Journal of Microbiology

Journal Articles

Extracellular products-mediated interspecific interaction between Pseudomonas aeruginosa and Escherichia coli: Yang Yuan , Jing Li , Jiafu Lin , Wenjuan Pan , Yiwen Chu , Balakrishnan Prithiviraj , Yidong Guo , Xinrong Wang , Kelei Zhao; J. Microbiol. 2021;59(1):29-40. Published online December 23, 2020; DOI: https://doi.org/10.1007/s12275-021-0478-0

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The Gram-negative pathogen Pseudomonas aeruginosa adopts several elaborate strategies to colonize a wide range of natural or clinical niches and to overcome the neighboring bacterial competitors in polymicrobial communities. However, the relationship and interaction mechanism of P. aeruginosa with other bacterial pathogens remains largely unexplored. Here we explore the interaction dynamics of P. aeruginosa and Escherichia coli, which frequently coinfect the lungs of immunocompromised hosts, by using a series of on-plate proximity assays and RNA-sequencing. We show that the extracellular products of P. aeruginosa can inhibit the growth of neighboring E. coli and induce a large-scale of transcriptional reprogramming of E. coli, especially in terms of cellular respiration- related primary metabolisms and membrane components. In contrast, the presence of E. coli has no significant effect on the growth of P. aeruginosa in short-term culture, but causes a dysregulated expression of genes positively controlled by the quorum-sensing (QS) system of P. aeruginosa during subsequent pairwise culture. We further demonstrate that the divergent QS-regulation of P. aeruginosa may be related to the function of the transcriptional regulator PqsR, which can be enhanced by E. coli culture supernatant to increase the pyocyanin production by P. aeruginosa in the absence of the central las-QS system. Moreover, the extracellular products of E. coli promote the proliferation and lethality of P. aeruginosa in infecting the Caenorhabditis elegans model. The current study provides a general characterization of the extracellular products-mediated interactions between P. aeruginosa and E. coli, and may facilitate the understanding of polymicrobial infections.

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Pigments from pathogenic bacteria: a comprehensive update on recent advances
Kusumita Acharya, Swarna Shaw, Sudipta Paul Bhattacharya, Shatarupa Biswas, Suman Bhandary, Arijit Bhattacharya
World Journal of Microbiology and Biotechnology.2024;[Epub] CrossRef
Selective detection of two bacterial species in a single collision system targeting metabolic products
Jun Lin, Qingwen Wang, Huike Tian, Qing Xin, Dong Zhang
Microchemical Journal.2024; 206: 111572. CrossRef
Effect of the Type VI Secretion System Secreted Protein Hcp on the Virulence of Aeromonas salmonicida
Hongyan Cai, Jiaying Yu, Ying Qiao, Ying Ma, Jiang Zheng, Mao Lin, Qingpi Yan, Lixing Huang
Microorganisms.2022; 10(12): 2307. CrossRef

Description of a novel pectin-degrading bacterial species Prevotella pectinovora sp. nov., based on its phenotypic and genomic traits: Brigita Nograsek , Tomaz Accetto , Lijana Fanedl , Gorazd Avgustin; J. Microbiol. 2015;53(8):503-510. Published online July 31, 2015; DOI: https://doi.org/10.1007/s12275-015-5142-0

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Abstract

Five strictly anaerobic Gram-negative bacterial strains, P4-65, P4-76T, P5-60, P5-119, and P5-125, presumably belonging to the genus Prevotella were isolated from pig fecal samples. Strains were tested for various phenotypic traits and nearcomplete genome sequences were obtained and analyzed. Phylogenetic analysis based on 16S rRNA gene sequences and multilocus sequence analysis based on five conserved genes confirmed that the strains belong to the genus Prevotella, revealing that they represent a novel and discrete lineage distinct from other known species of this genus. The size of the genome of the isolated strains is 3?.3 Mbp, and the DNA G+C content is 47.5?8.1 mol%. The isolates are strictly anaerobic, rod-shaped with rounded ends, non-motile and non-spore-forming. The main fermentation products are succinate and acetate, with minor concentrations of isovalerate, propionate and isobutyrate. Hydrogen is also produced. Major cellular fatty acids consist of anteiso-C15:0 and iso-C15:0, and a number of additional acids are present in lower concentrations. A substantial portion of genes involved in carbohydrate utilization is devoted to pectin degradation and utilization, while those supporting growth on xylan in ruminal Prevotella could not have been revealed. On the basis of the presented results, a novel species, Prevotella pectinovora sp. nov. is proposed. The type strain is P4-76T (=DSM 29996T =ZIM B1020T).

