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- Conditional probability analysis of multidrug resistance in Gram-negative bacilli isolated from tertiary medical institutions in South Korea during 1999–2009
- Yong-Hak Kim
- J. Microbiol. 2016;54(1):50-56. Published online January 5, 2016
- DOI: https://doi.org/10.1007/s12275-016-5579-9
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- 6 Crossref
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Abstract
- Multidrug resistance of Gram-negative bacilli is a major problem globally. However, little is known about the combined probability of resistance to various antibiotics. In this study, minimum inhibitory concentrations of widely used antibiotics were determined using clinical isolates of Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Acinetobacter baumannii, randomly chosen from strain collections created during 1999–2009 in tertiary medical institutions in Seoul, South Korea. To analyze combined efficacy of antibiotics against a subgroup of isolates, conditional probabilities were determined based on arbitrary, non-independent patterns of antimicrobial susceptibility and resistance. Multidrug resistance, defined as resistance to three or more classes of antibiotics, was observed in the following order: A. baumannii (96%), P. aeruginosa (65%), E. coli (52%), and K. pneumoniae (7%). A. baumannii strains resistant to gentamicin were found to be resistant to a number of antibiotics, except for colistin and polymyxin B. Resistance to gentamicin following exposure to this antibiotic was highly likely to lead to multidrug resistance in all four microbes. This study shows a causal relationship between gentamicin resistance and the prevalence of multidrug resistance in clinical isolates of Gramnegative bacilli in South Korea during 1999–2009 and suggests the importance of prudent use of gentamicin in hospitals.
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- Analysis of Predictive Factors of Antibiotic Resistant Pseudomonas aeruginosa in Children with Acute Appendicitis
Mengqi Wan, Mengjie Yu, Juhua Xiao Jiangxi, Qiang Chen, Nuoya Li, Jun Lei, Weilong Chen, Shouhua Zhang, Qiang Tao
Iranian Journal of Pediatrics.2021;[Epub] CrossRef - Bacterial culture and antibiotic susceptibility in patients with acute appendicitis
Dae Woon Song, Byung Kwan Park, Suk Won Suh, Seung Eun Lee, Jong Won Kim, Joong-Min Park, Hye Ryoun Kim, Mi-Kyung Lee, Yoo Shin Choi, Beom Gyu Kim, Yong Gum Park
International Journal of Colorectal Disease.2018; 33(4): 441. CrossRef - Abrupt Increase in Rate of Imipenem Resistance in Acinetobacter baumannii Complex Strains Isolated from General Hospitals in Korea and Correlation With Carbapenem Administration During 2002?2013
Young Ah Kim, Yoon Soo Park, Taemi Youk, Hyukmin Lee, Kyungwon Lee
Annals of Laboratory Medicine.2018; 38(2): 179. CrossRef - Factors associated with in-hospital mortality among critically ill surgical patients with multidrug-resistant Gram-negative infections
Andrew S. Jarrell, Rachel M. Kruer, Loredana Diana Berescu, Peter J. Pronovost, Julie B. Trivedi
Journal of Critical Care.2018; 43: 321. CrossRef - Émergence de la résistance à la colistine chez les entérobactéries : une brèche dans le dernier rempart contre la pan-résistance !
L. Dortet, R. Bonnin, A. Jousset, L. Gauthier, T. Naas
Journal des Anti-infectieux.2016; 18(4): 139. CrossRef - Antimicrobial Resistance and the Presence of Virulence Genes in Escherichia coli Strains Isolated from Ruditapes philippinarum in Gomso Bay, Korea
Tae-Ok Kim, In-Seon Eom, Kwang-Ho Park, Kwon-Sam Park
Korean Journal of Fisheries and Aquatic Sciences.2016; 49(6): 800. CrossRef
- Analysis of Predictive Factors of Antibiotic Resistant Pseudomonas aeruginosa in Children with Acute Appendicitis
- DsbM, a Novel Disulfide Oxidoreductase Affects Aminoglycoside Resistance in Pseudomonas aeruginosa by OxyR-Regulated Response
- Xuehan Wang , Mingxuan Li , Liwei Liu , Rui Mou , Xiuming Zhang , Yanling Bai , Haijin Xu , Mingqiang Qiao
- J. Microbiol. 2012;50(6):932-938. Published online December 30, 2012
- DOI: https://doi.org/10.1007/s12275-012-2177-3
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- 7 Scopus
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Abstract
- A Pseudomonas aeruginosa mutant strain M122 was isolated from a Mu transposon insertion mutant library. In our prophase research, we have found that PA0058, a novel gene encodes a 234-residue conserved protein, was disrupted in the M122 mutant. In this study, the bacteriostatic experiment in vitro indicates that M122 has abnormally high aminoglycoside resistance. We expressed PA0058 in E. coli and found that PA0058 oxidizes and reduces disulfide. This biochemical characterization suggests that PA0058 is a novel disulfide oxidoreductase. Hence, the protein was designated as DsbM. Microarray analysis of the M122 mutant showed its unusual phenotype might be related to the bacterial antioxidant defense system mediated by the oxyR regulon. Meanwhile, we detected –SH content in the periplasm of M122 and wild strain and found a lower –SH/S–S ratio in M122. Therefore, we consider that the loss of dsbM function decreased the –SH/S–S ratio, which then prolongs the OxyR-regulated response, thereby conferring high aminoglycoside resistance to the M122 mutant strain. Our findings have important implications for understanding the mechanisms underlying aminoglycoside resistance in P. aeruginosa.
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