Journal of Microbiology

Journal Articles

Salmonella Typhimurium ST313 isolated in Brazil revealed to be more invasive and inflammatory in murine colon compared to ST19 strains: Amanda Aparecida Seribelli , Tamara R. Machado Ribeiro , Patrick da Silva† , Isabela Mancini Martins , Felipe Pinheiro Vilela , Marta I. Cazentini Medeiros , Kamila Chagas Peronni , Wilson Araújo da Silva Junior , Cristiano Gallina Moreira , Juliana Pfrimer Falcão; J. Microbiol. 2021;59(9):861-870. Published online August 12, 2021; DOI: https://doi.org/10.1007/s12275-021-1082-z

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Salmonella Typhimurium (ST313) has caused an epidemic of invasive disease in sub-Saharan Africa and has been recently identified in Brazil. As the virulence of this ST is poorly understood, the present study aimed to (i) perform the RNAseq in vitro of S. Typhimurium STm30 (ST313) grown in Luria-Bertani medium at 37°C; (ii) compare it with the RNAseq of the S. Typhimurium SL1344 (ST19) and S. Typhimurium STm11 (ST19) strains under the same growing conditions; and (iii) examine the colonization capacity and expression of virulence genes and cytokines in murine colon. The STm30 (ST313) strain exhibited stronger virulence and was associated with a more inflammatory profile than the strains SL1344 (ST19) and STm11 (ST19), as demonstrated by transcriptome and in vivo assay. The expression levels of the hilA, sopD2, pipB, and ssaS virulence genes, other Salmonella pathogenicity islands SPI-1 and SPI-2 genes or effectors, and genes of the cytokines IL-1β, IFN-γ, TNF-α, IL-6, IL-17, IL-22, and IL-12 were increased during ST313 infection in C57BL/6J mice. In conclusion, S. Typhimurium STm30 (ST313) isolated from human feces in Brazil express higher levels of pathogenesis- related genes at 37°C and has stronger colonization and invasion capacity in murine colon due to its high expression levels of virulence genes, when compared with the S. Typhimurium SL1344 (ST19) and STm11 (ST19) strains. STm30 (ST313) also induces stronger expression of pro-inflammatory cytokines in this organ, suggesting that it causes more extensive tissue damage.

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Whole-Genome Sequencing Reveals the Population Structure and Genetic Diversity of Salmonella Typhimurium ST34 and ST19 Lineages
Zhen-xu Zhuo, Yu-lian Feng, Xi-wei Zhang, Hao Liu, Fang-yin Zeng, Xiao-yan Li
Journal of Microbiology.2024; 62(10): 859. CrossRef
Incremental increases in physiological fluid shear progressively alter pathogenic phenotypes and gene expression in multidrug resistant Salmonella
Jiseon Yang, Jennifer Barrila, Eric A. Nauman, Seth D. Nydam, Shanshan Yang, Jin Park, Ami D. Gutierrez-Jensen, Christian L. Castro, C. Mark Ott, Kristina Buss, Jason Steel, Anne D. Zakrajsek, Mary M. Schuff, Cheryl A. Nickerson
Gut Microbes.2024;[Epub] CrossRef
Virulence potential of Salmonella 1,4, [5],12:i:- strains isolated during decades from different sources in the Southeast region of Brazil
Giovana do Nascimento Pereira, Amanda Aparecida Seribelli, Carolina Nogueira Gomes, Felipe Pinheiro Vilela, Ludmilla Tonani, Monique Ribeiro Tiba-Casas, Marta Inês Cazentini Medeiros, Dália dos Prazeres Rodrigues, Márcia Regina von Zeska Kress, Juliana Pf
Brazilian Journal of Microbiology.2023; 54(4): 2827. CrossRef
Invasive non-typhoidal Salmonella (iNTS) aminoglycoside-resistant ST313 isolates feature unique pathogenic mechanisms to reach the bloodstream
Isabela Mancini Martins, Amanda Aparecida Seribelli, Tamara R. Machado Ribeiro, Patrick da Silva, Bruna Cardinali Lustri, Rodrigo T. Hernandes, Juliana Pfrimer Falcão, Cristiano Gallina Moreira
Infection, Genetics and Evolution.2023; 116: 105519. CrossRef
Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Microbial Pathogenesis.2022; 165: 105460. CrossRef
Antimicrobial resistance and genetic background of non-typhoidal Salmonella enterica strains isolated from human infections in São Paulo, Brazil (2000–2019)
Aline Parolin Calarga, Marco Tulio Pardini Gontijo, Luiz Gonzaga Paula de Almeida, Ana Tereza Ribeiro de Vasconcelos, Leandro Costa Nascimento, Taíse Marongio Cotrim de Moraes Barbosa, Thalita Mara de Carvalho Perri, Silvia Regina dos Santos, Monique Ribe
Brazilian Journal of Microbiology.2022; 53(3): 1249. CrossRef

