MSK
Search
- Page Path
- HOME > Search
Journal Articles
- Identification and Characterization of HEPN‑MNT Type II TA System from Methanothermobacter thermautotrophicus ΔH
- Wonho Choi , Anoth Maharjan , Hae Gang Im , Ji-Young Park , Jong-Tae Park , Jung-Ho Park
- J. Microbiol. 2023;61(4):411-421. Published online April 18, 2023
- DOI: https://doi.org/10.1007/s12275-023-00041-9
- 60 View
- 0 Download
- 1 Web of Science
- 1 Crossref
-
Abstract
- Toxin-antitoxin (TA) systems are widespread in bacteria and archaea plasmids and genomes to regulate DNA replication, gene transcr!ption, or protein translation. Higher eukaryotic and prokaryotic nucleotide-binding (HEPN) and minimal nucleotidyltransferase (MNT) domains are prevalent in prokaryotic genomes and constitute TA pairs. However, three gene pairs (MTH304/305, 408/409, and 463/464) of Methanothermobacter thermautotropicus ΔH HEPN-MNT family have not been studied as TA systems. Among these candidates, our study characterizes the MTH463/MTH464 TA system. MTH463 expression inhibited Escherichia coli growth, whereas MTH464 did not and blocked MTH463 instead. Using site-directed MTH463 mutagenesis, we determined that amino acids R99G, H104A, and Y106A from the R[ɸX]4-6H motif are involved with MTH463 cell toxicity. Furthermore, we established that purified MTH463 could degrade MS2 phage RNA, whereas purified MTH464 neutralized MTH463 activity in vitro. Our results indicate that the endonuclease toxin MTH463 (encoding a HEPN domain) and its cognate antitoxin MTH464 (encoding the MNT domain) may act as a type II TA system in M. thermautotropicus ΔH. This study provides initial and essential information studying TA system functions, primarily archaea HEPN-MNT family.
-
Citations
Citations to this article as recorded by
- Extensive Genomic Rearrangement of Catalase-Less Cyanobloom-Forming Microcystis aeruginosa in Freshwater Ecosystems
Minkyung Kim, Jaejoon Jung, Wonjae Kim, Yerim Park, Che Ok Jeon, Woojun Park
Journal of Microbiology.2024; 62(11): 933. CrossRef
- Extensive Genomic Rearrangement of Catalase-Less Cyanobloom-Forming Microcystis aeruginosa in Freshwater Ecosystems
- Vibrio vulnificus PlpA facilitates necrotic host cell death induced by the pore forming MARTX toxin
- Changyi Cho , Sanghyeon Choi , Myung Hee Kim , Byoung Sik Kim
- J. Microbiol. 2022;60(2):224-233. Published online February 1, 2022
- DOI: https://doi.org/10.1007/s12275-022-1448-x
- 61 View
- 0 Download
- 9 Web of Science
- 6 Crossref
-
Abstract
- Opportunistic pathogen Vibrio vulnificus causes severe systemic infection in humans with high mortality. Although multiple exotoxins have been characterized in V. vulnificus, their interactions and potential synergistic roles in pathogen-induced host cell death have not been investigated previously. By employing a series of multiple exotoxin deletion mutants, we investigated whether specific exotoxins of the pathogen functioned together to achieve severe and rapid necrotic cell death. Human epithelial cells treated with V. vulnificus with a plpA deletion background exhibited an unusually prolonged cell blebbing, suggesting the importance of PlpA, a phospholipase A2, in rapid necrotic cell death by this pathogen. Additional deletion of the rtxA gene encoding the multifunctional autoprocessing repeats-in-toxin (MARTX) toxin did not result in necrotic cell blebs. However, if the rtxA gene was engineered to produce an effector-free MARTX toxin, the cell blebbing was observed, indicating that the pore forming activity of the MARTX toxin is sufficient, but the MARTX toxin effector domains are not necessary, for the blebbing. When a recombinant PlpA was treated on the blebbed cells, the blebs were completely disrupted. Consistent with this, MARTX toxin-pendent rapid release of cytosolic lactate dehydrogenase was significantly delayed in the plpA deletion background. Mutations in other exotoxins such as elastase, cytolysin/hemolysin, and/or extracellular metalloprotease did not affect the bleb formation or disruption. Together, these findings indicate that the pore forming MARTX toxin and the phospholipase A2, PlpA, cooperate sequentially to achieve rapid necrotic cell death by inducing cell blebbing and disrupting the blebs, respectively.
