MSK
Search
- Page Path
- HOME > Search
Journal Articles
- Novosphingobium sp. PP1Y as a novel source of outer membrane vesicles
- Federica De Lise , Francesca Mensitieri , Giulia Rusciano , Fabrizio Dal Piaz , Giovanni Forte , Flaviana Di Lorenzo , Antonio Molinaro , Armando Zarrelli , Valeria Romanucci , Valeria Cafaro , Antonio Sasso , Amelia Filippelli , Alberto Di Donato , Viviana Izzo
- J. Microbiol. 2019;57(6):498-508. Published online May 27, 2019
- DOI: https://doi.org/10.1007/s12275-019-8483-2
- 63 View
- 0 Download
- 4 Web of Science
- 4 Crossref
-
Abstract
- Outer membrane vesicles (OMVs) are nanostructures of 20– 200 nm diameter deriving from the surface of several Gramnegative bacteria. OMVs are emerging as shuttles involved in several mechanisms of communication and environmental adaptation. In this work, OMVs were isolated and characterized from Novosphingobium sp. PP1Y, a Gram-negative non-pathogenic microorganism lacking LPS on the outer membrane surface and whose genome was sequenced and annotated. Scanning electron microscopy performed on samples obtained from a culture in minimal medium highlighted the presence of PP1Y cells embedded in an extracellular matrix rich in vesicular structures. OMVs were collected from the exhausted growth medium during the mid-exponential phase, and purified by ultracentrifugation on a sucrose gradient. Atomic force microscopy, dynamic light scattering and nanoparticle tracking analysis showed that purified PP1Y OMVs had a spherical morphology with a diameter of ca. 150 nm and were homogenous in size and shape. Moreover, proteomic and fatty acid analysis of purified OMVs revealed a specific biochemical “fingerprint”, suggesting interesting details concerning their biogenesis and physiological role. Moreover, these extracellular nanostructures do not appear to be cytotoxic on HaCaT cell line, thus paving the way to their future use as novel drug delivery systems.
-
Citations
Citations to this article as recorded by
-
Proteomic analysis of meropenem-induced outer membrane vesicles released by carbapenem-resistant
Klebsiella pneumoniae
Fangfang Fan, Guangzhang Chen, Siqian Deng, Li Wei, Mariola J. Ferraro
Microbiology Spectrum.2024;[Epub] CrossRef - LuxR402 of Novosphingobium sp. HR1a regulates the correct configuration of cell envelopes
Ana Segura, Lázaro Molina
Frontiers in Microbiology.2023;[Epub] CrossRef - Genomic and physiological characterization of Novosphingobium terrae sp. nov., an alphaproteobacterium isolated from Cerrado soil containing a mega-sized chromid
Aline Belmok, Felipe Marques de Almeida, Rodrigo Theodoro Rocha, Carla Simone Vizzotto, Marcos Rogério Tótola, Marcelo Henrique Soller Ramada, Ricardo Henrique Krüger, Cynthia Maria Kyaw, Georgios J. Pappas
Brazilian Journal of Microbiology.2023; 54(1): 239. CrossRef - Outer Membrane Vesicles Derived from Klebsiella pneumoniae Are a Driving Force for Horizontal Gene Transfer
Federica Dell’Annunziata, Carmela Dell’Aversana, Nunzianna Doti, Giuliana Donadio, Fabrizio Dal Piaz, Viviana Izzo, Anna De Filippis, Marilena Galdiero, Lucia Altucci, Giovanni Boccia, Massimiliano Galdiero, Veronica Folliero, Gianluigi Franci
International Journal of Molecular Sciences.2021; 22(16): 8732. CrossRef
-
Proteomic analysis of meropenem-induced outer membrane vesicles released by carbapenem-resistant
Klebsiella pneumoniae
- Proteomic characterization of the outer membrane vesicle of the halophilic marine bacterium Novosphingobium pentaromativorans US6-1
- Sung Ho Yun , Sang-Yeop Lee , Chi-Won Choi , Hayoung Lee , Hyun-Joo Ro , Sangmi Jun , Yong Min Kwon , Kae Kyoung Kwon , Sang-Jin Kim , Gun-Hwa Kim , Seung Il Kim
- J. Microbiol. 2017;55(1):56-62. Published online December 30, 2016
- DOI: https://doi.org/10.1007/s12275-017-6581-6
- 52 View
- 0 Download
- 17 Crossref
-
Abstract
- Novosphingobium pentaromativorans US6-1 is a Gram-negative halophilic marine bacterium able to utilize several polycyclic aromatic hydrocarbons such as phenanthrene, pyrene, and benzo[a]pyrene. In this study, using transmission electron microscopy, we confirmed that N. pentaromativorans US6-1 produces outer membrane vesicles (OMVs). N. pentaromativorans OMVs (hereafter OMVNovo) are spherical in shape, and the average diameter of OMVNovo is 25–70 nm. Proteomic analysis revealed that outer membrane proteins and periplasmic proteins of N. pentaromativorans are the major protein components of OMVNovo. Comparative proteomic analysis with the membrane-associated protein fraction and correlation analysis demonstrated that the outer membrane proteins of OMVNovo originated from the membrane- associated protein fraction. To the best of our knowledge, this study is the first to characterize OMV purified from halophilic marine bacteria.
