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- Raman spectroscopy reveals alteration of spore compositions under different nutritional conditions in Lysinibacillus boronitolerans YS11
- Youngung Ryu , Minyoung Hong , Soo Bin Kim , Tae Kwon Lee , Woojun Park
- J. Microbiol. 2021;59(5):491-499. Published online March 29, 2021
- DOI: https://doi.org/10.1007/s12275-021-0679-6
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- 7 Crossref
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Abstract
- Little is known about final spores components when bacteria undergo sporulation under different nutrient conditions. Different degrees of resistance and germination rates were observed in the three types of spores of Lysinibacillus boronitolerans YS11 (SD, Spores formed in Difco sporulation mediumTM; SC and SF, Spores formed in an agricultural byproduct medium with 10 mM CaCl2 and with 10 mM FeSO4, respectively). Stronger UV resistance was recorded for SF with 1.8–2.3-fold greater survival than SC and SD under UV treatment. The three spore types showed similar heat resistances at 80°C, but survival rates of SC and SD were much higher (~1,000 times) than those of SF at 90°C. However, germination capacity of SF was 20% higher than those of SD and SC on Luria-Bertani agar plates for 24 h. SF germinated more rapidly in a liquid medium with high NaCl concentrations than SC and SD, but became slower under alkaline conditions. Raman spectroscopy was used to analyze the heterogeneities in the three types of vegetative cells and their spores under different nutritional conditions. Exponentially grown-each vegetative cells had different overall Raman peak values. Raman peaks of SC, SD, and SF also showed differences in adenine and amide III compositions and nucleic acid contents. Our data along with Raman spectroscopy provided the evidence that spores formed under under different growth conditions possess very different cellular components, which affected their survival and germination rates.
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Citations
Citations to this article as recorded by
- Characterization of the Bacillus cereus spore killed by plasma-activated water (PAW)
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Food Research International.2024; 196: 115058. CrossRef - Alleviation of H2O2 toxicity by extracellular catalases in the phycosphere of Microcystis aeruginosa
Yerim Park, Wonjae Kim, Yeji Cha, Minkyung Kim, Woojun Park
Harmful Algae.2024; 137: 102680. CrossRef - Effects of sporulation conditions on the growth, germination, and resistance of Clostridium perfringens spores
Dong Liang, Xiaoshuang Cui, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Shijie Liu, Gaiming Zhao, Na Wang, Yangyang Ma, Lina Xu
International Journal of Food Microbiology.2023; 396: 110200. CrossRef - Lysinibacilli: A Biological Factories Intended for Bio-Insecticidal, Bio-Control, and Bioremediation Activities
Qazi Mohammad Sajid Jamal, Varish Ahmad
Journal of Fungi.2022; 8(12): 1288. CrossRef - Discrimination of Stressed and Non-Stressed Food-Related Bacteria Using Raman-Microspectroscopy
Daniel Klein, René Breuch, Jessica Reinmüller, Carsten Engelhard, Peter Kaul
Foods.2022; 11(10): 1506. CrossRef - Detection of low numbers of bacterial cells in a pharmaceutical drug product using Raman spectroscopy and PLS-DA multivariate analysis
R. A. Grosso, A. R. Walther, E. Brunbech, A. Sørensen, B. Schebye, K. E. Olsen, H. Qu, M. A. B. Hedegaard, E. C. Arnspang
The Analyst.2022; 147(15): 3593. CrossRef - Linkage between bacterial community-mediated hydrogen peroxide detoxification and the growth of Microcystis aeruginosa
Minkyung Kim, Wonjae Kim, Yunho Lee, Woojun Park
Water Research.2021; 207: 117784. CrossRef
- Characterization of the Bacillus cereus spore killed by plasma-activated water (PAW)
Research Support, Non-U.S. Gov't
- Occurrence of the strA-strB Streptomycin Resistance Genes in Pseudomonas Species Isolated from Kiwifruit Plants
- Hyo Shim Han , Young Jin Koh , Jae-Seoun Hur , Jae Sung Jung
- J. Microbiol. 2004;42(4):365-368.
- DOI: https://doi.org/2096 [pii]
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Abstract
- The occurrence of strA-strB streptomycin-resistance genes within transposon Tn5393 was examined in Pseudomonas syringae pv. actinidiae, P. syringae pv. syringae, and P. marginalis, isolated from kiwifruit plants in Korea and Japan. PCR amplification with primers specific to strA-strB revealed that three of the tested Pseudomonas species harbored these genes for a streptomycin-resistance determinant. Tn5393, containing strA-strB, was also identified with PCR primers designed to amplify parts of tnpA, res, and tnpR. No IS elements were detected within tnpR, nor were they found in the intergenic region between tnpR and strA. Nucleotide sequence analysis indicated that the strA sequence of P. syringae pv. actinidiae contained a single nucleotide alteration at position 593 (CAA→CGA), as compared to Tn5393a in P. syringae pv. syringae. This resulted in an amino acid change, from Gln to Arg.
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