-
Citations
Citations to this article as recorded by- Genome-wide phenotypic profiling of transcription factors and identification of novel targets to control the virulence of Vibrio vulnificus
Dayoung Sung, Garam Choi, Minji Ahn, Hokyung Byun, Tae Young Kim, Hojun Lee, Zee-Won Lee, Ji Yong Park, Young Hyun Jung, Ho Jae Han, Sang Ho Choi
Nucleic Acids Research.2024;[Epub] CrossRef - Vibrio-infecting bacteriophages and their potential to control biofilm
Ana Cevallos-Urena, Jeong Yeon Kim, Byoung Sik Kim
Food Science and Biotechnology.2023; 32(12): 1719. CrossRef -
Pathogenic Mechanism of
Vibrio Vulnificus
Infection
Kun Lu, Yang Li, Rui Chen, Hua Yang, Yong Wang, Wei Xiong, Fang Xu, Qijun Yuan, Haihui Liang, Xian Xiao, Renqiang Huang, Zhipeng Chen, Chunou Tian, Songqing Wang
Future Microbiology.2023; 18(6): 373. CrossRef - Functional conservation of specialized ribosomes bearing genome-encoded variant rRNAs in Vibrio species
Younkyung Choi, Eunkyoung Shin, Minho Lee, Ji-Hyun Yeom, Kangseok Lee, Bashir Sajo Mienda
PLOS ONE.2023; 18(12): e0289072. CrossRef - Complex regulatory networks of virulence factors in Vibrio vulnificus
Garam Choi, Sang Ho Choi
Trends in Microbiology.2022; 30(12): 1205. CrossRef - MARTX toxin of Vibrio vulnificus induces RBC phosphatidylserine exposure that can contribute to thrombosis
Han Young Chung, Yiying Bian, Kyung-Min Lim, Byoung Sik Kim, Sang Ho Choi
Nature Communications.2022;[Epub] CrossRef
- Genome-wide phenotypic profiling of transcription factors and identification of novel targets to control the virulence of Vibrio vulnificus
- Analyses of DNA double-strand break repair pathways in tandem arrays of HXT genes of Saccharomyces cerevisiae
- Ju-Hee Choi , Ye-Seul Lim , Min-Ku Kim , Sung-Ho Bae
- J. Microbiol. 2020;58(11):957-966. Published online October 30, 2020
- DOI: https://doi.org/10.1007/s12275-020-0461-1
- 61 View
- 0 Download
- 4 Web of Science
- 4 Crossref
-
Abstract
- Eukaryotic genomes contain numerous homologous repeat sequences including redundant genes with divergent homology that can be potential recombination targets. Recombination between divergent sequences is rare but poses a substantial threat to genome stability. The hexose transporter (HXT) gene family shares high sequence similarities at both protein and DNA levels, and some members are placed close together in tandem arrays. In this study, we show that spontaneous interstitial deletions occur at significantly high rates in HXT gene clusters, resulting in chimeric HXT sequences that contain a single junction point. We also observed that DNA double-strand breaks created between HXT genes produce primarily interstitial deletions, whereas internal cleavage of the HXT gene resulted in gene conversions as well as deletion products. Interestingly, interstitial deletions were less constrained by sequence divergence than gene conversion. Moreover, recombination-defective mutations differentially affected the survival frequency. Mutations that impair single-strand annealing (SSA) pathway greatly reduced the survival frequency by 10–1,000-fold, whereas disruption of Rad51-dependent homologous recombination exhibited only modest reduction. Our results indicate that recombination in the tandemly repeated HXT genes occurs primarily via SSA pathway.