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Discovery of two novel Flavobacterium species with potential for complex polysaccharide degradation
Xu-Dong Lian, Yong Guan, Yue Jiang, Dong-Heui Kwak, Mi-Kyung Lee, Zhun Li
Scientific Reports.2025;[Epub] CrossRef
Colonic Microbiota Improves Fiber Digestion Ability and Enhances Absorption of Short-Chain Fatty Acids in Local Pigs of Hainan
Pengxiang Xue, Mingming Xue, Yabiao Luo, Qiguo Tang, Feng Wang, Ruiping Sun, Yanxia Song, Zhe Chao, Meiying Fang
Microorganisms.2024; 12(6): 1033. CrossRef
Contribution of pectin-degrading bacteria to the quality of cigar fermentation: an analysis based on microbial communities and physicochemical components
Youbo Su, Yonghe Cui, Kejian Fu, Lingduo Bu, Yucui Sun, Qi Zhou, Yuming Yin, Yulong Sun, Huating Yang, Lang Wu, Xueru Song
Frontiers in Microbiology.2024;[Epub] CrossRef
Rumen fermentation of meal-fed sheep in response to diets formulated to vary in fiber and protein degradability
Sathya Sujani, Claire B Gleason, Barbara R dos Reis, Robin R White
Journal of Animal Science.2024;[Epub] CrossRef
Effects of supplementation of nonforage fiber source in diets with different starch levels on growth performance, rumen fermentation, nutrient digestion, and microbial flora of Hu lambs
Tongqing Guo, Zhi Lan Wang, Long Guo, Fadi Li, Fei Li
Translational Animal Science.2021;[Epub] CrossRef
Weaning Age and Its Effect on the Development of the Swine Gut Microbiome and Resistome
Devin B. Holman, Katherine E. Gzyl, Kathy T. Mou, Heather K. Allen, Paul D. Cotter
mSystems.2021;[Epub] CrossRef
Comparison of the composition and function of the gut microbiome in herdsmen from two pasture regions, Hongyuan and Xilingol
Chengcong Yang, Chuantao Peng, Hao Jin, Lijun You, Jiao Wang, Haiyan Xu, Zhihong Sun
Food Science & Nutrition.2021; 9(6): 3258. CrossRef
Prevotella in Pigs: The Positive and Negative Associations with Production and Health
Samat Amat, Hannah Lantz, Peris M. Munyaka, Benjamin P. Willing
Microorganisms.2020; 8(10): 1584. CrossRef
Cellulase and Alkaline Treatment Improve Intestinal Microbial Degradation of Recalcitrant Fibers of Rapeseed Meal in Pigs
Cheng Long, Christiane Rösch, Sonja de Vries, Henk Schols, Koen Venema
Journal of Agricultural and Food Chemistry.2020; 68(39): 11011. CrossRef
Dietary Lipids Influence Bioaccessibility of Polyphenols from Black Carrots and Affect Microbial Diversity under Simulated Gastrointestinal Digestion
Chunhe Gu, Hafiz A. R. Suleria, Frank R. Dunshea, Kate Howell
Antioxidants.2020; 9(8): 762. CrossRef
Effect of chemical oxygen demand load on the nitrification and microbial communities in activated sludge from an aerobic nitrifying reactor
Dan Li, Xihong Liang, Zhengwei Li, Yao Jin, Rongqing Zhou, Chongde Wu
Canadian Journal of Microbiology.2020; 66(1): 59. CrossRef
Changes of Microbial Diversity During Swine Manure Treatment Process
Minseok Kim, Jung-Im Yun, Seung-Gun Won, Kyu-Hyun Park
Polish Journal of Microbiology.2018; 67(1): 109. CrossRef
The response of soil bacterial communities to mining subsidence in the west China aeolian sand area
Peili Shi, Yuxiu Zhang, Zhenqi Hu, Kang Ma, Hao Wang, Tuanyao Chai
Applied Soil Ecology.2017; 121: 1. CrossRef
How to Feed the Mammalian Gut Microbiota: Bacterial and Metabolic Modulation by Dietary Fibers
Chiara Ferrario, Rosario Statello, Luca Carnevali, Leonardo Mancabelli, Christian Milani, Marta Mangifesta, Sabrina Duranti, Gabriele A. Lugli, Beatriz Jimenez, Samantha Lodge, Alice Viappiani, Giulia Alessandri, Margerita Dall’Asta, Daniele Del Rio, Andr
Frontiers in Microbiology.2017;[Epub] CrossRef