Performance comparison of fecal preservative and stock solutions for gut microbiome storage at room temperature: Chanhyeok Park , Kyeong Eui Yun , Jeong Min Chu , Ji Yeon Lee , Chang Pyo Hong , Young Do Nam , Jinuk Jeong , Kyudong Han , Yong Ju Ahn; J. Microbiol. 2020;58(8):703-710. Published online June 25, 2020; DOI: https://doi.org/10.1007/s12275-020-0092-6

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Abstract

The gut microbiome, which is symbiotic within the human body, assists in human digestion. It plays significant roles in identifying intestinal disease as well as in maintaining a healthy body with functional immune and metabolic activities. To confirm the consistency of fecal intestinal microbial research, it is necessary to study the changes in intestinal microbial flora according to the fecal collection solution and storage period. We collected fecal samples from three healthy Korean adults. To examine the efficacy of fecal collection solution, we used NBgene-Gut, OMNIgene-Gut, 70% ethanol (Ethanol-70%), and RNAlater. The samples were stored for up to two months at room temperature using three different
methods
, and we observed changes in microbial communities over time. We analyzed clusters of changes in the microbial flora by observing fecal stock solutions and metagenome sequencing performed over time. In particular, we confirmed the profiling of alpha and beta diversity and microbial classification according to the differences in intestinal environment among individuals. We also confirmed that the microbial profile remained stable for two months and that the microbial profile did not change significantly over time. In addition, our results suggest the possibility of verifying microbial profiling even for long-term storage of a single sample. In conclusion, collecting fecal samples using a stock solution rather than freezing feces seems to be relatively reproducible and stable for GUT metagenome analysis. Therefore, stock solution tubes in intestinal microbial research can be used without problems.

