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Research Support, Non-U.S. Gov't
- Therapeutic potential of an AcHERV-HPV L1 DNA vaccine
- Hee-Jung Lee , Jong Kwang Yoon , Yoonki Heo , Hansam Cho , Yeondong Cho , Yongdae Gwon , Kang Chang Kim , Jiwon Choi , Jae Sung Lee , Yu-Kyoung Oh , Young Bong Kim
- J. Microbiol. 2015;53(6):415-420. Published online May 30, 2015
- DOI: https://doi.org/10.1007/s12275-015-5150-0
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Abstract
- Cervical cancer is strongly associated with chronic human papillomavirus infections, among which HPV16 is the most common. Two commercial HPV vaccines, Gardasil and Cervarix are effective for preventing HPV infection, but cannot be used to treat existing HPV infections. Previously, we developed a human endogenous retrovirus (HERV)-enveloped recombinant baculovirus capable of delivering the L1 genes of HPV types 16, 18, and 58 (AcHERV-HP16/18/58L1, AcHERV-HPV). Intramuscular administration of AcHERVHPV vaccines induced a strong cellular immune response as well as a humoral immune response. In this study, to examine the therapeutic effect of AcHERV-HPV in a mouse model, we established an HPV16 L1 expressing tumor cell line. Compared to Cervarix, immunization with AcHERVHPV greatly enhanced HPV16 L1-specific cytotoxic T lymphocytes (CTL) in C57BL/6 mice. Although vaccination could not remove preexisting tumors, strong CTL activity retarded the growth of inoculated tumor cells. These results indicate that AcHERV-HPV could serve as a potential therapeutic DNA vaccine against concurrent infection with HPV 16, 18, and 58.
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Citations
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- Current Updates on Cancer-Causing Types of Human Papillomaviruses (HPVs) in East, Southeast, and South Asia
Chichao Xia, Sile Li, Teng Long, Zigui Chen, Paul K. S. Chan, Siaw Shi Boon
Cancers.2021; 13(11): 2691. CrossRef - In Silico Design and Immunological Studies of Two Novel Multiepitope DNA-Based Vaccine Candidates Against High-Risk Human Papillomaviruses
Matin Kayyal, Azam Bolhassani, Zahra Noormohammadi, Majid Sadeghizadeh
Molecular Biotechnology.2021; 63(12): 1192. CrossRef - Unraveling the Genome-Wide Impact of Recombinant Baculovirus Infection in Mammalian Cells for Gene Delivery
Ha Youn Shin, Hanul Choi, Nahyun Kim, Nayoung Park, Heesun Kim, Jaebum Kim, Young Bong Kim
Genes.2020; 11(11): 1306. CrossRef - Treatment with Radix Euphorbiae Ebracteolatae Significantly Decreases the Expression of E6 and L1, and Increases the Expression of p53 and Rb in HPV18-infected Human Foreskin Keratinocytes
Xiang He, Xufeng He, Ping Xu, Lili Yang, Xin Ma, Wen Li, Huimin Zhang
Current Molecular Medicine.2019; 19(1): 20. CrossRef - Trial watch: DNA-based vaccines for oncological indications
Stefano Pierini, Renzo Perales-Linares, Mireia Uribe-Herranz, Jonathan G. Pol, Laurence Zitvogel, Guido Kroemer, Andrea Facciabene, Lorenzo Galluzzi
OncoImmunology.2017; 6(12): e1398878. CrossRef - Broad-spectrum antiviral properties of andrographolide
Swati Gupta, K. P. Mishra, Lilly Ganju
Archives of Virology.2017; 162(3): 611. CrossRef
- Current Updates on Cancer-Causing Types of Human Papillomaviruses (HPVs) in East, Southeast, and South Asia
- Characterization and Cytotoxic Activities of Nonadecanoic Acid Produced by Streptomyces scabiei subsp. chosunensis M0137 (KCTC 9927)
- Jin-Cheol Yoo , Ji-Man Han , Seung-Kwan Nam , Ok-Hyun Ko , Cheol-Hee Choi , Keun-Hong Kee , Jae-Kyung Sohng , Jung-Sun Jo , Chi-Nam Seong
- J. Microbiol. 2002;40(4):331-334.
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Abstract
- The substance 0116p, which exhibits cytotoxicity against human macrophage cell line THP-1, was isolated from a mycelial extract of Streptomyces scabiei subsp. chosunensis M0137. The cytotoxic substance was purified by Diaion-HP20 adsorption, solvent extraction, Sephadex LH-20 column chromatography, and silica-gel column chromatography. The molecular formula is C_19 H_38 O_2 (MW 301.10) based on elemental and spectrometric analysis. It was identified as nonadecanoic acid by NMR spectral data. It exhibits cytotoxic activities in various human cancer cell lines, including A549, SK-OV-3, SK-MEL-2 and HCT-15. In addition, 0116p also inhibits IL-12 production in lipopolysaccharide-activated macrophages.
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