Journal of Microbiology

Journal Articles

Composition and abundance of microbiota in the pharynx in patients with laryngeal carcinoma and vocal cord polyps: Hongli Gong , Boyan Wang , Yi Shi , Yong Shi , Xiyan Xiao , Pengyu Cao , Lei Tao , Yuezhu Wang , Liang Zhou; J. Microbiol. 2017;55(8):648-654. Published online July 28, 2017; DOI: https://doi.org/10.1007/s12275-017-6636-8

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The pharynx is an important site of microbiota colonization, but the bacterial populations at this site have been relatively unexplored by culture-independent approaches. The aim of this study was to characterize the microbiota structure of the pharynx. Pyrosequencing of 16S rRNA gene libraries was used to characterize the pharyngeal microbiota using swab samples from 68 subjects with laryngeal cancer and 28 subjects with vocal cord polyps. Overall, the major phylum was Firmicutes, with Streptococcus as the predominant genus in the pharyngeal communities. Nine core operational taxonomic units detected from Streptococcus, Fusobacterium, Prevotella, Granulicatella, and Veillonella accounted for 21.3% of the total sequences detected. However, there was no difference in bacterial communities in the pharynx from patients with laryngeal cancer and vocal cord polyps. The relative abundance of Firmicutes was inversely correlated with Fusobacteria, Proteobacteria, Actinobacteria, and Bacteroidetes. The correlation was evident at the genus level, and the relative abundance of Streptococcus was inversely associated with Fusobacterium, Leptotrichia, Neisseria, Actinomyces, and Prevotella. This study presented a profile for the overall structure of the microbiota in pharyngeal swab samples. Inverse correlations were found between Streptococcus and other bacterial communities, suggesting that potential antagonism may exist among pharyngeal microbiota.

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Effects of squamous cell carcinoma and smoking status on oropharyngeal and laryngeal microbial communities
Maximilian Oberste, Brit Elisabeth Böse, Luis Gustavo dos Anjos Borges, Howard Junca, Iris Plumeier, Silke Kahl, Frank Simon, Achim Georg Beule, Claudia Rudack, Dietmar H. Pieper
Head & Neck.2024; 46(1): 145. CrossRef
Comparison of the bacterial microbiome in the pharynx and nasal cavity of persistent, intermittent carriers and non-carriers of Staphylococcus aureus
Samuel González-García, Aida Hamdan-Partida, Julia Pérez-Ramos, José Félix Aguirre-Garrido, Anaíd Bustos-Hamdan, Jaime Bustos-Martínez
Journal of Medical Microbiology .2024;[Epub] CrossRef
Fusobacterium nucleatum impairs DNA mismatch repair and stability in patients with squamous cell carcinoma of the head and neck
Chi‐Yao Hsueh, Hui‐Ching Lau, Qiang Huang, Hongli Gong, Ji Sun, Pengyu Cao, Chunyan Hu, Ming Zhang, Lei Tao, Liang Zhou
Cancer.2022; 128(17): 3170. CrossRef
Raman fl uorescence technologies for early detection of infl ammatory and oncological disorders as a part of public health strategy and a tool for improving the quality of medical care: a review
A. B. Timurzieva
Laser Medicine.2022; 25(4): 42. CrossRef
Cross-comparison of microbiota in the oropharynx, hypopharyngeal squamous cell carcinoma and their adjacent tissues through quantitative microbiome profiling
Hui-Ching Lau, Yujie Shen, Huiying Huang, Xiaohui Yuan, Mengyou Ji, Hongli Gong, Chi-Yao Hsueh, Liang Zhou
Journal of Oral Microbiology.2022;[Epub] CrossRef
Oral microbiota in oropharyngeal cancers: Friend or foe?
Riccardo Nocini, Lorenzo Lo Muzio, Davide Gibellini, Giovanni Malerba, Michele Milella, Salvatore Chirumbolo, Nicoletta Zerman
Frontiers in Oncology.2022;[Epub] CrossRef
Oropharynx microbiota transitions in hypopharyngeal carcinoma treatment of induced chemotherapy followed by surgery
Hui-Ching Lau, Chi-Yao Hsueh, Hongli Gong, Ji Sun, Hui-Ying Huang, Ming Zhang, Liang Zhou
BMC Microbiology.2021;[Epub] CrossRef
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BioMed Research International.2021; 2021: 1. CrossRef
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Frontiers in Microbiology.2020;[Epub] CrossRef
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Chi-Yao Hsueh, Hongli Gong, Ning Cong, Ji Sun, Hui-Ching Lau, Yang Guo, Qiang Huang, Xiaohui Yuan, Ming Zhang, Lei Tao, Liang Zhou, Harold L. Drake
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Potential role of microbiome in oncogenesis, outcome prediction and therapeutic targeting for head and neck cancer
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Oral Oncology.2019; 99: 104453. CrossRef

