Journal of Microbiology

Journal Articles

Effects of Light and Dark Conditions on the Transcriptome of Aging Cultures of Candidatus Puniceispirillum marinum IMCC1322: Ji Hyen Lee, Hyun-Myung Oh; J. Microbiol. 2024;62(4):297-314. Published online April 25, 2024; DOI: https://doi.org/10.1007/s12275-024-00125-0

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To elucidate the function of proteorhodopsin in Candidatus Puniceispirillum marinum strain IMCC1322, a cultivated representative of SAR116, we produced RNA-seq data under laboratory conditions. We examined the transcriptomes of six different cultures, including sets of expression changes under constant dark (DD), constant light (LL), and diel-cycled (LD; 14 h light: 10 h dark) conditions at the exponential and stationary/death phases. Prepared mRNA extracted from the six samples was analyzed on the Solexa Genome Analyzer with 36 cycles. Differentially expressed genes on the IMCC1322 genome were distinguished as four clusters by K-mean clustering and each CDS (n = 2546) was annotated based on the KEGG BRITE hierarchy. Cluster 0 (n = 1573) covered most constitutive genes including proteorhodopsin, retinoids, and glycolysis/TCA cycle. Cluster 1 genes (n = 754) were upregulated in stationary/death phase under constant dark conditions and included genes associated with bacterial defense, membrane transporters, nitrogen metabolism, and senescence signaling. Cluster 2 genes (n = 197) demonstrated upregulation in exponential phase cultures and included genes involved in genes for oxidative phosphorylation, translation factors, and transcription machinery. Cluster 3 (n = 22) contained light-stimulated upregulated genes expressed under stationary/phases. Stringent response genes belonged to cluster 2, but affected genes spanned various cellular processes such as amino acids, nucleotides, translation, transcription, glycolysis, fatty acids, and cell wall components. The coordinated expression of antagonistic stringent genes, including mazG, ppx/gppA, and spoT/relA may provide insight into the controlled cultural response observed between constant light and constant dark conditions in IMCC1322 cultures, regardless of cell numbers and biomass.

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Effect of Light Regime on Candidatus Puniceispirillum marinum IMCC1322 in Nutrient-Replete Conditions
Hyun-Myung Oh, Ji Hyen Lee, Ahyoung Choi, Sung-Hyun Yang, Gyung-Hoon Shin, Sung Gyun Kang, Jang-Cheon Cho, Hak Jun Kim, Kae-Kyoung Kwon
Journal of Microbiology and Biotechnology.2024;[Epub] CrossRef

Lactobacillus crispatus and its enolase and glutamine synthetase influence interactions between Neisseria gonorrhoeae and human epithelial cells: Jagoda Płaczkiewicz , Paulina Chmiel , Ewelina Malinowska , Pawel B&# , Agnieszka Kwiatek; J. Microbiol. 2020;58(5):405-414. Published online April 11, 2020; DOI: https://doi.org/10.1007/s12275-020-9505-9

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Abstract

Neisseria gonorrhoeae, an obligatory human pathogen causes the sexually transmitted disease gonorrhea, which remains a global health problem. N. gonorrhoeae primarily infects the mucosa of the genitourinary tract, which in women, is colonized by natural microbiota, dominated by Lactobacillus spp., that protect human cells against pathogens. In this study, we demonstrated that precolonization of human epithelial cells with Lactobacillus crispatus, one of the most prevalent bacteria in the female urogenital tract, or preincubation with the L. crispatus enolase or glutamine synthetase impairs the adhesion and invasiveness of N. gonorrhoeae toward epithelial cells, two crucial steps in gonococcal pathogenesis. Furthermore, decreased expression of genes encoding the proinflammatory cytokines, TNFα and CCL20, which are secreted as a consequence of N. gonorrhoeae infection, was observed in N. gonorrhoeae-infected epithelial cells that had been precolonized with L. crispatus or preincubated with enolase and glutamine synthetase. Thus, our results indicate that the protection of human cells against N. gonorrhoeae infection is a complex process and that L. crispatus and its proteins enolase and glutamine synthetase can have a potential role in protecting epithelial cells against gonococcal infection. Therefore, these results are important since disturbances of the microbiota or of its proteins can result in dysbiosis, which is associated with increased susceptibility of epithelium to pathogens.