-
Citations
Citations to this article as recorded by- Deletion of IRC19 Causes Defects in DNA Double-Strand Break Repair Pathways in Saccharomyces cerevisiae
Ju-Hee Choi, Oyungoo Bayarmagnai, Sung-Ho Bae
Journal of Microbiology.2024; 62(9): 749. CrossRef - A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris
Xiang Wang, Yi Li, Zhehao Jin, Xiangjian Liu, Xiang Gao, Shuyuan Guo, Tao Yu
Synthetic and Systems Biotechnology.2023; 8(3): 445. CrossRef - Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms
Jucan Gao, Cuifang Ye, Jintao Cheng, Lihong Jiang, Xinghao Yuan, Jiazhang Lian
ACS Synthetic Biology.2022; 11(2): 547. CrossRef - Effects of the loss of mismatch repair genes on single-strand annealing between divergent sequences in Saccharomyces cerevisiae
Ye-Seul Lim, Ju-Hee Choi, Kyu-Jin Ahn, Min-Ku Kim, Sung-Ho Bae
Journal of Microbiology.2021; 59(4): 401. CrossRef
- Deletion of IRC19 Causes Defects in DNA Double-Strand Break Repair Pathways in Saccharomyces cerevisiae
- IgG and IgM responses to human papillomavirus L1 virus-like particle as a function of dosing schedule and vaccine formulation
- Min-Hye Park , Ji Won You , Hyoung Jin Kim , Hong-Jin Kim
- J. Microbiol. 2019;57(9):821-827. Published online August 27, 2019
- DOI: https://doi.org/10.1007/s12275-019-9308-z
- 58 View
- 0 Download
- 4 Web of Science
- 4 Crossref
-
Abstract
-
Most commercialized virus-like particle (VLP) vaccines use
aluminum salt as adjuvant, even though VLPs provoke adequate
antibody responses without adjuvant. We do not have
detailed knowledge of how adjuvant affects the profile of anti-
VLP antibodies. Meanwhile, there is evidence that differences
between vaccination protocols influence the glycosylation of
antibodies, which may alter their effector functions. In the
present study a murine model was used to investigate the effects
of dosing schedule and adjuvant on the antibody profiles
and glycosylation levels of antigen-specific antibody responses
to human papillomavirus type 16 L1 (HPV16 L1)
VLPs. Mice received subcutaneously 2,000 ng of antigen divided
into 4 or 7 doses. The HPV16 L1 VLPs elicited > 4 log10
anti-HPV16 L1 IgG titers without adjuvant, and aluminum
hydroxide as adjuvant increased IgG titers 1.3- to 4-fold and
reduced the anti-HPV16 L1 IgG2a / anti-HPV16 L1 IgG1
ratio value (use of aluminum hydroxide reduced the ratio of
the IgG2a). Immunization with HPV16 L1 VLPs in combination
with Freund’s adjuvant enhanced IgG titers 5- to 12-
fold. Seven-dose immunization markedly increased anti-
HPV16 L1 IgM titers compared to four-dose immunization,
as well as increasing the proportion of glycosylated antibodies.
Our results suggest that antibody glycosylation can be controlled
immunologically, and IgG and IgM profiles and glycosylation
profiles of the vaccine-induced antibodies can be
used as indicators reflecting the vaccine characteristics. These
results
indicate that the HPV16 L1 VLP dosing schedule can affect the quality of antigen-specific antibody responses. We suggest that dosing schedules should be noted in vaccination protocols for VLP-based vaccines. -
Citations
Citations to this article as recorded by- Human papillomavirus vaccines: organisation and experience of preclinical studies
A. S. Korovkin, T. N. Nikitina, T. Yu. Kozlova, D. V. Gorenkov, A. R. Volgin
Biological Products. Prevention, Diagnosis, Treatment.2024; 24(3): 243. CrossRef - Chimeric Hepatitis B core virus-like particles harboring SARS-CoV2 epitope elicit a humoral immune response in mice
Sima Sazegari, Malihe Akbarzadeh Niaki, Alireza Afsharifar, Ali Niazi, Abdollah Derakhshandeh, Maryam Moradi Vahdat, Farshad Hemmati, Mohammad Hadi Eskandari
Microbial Cell Factories.2023;[Epub] CrossRef - Anti-JMH alloantibody in inherited JMH-negative patients leads to immunogenic destruction of JMH-positive RBCs