Research Support, Non-U.S. Gov't

In vitro effects of N-acetyl cysteine alone and in combination with antibiotics on Prevotella intermedia: Ji-Hoi Moon , Eun-Young Jang , Kyu Sang Shim , Jin-Yong Lee; J. Microbiol. 2015;53(5):321-329. Published online May 3, 2015; DOI: https://doi.org/10.1007/s12275-015-4500-2

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Abstract

N-acetyl cysteine (NAC) is an antioxidant that possesses anti-inflammatory activities in tissues. In the field of dentistry, NAC was demonstrated to prevent the expression of LPS-induced inflammatory mediators in phagocytic cells and gingival fibroblasts during the inflammatory process, but the effect of NAC on oral pathogens has been rarely studied. Here, we examined the effect of NAC against planktonic and biofilm cells of Prevotella intermedia, a major oral pathogen. NAC showed antibacterial activity against the planktonic P. intermedia with MIC value of 3 mg/ml and significantly decreased biofilm formation by the bacterium even at sub MIC. NAC did not affect the antibiotic susceptibility of planktonic P. intermedia, showing indifference (fractional inhibitory concentration index of 0.5?) results against the bacterium in combination with ampicillin, ciprofloxacin, tetracycline or metronidazole. On the other hand, viability of the pre-established bacterial biofilm exposed to the antibiotics except metronidazole was increased in the presence of NAC. Collectively, NAC may be used for prevention of the biofilm formation by P. intermedia rather than eradication of the pre-established bacterial biofilm. Further studies are required to explore antibacterial and anti-biofilm activity of NAC against mixed population of oral bacteria and its modulatory effect on antibiotics used for oral infectious diseases.

