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- Bile salt hydrolase-mediated inhibitory effect of Bacteroides ovatus on growth of Clostridium difficile
- Soobin Yoon , Junsun Yu , Andrea McDowell , Sung Ho Kim , Hyun Ju You , GwangPyo Ko
- J. Microbiol. 2017;55(11):892-899. Published online October 27, 2017
- DOI: https://doi.org/10.1007/s12275-017-7340-4
- 53 View
- 0 Download
- 29 Crossref
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Abstract
-
Clostridium difficile infection (CDI) is one of the most common
nosocomial infections. Dysbiosis of the gut microbiota
due to consumption of antibiotics is a major contributor to
CDI. Recently, fecal microbiota transplantation (FMT) has
been applied to treat CDI. However, FMT has important limitations
including uncontrolled exposure to pathogens and
standardization issues. Therefore, it is necessary to evaluate
alternative treatment methods, such as bacteriotherapy, as
well as the mechanism through which beneficial bacteria inhibit
the growth of C. difficile. Here, we report bile acid-mediated
inhibition of C. difficile by Bacteroides strains which
can produce bile salt hydrolase (BSH). Bacteroides strains
are not commonly used to treat CDI; however, as they comprise
a large proportion of the intestinal microbiota, they can
contribute to bile acid-mediated inhibition of C. difficile. The
inhibitory effect on C. difficile growth increased with increasing
bile acid concentration in the presence of Bacteroides
ovatus SNUG 40239. Furthermore, this inhibitory effect on
C. difficile growth was significantly attenuated when bile acid
availability was reduced by cholestyramine, a bile acid sequestrant.
The findings of this study are important due to
the discovery of a new bacterial strain that in the presence
of available bile acids inhibits growth of C. difficile. These
results
will facilitate development of novel bacteriotherapy strategies to control CDI. -
Citations
Citations to this article as recorded by
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Zixing Dong, Shuangshuang Yang, Cunduo Tang, Dandan Li, Yunchao Kan, Lunguang Yao
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Wenweiran Li, Hui Chen, Jianguo Tang
Pathogens.2024; 13(8): 702. CrossRef - Rational Design of Live Biotherapeutic Products for the Prevention of Clostridioides difficile Infection
Shanlin Ke, Javier A Villafuerte Gálvez, Zheng Sun, Yangchun Cao, Nira R Pollock, Xinhua Chen, Ciarán P Kelly, Yang-Yu Liu
The Journal of Infectious Diseases.2024;[Epub] CrossRef - Biofilm Formation of Clostridioides difficile, Toxin Production and Alternatives to Conventional Antibiotics in the Treatment of CDI
Leon M. T. Dicks
Microorganisms.2023; 11(9): 2161. CrossRef - Bile Salt Hydrolases with Extended Substrate Specificity Confer a High Level of Resistance to Bile Toxicity on Atopobiaceae Bacteria
Kana Morinaga, Hiroyuki Kusada, Hideyuki Tamaki
International Journal of Molecular Sciences.2022; 23(18): 10980. CrossRef - Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans
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- New insights into microbial bile salt hydrolases: from physiological roles to potential applications
- Optimization of Water Absorbing Exopolysaccharide Production on Local Cheap Substrates by Bacillus Strain CMG1403 Using One Variable at a Time Approach
- Muhammadi , Muhammad Afzal
- J. Microbiol. 2014;52(1):44-52. Published online January 4, 2014
- DOI: https://doi.org/10.1007/s12275-014-2622-6
- 54 View
- 0 Download
- 9 Crossref
-
Abstract
- Optimum culture conditions, and carbon and nitrogen sources for production of water absorbing exopolysaccharide by Bacillus strain CMG1403 on local cheap substrates were determined using one variable at a time approach. Carbon source was found to be sole substrate for EPS biosynthesis in the presence of yeast extract that supported the growth only and hence, indirectly enhanced the EPS yield. Whereas, urea only coupled with carbon source could enhance the EPS production but no effect on growth. The maximum yield of EPS was obtained when Bacillus strain CMG1403 was grown statically in neutral minimal medium with 25% volumetric aeration at 30°C for 10 days. Under these optimum conditions, a maximum yield of 2.71±0.024, 3.82±0.005, 4.33±0.021, 4.73±0.021, 4.85±0.024, and 5.52±0.016 g/L culture medium was obtained with 20 g (sugar) of sweet whey, glucose, fructose, sucrose, cane molasses and sugar beet the most efficient one respectively as carbon sources. Thus, the present study showed that under optimum culture conditions, the local cheap substrates could be superior and efficient alternatives to synthetic carbon sources providing way for an economical production of water absorbing EPS by indigenous soil bacterium Bacillus strain CMG1403.
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Citations
Citations to this article as recorded by- Importancia de las bacterias ácido lácticas como productoras de exopolisacáridos
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Bioresource Technology.2016; 213: 21. CrossRef - Alginate Production from Alternative Carbon Sources and Use of Polymer Based Adsorbent in Heavy Metal Removal
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