Detecting Nonculturable Bacteria in the Active Mycorrhizal Zone of the Pine Mushroom Tricholoma matsutake: Ryota Kataoka , Zaki Anwar Siddiqui , Junichi Kikuchi , Masaki Ando , Rina Sriwati , Ai Nozaki , Kazuyoshi Futai; J. Microbiol. 2012;50(2):199-206. Published online April 27, 2012; DOI: https://doi.org/10.1007/s12275-012-1371-7

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Abstract: The fungus Tricholoma matsutake forms an ectomycorrhizal relationship with pine trees. Its sporocarps often develop in a circle, which is commonly known as a fairy ring. The fungus produces a solid, compact, white aggregate of mycelia and mycorrhizae beneath the fairy ring, which in Japanese is called a ‘shiro’. In the present study, we used soil dilution plating and molecular techniques to analyze the bacterial communities within, beneath, and outside the T. matsutake fairy ring. Soil dilution plating confirmed previous reports that bacteria and actinomycetes are seldom present in the soil of the active mycorrhizal zone of the T. matsutake shiro. In addition, the results showed that the absence of bacteria was strongly correlated with the presence of T. matsutake mycorrhizae. The results demonstrate that bacteria, especially aerobic and heterotrophic forms, and actinomycetes, are strongly inhibited by T. matsutake. Indeed, neither bacteria nor actinomycetes were detected in 11.3% of 213 soil samples from the entire shiro area by culture-dependent
methods
. However, molecular techniques demonstrated that some bacteria, such as individual genera of Sphingomonas and Acidobacterium, were present in the active mycorrhizal zone, even though they were not detected in soil assays using the dilution plating technique.

Research Support, Non-U.S. Gov't

Analyses of Bacterial Communities in Meju, a Korean Traditional Fermented Soybean Bricks, by Cultivation-Based and Pyrosequencing Methods: Yi-Seul Kim , Min-Cheol Kim , Soon-Wo Kwon , Soo-Jin Kim , In-Cheol Park , Jong-Ok Ka , Hang-Yeon Weon; J. Microbiol. 2011;49(3):340-348. Published online June 30, 2011; DOI: https://doi.org/10.1007/s12275-011-0302-3

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Abstract: Despite the importance of meju as a raw material used to make Korean soy sauce (ganjang) and soybean paste (doenjang), little is known about the bacterial diversity of Korean meju. In this study, the bacterial communities in meju were examined using both culture-dependent and independent methods in order to evaluate the diversity of the bacterial population. Analyses of the 16S rRNA gene sequences of the bacterial strains isolated from meju samples showed that the dominant species were related to members of the genera Bacillus, Enterococcus, and Pediococcus. The community DNAs extracted from nine different meju samples were analyzed by barcoded pyrosequencing method targeting of the V1 to V3 hypervariable regions of the 16S rRNA gene. In total, 132,374 sequences, with an average read length of 468 bp, were assigned to several phyla, with Firmicutes (93.6%) representing the predominant phylum, followed by Proteobacteria (4.5%) and Bacteroidetes (0.8%). Other phyla accounted for less than 1% of the total bacterial sequences. Most of the Firmicutes were Bacillus and lactic acid bacteria, mainly represented by members of the genera Enterococcus, Lactococcus, and Leuconostoc, whose ratio varied among different samples. In conclusion, this study indicated that the bacterial communities in meju were very diverse and a complex microbial consortium containing various microorganisms got involved in meju fermentation than we expected before.

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