI; however, as they comprise
a large proportion of the intestinal microbiota, they can
contribute to bile acid-mediated inhibition of C. difficile. The
inhibitory effect on C. difficile growth increased with increasing
bile acid concentration in the presence of Bacteroides
ovatus SNUG 40239. Furthermore, this inhibitory effect on
C. difficile growth was significantly attenuated when bile acid
availability was reduced by cholestyramine, a bile acid sequestrant.
The findings of this study are important due to
the discovery of a new bacterial strain that in the presence
of available bile acids inhibits growth of C. difficile. These results will facilitate development of novel bacteriotherapy
strategies to control CDI.
Citations
Citations to this article as recorded by
New insights into microbial bile salt hydrolases: from physiological roles to potential applications Zixing Dong, Shuangshuang Yang, Cunduo Tang, Dandan Li, Yunchao Kan, Lunguang Yao Frontiers in Microbiology.2025;[Epub] CrossRef
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