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Sirui Zhou, Weijian Zhu, Hehua Guo, Yalan Nie, Jiazheng Sun, Ping Liu, Yulan Zeng
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Importance of Human Faecal Biobanking: From Collection to Storage
Izabela Lewandowska, Katarzyna Grzech, Jolanta Krzysztoń-Russjan
Advancements of Microbiology.2024; 63(4): 181. CrossRef
Effects of Stool Sample Preservation Methods on Gut Microbiota Biodiversity: New Original Data and Systematic Review with Meta-Analysis
Xin-meng Li, Xiao Shi, Yao Yao, Yi-cun Shen, Xiang-ling Wu, Ting Cai, Lun-xi Liang, Fen Wang, Po-Yu Liu
Microbiology Spectrum.2023;[Epub] CrossRef
Microbial Composition and Stool Short Chain Fatty Acid Levels in Fibromyalgia
Yunkyung Kim, Geun-Tae Kim, Jihun Kang
International Journal of Environmental Research and Public Health.2023; 20(4): 3183. CrossRef
Best practice for wildlife gut microbiome research: A comprehensive review of methodology for 16S rRNA gene investigations
Leigh Combrink, Ian R. Humphreys, Quinn Washburn, Holly K. Arnold, Keaton Stagaman, Kristin D. Kasschau, Anna E. Jolles, Brianna R. Beechler, Thomas J. Sharpton
Frontiers in Microbiology.2023;[Epub] CrossRef
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Taobing Yu, Lang Cheng, Qi Liu, Shasha Wang, Yuan Zhou, Hongbin Zhong, Meifang Tang, Hai Nian, Tengxiang Lian, Cheng Gao, Glade Dlott
Microbiology Spectrum.2022;[Epub] CrossRef
Effects of Stool Sample Preservation Methods on Gut Microbiota Biodiversity: New Original Data and Systematic Review with Meta-Analysis
Xin-meng Li, Xiao Shi, Yao Yao, Yi-cun Shen, Xiang-ling Wu, Fen Wang
SSRN Electronic Journal .2022;[Epub] CrossRef
Trialling a microbiome-targeted dietary intervention in children with ADHD—the rationale and a non-randomised feasibility study
Kate Lawrence, Kyriaki Myrissa, Miguel Toribio-Mateas, Lori Minini, Alice M. Gregory
Pilot and Feasibility Studies.2022;[Epub] CrossRef
Can the genetic variability of Blastocystis sp. be associated with the climatic region of its human carriers?
B. Ake-Canche, E. Rodriguez-Bataz, J.Y Esquivel-Piña, A. Tolentino-Loreto, S. Arroyo-Escalante, J. Martínez-Ocaña, M. Romero-Valdovinos, O. Valenzuela, G.E. Orozco-Mosqueda, F. Martinez-Hernandez, P. Maravilla, A. Martinez
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Critical evaluation of faecal microbiome preservation using metagenomic analysis
Alena L Pribyl, Donovan H Parks, Nicola Z Angel, Joel A Boyd, Alexander G Hasson, Liang Fang, Samantha L MacDonald, Blake A Wills, David L A Wood, Lutz Krause, Gene W Tyson, Philip Hugenholtz
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The effect of taxonomic classification by full-length 16S rRNA sequencing with a synthetic long-read technology
Jinuk Jeong, Kyeongeui Yun, Seyoung Mun, Won-Hyong Chung, Song-Yi Choi, Young-do Nam, Mi Young Lim, Chang Pyo Hong, ChanHyeok Park, Yong Ju Ahn, Kyudong Han
Scientific Reports.2021;[Epub] CrossRef
Dietary fiber and probiotics influence the gut microbiome and melanoma immunotherapy response
Christine N. Spencer, Jennifer L. McQuade, Vancheswaran Gopalakrishnan, John A. McCulloch, Marie Vetizou, Alexandria P. Cogdill, Md A. Wadud Khan, Xiaotao Zhang, Michael G. White, Christine B. Peterson, Matthew C. Wong, Golnaz Morad, Theresa Rodgers, Jona
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Impact of Plant-Based Meat Alternatives on the Gut Microbiota of Consumers: A Real-World Study
Miguel A. Toribio-Mateas, Adri Bester, Natalia Klimenko
Foods.2021; 10(9): 2040. CrossRef

Review

REVIEW] Type 3 regulatory T cells at the interface of symbiosis: Joo-Hong Park , Gérard Eberl; J. Microbiol. 2018;56(3):163-171. Published online February 28, 2018; DOI: https://doi.org/10.1007/s12275-018-7565-x

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Abstract

The mammalian gastrointestinal tract accommodates trillions of bacteria, many of which provide beneficial effects to the host, including protection from pathogenic microorganisms and essential metabolites. However, the intestinal immune system needs to adapt to the constantly fluctuating microbial environment at mucosal surfaces in order to maintain homeostasis. In particular, the gut microbiota induces the differentiation of effector Th17 cells and regulatory T cells (Tregs) that express RORγt, the master regulator of antimicrobial type 3 immunity. RORγt+ Tregs constitute a major population of colonic Tregs that is distinct from thymusderived Tregs and require bacterial antigens for differentiation. The balance between Th17 cells and RORγt+ Tregs, that is, the tone of the local type 3 immune response, is regulated by the vitamin A metabolite retinoic acid produced by the host. Furthermore, Th17 cells and RORγt+ Tregs regulate intestinal type 2 immune responses, explaining how bacteria block allergic reactions. Here, we review the cellular and molecular mechanisms involved in the differentiation, regulation and function of RORγt+ (type 3) Tregs, and discuss the multiple equilibria that exist between effector T cells and Tregs, as well as between different types of immune responses, which are necessary to maintain homeostasis and health.

