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- Reducing the Bioactivity of Tannerella forsythia Lipopolysaccharide by Porphyromonas gingivalis
- Young-Jae Kim , Sung-Hoon Lee
- J. Microbiol. 2014;52(8):702-708. Published online August 1, 2014
- DOI: https://doi.org/10.1007/s12275-014-4324-5
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- 13 Crossref
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Abstract
- Tannerella forsythia is considered a pathogen of periodontitis and forms a biofilm with multi-species bacteria in oral cavity. Lipopolysaccharide is a powerful immunostimulator and induces inflammation and shock. The purpose of this study was to investigate the characteristics of T. forsythia LPS in its co-cultivation with Fusobacterium nucleatum or Porphyromonas gingivalis. T. forsythia was co-cultured in the presence and absence of F. nucleatum and P. gingivalis and then T. forsythia LPS was extracted. The extracts were analyzed by SDS-PAGE and NF-κB reporter CHO cell lines. THP-1 cells were treated with the LPS and evaluated induction of cytokine expression by real-time RT-PCR and ELISA. For analysis of the bioactivity of T. forsythia LPS, the binding assay on LPS-binding protein (LBP) and CD14 was processed. The extracts did not contaminate other molecules except LPS and showed TLR4 agonists. Co-cultured T. forsythia LPS with P. gingivalis exhibited a lower level of induction of TNF-α, IL-1β, and IL-6 expression than singleor co-cultured T. forsythia LPS with F. nucleatum in the conditions of human serum. However, the three T. forsythia LPS did not show difference of cytokine induction in the serum free conditions. Co-cultured T. forsythia LPS with P. gingivalis exhibited a lower affinity to LBP and CD14 as binding site of O-antigen and attached at a lower level to THP-1 cells compared to single- or co-cultured T. forsythia LPS with F. nucleatum. The virulence of T. forsythia LPS was decreased by co-culturing with P. gingivalis and their affinity to LBP and CD14 was reduced, which may due to modification of O-antigen chain by P. gingivalis.
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Citations
Citations to this article as recorded by
- Anti-Inflammatory Efficacy of Human-Derived Streptococcus salivarius on Periodontopathogen-Induced Inflammation
Dong-Heon Baek, Sung-Hoon Lee
Journal of Microbiology and Biotechnology.2023; 33(8): 998. CrossRef - Virulence of Filifactor alocis lipoteichoic acid on human gingival fibroblast
Hyun-Jun Yoo, Sung-Hoon Lee
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Frontiers in Microbiology.2017;[Epub] CrossRef - Gingipain‐dependent augmentation by Porphyromonas gingivalis of phagocytosis of Tannerella forsythia
Y.‐J. Jung, H.‐K. Jun, B.‐K. Choi
Molecular Oral Microbiology.2016; 31(6): 457. CrossRef - Development of Filifactor alocis media for single- and co-cultivation with periodontopathogens
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Oral Biology Research.2016; 40(4): 193. CrossRef - Antagonistic effect of peptidoglycan of Streptococcus sanguinis on lipopolysaccharide of major periodontal pathogens
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Journal of Microbiology.2015; 53(8): 553. CrossRef - Polymicrobial Oral Infection with Four Periodontal Bacteria Orchestrates a Distinct Inflammatory Response and Atherosclerosis in ApoEnull Mice
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PLOS ONE.2015; 10(11): e0143291. CrossRef
- Anti-Inflammatory Efficacy of Human-Derived Streptococcus salivarius on Periodontopathogen-Induced Inflammation
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