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Citations to this article as recorded by

Probiotics: Health benefits, food application, and colonization in the human gastrointestinal tract
Li Ying Jessie Lau, Siew Young Quek
Food Bioengineering.2024; 3(1): 41. CrossRef
Use of probiotic lactobacilli in the treatment of vaginal infections: In vitro and in vivo investigations
Peng Liu, Yune Lu, Rongguo Li, Xiaodi Chen
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef
Enhanced IgA coating of bacteria in women with Lactobacillus crispatus-dominated vaginal microbiota
Annelot C. Breedveld, Heleen J. Schuster, Robin van Houdt, Rebecca C. Painter, Reina E. Mebius, Charlotte van der Veer, Sylvia M. Bruisten, Paul H. M. Savelkoul, Marjolein van Egmond
Microbiome.2022;[Epub] CrossRef
Molecular Regulatory Mechanisms Drive Emergent Pathogenetic Properties of Neisseria gonorrhoeae
Ashwini Sunkavalli, Ryan McClure, Caroline Genco
Microorganisms.2022; 10(5): 922. CrossRef
Both Neisseria gonorrhoeae and Neisseria sicca Induce Cytokine Secretion by Infected Human Cells, but Only Neisseria gonorrhoeae Upregulates the Expression of Long Non-Coding RNAs
Jagoda Płaczkiewicz, Monika Adamczyk-Popławska, Ewa Kozłowska, Agnieszka Kwiatek
Pathogens.2022; 11(4): 394. CrossRef
Role of the human vaginal microbiota in the regulation of inflammation and sexually transmitted infection acquisition: Contribution of the non-human primate model to a better understanding?
Cindy Adapen, Louis Réot, Elisabeth Menu
Frontiers in Reproductive Health.2022;[Epub] CrossRef
Alterations of Vaginal Microbiota in Women With Infertility and Chlamydia trachomatis Infection
Hongliang Chen, Li Wang, Lanhua Zhao, Lipei Luo, Shuling Min, Yating Wen, Wenbo Lei, Mingyi Shu, Zhongyu Li
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Lactobacillus Cell Surface Proteins Involved in Interaction with Mucus and Extracellular Matrix Components
Lidia Muscariello, Barbara De Siena, Rosangela Marasco
Current Microbiology.2020; 77(12): 3831. CrossRef

Research Support, Non-U.S. Gov'ts

Genotyping, Morphology and Molecular Characteristics of a Lytic Phage of Neisseria Strain Obtained from Infected Human Dental Plaque: Ahmed N Aljarbou , Mohamad Aljofan; J. Microbiol. 2014;52(7):609-618. Published online May 30, 2014; DOI: https://doi.org/10.1007/s12275-014-3380-1

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Abstract

The lytic bacteriaphage (phage) A2 was isolated from human dental plaques along with its bacterial host. The virus was found to have an icosahedron-shaped head (60±3 nm), a sheathed and rigid long tail (~175 nm) and was categorized into the family Siphoviridae of the order Caudovirales, which are dsDNA viral family, characterised by their ability to infect bacteria and are nonenveloped with a noncontractile tail. The isolated phage contained a linear dsDNA genome having 31,703 base pairs of unique sequence, which were sorted into three contigs and 12 single sequences. A latent period of 25 minutes and burst size of 24±2 particles was determined for the virus. Bioinformatics approaches were used to identify ORFs in the genome. A phylogenetic analysis confirmed the species inter-relationship and its placement in the family.