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World Journal of Gastroenterology.2024; 30(8): 833. CrossRef
Endotoxin Inflammatory Action on Cells by Dysregulated-Immunological-Barrier-Linked ROS-Apoptosis Mechanisms in Gut–Liver Axis
Andrei Dumitru, Elena Matei, Georgeta Camelia Cozaru, Anca Chisoi, Luana Alexandrescu, Răzvan Cătălin Popescu, Mihaela Pundiche Butcaru, Eugen Dumitru, Sorin Rugină, Cristina Tocia
International Journal of Molecular Sciences.2024; 25(5): 2472. CrossRef
MAFLD and Celiac Disease in Children
Serena Scapaticci, Annamaria Venanzi, Francesco Chiarelli, Cosimo Giannini
International Journal of Molecular Sciences.2023; 24(2): 1764. CrossRef
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Aleksi Husso, Tiina Pessa-Morikawa, Ville Mikael Koistinen, Olli Kärkkäinen, Hyuk Nam Kwon, Leo Lahti, Antti Iivanainen, Kati Hanhineva, Mikael Niku
BMC Biology.2023;[Epub] CrossRef
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Tejinder Pal Singh, Saurabh Kadyan, Harisha Devi, Gwoncheol Park, Ravinder Nagpal
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Pathogens.2023; 12(9): 1087. CrossRef
The role of microbiota in nonalcoholic fatty liver disease
Agostino Di Ciaula, Leonilde Bonfrate, Piero Portincasa
European Journal of Clinical Investigation.2022;[Epub] CrossRef
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Piero Portincasa, Leonilde Bonfrate, Mohamad Khalil, Maria De Angelis, Francesco Maria Calabrese, Mauro D’Amato, David Q.-H. Wang, Agostino Di Ciaula
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Journal Articles

Recombinant baculovirus-based vaccine expressing M2 protein induces protective CD8+ T-cell immunity against respiratory syncytial virus infection: Jeong-Yoon Lee , Jun Chang; J. Microbiol. 2017;55(11):900-908. Published online October 27, 2017; DOI: https://doi.org/10.1007/s12275-017-7306-6

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Abstract

Respiratory syncytial virus (RSV) is an important cause of acute lower respiratory tract disease in infants, young children, immunocompromised individuals, and the elderly. However, despite ongoing efforts to develop an RSV vaccine, there is still no authorized RSV vaccine for humans. Baculovirus has attracted attention as a vaccine vector because of its ability to induce a high level of humoral and cellular immunity, low cytotoxicity against various antigens, and biological safety for humans. In this study, we constructed a recombinant baculovirus- based vaccine expressing the M2 protein of RSV under the control of cytomegalovirus promoter (Bac_RSVM2) to induce CD8+ T-cell responses which play an important role in viral clearance, and investigated its protective efficacy against RSV infection. Immunization with Bac_RSVM2 via intranasal or intramuscular route effectively elicited the specific CD8+ T-cell responses. Most notably, immunization with Bac_RSVM2 vaccine almost completely protected mice from RSV challenge without vaccine-enhanced immunopathology. In conclusion, these results suggest that Bac_RSVM2 vaccine employing the baculovirus delivery platform has promising potential to be developed as a safe and novel RSV vaccine that provides protection against RSV infection.