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Promoting Dentin Bridge Formation Through N-Acetyl-L-Cysteine Application in Rat Molar Pulpotomy: An Experimental Study
Kota Takagi, Koichi Nakamura, Yoshitaka Yoshimura, Yasutaka Yawaka
Journal of Functional Biomaterials.2025; 16(4): 117. CrossRef
THE ANTIMICROBIAL EFFECT OF N-ACETYLCYSTEINE AND ITS INTERACTION WITH ANTIBIOTICS AGAINST ACINETOBACTER BAUMANNII ISOLATES
Alparslan Semih SALAN, Suzan ÖKTEN
Ankara Universitesi Eczacilik Fakultesi Dergisi.2024; 48(2): 1. CrossRef
Intrinsic antimicrobial resistance: Molecular biomaterials to combat microbial biofilms and bacterial persisters
Swagatam Barman, Leman Buzoglu Kurnaz, Ryan Leighton, Md Waliullah Hossain, Alan W. Decho, Chuanbing Tang
Biomaterials.2024; 311: 122690. CrossRef
Exploring Immune Redox Modulation in Bacterial Infections: Insights into Thioredoxin-Mediated Interactions and Implications for Understanding Host–Pathogen Dynamics
Omer M. A. Dagah, Billton Bryson Silaa, Minghui Zhu, Qiu Pan, Linlin Qi, Xinyu Liu, Yuqi Liu, Wenjing Peng, Zakir Ullah, Appolonia F. Yudas, Amir Muhammad, Xianquan Zhang, Jun Lu
Antioxidants.2024; 13(5): 545. CrossRef
AADB: A Manually Collected Database for Combinations of Antibiotics With Adjuvants
Ji Lv, Guixia Liu, Yuan Ju, Houhou Huang, Ying Sun
IEEE/ACM Transactions on Computational Biology and Bioinformatics.2023; 20(5): 2827. CrossRef
Effect of N-Acetyl-L-cysteine on Activity of Doxycycline against Biofilm-Forming Bacterial Strains
Tsvetelina Petkova, Nikolina Rusenova, Svetla Danova, Aneliya Milanova
Antibiotics.2023; 12(7): 1187. CrossRef
Detection and quantification of pathogens in saliva of adolescents with cerebral palsy: a cross-sectional study
Rosemeire Arai Yoshida, Tiago Bertola Lobato, Renata Gorjão, Lucas Santiago França, Lívia Araujo Alves, Maria Teresa Botti Rodrigues Santos
Frontiers in Dental Medicine.2023;[Epub] CrossRef
The effect of biofilm inhibitor N-acetylcysteine on the minimum inhibitory concentration of antibiotics used in Gram-negative bacteria in the biofilm developed on catheters
Nilay Aksoy, Cansu Vatansever, Gizem Zengin Ersoy, Basak Adakli Aksoy, Tunç Fışgın
The International Journal of Artificial Organs.2022; 45(10): 865. CrossRef
High Activity of N-Acetylcysteine in Combination with Beta-Lactams against Carbapenem-Resistant Klebsiella pneumoniae and Acinetobacter baumannii
Massimiliano De Angelis, Maria T. Mascellino, Maria C. Miele, Dania Al Ismail, Marisa Colone, Annarita Stringaro, Vincenzo Vullo, Mario Venditti, Claudio M. Mastroianni, Alessandra Oliva
Antibiotics.2022; 11(2): 225. CrossRef
Combination Therapies for Biofilm Inhibition and Eradication: A Comparative Review of Laboratory and Preclinical Studies
Sophia Hawas, Anthony D. Verderosa, Makrina Totsika
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Impact of N-acetylcysteine (NAC) and calcium hydroxide intracanal medications in primary endodontic infection: a randomized clinical trial
Frederico C. Martinho, Bruna J. M. Corazza, Rayana D. Khoury, Esteban I. F. Orozco, Cassia C. Toia, Felipe P. Machado, Marcia C. Valera
Clinical Oral Investigations.2022; 27(2): 817. CrossRef
In vitro Characterization of Biofilm Formation in Prevotella Species
Shurooq Zakariya Albaghdadi, Jenan Bader Altaher, Hana Drobiova, Radhika G. Bhardwaj, Maribasappa Karched
Frontiers in Oral Health.2021;[Epub] CrossRef
N-Acetyl Cysteine Modulates the Inflammatory and Oxidative Stress Responses of Rescued Growth-Arrested Dental Pulp Microtissues Exposed to TEGDMA in ECM
Gili Kaufman, Drago Skrtic
International Journal of Molecular Sciences.2020; 21(19): 7318. CrossRef
Effects of N-acetylcysteine on root resorption after tooth replantation
Mitsuhiko Nishimi, Koichi Nakamura, Akina Hisada, Kazuki Endo, Shuya Ushimura, Yoshitaka Yoshimura, Yasutaka Yawaka
Pediatric Dental Journal.2020; 30(2): 72. CrossRef
Fungistatic Action of N-Acetylcysteine on Candida albicans Biofilms and Its Interaction with Antifungal Agents
Thaís Soares Bezerra Santos Nunes, Leticia Matheus Rosa, Yuliana Vega-Chacón, Ewerton Garcia de Oliveira Mima
Microorganisms.2020; 8(7): 980. CrossRef
Facile preparation of mussel-inspired antibiotic-decorated titanium surfaces with enhanced antibacterial activity for implant applications
Jae Seo Lee, Sang Jin Lee, Seok Bin Yang, Donghyun Lee, Haram Nah, Dong Nyoung Heo, Ho-Jin Moon, Yu-Shik Hwang, Rui L. Reis, Ji-Hoi Moon, Il Keun Kwon
Applied Surface Science.2019; 496: 143675. CrossRef
Effects of Sodium Tripolyphosphate on Oral Commensal and Pathogenic Bacteria
JI-HOI MOON, MI HEE NOH, EUN-YOUNG JANG, SEOK BIN YANG, SANG WOOK KANG, KYU HWAN KWACK, JAE-IN RYU, JIN-YONG LEE
Polish Journal of Microbiology.2019; 68(2): 263. CrossRef
Subgingival microbiome of experimental gingivitis: shifts associated with the use of chlorhexidine andN-acetyl cysteine mouthwashes
Ahlam Al-Kamel, Divyashri Baraniya, Wadhah Abdulnaser Al-Hajj, Esam Halboub, Saleem Abdulrab, Tsute Chen, Nezar Noor Al-Hebshi
Journal of Oral Microbiology.2019; 11(1): 1608141. CrossRef
Antimicrobial and Antibiofilm N-acetyl-L-cysteine Grafted Siloxane Polymers with Potential for Use in Water Systems
Dorota Kregiel, Anna Rygala, Beata Kolesinska, Maria Nowacka, Agata S. Herc, Anna Kowalewska
International Journal of Molecular Sciences.2019; 20(8): 2011. CrossRef
pH-Responsive mineralized nanoparticles for bacteria-triggered topical release of antibiotics
Kyung Hyun Min, Eun-Young Jang, Hong Jae Lee, Yu-Shik Hwang, Jae-In Ryu, Ji-Hoi Moon, Sang Cheon Lee
Journal of Industrial and Engineering Chemistry.2019; 71: 210. CrossRef
N-acetyl cysteine versus chlorhexidine mouthwashes in prevention and treatment of experimental gingivitis: a randomized, triple-blind, placebo-controlled clinical trial
Ahlam Al-Kamel, Wadhah Abdulnaser Al-Hajj, Esam Halboub, Saleem Abdulrab, Khaled Al-Tahami, Nezar Noor Al-Hebshi
Clinical Oral Investigations.2019; 23(10): 3833. CrossRef
Antibacterial effects of sodium tripolyphosphate againstPorphyromonasspecies associated with periodontitis of companion animals
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Journal of Veterinary Science.2019;[Epub] CrossRef
Removal and killing of multispecies endodontic biofilms by N -acetylcysteine
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Biological Activities and Potential Oral Applications of N‐Acetylcysteine: Progress and Prospects
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Antibacterial effects of N-acetylcysteine against endodontic pathogens
Ji-Hoi Moon, Young-Suk Choi, Hyeon-Woo Lee, Jung Sun Heo, Seok Woo Chang, Jin-Yong Lee
Journal of Microbiology.2016; 54(4): 322. CrossRef
In Vitro Effects of Polyphosphate against Prevotella intermedia in Planktonic Phase and Biofilm
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Journal Article

