MSK
Search
- Page Path
- HOME > Search
- The key pathways and genes related to oncolytic Newcastle disease virus-induced phenotypic changes in ovarian cancer cells
- Wei Song, Yuan Yuan, Fangfang Cao, Huazheng Pan, Yaqing Liu
- J. Microbiol. 2025;63(4):e2411018. Published online April 29, 2025
- DOI: https://doi.org/10.71150/jm.2411018
- 56 View
- 3 Download
-
Abstract
PDF
Supplementary Material -
The poor prognosis and high recurrence rate of ovarian cancer highlight the urgent need to develop new therapeutic strategies. Oncolytic Newcastle disease virus (NDV) can kill cancer cells directly and regulate innate and adaptive immunity. In this study, ovarian cancer cells infected with or without velogenic NDV-BJ were subjected to a CCK-8 assay for detecting cell proliferation, flow cytometry for detecting the cell cycle and apoptosis, and wound healing and transwell assays for detecting cell migration and invasion. Transcriptomic sequencing was conducted to identify the differentially expressed genes (DEGs). GO and KEGG enrichment analyses were performed to explore the mechanism underlying the oncolytic effect of NDV on ovarian cancer cells. The results showed that infection with NDV inhibited ovarian cancer cell proliferation, migration, and invasion; disrupted the cell cycle; and promoted apoptosis. Compared with those in negative control cells, the numbers of upregulated and downregulated genes in ovarian cancer cells infected with NDV were 1,499 and 2,260, respectively. Thirteen KEGG pathways related to cell growth and death, cell mobility, and signal transduction were significantly enriched. Among these pathways, 48 DEGs, especially SESN2, HLA B/C/E, GADD45B, and RELA, that may be involved in the oncolytic process were screened, and qPCR analysis verified the reliability of the transcription data. This study discovered some key pathways and genes related to oncolytic NDV-induced phenotypic changes in ovarian cancer cells, which will guide our future research directions and help further explore the specific mechanisms by which infection with NDV suppresses ovarian cancer development.
- Altered mRNA Levels of MOV10, A3G, and IFN-α in Patients with Chronic Hepatitis B
- Zhi-Wei Song , Yan-Xiu Ma , Li-Juan Fu , Bao-qing Fu , Xu Teng , Si-Jia Chen , Wei-Zhen Xu , Hong-Xi Gu
- J. Microbiol. 2014;52(6):510-514. Published online May 29, 2014
- DOI: https://doi.org/10.1007/s12275-014-3467-8
- 58 View
- 0 Download
- 8 Crossref
-
Abstract
- To explore the relationship of the MOV10, A3G, and IFN-α mRNA levels with chronic hepatitis B virus (HBV) infection, Blood samples from 96 patients with chronic hepatitis B (CHB) and 21 healthy individuals as control were collected. HBV DNA load and aminotransferase in the serum were tested using real time PCR and velocity methods, respectively. The MOV10, A3G, and IFN-α mRNA levels in the peripheral blood mononuclear cells (PBMC) were examined through qRT-PCR. The MOV10, A3G, and IFN-α mRNA levels in CHB group was significantly lower than those in the control group (P<0.01, P<0.05, P<0.01, respectively). The A3G mRNA level in the high-HBV DNA load group was lower than that in the low-HBV DNA load group (P<0.05). However, no statistical difference was found in the MOV10 and IFN-α mRNA levels between the two HBV DNA load groups. Furthermore, the MOV10 mRNA level showed positive correlation with IFN-α in the control group. These results indicated that the expression of the innate immune factors MOV10, A3G, and IFN-α is affected by chronic HBV infection.
-
Citations
Citations to this article as recorded by
- Hepatic exosomes with declined MiR‐27b‐3p trigger RIG‐I/TBK1 signal pathway in macrophages
Jie You, Wenyu Wu, Mengxin Lu, Yanghao Xie, Rui Miao, Misi Gu, Dong Xi, Weiming Yan, Di Wu, Xiaojing Wang, Tao Chen, Qin Ning, Meifang Han
Liver International.2022; 42(7): 1676. CrossRef - Unwinding the roles ofRNAhelicaseMOV10
Aatiqa Nawaz, Temirlan Shilikbay, Geena Skariah, Stephanie Ceman
WIREs RNA.2022;[Epub] CrossRef - Evolutionary and Expression Analysis of MOV10 and MOV10L1 Reveals Their Origin, Duplication and Divergence
Shuaiqi Yang, Xiangmin Zhang, Xianpeng Li, Xiu Yin, Lei Teng, Guangdong Ji, Hongyan Li
International Journal of Molecular Sciences.2022; 23(14): 7523. CrossRef - Roles of APOBEC3 in hepatitis B virus (HBV) infection and hepatocarcinogenesis
Yuan Zhang, Xiaorong Chen, Yajuan Cao, Zongguo Yang
Bioengineered.2021; 12(1): 2074. CrossRef - Profiling of LINE-1-Related Genes in Hepatocellular Carcinoma
Tomoyuki Honda, Md. Arifur Rahman
International Journal of Molecular Sciences.2019; 20(3): 645. CrossRef - Host Protein Moloney Leukemia Virus 10 (MOV10) Acts as a Restriction Factor of Influenza A Virus by Inhibiting the Nuclear Import of the Viral Nucleoprotein
Junsong Zhang, Feng Huang, Likai Tan, Chuan Bai, Bing Chen, Jun Liu, Juanran Liang, Chao Liu, Shaoying Zhang, Gen Lu, Yuan Chen, Hui Zhang, S. López
Journal of Virology.2016; 90(8): 3966. CrossRef - The role of Moloney leukemia virus 10 in hepatitis B virus expression in hepatoma cells
Yan-Xiu Ma, Di Li, Li-Juan Fu, Bao-qing Fu, Si-Jia Chen, Wei-Zhen Xu, Xu Teng, Zhi-wei Song, Hong-Xi Gu
Virus Research.2015; 197: 85. CrossRef - RNA helicase MOV10 functions as a co-factor of HIV-1 Rev to facilitate Rev/RRE-dependent nuclear export of viral mRNAs
Feng Huang, Junsong Zhang, Yijun Zhang, Guannan Geng, Juanran Liang, Yingniang Li, Jingliang Chen, Chao Liu, Hui Zhang
Virology.2015; 486: 15. CrossRef
- Hepatic exosomes with declined MiR‐27b‐3p trigger RIG‐I/TBK1 signal pathway in macrophages
First
Prev
TOP