Zhaohu Yuan, Yaming Wei, Xiaojie Chen, Shufei He, Kui Cai, Minglu Zhong, Huiying Huang, Xinxin Tong, Zhen Liu, Xuexin Yang
Clinical and Experimental Immunology.2021; 205(2): 182. CrossRef - Prevalence of antibodies against a cyclic peptide mimicking the FG loop of the human papillomavirus type 16 capsid among Tunisian women
Elham Hassen, Devendra Bansal, Randa Ghdira, Anouar Chaieb, Hedi Khairi, Abdelfattah Zakhama, Sami Remadi, Johan Hoebeke, Ali A. Sultan, Lotfi Chouchane
Journal of Translational Medicine.2020;[Epub] CrossRef
- Human papillomavirus vaccines: organisation and experience of preclinical studies
Review
- [Minireview] Antibiotic resistance of pathogenic Acinetobacter species and emerging combination therapy
- Bora Shin , Woojun Park
- J. Microbiol. 2017;55(11):837-849. Published online October 27, 2017
- DOI: https://doi.org/10.1007/s12275-017-7288-4
- 56 View
- 0 Download
- 38 Crossref
-
Abstract
- The increasing antibiotic resistance of Acinetobacter species in both natural and hospital environments has become a serious problem worldwide in recent decades. Because of both intrinsic and acquired antimicrobial resistance (AMR) against last-resort antibiotics such as carbapenems, novel therapeutics are urgently required to treat Acinetobacter-associated infectious diseases. Among the many pathogenic Acinetobacter species, A. baumannii has been reported to be resistant to all classes of antibiotics and contains many AMR genes, such as blaADC (Acinetobacter-derived cephalosporinase). The AMR of pathogenic Acinetobacter species is the result of several different mechanisms, including active efflux pumps, mutations in antibiotic targets, antibiotic modification, and low antibiotic membrane permeability. To overcome the limitations of existing drugs, combination theraphy that can increase the activity of antibiotics should be considered in the treatment of Acinetobacter infections. Understanding the molecular mechanisms behind Acinetobacter AMR resistance will provide vital information for drug development and therapeutic strategies using combination treatment. Here, we summarize the classic mechanisms of Acinetobacter AMR, along with newly-discovered genetic AMR factors and currently available antimicrobial adjuvants that can enhance drug efficacy in the treatment of A. baumannii infections.
-
Citations
Citations to this article as recorded by- Disruption of bacterial interactions and community assembly in Babesia-infected Haemaphysalis longicornis following antibiotic treatment
Myriam Kratou, Apolline Maitre, Lianet Abuin-Denis, Elianne Piloto-Sardiñas, Ivan Corona-Guerrero, Ana Laura Cano-Argüelles, Alejandra Wu-Chuang, Timothy Bamgbose, Consuelo Almazan, Juan Mosqueda, Dasiel Obregón, Lourdes Mateos-Hernández, Mourad Ben Said,
BMC Microbiology.2024;[Epub] CrossRef - A 19-year longitudinal study to characterize carbapenem-nonsusceptible Acinetobacter isolated from patients with bloodstream infections and the contribution of conjugative plasmids to carbapenem resistance and virulence
Pek Kee Chen, Yi-Tzu Lee, Chia-Ying Liu, Tran Thi Dieu Thuy, Kieu Anh, Jiunn-Jong Wu, Chun-Hsing Liao, Yu-Tsung Huang, Yu-Chen Chen, Cheng-Yen Kao
Journal of Microbiology, Immunology and Infection.2024; 57(2): 288. CrossRef - MOLECULAR ANALYSIS OF THE MCR-1 GENE IN PSEUDOMONAS AERUGINOSA AND ACINETOBACTER BAUMANII STRAINS
Ömer Akgül
Ankara Universitesi Eczacilik Fakultesi Dergisi.2024; 48(3): 21. CrossRef - Effect of Phenylalanine–Arginine Beta-Naphthylamide on the Values of Minimum Inhibitory Concentration of Quinolones and Aminoglycosides in Clinical Isolates of Acinetobacter baumannii
Stefany Plasencia-Rebata, Saul Levy-Blitchtein, Juana del Valle-Mendoza, Wilmer Silva-Caso, Isaac Peña-Tuesta, William Vicente Taboada, Fernando Barreda Bolaños, Miguel Angel Aguilar-Luis