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Respiratory delivered vaccines: Current status and perspectives in rational formulation design
Lan Wu, Wenwen Xu, Huiyang Jiang, Mingshi Yang, Dongmei Cun
Acta Pharmaceutica Sinica B.2024; 14(12): 5132. CrossRef
Enhanced virulence of genetically engineered Autographa californica nucleopolyhedrovirus owing to accelerated viral DNA replication aided by inserted ascovirus genes
Huan Yu, Chang-Jin Yang, Yi-Yi Ou-Yang, Yue Tong, Hui-Yu Lan, Jia-Min Gan, Shi-Wei Li, Ding-Yi Bai, Guo-Hua Huang
Pesticide Biochemistry and Physiology.2023; 192: 105382. CrossRef
Cytokines and CD8 T cell immunity during respiratory syncytial virus infection
Megan E. Schmidt, Steven M. Varga
Cytokine.2020; 133: 154481. CrossRef
Induction of mucosal immunity against pathogens by using recombinant baculoviral vectors: Mechanisms, advantages, and limitations
Mario Fragoso-Saavedra, Marco A Vega-López
Journal of Leukocyte Biology.2020; 108(3): 835. CrossRef
Endogenous n-3 Polyunsaturated Fatty Acids Are Beneficial to Dampen CD8+ T Cell-Mediated Inflammatory Response upon the Viral Infection in Mice
Kyung Won Kang, Seyoung Kim, Yong-Bin Cho, Seung Rok Ryu, Young-Jin Seo, Sang-Myeong Lee
International Journal of Molecular Sciences.2019; 20(18): 4510. CrossRef
Anti-viral activity of compounds from Agrimonia pilosa and Galla rhois extract mixture
Jeong Eun Kwon, Yeong-Geun Lee, Ji-Hun Kang, Yun-Feng Bai, Yong Joon Jeong, Nam-In Baek, Young-Jin Seo, Se Chan Kang
Bioorganic Chemistry.2019; 93: 103320. CrossRef
Vaccine containing G protein fragment and recombinant baculovirus expressing M2 protein induces protective immunity to respiratory syncytial virus
Yeong-Min Jo, Jungwoo Kim, Jun Chang
Clinical and Experimental Vaccine Research.2019; 8(1): 43. CrossRef
Recombinant live attenuated influenza vaccine viruses carrying CD8 T-cell epitopes of respiratory syncytial virus protect mice against both pathogens without inflammatory disease
Tatiana Kotomina, Irina Isakova-Sivak, Victoria Matyushenko, Ki-Hye Kim, Youri Lee, Yu-Jin Jung, Sang-Moo Kang, Larisa Rudenko
Antiviral Research.2019; 168: 9. CrossRef
The CD8 T Cell Response to Respiratory Virus Infections
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A rapid and simple method for identifying bacterial polar lipid components in wet biomass: Tuan Manh Nguyen , Jaisoo Kim; J. Microbiol. 2017;55(8):635-639. Published online July 4, 2017; DOI: https://doi.org/10.1007/s12275-017-7092-1

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Abstract

There are marked differences between wet and freeze-dried cells with regard to the identification of polar lipid components. The determination of the polar lipid composition of freeze-dried cells is well established. However, several approaches to identifying polar lipid components in wet cells have met with limited success owing to the presence of non-polar compounds in the extracts, resulting in a lipid composition with a narrow scope. In this study, we surveyed the lipid profiles of the wet biomasses of three Gram-positive (Microbacterium lacticum, Rhodococcus koreensis, and Streptomyces longwoodensis) and two Gram-negative (Pseudomonas aeruginosa and Novosphingobium capsulatum) bacteria; the results were comparable in quality to those obtained using a standard freeze-dried approach. Moreover, our improved method ensures simple lipid extraction. Overall, the results of the analysis showed minor lipid profile differences between the two approaches with regard to quantity, and lipid identification was consistent in both methods for all species.