Molecular Identification of Fecal Pollution Sources in Water Supplies by Host-Specific Fecal DNA Markers and Terminal Restriction Fragment Length Polymorphism Profiles of 16S rRNA Gene: Ju-Yong Jeong , Kyung-Ik Gil , Kyong-Hee Lee , Jong-Ok Ka; J. Microbiol. 2008;46(6):599-607. Published online December 24, 2008; DOI: https://doi.org/10.1007/s12275-008-0174-3

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Abstract: Specific fecal DNA markers were investigated for major pollution sources, cow, human, and pig, and occurrence of the identified markers was analyzed in river waters using Terminal Restriction Fragment Length Polymorphism (T-RFLP) techniques and sequencing of 16S rDNA of Bacteroides-Prevotella. The unique and specific DNA markers for cow and human were identified as a 222 bp and 60 bp peak in HaeIII T-RFLP profiles, respectively, and the pig-specific marker was not identified but the unique T-RFLP profile of pig could be used as a substitution. Human-specific marker was detected in most of the river waters tested (92.1%) and T-RFLP profiles of river waters were shown to be similar to those of human feces. Cluster analysis of T-RFLP data showed that the fecal sources were multiple (human plus cow and human plus dairy cow) in most of the river waters. The phylogenetic analysis for the clones recovered from the fecal and water samples showed that the clones from cow formed a discreet cluster from those of other sources. The other clones from human, pig, and river water formed two groups all together. The results of this study could be used to identify and control the fecal pollution source in the bodies of water in Korea.

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