Antibiotics.2023; 12(6): 1071. CrossRef - A comprehensive genomic analysis provides insights on the high environmental adaptability of Acinetobacter strains
Yang Zhao, Hua-Mei Wei, Jia-Li Yuan, Lian Xu, Ji-Quan Sun
Frontiers in Microbiology.2023;[Epub] CrossRef - Acinetobacter baumannii in blood-borne and central nervous system infections in intensive care unit children: molecular and genetic characteristics and clinical significance
Zulfirya Z. Sadeeva, Irina E. Novikova, Natalia M. Alyabyeva, Anna V. Lazareva, Tatiana M. Komyagina, Olga V. Karaseva, Marina G. Vershinina, Andrey P. Fisenko
Russian Journal of Infection and Immunity.2023; 13(2): 289. CrossRef - Herbal Products and Their Active Constituents Used Alone and in Combination with Antibiotics against Multidrug-Resistant Bacteria
Anna Herman, Andrzej P. Herman
Planta Medica.2023; 89(02): 168. CrossRef - A Systematic Review of Culture-Based Methods for Monitoring Antibiotic-Resistant Acinetobacter, Aeromonas, and Pseudomonas as Environmentally Relevant Pathogens in Wastewater and Surface Water
Erin G. Milligan, Jeanette Calarco, Benjamin C. Davis, Ishi M. Keenum, Krista Liguori, Amy Pruden, Valerie J. Harwood
Current Environmental Health Reports.2023; 10(2): 154. CrossRef - Gold nanoparticle-DNA aptamer-assisted delivery of antimicrobial peptide effectively inhibits Acinetobacter baumannii infection in mice
Jaeyeong Park, Eunkyoung Shin, Ji-Hyun Yeom, Younkyung Choi, Minju Joo, Minho Lee, Je Hyeong Kim, Jeehyeon Bae, Kangseok Lee
Journal of Microbiology.2022; 60(1): 128. CrossRef - In vitro study to evaluate the antimicrobial activity of various multifunctional cosmetic ingredients and chlorphenesin on bacterial species at risk in the cosmetic industry
Benjamin Youenou, Amandine Chauviat, Chrisse Ngari, Valérie Poulet, Sylvie Nazaret
Journal of Applied Microbiology.2022; 132(2): 933. CrossRef - Profiles of Microbial Community and Antibiotic Resistome in Wild Tick Species
Nana Wei, Jinmiao Lu, Yi Dong, Shibo Li, Jack A. Gilbert
mSystems.2022;[Epub] CrossRef - Conventional and Real-Time PCR Targeting blaOXA Genes as Reliable Methods for a Rapid Detection of Carbapenem-Resistant Acinetobacter baumannii Clinical Strains
Dagmara Depka, Agnieszka Mikucka, Tomasz Bogiel, Mateusz Rzepka, Patryk Zawadka, Eugenia Gospodarek-Komkowska
Antibiotics.2022; 11(4): 455. CrossRef - In vitro synergistic activity of colistin and teicoplanin combination against multidrug-resistant Acinetobacter spp
Osama Mohamed Samy Mohamed Rady, Laila El-Attar, Amira Amine
The Journal of Antibiotics.2022; 75(3): 181. CrossRef -
Biogenic silver nanoparticle (Bio‐AgNP) has an antibacterial effect against carbapenem‐resistant
Acinetobacter baumannii
with synergism and additivity when combined with polymyxin B
Suzane Olachea Allend, Marcelle Oliveira Garcia, Kamila Furtado da Cunha, Déborah Trota Farias de Albernaz, Mirian Elert da Silva, Rodrigo Yudi Ishikame, Luciano Aparecido Panagio, Gerson Nakazaro, Guilherme Fonseca Reis, Daniela Brayer Pereira, Daiane Dr
Journal of Applied Microbiology.2022; 132(2): 1036. CrossRef - RapidResa Polymyxin Acinetobacter NP® Test for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii
Maxime Bouvier, Mustafa Sadek, Stefano Pomponio, Fernando D’Emidio, Laurent Poirel, Patrice Nordmann
Antibiotics.2021; 10(5): 558. CrossRef - Rapid detection of carbapenemase-producing Pseudomonas spp. using the NitroSpeed-Carba NP test
Mustafa Sadek, Laurent Poirel, Patrice Nordmann
Diagnostic Microbiology and Infectious Disease.2021; 99(3): 115280. CrossRef - Gain and loss of antibiotic resistant genes in multidrug resistant bacteria: One Health perspective
Misung Kim, Jaeeun Park, Mingyeong Kang, Jihye Yang, Woojun Park
Journal of Microbiology.2021; 59(6): 535. CrossRef - OXA-23 and OXA-40 producing carbapenem-resistant Acinetobacter baumannii in Central Illinois