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The potential for bacteriophages and prophage elements in fighting and preventing the gonorrhea
Monika Adamczyk-Popławska, Piotr Golec, Andrzej Piekarowicz, Agnieszka Kwiatek
Critical Reviews in Microbiology.2024; 50(5): 769. CrossRef
Periodontitis: etiology, conventional treatments, and emerging bacteriophage and predatory bacteria therapies
Anna Łasica, Piotr Golec, Agnieszka Laskus, Magdalena Zalewska, Magdalena Gędaj, Magdalena Popowska
Frontiers in Microbiology.2024;[Epub] CrossRef
Screening of Anorectal and Oropharyngeal Samples Fails to Detect Bacteriophages Infecting Neisseria gonorrhoeae
Jolein Gyonne Elise Laumen, Saïd Abdellati, Sheeba Santhini Manoharan-Basil, Christophe Van Dijck, Dorien Van den Bossche, Irith De Baetselier, Tessa de Block, Surbhi Malhotra-Kumar, Patrick Soentjes, Jean-Paul Pirnay, Chris Kenyon, Maia Merabishvili
Antibiotics.2022; 11(2): 268. CrossRef
A novel phage from periodontal pockets associated with chronic periodontitis
Yu Zhang, Tong-Ling Shan, Fei Li, Tian Yu, Xi Chen, Xu-Tao Deng, Eric Delwart, Xi-Ping Feng
Virus Genes.2019; 55(3): 381. CrossRef
Identification of Novel Bacteriophages with Therapeutic Potential That Target Enterococcus faecalis
M. Al-Zubidi, M. Widziolek, E. K. Court, A. F. Gains, R. E. Smith, K. Ansbro, A. Alrafaie, C. Evans, C. Murdoch, S. Mesnage, C. W. I. Douglas, A. Rawlinson, G. P. Stafford, Marvin Whiteley
Infection and Immunity.2019;[Epub] CrossRef
Ecology of the Oral Microbiome: Beyond Bacteria
Jonathon L. Baker, Batbileg Bor, Melissa Agnello, Wenyuan Shi, Xuesong He
Trends in Microbiology.2017; 25(5): 362. CrossRef
The use of bacteriophages to biocontrol oral biofilms
Szymon P. Szafrański, Andreas Winkel, Meike Stiesch
Journal of Biotechnology.2017; 250: 29. CrossRef

Rapid One Step Detection of Pathogenic Bacteria in Urine with Sexually Transmitted Disease (STD) and Prostatitis Patient by Multiplex PCR Assay (mPCR): Sang Rok Lee , Ji Min Chung , Young Gon Kim; J. Microbiol. 2007;45(5):453-459.; DOI: https://doi.org/2590 [pii]

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Abstract: We developed a multiplex PCR (mPCR) assay to simultaneously detect Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Ureaplasma urealyticum, Corynebacterium spp. and seudomona aeruginosa. This method employs a single tube and multiple specific primers which yield 200, 281, 346, 423, 542, and 1,427 bp PCR products, respectively. All the PCR products were easily detected by agarose gel electrophoresis and were sequenced to confirm the specificity of the reactions. To test this method, DNA extracted from urine samples was collected from 96 sexually transmitted disease or prostatitis patients at a local hospital clinical center, and were subjected to the mPCR assay. The resulting amplicons were cloned and sequenced to exactly match the sequences of known pathogenic isolates. N. gonorrhoeae and Corynebacterium spp. were the most frequently observed pathogens found in the STDs and prostatitis patients, respectively. Unexpectedly, P. aeruginosa was also detected in some of the STD and prostatitis samples. More than one pathogen species was found in 10% and 80.7% of STD and prostatitis samples, respectively, indicating that STD and prostatitis patients may have other undiagnosed and associates. The sensitivity of the assay was determined by sing purified DNA from six pathogenic laboratory strains and revealed that this technique could detect pathogenic DNA at concentrations ranging from 0.018 to 1.899 pg/μl. Moreover, the specificities of this assay were found to be highly efficient. Thus, this mPCR assay may be useful for the rapid diagnosis of causative infectious STDs and prostatitis. useful for the infectious STDs and prostatitis.

Journal Articles

The Molecular Characterization of Serogroup C Neisseria meningitidis Strains Circulating in Beijing: Tie-gang Zhang , Jing-guo He , Xiong He , Li-Juan Chen , Zhu-jun Shao , Mei-ping Sun; J. Microbiol. 2006;44(6):685-688.; DOI: https://doi.org/2455 [pii]

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Abstract: The aim of this study was to characterize the molecular features of serogroup C Neisseria meningitidis strains circulating in Beijing, China. Twenty out of 23 strains belonged to ST 4821. The causative serosubtype for meningococcal meningitis was P1.12-1,16-8. All of the strains expressed class 3 PorB protein. Among the five pulsed-field gel electrophoresis patterns observed, pattern III predominated.

Laboratory Confirmation of A Suspicious Meningococcal Meningitis Death Case: Tie-gang Zhang , Xiong He , Li-juan Chen , Jing-guo He , Ming Luo , Jie Yang , Zhu-jun Shao , Mei-ping Sun; J. Microbiol. 2006;44(4):457-460.; DOI: https://doi.org/2405 [pii]

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Abstract: A suspicious meningococcal meningitis death case was reported to the Beijing CDC. The blood specimen was analyzed via multi-PCR and MLST. 6 isolates from close contacts were analyzed via PFGE and MLST. According to the results of the above analyses, the cause of this case was identified as a serogroup A Neisseria meningitidis, which, in terms of sequence typing, belonged the ST7 group.

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