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Review

REVIEW] Modulation of the host immune response by respiratory syncytial virus proteins: Megan E. Schmidt , Steven M. Varga; J. Microbiol. 2017;55(3):161-171. Published online February 28, 2017; DOI: https://doi.org/10.1007/s12275-017-7045-8

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Abstract

Respiratory syncytial virus (RSV) causes severe respiratory disease in both the very young and the elderly. Nearly all individuals become infected in early childhood, and reinfections with the virus are common throughout life. Despite its clinical impact, there remains no licensed RSV vaccine. RSV infection in the respiratory tract induces an inflammatory response by the host to facilitate efficient clearance of the virus. However, the host immune response also contributes to the respiratory disease observed following an RSV infection. RSV has evolved several mechanisms to evade the host immune response and promote virus replication through interactions between RSV proteins and immune components. In contrast, some RSV proteins also play critical roles in activating, rather than suppressing, host immunity. In this review, we discuss the interactions between individual RSV proteins and host factors that modulate the immune response and the implications of these interactions for the course of an RSV infection.

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The effect of dietary bovine colostrum on respiratory syncytial virus infection and immune responses following the infection in the mouse: Mei Ling Xu , Hyoung Jin Kim , Ga Ram Wi , Hong-Jin Kim; J. Microbiol. 2015;53(9):661-666. Published online August 27, 2015; DOI: https://doi.org/10.1007/s12275-015-5353-4

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Abstract

Human respiratory syncytial virus (hRSV) is the most common cause of respiratory tract infection among young children because of immature T cell immunity of them against hRSV. CD8 T cells play a pivotal role in clearing hRSV and preventing subsequent infection. We examined the effects of dietary bovine colostrum on virus infection and CD8 T cell responses following hRSV infection in the mouse model. Mice received bovine colostrum for 14 days prior to hRSV challenge, and lung indexes (severity of symptom) and lung virus titers were analyzed. In addition, the activation of CD8 T cells in the bronchoalveolar lavage fluids (BALFs) of mice receiving bovine colostrum were compared with those in the BALFs of mice receiving phosphate-buffered saline (PBS) or ribavirin, post virus challenge. The severity of infection and lung virus titers were reduced in the mice receiving bovine colostrum, compared to those receiving PBS. Moreover CD8 T cell responses were selectively enhanced in the former. Our results suggest that dietary bovine colostrum exerts the effects to inhibit hRSV and ameliorate the symptom by hRSV infection, and enhances the CD8 T cell response during the hRSV infection.

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Colostrum supplement, immune variables and hospital-acquired infection in acute respiratory failure: A double-blind, randomized, placebo-controlled study
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Intestinal Intraepithelial TCRγδ+ T Cells are Activated by Normal Commensal Bacteria: Sang Phil Jeong , Jung-Ah Kang , Sung-Gyoo Park; J. Microbiol. 2012;50(5):837-841. Published online November 4, 2012; DOI: https://doi.org/10.1007/s12275-012-2468-8

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Abstract: TCRγδ+ T cells play a critical role in protecting the intestinal mucosa against pathogenic infection. In the absence of infection, TCRγδ+ T cell activation must be continuously regulated by T regulatory cells (Treg) to prevent the development of colitis. However, the activation of intestinal TCRγδ+ T cells under normal conditions has not been clearly resolved. In order to determine TCRγδ+ T cell activation in vivo, we designed an NF-κB based reporter system. Using the recombinant lentiviral method, we delivered the NF-κB reporter to isolated TCRγδ+ T cells, which were then adoptively transferred into normal mice. Our data indicate that the NF-κB activation level in TCRγδ+ T cells is higher in the intestinal intraepithelial layer than in the lamina propria region. In addition, the surface expression level of lymphocyte activation marker CD69 in TCRγδ+ T cells is also higher in the intestinal intraepithelial layer and this activation was reduced by Sulfatrim treatment which removes of commensal bacteria. Collectively, our data indicate that the TCRγδ+ T cell population attached to the intestinal lumen is constitutively activated even by normal commensal bacteria.