Janak Koirala, Isha Tyagi, Lohitha Guntupalli, Sameena Koirala, Udita Chapagain, Christopher Quarshie, Sami Akram, Vidya Sundareshan, Sajan Koirala, Jerry Lawhorn, Yohei Doi, Michael Olson
Diagnostic Microbiology and Infectious Disease.2020; 97(1): 114999. CrossRef - Rapid Polymyxin/Pseudomonas NP test for rapid detection of polymyxin susceptibility/resistance in Pseudomonas aeruginosa
Mustafa Sadek, Camille Tinguely, Laurent Poirel, Patrice Nordmann
European Journal of Clinical Microbiology & Infectious Diseases.2020; 39(9): 1657. CrossRef - Stress responses linked to antimicrobial resistance in Acinetobacter species
Bora Shin, Chulwoo Park, Woojun Park
Applied Microbiology and Biotechnology.2020; 104(4): 1423. CrossRef - Carbapenemases: Transforming Acinetobacter baumannii into a Yet More Dangerous Menace
Maria Soledad Ramirez, Robert A. Bonomo, Marcelo E. Tolmasky
Biomolecules.2020; 10(5): 720. CrossRef - Pharmacokinetics, Safety, and Tolerability of Intravenous Durlobactam and Sulbactam in Subjects with Renal Impairment and Healthy Matched Control Subjects
John O’Donnell, Richard A. Preston, Grigor Mamikonyan, Emily Stone, Robin Isaacs
Antimicrobial Agents and Chemotherapy.2019;[Epub] CrossRef - Efficient Delivery of Antisense Oligonucleotides by an Amphipathic Cell-Penetrating Peptide in Acinetobacter baumannii
Zhou Chen, Dan Nie, Yue Hu, Mingkai Li, Zheng Hou, Xinggang Mao, Xiaoxing Luo, Xiaoyan Xue
Current Drug Delivery.2019; 16(8): 728. CrossRef - Restoring the activity of the antibiotic aztreonam using the polyphenol epigallocatechin gallate (EGCG) against multidrug-resistant clinical isolates of Pseudomonas aeruginosa
Jonathan W. Betts, Michael Hornsey, Paul G. Higgins, Kai Lucassen, Julia Wille, Francisco J. Salguero, Harald Seifert, Roberto M. La Ragione
Journal of Medical Microbiology .2019; 68(10): 1552. CrossRef - Antibiotic-resistant clones in Gram-negative pathogens: presence of global clones in Korea
Kwan Soo Ko
Journal of Microbiology.2019; 57(3): 195. CrossRef - Alternative fate of glyoxylate during acetate and hexadecane metabolism in Acinetobacter oleivorans DR1
Chulwoo Park, Bora Shin, Woojun Park
Scientific Reports.2019;[Epub] CrossRef - Nationwide surveillance of antimicrobial resistance among clinically important Gram-negative bacteria, with an emphasis on carbapenems and colistin: Results from the Surveillance of Multicenter Antimicrobial Resistance in Taiwan (SMART) in 2018
Yu-Lin Lee, Min-Chi Lu, Pei-Lan Shao, Po-Liang Lu, Yen-Hsu Chen, Shu-Hsing Cheng, Wen-Chien Ko, Chi-Ying Lin, Ting-Shu Wu, Muh-Yong Yen, Lih-Shinn Wang, Chang-Pan Liu, Wen-Sen Lee, Zhi-Yuan Shi, Yao-Shen Chen, Fu-Der Wang, Shu-Hui Tseng, Chao-Nan Lin, Yu-
International Journal of Antimicrobial Agents.2019; 54(3): 318. CrossRef - The use of polymyxins to treat carbapenem resistant infections in neonates and children
Reenu Thomas, Sithembiso Velaphi, Sally Ellis, A. Sarah Walker, Joseph F. Standing, Paul Heath, Mike Sharland, Daniele Dona’
Expert Opinion on Pharmacotherapy.2019; 20(4): 415. CrossRef - Plasmid-mediated mcr-1 gene in Acinetobacter baumannii and Pseudomonas aeruginosa: first report from Pakistan
Fareeha Hameed, Muhammad Asif Khan, Hafsah Muhammad, Tahir Sarwar, Hazrat Bilal, Tayyab Ur Rehman
Revista da Sociedade Brasileira de Medicina Tropical.2019;[Epub] CrossRef - Identification of factors needed by a clinical isolate of Acinetobacter baumannii to resist antibacterial compounds
Celena M. Gwin, Natalia Prakash, Nathan W. Rigel
BIOS.2019; 90(3): 149. CrossRef - A Resazurin Reduction-Based Assay for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii and Pseudomonas aeruginosa
Mathilde Lescat, Laurent Poirel, Camille Tinguely, Patrice Nordmann, Nathan A. Ledeboer
