MSK
Search
- Page Path
- HOME > Search
Research Support, Non-U.S. Gov't
- Cys-92, Cys-95, and the C-Terminal 12 Residues of the Vibrio harveyi Ferric Uptake Regulator (Fur) are Functionally Inessential
- Kun Sun , Shuang Cheng , Min Zhang , Fang Wang , Li Sun
- J. Microbiol. 2008;46(6):670-680. Published online December 24, 2008
- DOI: https://doi.org/10.1007/s12275-008-0113-3
- 53 View
- 0 Download
- 10 Crossref
-
Abstract
- Ferric uptake regulator (Fur) is a global regulator involved in multiple aspects of bacterial life. The gene encoding the Vibrio harveyi Fur (FurVh) was cloned from a pathogenic V. harveyi strain isolated from diseased fish. FurVh shares 77% overall sequence identity with the Escherichia coli Fur (FurEc) and could complement a mutant of FurEc. Like FurEc, FurVh possesses two cysteine residues at positions 92 and 95, yet unlike FurEc, in which these cysteine residues constitute part of the metal ion coordination site and hence are vital to the repressor activity, C92 and C95 of FurVh proved to be functionally inessential. Further study identified a Vibrio Fur signature sequence, which is preserved in all the ten Vibrio Fur proteins that have been discovered to date but in none of the non-vibrio Fur proteins. Site-directed and random mutation analyses of the signature residues, the cysteine residues, and seven highly charged amino acid residues indicated that D9, H32, C137, and K138 of FurVh are functionally important but D9, C137, and K138 can be replaced by more than one functional substitutes. Systematic deletion analysis demonstrated that the C-terminal 12 residues of FurVh are functionally inessential. These results (i) indicated that the activation mechanism, or certain aspects of which, of FurVh is possibly different from that of FurEc; and (ii) suggested that it is not very likely that the C-terminal 12 residues play any significant role in the activation or stability of FurVh; and (iii) provided insights into the potential function of the local structure involving C137 and K138.
-
Citations
Citations to this article as recorded by
- Comparative analysis of the survival and gene expression of pathogenic strainsVibrio harveyiafter starvation
Jingjing Sun, Xiaojian Gao, Jiang Qun, Xuedi Du, Keran Bi, Xiaojun Zhang, Li Lin, Alejandra Bravo
FEMS Microbiology Letters.2016; 363(22): fnw250. CrossRef - The FUR (ferric uptake regulator) superfamily: Diversity and versatility of key transcriptional regulators
María F. Fillat
Archives of Biochemistry and Biophysics.2014; 546: 41. CrossRef - DNA adenine methylase is involved in the pathogenesis of Edwardsiella tarda
Kun Sun, Xu-dong Jiao, Min Zhang, Li Sun
Veterinary Microbiology.2010; 141(1-2): 149. CrossRef - Experimental and computational characterization of the ferric uptake regulator from Aliivibrio salmonicida (Vibrio salmonicida)
Hege Lynum Pedersen, Rafi Ahmad, Ellen Kristin Riise, Hanna-Kirsti Schrøder Leiros, Stefan Hauglid, Sigrun Espelid, Bjøn Olav Brandsdal, Ingar Leiros, Nils-Peder Willassen, Peik Haugen
The Journal of Microbiology.2010; 48(2): 174. CrossRef - Helicobacter pylori apo-Fur regulation appears unconserved across species
Shana Miles, Beth M. Carpenter, Hanan Gancz, D. Scott Merrell
The Journal of Microbiology.2010; 48(3): 378. CrossRef - Construction of an attenuated Pseudomonas fluorescens strain and evaluation of its potential as a cross-protective vaccine
Huan-ran Wang, Yong-hua Hu, Wei-wei Zhang, Li Sun
Vaccine.2009; 27(30): 4047. CrossRef - A ZnS4 Structural Zinc Site in the Helicobacter pylori Ferric Uptake Regulator
Sylvia Vitale, Caroline Fauquant, David Lascoux, Kristine Schauer, Christine Saint-Pierre, Isabelle Michaud-Soret
Biochemistry.2009; 48(24): 5582. CrossRef - Crystal structure of the Vibrio cholerae ferric uptake regulator (Fur) reveals insights into metal co‐ordination
Md. Arif Sheikh, Garry L. Taylor
Molecular Microbiology.2009; 72(5): 1208. CrossRef - Attenuation ofEdwardsiella tardaVirulence by Small Peptides That Interfere with LuxS/Autoinducer Type 2 Quorum Sensing
Min Zhang, Xu-dong Jiao, Yong-hua Hu, Li Sun
Applied and Environmental Microbiology.2009; 75(12): 3882. CrossRef - Domain analysis of the Edwardsiella tarda ferric uptake regulator
Kun Sun, Shuang Cheng, Fang Wang, Li Sun
The Journal of General and Applied Microbiology.2009; 55(5): 351. CrossRef
- Comparative analysis of the survival and gene expression of pathogenic strainsVibrio harveyiafter starvation
Journal Article
- NOTE] Bioluminescent Assay for Sphingolipid Ceramide N-Deacylase Using Vibrio harveyi Dark Mutant M-17
- Ki Woong Cho
- J. Microbiol. 2008;46(5):585-589. Published online October 31, 2008
- DOI: https://doi.org/10.1007/s12275-008-0114-2
- 39 View
- 0 Download
- 1 Scopus
-
Abstract
- A new bioluminescent assay method for the activity of sphingolipid ceramide N-deacylase (SCDase: EC3.5.1.69) as well as ceramidase (CDase: EC 3.5.1.23) was developed using bioluminescent marine bacteria. Enzymatically synthesized ceramide (N-myristoyl sphigosine, C14:0-18:1) and commercial SCDase were used in this demonstration, and myristic (tetradecanoic, C14:0) acid produced by the SCDase hydrolysis was quantified using Vibrio harveyi M-17, a dark mutant of V. harveyi. The in vivo light intensity of M-17 was stimulated up to thousands fold in the presence of myristic acid, was used for this assay. SCDase activity with as little as 10 μU and 5 nM of myristic acid production were detected in less than one min. <br>The assay worked well for the determination of Km and chromatographic fraction assay.
First
Prev
TOP