Induction of IL-8 in Periodontal Ligament Cells by H2O2: Yang-Sin Lee , Eun Jung Bak , Minyoung Kim , Wonse Park , Jeong Taeg Seo , Yun-Jung Yoo; J. Microbiol. 2008;46(5):579-584. Published online October 31, 2008; DOI: https://doi.org/10.1007/s12275-008-0182-3

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Abstract: Periodontitis is an inflammatory disease caused by bacteria. In periodontitis, reactive oxygen species (ROS) are released from inflammatory cells in response to bacteria. Interleukin (IL)-8 is one of pro-inflammatory cytokines. To investigate the role of ROS in pathogenesis of periodontitis, we estimated the effect of H2O2, one of ROS, on the expression of IL-8 in human periodontal ligament (PDL) cells. PDL cells were treated with H2O2. IL-8 expression was determined by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The phosphorylation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (p38) and c-jun NH2-terminal kinase (JNK) was estimated by Western blotting. Treatment with H2O2 at concentration of up to 250 μM increased IL-8 mRNA expression and production in a concentration-dependent manner. However, treatment with 500 μM H2O2 did not increase IL-8 production. Catalase, an inhibitor of H2O2, down-regulated the production of IL-8 induced by H2O2. H2O2 increased the phosphorylation of ERK, p38, and JNK. Pretreatment with PD98059 (ERK inhibitor), SB203580 (p38 inhibitor), or SP600125 (JNK inhibitor) decreased the IL-8 production induced by H2O2. These results indicate that H2O2 acts as an inducer of IL-8 secretion via activation of ERK, p38, and JNK in PDL cells. H2O2 deposited in periodontal tissue during inflammation against bacteria may accelerate tissue destruction via induction of IL-8 in PDL cells.

Propagation of Bombyx mori Nucleopolyhedrovirus in Nonpermissive Insect Cell Lines: Soo-Dong Woo , Jong Yul Roh , Jae Young Choi , Byung Rae Jin; J. Microbiol. 2007;45(2):133-138.; DOI: https://doi.org/2522 [pii]

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Abstract: This study addresses the susceptibility of Spodoptera frugiperda (Sf9 and Sf21), Trichoplusia ni (Hi5), and S. exigua (Se301) cells to the Bombyx mori nucleopolyhedrovirus (BmNPV). Although these cells have classically been considered nonpermissive to BmNPV, the cytopathic effect, an increase in viral yield, and viral DNA synthesis by BmNPV were observed in Sf9, Sf21, and Hi5 cells, but not in Se301 cells. Very late gene expression by BmNPV in these cell lines was also detected via β-galactosidase expression under the control of the polyhedrin promoter. Sf9 cells were most susceptible to BmNPV in all respects, followed by Sf21 and Hi5 cells in decreasing order, while the Se301 cells evidenced no distinct viral replication. This particular difference in viral susceptibility in each of the cell lines can be utilized for our understanding of the mechanisms underlying the host specificity of NPVs.

Characterization of Cell Wall Proteins from the soo1-1/ret1-1 Mutant of Saccharomyces cerevisiae: Dong-Won Lee , Ki-Hyun Kim , Se-Chul Chun , Hee-Moon Park; J. Microbiol. 2002;40(3):219-223.

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Abstract: In order to investigate the function of Soo1p/[alpha]-COP during post-translational modification and intracellular transport of cell wall proteins in Saccharomyces cerevisiae, cell wall proteins from the soo1-1/ret1-1 mutant cells were analyzed. SDS-PAGE analysis of biotin labeled cell wall proteins suggested that the soo1-1 mutation impairs post-translational modification of cell wall proteins, such as N- and/or O-glycosylation. Analysis of cell wall proteins with antibodies against [beta]-1,3-glucan and [beta]-1,6-glucan revealed alteration of the linkage between cell wall proteins and [beta]-glucans in the soo1-1 mutant cells. Compositional sugar analysis of the cell wall proteins also suggested that the soo1-1 mutation impairs glycosylation of cell wall protein in the ER, which is crucial for the maintenance of cell wall integrity.

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