Journal of Clinical Microbiology.2019;[Epub] CrossRef - Expansion of antibacterial spectrum of xanthorrhizol against Gram-negatives in combination with PMBN and food-grade antimicrobials
Man Su Kim, Ha-Rim Kim, Haebom Kim, Soo-Keun Choi, Chang-Hwan Kim, Jae-Kwan Hwang, Seung-Hwan Park
Journal of Microbiology.2019; 57(5): 405. CrossRef - Carbapenem-resistant Acinetobacter baumannii in patients with burn injury: A systematic review and meta-analysis
William Gustavo Lima, Geisa Cristina Silva Alves, Cristina Sanches, Simone Odília Antunes Fernandes, Magna Cristina de Paiva
Burns.2019; 45(7): 1495. CrossRef - Performances of the Rapid Polymyxin Acinetobacter and Pseudomonas Tests for Colistin Susceptibility Testing
Mathilde Lescat, Laurent Poirel, Aurélie Jayol, Patrice Nordmann
Microbial Drug Resistance.2019; 25(4): 520. CrossRef - In vitro activities of ceftazidime/avibactam alone or in combination with antibiotics against multidrug-resistant Acinetobacter baumannii isolates
Emel Mataracı Kara, Mesut Yılmaz, Berna Özbek Çelik
Journal of Global Antimicrobial Resistance.2019; 17: 137. CrossRef - Zoonotic Diseases and Phytochemical Medicines for Microbial Infections in Veterinary Science: Current State and Future Perspective
Bora Shin, Woojun Park
Frontiers in Veterinary Science.2018;[Epub] CrossRef - A formidable foe: carbapenem-resistant Acinetobacter baumannii and emerging nonantibiotic therapies
Richard R. Watkins
Expert Review of Anti-infective Therapy.2018; 16(8): 591. CrossRef - Plasma and Intrapulmonary Concentrations of ETX2514 and Sulbactam following Intravenous Administration of ETX2514SUL to Healthy Adult Subjects
Keith A. Rodvold, Mark H. Gotfried, Robin D. Isaacs, John P. O'Donnell, Emily Stone
Antimicrobial Agents and Chemotherapy.2018;[Epub] CrossRef
- Disruption of bacterial interactions and community assembly in Babesia-infected Haemaphysalis longicornis following antibiotic treatment
Research Support, Non-U.S. Gov't
- Adjuvant Efficacy of mOMV against Avian Influenza Virus Infection in Mice
- Byeong-Jae Lee , Sang-Ho Lee , Min-Suk Song , Philippe Noriel Q. Pascua , Hyeok-il Kwon , Su-Jin Park , Eun-Ha Kim , Arun Decano , Se Mi Kim , Gyo Jin Lim , Doo-Jin Kim , Kyu-Tae Chang , Sang-Hyun Kim , Young Ki Choi
- J. Microbiol. 2013;51(5):682-688. Published online October 31, 2013
- DOI: https://doi.org/10.1007/s12275-013-3411-3
- 31 View
- 0 Download
- 1 Scopus
-
Abstract
- Highly pathogenic avian influenza H5N1 viruses are found chiefly in birds and have caused severe disease and death in infected humans. Development of influenza vaccines capable of inducing heterosubtypic immunity against a broad range of influenza viruses is the best option for the preparedness, since vaccination remains the principal method in controlling influenza viral infections. Here, a mOMV-adjuvanted recombinant H5N2 (rH5N2) whole virus antigen vaccine with A/Environment/Korea/W149/06(H5N1)-derived H5 HA and A/Chicken/Korea/ma116/04(H9N2)-derived N2 NA in the backbone of A/Puerto Rico/8/34(H1N1) was prepared and generated by reverse genetics. Groups of mice were vaccinated by a prime-boost regime with the rH5N2 vaccine (1.75 μg of HA with/without 10 μg mOMV or aluminum hydroxide adjuvant for comparison). At two weeks post-immunizations, vaccinated mice were challenged with lethal doses of 103.5 EID50/ml of H5N1 or H9N2 avian influenza viruses, and were monitored for 15 days. Both mOMV- and alum-adjuvant vaccine groups had high survival rates after H5N1 infection and low levels of body weight changes compared to control groups. Interestingly, the mOMV-adjuvanted group induced better cross-reactive antibody responses serologically and promoted cross-protectivity against H5N1 and H9N2 virus challenges. Our results suggest that mOMV could be used as a vaccine adjuvant in the development of effective vaccines used to control influenza A virus transmission.
First
Prev
TOP