-
Citations
Citations to this article as recorded by- Bacterial extracellular vesicles: Vital contributors to physiology from bacteria to host
Xinke Nie, Qiqiong Li, Xinyang Chen, Stanley Onyango, Junhua Xie, Shaoping Nie
Microbiological Research.2024; 284: 127733. CrossRef - Marine Delivery Vehicles: Molecular Components and Applications of Bacterial Extracellular Vesicles
Angela Casillo, Raffaele D’Amico, Rosa Lanzetta, Maria Michela Corsaro
Marine Drugs.2024; 22(8): 363. CrossRef - Impact of probiotics-derived extracellular vesicles on livestock gut barrier function
Yuhan Zhang, Mengzhen Song, Jinping Fan, Xuming Guo, Shiyu Tao
Journal of Animal Science and Biotechnology.2024;[Epub] CrossRef - Genomic and physiological characterization of Novosphingobium terrae sp. nov., an alphaproteobacterium isolated from Cerrado soil containing a mega-sized chromid
Aline Belmok, Felipe Marques de Almeida, Rodrigo Theodoro Rocha, Carla Simone Vizzotto, Marcos Rogério Tótola, Marcelo Henrique Soller Ramada, Ricardo Henrique Krüger, Cynthia Maria Kyaw, Georgios J. Pappas
Brazilian Journal of Microbiology.2023; 54(1): 239. CrossRef - The relationship between bacterial outer membrane vesicles and halophilic adaptation
Dilan Barut, Blaise M. Enuh, Burak Derkuş, Ülkü Güler, Bekir Salih, Pınar Aytar Çelik
Molecular Omics.2023; 19(2): 174. CrossRef - Proteomic and Functional Analyses of Outer Membrane Vesicles Secreted by Vibrio splendidus
Huimin Song, Yilong Ruan, Ya Li, Huirong Yang, Weiwei Zhang
Journal of Ocean University of China.2023; 22(5): 1361. CrossRef - Bacterial membrane vesicle functions, laboratory methods, and applications
Pınar Aytar Çelik, Burak Derkuş, Kübra Erdoğan, Dilan Barut, Enuh Blaise Manga, Yalın Yıldırım, Simon Pecha, Ahmet Çabuk
Biotechnology Advances.2022; 54: 107869. CrossRef - Prochlorococcus extracellular vesicles: molecular composition and adsorption to diverse microbes
Steven J. Biller, Rachel A. Lundeen, Laura R. Hmelo, Kevin W. Becker, Aldo A. Arellano, Keven Dooley, Katherine R. Heal, Laura T. Carlson, Benjamin A. S. Van Mooy, Anitra E. Ingalls, Sallie W. Chisholm
Environmental Microbiology.2022; 24(1): 420. CrossRef - Benzo[a]pyrene might be transported by a TonB-dependent transporter in Novosphingobium pentaromativorans US6-1
Jiaqing Liang, Jiantao Xu, Weijun Zhao, Jiaofeng Wang, Kai Chen, Yuqian Li, Yun Tian
Journal of Hazardous Materials.2021; 404: 124037. CrossRef - Isolation and Characterization of Outer Membrane Vesicles of Pectobacterium brasiliense 1692
Silindile Maphosa, Lucy Novungayo Moleleki
Microorganisms.2021; 9(9): 1918. CrossRef - Eco-evolutionary feedbacks mediated by bacterial membrane vesicles
Nikola Zlatkov, Aftab Nadeem, Bernt Eric Uhlin, Sun Nyunt Wai
FEMS Microbiology Reviews.2021;[Epub] CrossRef - Analysis of the Extracellular Proteome of Colistin-Resistant Korean Acinetobacter baumannii Strains
Sang-Yeop Lee, Sung Ho Yun, Hayoung Lee, Yoon-Sun Yi, Edmond Changkyun Park, Wooyoung Kim, Hye-Yeon Kim, Je Chul Lee, Gun-Hwa Kim, Seung Il Kim
ACS Omega.2020; 5(11): 5713. CrossRef - Comprehensive proteomic analysis and pathogenic role of membrane vesicles of Listeria monocytogenes serotype 4b reveals proteins associated with virulence and their possible interaction with host
Raman Karthikeyan, Pratapa Gayathri, Paramasamy Gunasekaran, Medicharla V. Jagannadham, Jeyaprakash Rajendhran
International Journal of Medical Microbiology.2019; 309(3-4): 199. CrossRef - Novosphingobium sp. PP1Y as a novel source of outer membrane vesicles
Federica De Lise, Francesca Mensitieri, Giulia Rusciano, Fabrizio Dal Piaz, Giovanni Forte, Flaviana Di Lorenzo, Antonio Molinaro, Armando Zarrelli, Valeria Romanucci, Valeria Cafaro, Antonio Sasso, Amelia Filippelli, Alberto Di Donato, Viviana Izzo
Journal of Microbiology.2019; 57(6): 498. CrossRef - Proteomic and Metabolomic Analyses of Xylella fastidiosa OMV-Enriched Fractions Reveal Association with Virulence Factors and Signaling Molecules of the DSF Family
Oséias R. Feitosa-Junior, Eliezer Stefanello, Paulo A. Zaini, Rafael Nascimento, Paulo M. Pierry, Abhaya M. Dandekar, Steven E. Lindow, Aline M. da Silva
Phytopathology®.2019; 109(8): 1344. CrossRef - Biophysical restriction of growth area using a monodispersed gold sphere nanobarrier prolongs the mitotic phase in HeLa cells
Dae-Woong Jung, Hyun-Joo Ro, Junmin Kim, Seung Il Kim, Gi-Ra Yi, Gaehang Lee, Sangmi Jun
RSC Advances.2019; 9(64): 37497. CrossRef - Extracellular membrane vesicles in the three domains of life and beyond
Sukhvinder Gill, Ryan Catchpole, Patrick Forterre
FEMS Microbiology Reviews.2019; 43(3): 273. CrossRef
- Bacterial extracellular vesicles: Vital contributors to physiology from bacteria to host
First
Prev
TOP
